Background & Aim:Ethanol metabolism by the liver differentially impairs hepatic functions. This study investigated the attenuating potential of Abrus precatorius seeds on liver damage in HCl/EtOH induced rats and evaluated the prophylactic potential of ethylacetate More
Background & Aim:Ethanol metabolism by the liver differentially impairs hepatic functions. This study investigated the attenuating potential of Abrus precatorius seeds on liver damage in HCl/EtOH induced rats and evaluated the prophylactic potential of ethylacetate extract of A. precatorius on the hepatic function of acidified ethanol induced rats.Experimental: Rats were pre-treated with ethylacetate extract of A. precatorius seeds and standard drugs for eight consecutive days and 0.15M HCl/EtOH (60%) (1:1) was administered once on the 8th day. Biochemical assay, mRNA expression and liver histo-pathological studies were performed using standard procedures.Results: HCl/EtOH induction significantly (p <0.05) raised hepatic alanine amino transferase and aspartate amino transferase activities that were ameliorated by pre-treatment with 100 and 200mg/kg b.w of A. precatorius seeds extract. The significant (p <0.05) up-regulation of CYP2D3, CYP3A4 and GGT mRNA expression in the liver tissues of HCl/EtOH induced rats were successfully down-regulated upon pre-treatment with the extract. The micro-morphological alteration characterized by severe congestion of central venules and sinusoids, fibrosis at the portal veins and presence of inflammatory cells observed in HCl/EtOH induced rats was reversed by pre-treatment with A. precatorius seeds.Recommended applications/industries: Acidified ethanol used for ulcerative induction could result in liver injury in rats; pre-treatment with A. precatorius seeds extract could exert hepato-protective efficacy.
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Background & Aim:Diabetes mellitus is a metabolic disease that affects all systems in the body, including the liver. This study evaluated the in-vitro antioxidant capacity and liver function status of STZ-induced diabetic rats treated with petroleum and diethyl ethe More
Background & Aim:Diabetes mellitus is a metabolic disease that affects all systems in the body, including the liver. This study evaluated the in-vitro antioxidant capacity and liver function status of STZ-induced diabetic rats treated with petroleum and diethyl ether fractions of Blighia sapida stem barkfor 14 days. Experimental: The antioxidant ability of the petroleum and diethyl ether fraction of Blighia sapida stem bark was evaluated by total flavonoids and phenolic content (TFC and TPC) and DPPH scavenging activity using standard protocol. Thirty-five rats in seven groups were used. Plasma transaminases (ALT and AST) activities and bilirubin level was determined using standard procedure. Results: The TFC and TPC of petroleum ether fraction of B. sapida (PEFBS) (47.16 mg QUE/100g and 39.87 mg GAE/100g) was observed to be higher compared to diethyl ether fraction of B. sapida (DEFBS) (37.44 mg QUE/100 g and 36.74 mg/GAE/100g). The DPPH scavenging activity of the fractions were significantly (P < 0.05) reduced across the concentrations compared to the standard (gallic acid). STZ induced diabetes rats administered 2 ml/kg b. w. of normal saline significantly (p < 0.05) increased plasma ALT, AST activities and bilirubin level compared to the normal control rats while treatment of diabetic rats with petroleum and diethyl ether fraction of B. sapida at both dosesreduced the activities of these enzymes and level of bilirubin. Recommended applications/industries: The results sustain the fact that, the fractions of B. sapida have an immense potential to be developed further into a therapeutic agent.
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