-
Open Access Article
1 - Microfluidics and improving the cell culture channel: evaluation of spermatogonial stem cells using microfluidic chips
S. Naeemi A.M. Kajbafzadeh, A. Eidi, R. Khanbabaee, H. Sadri-ArdekaniThe main purpose of the present study was to investigate the treatment of male infertility, because infertility treatment is important in the group of cancer patients treated with gonadotoxic drugs. The main approach of the mentioned study is to compare two different gr MoreThe main purpose of the present study was to investigate the treatment of male infertility, because infertility treatment is important in the group of cancer patients treated with gonadotoxic drugs. The main approach of the mentioned study is to compare two different groups of spermatogonia stem cell culture methods and to evaluate the efficiency of differentiation and proliferation of these group of cells. Successful transplantation of spermatogonia stem cells (SSCs) in laboratory studies requires a suitable microenvironment for proliferation and differentiation of these cells. The natural extracellular matrix provides a good environment for stem cell culture. In the present study, themain purpose was to evaluate the ability of spermatogonial stem cells proliferation and differentiationvia the utilizing the microfluidic device (ex vivo). On the other hand, in the present study, we compared the obtained resultswith culture conditions in conventional culture plates (in vitro) to compare the SSCs proliferation and differentiation ability. In in-vitro culture method first spermatogonia stem cells from neonatal mice were isolated, then the resulted cells were seeded in culture plates on a scaffold consisting of hyaluronic acid, chitosan and decellularized testicular tissue, furthermore, in ex-vivo study the extracted spermatogonial stem cells were cultured in the microfluidic system without a scaffold. In ex vivo study, spermatogonial stem cells were extracted from neonatal male NMRI mice. The extracted cells were transferred to a microfluidic chip that was designed without an external pump, thereafter, the culture process was evaluated by IHC evaluation after one-month culture. In examined samples, cell attachment to the seminiferous tubules, DAPI staining and immunohistochemistry were evaluated. The results of immunohistochemical studies showed a significant increase in the expression of PLZF and TEKT1 markers in ex-vivo models. Finally, the results revealed that the ability of spermatogonia stem cells to induce spermatogenesis and production of haploid cells under testicular tissue culture in the microfluidic system is much more significant than conventional culture conditions in laboratory plates for these cells. Manuscript profile -
Open Access Article
2 - In-vitro Study on the Effect of Graphene Oxide and Calcium Phosphate on the Bone Scaffold Based on the Polycaprolactone and Polyhydroxybutyrate Valerate
V. Kheiri A. Asefnejad M.R. Noorani V. Goodarzi M.R. Kalaee -
Open Access Article
3 - Electrospinning of High Concentration Gelatin and Crosslinking Through the Millard Reaction for Use in Fabrication of Tissue Engineering Scaffolds
N. Zandi M.A. Shokrgozar E. Tamjid A.R. Simchi -
Open Access Article
4 - Mechanical Behavior of Porous Nanocomposite Polycaprolactone Scaffolds Containing Microwave Assisted Egg Shell-derived Nanohydroxyapatite
V. Rastgoui Haghi N. Najmoddin -
Open Access Article
5 - Surface Activation and Crystallographic Investigation of Nano-crystalline Alumina Scaffolds for Bone Tissue Engineering Applications
S. Joughehdoust A.A. Behnamghader M. Imani -
Open Access Article
6 - Qualitative Study of the Principles of Educational Design Based on Cognitive Flexibility Theory in Combination with Scaffolding Strategies
ABBAS TAGHIZADE javad hatami maryam ghasemiPresenting complex domains of knowledge through traditional linear models of instruction, is problematic because as content areas becomes more complex, the domain of knowledge becomes more ill structured, non-uniform, and overlapping with other domains. For this, Spiro MorePresenting complex domains of knowledge through traditional linear models of instruction, is problematic because as content areas becomes more complex, the domain of knowledge becomes more ill structured, non-uniform, and overlapping with other domains. For this, Spiro & et al. argue that learning environments should emphasize ‘‘the real-world complexity and ill-structuredness of many knowledge domains. The aim of this research was qualitative study of the principles of educational design based on cognitive flexibility theory in combination with scaffolding strategies in computer-based learning environments. Qualitative content analysis method was used in this research. The study population included all resources and related articles indexed in scientific databases during 1991-2015(84resource) related to the 2 theories of this study. A sample through Purposive sampling was selected and finally the proposed educational design model based on cognitive flexibility theory with 9 principles in combination with scaffolding strategies was presented. Since cognitive flexibility theory is generally presented for advanced knowledge acquirement, the proposed model can be used for educational design for learners at high school levels and upper. Manuscript profile -
Open Access Article
7 - The Effect ofCyanobacteriaNostoc . Sp ISC 113Polysaccharide on the Proliferation and Adhesion of Endothelial Cells to Repair the Vessel
ome foroh maleki Mahrouz DezfulianInroduction & ObjectiveThe applications of extracellular materials of marine organisms are widely expanded. Some of the secreted materials of cyanobacteria are polysaccharides. In the present study extracted polysaccharide of Nostoc. sp ISC 113 cyanobacteria evaluated a MoreInroduction & ObjectiveThe applications of extracellular materials of marine organisms are widely expanded. Some of the secreted materials of cyanobacteria are polysaccharides. In the present study extracted polysaccharide of Nostoc. sp ISC 113 cyanobacteria evaluated as cellular scaffolding on endothelial cellsMaterials and Methods:The polysaccharide extracted from cyanobacteria Nostoc.sp ISC 113. The cell scaffold made from polysaccharide with collagen and hyaluronic acid and its effects on the growth and proliferation assessed by MTT assay and the cell adhesion of HUVEC endothelial to scaffold evaluated, by crystal violet staining and microscopic studies. The colony assay performed for clonogenic endothelial cells.ResultsThe data obtained from MTT assay showed that the extracted polysaccharide of Nostoc cyanobacteria increased the proloiferation, adhesion, and clonogenic endothelial HUVEC cells.Conclusion: The extracted polysaccharide of Nostoc is appropriate natural polymer to build scaffold or repair the capillaries Manuscript profile -
Open Access Article
8 - The Study Of the effect Of ovine bladder scaffold Of sheep, ِDomesticated breed, on blastema cells in vitro
نگار صغیری هاشم راستی جواد بهارآرا ناصر مهدوی شهری مهدی مرجانی سید حسن علوی فاطمه علویIntroduction and ObjectiveBiological scaffolds, composed of Extra Cellular Matrix (ECM), are capable of facilitating the restructuring of a large number of tissues. The objective of this study was to investigate the effect of ovine bladder scaffold of sheep, domesticate MoreIntroduction and ObjectiveBiological scaffolds, composed of Extra Cellular Matrix (ECM), are capable of facilitating the restructuring of a large number of tissues. The objective of this study was to investigate the effect of ovine bladder scaffold of sheep, domesticated breedon blastema cells in vitroMaterials and Methods: At first, ovine bladder o the sheep was decellularized by putting in 1% wt/vol solution of sodium dodecyl sulfate (SDS) for 24 hours. Then to prepare the blastema tissue, the pinna of New Zealand rabbit was manually punched-hole with a special puncher and a circular blastema was separated. There after, the decellularized samples were put in the middle of blastema ring and were transfered to the culture medium. To continue the study, microscope with lights of hematoxylin and eosin and pick indigo and Toluidine blue staining was used. Some samples on the day 15 and 20 of culture were studied by transitional electron microscope.Results: Collagen fibers were preserved after decellularized in matrix of bladder. Most migration of blastema cells occurred on days 15 and 20 of culture. On the day 10 and 15, some immature cells were differentiation in the pathway of adipocyte and fibroblast. On the day 15th and 20th, more blastema cells were migrated to the ovine scaffold. On the 10th and 15 day immature cell culture was seen to be differentiated from fibroblast and adipocyte cells. On the 15 th day blastema cell culture differentiated from covering cells and on the 20th day from fibroblast and adipocyte cells were seen.Conclusion: Bladder scaffold has ability to induce blastema cells and causes not only blastoma cells’ migration but also to be changed to different cells. This phenomenon is very important because of no growth factor and inducer was used in culture medium. Manuscript profile -
Open Access Article
9 - اجرای روش حمایتی (داربستی) متقابل درمحیط یادگیری مجازی: مهارت های شنیداری و گفتاری زبان آموزان ایرانی زبان انگلیسی
Marzieh Sabzevari Neda Fatehi Rad Masoud Tajaddiniاین پژوهش تلاشی برای اجرا و بررسی روش حمایتی متقابل در محیط یادگیری مجازی زبان انگلیسی داشته است. به طور خاص، این تحقیق تأثیر روش آنلاین حمایتی متقابل بر مهارت های شنیداری و گفتاری زبان آموزان ایرانی را مورد تجزیه و تحلیل قرار داد. پس از اجرای آزمون تعیین سطح، 36 زبانآ Moreاین پژوهش تلاشی برای اجرا و بررسی روش حمایتی متقابل در محیط یادگیری مجازی زبان انگلیسی داشته است. به طور خاص، این تحقیق تأثیر روش آنلاین حمایتی متقابل بر مهارت های شنیداری و گفتاری زبان آموزان ایرانی را مورد تجزیه و تحلیل قرار داد. پس از اجرای آزمون تعیین سطح، 36 زبانآموز نمونه تحقیق را تشکیل دادند و به طور تصادفی به دو گروه آزمایشی (EG=18) که با روش مذکور و گروه کنترل (CG=18) که با استفاده از روشهای آموزشی سنتی آموزش داده شدند، طبقهبندی شدند. ابزارهای گردآوری داده ها شامل آزمون همگن سازی، پیش آزمون و پس آزمون در مهارت های شنیداری و گفتاری بود. این تحقیق در 16 جلسه انجام شد و اعضای گروه آزمایش در دوره های شنیداری و گفتاری خود آموزش راهبردهای روش حمایتی متقابل را دریافت کردند، در حالی که اعضای گروه کنترل این دو مهارت را بدون چنین آموزشی تمرین کردند. نتایج آزمونهای تی نمونههای مستقل و آزمونهای تی زوجی انجامشده در پیشآزمون و پسآزمون نشان داد که زبانآموزان گروه آزمایش در هر دو مهارت زبان از گروه کنترل بهتر عمل کردند. Manuscript profile -
Open Access Article
10 - داربست بندی مهارتهای شفاهی و نگرش در زبان انگلیسی با استفاده از طراحی نقشه ی داستان و پادپخش
Mohammed Pazhouhesh ضرغام قپانچیتحقیق حاضر به بررسی تاثیر طراحی نقشه ی داستان (story map) و پادپخش داستان(story podcast) به عنوان متغیرهای آزمایشی در ارتقائ مهارتهای شفاهی زبان انگلیسی به عنوان متغیر وابسته پرداخته است. تعداد 36 دانشجوی کارشناسی زبان انگلیسی درسه گروه در درس بیان شفاهی داستان تقسیم شد Moreتحقیق حاضر به بررسی تاثیر طراحی نقشه ی داستان (story map) و پادپخش داستان(story podcast) به عنوان متغیرهای آزمایشی در ارتقائ مهارتهای شفاهی زبان انگلیسی به عنوان متغیر وابسته پرداخته است. تعداد 36 دانشجوی کارشناسی زبان انگلیسی درسه گروه در درس بیان شفاهی داستان تقسیم شده و در تحقیقی شبه تجربی و در چهارچوب طرح تحقیقی موسوم به مربع لاتین( Latin Squared) شرکت کردند. تمامی شرکت کنندگان بترتیب در معرض سه آزمایشگر بازگویی صرف داستان، بازگویی داستان پس از تکمیل نقشه ی داستان، بازگویی داستان پس از شنیدن پادپخش آن قرار گرفتند. ارزشیابی شرکت کنندگان در پایان هر مرحله نشان از تاثیر معنی دار اثر آزمایشگر داستان گویی با استفاده از نقشه ی داستان و داستان گویی پس از شنیدن پادپخش داشت. عملکرد کلی در مهارتهای شفاهی زبانی پس از شنیدن فایل های صوتی داستان بهتر و معنی دارتراز دیگر عملکردهای شفاهی در گروهها بود. نظر سنجی پایانی نشان داد بسیاری از شرکت کنندگان با این فن آوریها آشنایی زیادی نداشتند و استفاده از این رسانه ها را برای رشد توانایی شفاهی زبانی و مهارت سخن گفتن مفید می دانستند. هم چنین استفاده از این رسانه ها را در دروسی مثل بیان شفاهی داستان برای پرورش بسندگی زبان انگلیسی و ایجاد نگرش مثبت کارآمد می دانستند. Manuscript profile -
Open Access Article
11 - تاثیر روش داربستی ژانر-محور بر روی نگارش مقالات تحقیقی زبان آموزان ایرانی
سارا صالح پور مهناز سعیدیدر سال های اخیر تحقیقات زیادی به بررسی آموزش نگارشی مبتنی برروش ژانر اختصاص داده شده است. با این وجود، استفاده از روش داربستی در زمینه نگارش آکادمیک توجه اندکی به خود جلب کرده است. از این رو، تحقیق حاضر در تلاشی برای مقابله با مشکلات رایج در نگارش آکادمیک، به بررسی  Moreدر سال های اخیر تحقیقات زیادی به بررسی آموزش نگارشی مبتنی برروش ژانر اختصاص داده شده است. با این وجود، استفاده از روش داربستی در زمینه نگارش آکادمیک توجه اندکی به خود جلب کرده است. از این رو، تحقیق حاضر در تلاشی برای مقابله با مشکلات رایج در نگارش آکادمیک، به بررسی تأتیر استفاده از روش داربستی مبتنی بر ژانر بوسیله آغازگر جمله ها و چارچوب های نگارشی بر عملکرد نگارشی دانشجویان فوق لیسانس آموزش زبان انگلیسی در حوزه مقاله نویسی می پردازد. برای این منظور 20 دانشجوی فوق لیسانس آموزش زبان انگلیسی به صورت تصادفی در دو گروه همگن آزمایش و کنترل که هر کدام شامل 10 نفر بودند قرار گرفتند. هر دو گروه یک دوره 5 جلسه ای آموزش نگارش به سبک ژانر شامل ساختارشکنی متن، ساخت وساز مشترک و ساخت و ساز مستقل متن بود را گذراندند. در حالیکه گروه آزمایشی، علتوه بر این از آغازگر جمله ها و چارچوب های نگارشی نیز بهره مند گردیدند. تجزیه و تحلیل نتایج با استفاده از آزمون مستقل تی نشان داد که آموزش نگارشی مبتنی بر سبک ژانر می تواند ابزار مفیدی در بهبود نگارش آکادمیک باشد. علاوه بر این، عملکرد بهتر گروه آزمایش نشانگر اتر مفید استفاده از روش داربستی توسط آغازگر جمله ها و چارچوب های نگارشی می باشد. Manuscript profile -
Open Access Article
12 - آموزش داربستی نگارش پیشرفته از طریق چهارچوبهای نوشتاری
سارا صالح پور Nasrin Hadidi بیوک بهنامیادگیری نگارش انگلیسی همواره مانع بسیار بزرگی برای زبان آموزان بوده است. در تلاشی جهت کاهش مشکلاتی که زبان اموزان انگلیسی در سطح پیشرفته در نگارش دارند تحقیق حاضر بر آن شد تا تاثیر آموزش داربستی را از طریق چهارچوبهای نگارش که از گروههای مبسوط واژگانی از پیش ساخته ایجاد Moreیادگیری نگارش انگلیسی همواره مانع بسیار بزرگی برای زبان آموزان بوده است. در تلاشی جهت کاهش مشکلاتی که زبان اموزان انگلیسی در سطح پیشرفته در نگارش دارند تحقیق حاضر بر آن شد تا تاثیر آموزش داربستی را از طریق چهارچوبهای نگارش که از گروههای مبسوط واژگانی از پیش ساخته ایجاد شده اند را بررسی کند. برای این منظور 40 زبان آموز دختر در سطح پیشرفته که از میان یک جمعیت 65 نفری انتخاب شده اند بطور تصادفی در دو گروه آزمایشی و کنترل قرار گرفتند. به شرکت کننگان در هر دو گروه یک پیش آزمون قبل از دادن هر گونه آموزشی به آنان و یک پس آزمون نگارش بعد از20 جلسه آموزش داربستی داده شد. نتایج نشان داد که شرکت کنندگان در گروه آموزشی بدلیل برخورداری از آموزش داربستی بهتر از گروه کنترل عمل کردند. روی هم رفته نتیجه گیری می شود که آموزش داربستی از طریق چهارچوبهای نگارش می تواند وسیله مفیدی برای کمک به زبان آموزان سطح پیشرفته در بهبود کیفیت نگارش آنها باشد. Manuscript profile -
Open Access Article
13 - آنلاین آزاد (MOOC) در مقابل برنامههای حضوری در رایتینگ آیلتس: ادراک آزمون دهندگان در کانون توجه
Rezvan Narimani Fariba Rahimi Esfahani Sajad Shafieeداربست در دورههای آنلاین آزاد عظیم (MOOC) در مقابل داربست در برنامههای حضوری در رایتینگ آیلتس: ادراکات آزموندهندگان در تمرکزاین تحقیق به دنبال بررسی ادراک شرکت کنندگان در آزمون آیلتس از دریافت آموزش مبتنی بر MOOC دارای داربست بود. برای دستیابی به این هدف، 50 داوطلب آ Moreداربست در دورههای آنلاین آزاد عظیم (MOOC) در مقابل داربست در برنامههای حضوری در رایتینگ آیلتس: ادراکات آزموندهندگان در تمرکزاین تحقیق به دنبال بررسی ادراک شرکت کنندگان در آزمون آیلتس از دریافت آموزش مبتنی بر MOOC دارای داربست بود. برای دستیابی به این هدف، 50 داوطلب آیلتس از طریق نمونه گیری آسان انتخاب شدند و به یکی از دو گروه تقسیم شدند: یک گروه آموزشی مبتنی بر MOOC ، (n = 25) و یک گروه آموزش حضوری (F2FG, n=25) شرکت کنندگان در آزمون MG در معرض یادگیری آینده قرار گرفتند، در حالی که به شرکت کنندگان آزمون F2FG شخصاً مطالب یکسان داده شد. داربست به شکل کمک های بصری، پیش آموزش اصطلاحات لازم، استفاده از دانش گذشته شرکت کنندگان در مورد یک موضوع، الگوبرداری از آنچه از آنها خواسته شده بود بنویسند، و ارائه الگوهای جملات مورد نیاز برای تکمیل آیلتس رایتینگ Task 2 بود. نمرات نوشتن پیش آزمون و پس آزمون دو گروه با استفاده از ANCOVA یک طرفه مورد بررسی قرار گرفت، که نشان داد یادگیرندگان MG به طور قابل توجهی از همتایان F2FG خود بهتر عمل کردند. نتایج پرسشنامه ادراک نشان داد که دانشجویان MG آموزش مبتنی بر MOOC را برای دورههای نوشتاری خود دوست داشتند. مفاهیم و کاربردهای نتایج پژوهش حاضر در پایان تحقیق ارائه شده است. Manuscript profile -
Open Access Article
14 - پژوهش کیفی تکنیک های روش حمایتی (داربستی) متقابل بر دیدگاه زبان آموزان ایرانی از طریق سیستم مدیریت زبان اِل اِم اِس
مرضیه سبزواری ندا فاتحی راد مسعود تاج الدینیاین پژوهش کیفی با هدف بررسی تأثیر تکنیکهای روش حمایتی (داربستی) متقابل در کلاسهای زبان انگلیسی بر دیدگاه زبانآموزان ایرانی در محیط سیستم مدیریت زبان اِل اِم اِس انجام شد. بدین منظور، 32 زبان آموز سطح متوسطه یکی از مؤسسات زبان کرمان به روش نمونهگیری تصادفی ساده Moreاین پژوهش کیفی با هدف بررسی تأثیر تکنیکهای روش حمایتی (داربستی) متقابل در کلاسهای زبان انگلیسی بر دیدگاه زبانآموزان ایرانی در محیط سیستم مدیریت زبان اِل اِم اِس انجام شد. بدین منظور، 32 زبان آموز سطح متوسطه یکی از مؤسسات زبان کرمان به روش نمونهگیری تصادفی ساده انتخاب شدند. ابزار گردآوری داده ها شامل آزمون همگن و مصاحبه های مرتبط بود. شرکت کنندگان، آموزش تکنیک های داربستی متقابل را در کلاس آنلاین خود از طریق اِل اِم اِس دریافت کردند. آنها راهبردهای آموزشی را که استقلال فراگیران را در محیط اِل اِم اِس تشویق می کرد، تمرین کردند. در پایان دوره با شرکت کنندگان بر اساس تکنیک اشباع داده ها مصاحبه شد. نتایج مصاحبه دیدگاه مثبت و منفی زبان آموزان را نسبت به استفاده از این استراتژی ها در محیط اِل اِم اِس بررسی کرد. نتایج نشان داد که استفاده از آموزش متقابل و داربستی و ادغام آنها فرصت های یادگیری بیشتری را در خصوص منابع یادگیری اضافی در چارچوب اِل اِم اِس ارائه می دهد. مراحل پایانی روش داربستی متقابل مربوط به استقلال و خودمختاری زبان آموزان بود، بنابراین آنها می توانستند منابع اضافی را بدون کمک گرفتن از معلم مطالعه کنند. در مجموع، همه عوامل منجر به رضایت بالای فراگیران از تکنیک ها شد. این پژوهش برخی مفاهیم آموزشی را برای معلمان و زبان آموزان در استفاده از تکنیک های داربستی متقابل در محیط یادگیری زبان خارجه پیشنهاد کرد. Manuscript profile -
Open Access Article
15 - Evaluation of adhession and viability of endometrial stem cells-derived osteoblast-like cells cultured on PLGA/HA scaffold
mojdeh salehi jafar ai nasrin lotfibakhshaiesh shiva irani سمیه ابراهیمی باروقIntroduction: The engineered tissue structure includes three components: cells, signaling pathway and scaffold. The polylactide-co-glycolide/hydroxyapatite (PLGA/HA) has attracted much attention due to their optimal properties because of using PLGA polymer and hydroxyap MoreIntroduction: The engineered tissue structure includes three components: cells, signaling pathway and scaffold. The polylactide-co-glycolide/hydroxyapatite (PLGA/HA) has attracted much attention due to their optimal properties because of using PLGA polymer and hydroxyapatite. The aim of this study was to evaluate the biocompatibility effect of PLGA/HA scafffold on osteoblast differentiated from hEnSCs. We aimed to evallute the adhesin of cells on scaffolds. hEnSCs were isolated from human endometrium tissue and induced the osteogenic differentiation, then the differentiated cells were cultured on PLGA/HA synthetic scaffolds. A nanocomposite scaffolds based on PLGA/HA were fabricated by electrospinning methode and behavior of differentiated osteoblast cells was evaluated after seeding cells on this scaffold. Osteogenesis was investigated in terms of attachment, alkaline phosphatase activity and gene expression. Morphogenic of cells in scaffold was evaluated by SEM and biocampatibility of this scaffold was confirmed by MTT assay. The alkaline phosphatase activity confirmed osteoblast differentiation. SEM studies showed that the surface properties of scaffold were desirable and the cells had the ability to attach and proliferate better on the nanocomposite scaffolds. It has been shown that the nanocomposite scaffolds have appropriate properties to support the attachment of differentiated osteoblast cells. Manuscript profile -
Open Access Article
16 - Fabrication of electrospun silk fibroin scaffold and the effect of its pre-incubation in culture medium on survival and adhesion of rat bone marrow mesenchymal cells
Maryam Janitermi esmail fattahi Seyed Gholam Ali JorsaraiObjective: This study was performed to synthesize electrospun silk fibroin scaffold and to investigate the time-dependent effect of its pre-incubation in culture medium on the adhesion and proliferation of cells seeded on the scaffold. Methods: sericin was removed from MoreObjective: This study was performed to synthesize electrospun silk fibroin scaffold and to investigate the time-dependent effect of its pre-incubation in culture medium on the adhesion and proliferation of cells seeded on the scaffold. Methods: sericin was removed from silk cocoon and fibrin solution (3% w/v) was prepared using formic acid. Then, electrospun silk fibroin scaffold was made using lab-scale electrospinning machine and evaluated by scanning electron microscopy. Pre-incubation of scaffolds in culture medium was performed for 0, 1, 6, and 10 days and the hydrophilicity of scaffolds was evaluated by measuring the water contact angle. Rat bone marrow mesenchymal cells were then isolated, cultured, and seeded on scaffolds. After 21 days of cell seeding, cell viability (MTT method) and genomic DNA concentration of cells attached to the scaffold were evaluated. Results: The results showed that increasing the pre-incubation time in the culture medium decreased the water contact angle and increased the survival and proliferation of cells. In general, the present study showed that pre-incubation of electrospun fibroin scaffold with constant elasticity in culture medium leads to increased scaffold hydrophilicity and consequently increases the proliferation and survival of mesenchymal cells seeded on it. Conclusion: these findings can be used as an effective factor in seeding cartilage cell on scaffolds in tissue engineering. Manuscript profile -
Open Access Article
17 - Experimental Study on Evaluation of Effects of Implantation Site of Mesenchymal Stem Cells/scaffold on Recovery of Diabetic in a Rat Model
elham hoveiziDiabetes Mellitus is an autoimmune and chronic disorder that has spread rapidly all over the world due to lifestyle and obesity. In this study, we are trying to find an effective step in controlling diabetes by designing an engineered tissue and grafting it to different MoreDiabetes Mellitus is an autoimmune and chronic disorder that has spread rapidly all over the world due to lifestyle and obesity. In this study, we are trying to find an effective step in controlling diabetes by designing an engineered tissue and grafting it to different sites in the animal model. Uterine endometrial mesenchymal stem cells (EnMSCs) were prepared using the enzymatic extraction method and PAN nanofiber scaffold by electrospinning method. EnMSCs were cultured on the scaffold and transplanted into diabetic rats treated with streptozotocin. The engineered tissue was transplanted in one group peritoneally in the abdominal cavity and the other group subcutaneously. Also, in another group of rats, EnMSCs were injected through the tail. After transplantation, blood glucose, insulin, and weight of rats were measured. The findings of the present study showed that the method and area of stem cell transplantation play an important role in the control of diabetes. In the groups receiving EnMSCs, glucose concentration, blood insulin level, and body weight were improved compared to the control group. Compared to other groups, glucose concentration decreased significantly and blood insulin level and body weight increased significantly in rats receiving a peritoneal transplant. In the subcutaneous transplant group and the injection group, there was no significant difference in the investigated criteria. According to the results of this study, transplantation of EnMSCs using PAN scaffold in the peritoneal site can be suggested for the treatment of diabetes, although more studies are needed in this field to provide a complete treatment. Manuscript profile -
Open Access Article
18 - Differentiation of amniotic membrane mesenchymal stem cells (hAMSCs) cardiomyocyte on PGS-co-PCL / PGC / PPy / Gelatin scaffold and its effect in the treatment of myocardial ischemia in adult male Wistar rats
mahsa Ale Ebrahim NASTARAN BAHRAMI nooshin barikrow yasin asadi fateme roholah ali salimiAbstractIntroduction: The cardiac ischemia and its subsequent failures have a high prevalence and its treatment is dangerous, expensive and ineffective. With the implant of mesenchymal stem cells, researchers hope to replace the dead tissues and reactivate the damaged p MoreAbstractIntroduction: The cardiac ischemia and its subsequent failures have a high prevalence and its treatment is dangerous, expensive and ineffective. With the implant of mesenchymal stem cells, researchers hope to replace the dead tissues and reactivate the damaged parts of the heart. Materials and methods: After the differentiation of mesenchymal stem cells of the amniotic membrane into heart muscle cells, by closing the left anterior descending coronary artery (LAD) for 45 minutes, acute ischemia was induced in male Wistar rats. Then reperfusion was performed in male rats. Then treatment groups, including: differentiated stem cells, stem cells with PGS-co-PCL/PGC/PPy/Gelatin scaffold and scaffold group, were implanted into the damaged heart. After 2 and 4 weeks, echocardiography was performed and angiogenesis was examined in the heart tissue using the immunohistochemical technique.Results and discussion: In treatment groups with mesenchymal cells and scaffolds + mesenchymal cells compared to the control, the amount of cardiac ejection fraction (EF), ejection fraction (FS) and cardiac stroke volume (SV) increases. Also, by examining the growth of VEGF in different groups and the control group, there was a significant increase in the expression of this angiogenic factor in the group receiving the scaffold + mesenchymal cells compared to other groups, which seems to use the PGS-co-PCL/PGC/PPy./Gel scaffold has created enough and suitable space for growth of vessels in the myocardium. Manuscript profile -
Open Access Article
19 - نانوکامپوزیت متخلخل هیدروکسی آپاتیت/تیتانیا برای کاربردهای بافت سخت
مهناز عنایتی جزی مهران صولتی هشجین علی نعمتی زهرا طاهریان -
Open Access Article
20 - طراحی داربست هیبریدی متخلخل برپایه چندسازه PEPC اصلاحشده با کیتوسان به منظور کاربرد در مهندسی بافت نرم: بررسی شباهت های ساختاری و رفتار زیست مکانیکی
آزاده ایزدیار آغمپونی مازیار شریف زاده بائی سعید حیدری کشل عظیم اکبرزاده خیاوی -
Open Access Article
21 - اثرات متقابل روش حمایتی (داربستی) بر مهارت های شفاهی زبان آموزان و خودکارآمدی آنها
Marziye Sabzevari Neda Fatehi Rad Massoud Tajaddiniتحقیق پیش رو با هدف بررسی تاثیرروش حمایتی ( داربستی) متقابل بر مهارت های شنیداری و گفتاری و سطوح خودکارآمدی آنها با استفاده از آموزش برخط (ان لاین) صورت گرفته است. در این تحقیق چهل و هشت زبان آموز دختر وپسر سطح پیش متوسطه ایرانی که رنج سنی آن ها بین شانزده تا بیست و دو Moreتحقیق پیش رو با هدف بررسی تاثیرروش حمایتی ( داربستی) متقابل بر مهارت های شنیداری و گفتاری و سطوح خودکارآمدی آنها با استفاده از آموزش برخط (ان لاین) صورت گرفته است. در این تحقیق چهل و هشت زبان آموز دختر وپسر سطح پیش متوسطه ایرانی که رنج سنی آن ها بین شانزده تا بیست و دو سال شرکت کردند. روش مطالعه ترکیبی همزمان برای انجام این تحقیق بکارگرفته شد. به منظور جمع آوری داد ه های کمی وکیفی از آزمون پت به عنوان پیش آزمون, پس آزمون، پرسشنامه مربوط به سطوح خودکارآمدی وهم چنین مصاحبه از فراگیران در انتهای ترم استفاده شده است. نتایج آزمونهای تی مستقل وتی زوجی نشان داد زبان آموزانی که در کلاسشان روش حمایتی (داربستی) متقابل اجرا شده بود (گروه آزمایش ) نتایج بهتری چه از نظر کمی وکیفی در مهارت های شنیداری و گفتاری بدست آوردند و سطوح خودکارآمدی بالاتری نسبت به زبان آموزان گروه کنترل بدست آوردند. همچنین بین خودکارآمدی و موفقیت در عملکرد گفتاری و شنیداری رابطه معناداری وجود داشت. می توان چنین استنباط کرد که راهبردهای روش حمایتی متقابل تأثیر مثبت معناداری بر بهبود مهارت های شفاهی دارند. در نهایت، نتایج مصاحبه نگرش مثبت و منفی زبان آموزان را نسبت به استفاده از این راهبردها در محیط LMS نشان داد. Manuscript profile -
Open Access Article
22 - اثرات بازخورد داربستی و اصلاحات صریح بر استفاده صحیح زبان آموزان ایرانی از حروف تعریف معین و نامعین انگلیسی در بیان شفاهی زبان آموزان
Lotfollah Samaee احسان رسایی محمد بوالیهدف از این تحقیق بررسی و مقایسه تأثیرات احتمالی دو نوع بازخورد اصلاحی صریح و پلکانی بر استفاده صحیح زبان آموزان ایرانی از حروف معین و نامعین انگلیسی در گفتار شان است. بدین منظور، 45 نفر از دختران زبان آموز سطح متوسط از یک موسسه زبان در شهرستان بهبهان به طور تصادفی به دو Moreهدف از این تحقیق بررسی و مقایسه تأثیرات احتمالی دو نوع بازخورد اصلاحی صریح و پلکانی بر استفاده صحیح زبان آموزان ایرانی از حروف معین و نامعین انگلیسی در گفتار شان است. بدین منظور، 45 نفر از دختران زبان آموز سطح متوسط از یک موسسه زبان در شهرستان بهبهان به طور تصادفی به دو گروه آزمایشی بازخورد اصلاحی صریح و بازخورد اصلاحی داربستی و یک گروه شاهد تقسیم شدند. این تحقیق به روش کمی با طرح شبه آزمایشی در قالب پیش آزمون، جلسات آموزشی، پس آزمون و پس آزمون با تاخیر صورت گرفته است. هر دو گروه آزمایشی در جلسات آموزشی فیدبک متناسب با گروه خود را دریافت کردند در حالی که گروه شاهد هیچ گونه فیدبکی در مورد خطاهایشان دریافت نمیکردند. نتایج تحقیق نشان داد که زبان آموزان گروه بازخورد داربستی و صریح نسبت به گروه شاهد هم در پس آزمون و هم در پس آزمون با تأخیر در استفاده صحیح حروف تعریف معین و نامعین برتر بودند. کاربرد نتایج تحقیق نیز توضیح داده شده است. Manuscript profile -
Open Access Article
23 - بررسی دیدگاههای زبان آموزان در مورد بازخورد اصلاحی صریح و بازخورد اصلاحی داربستی نسبت به استفاده از حروف تعریف انگلیسی در گفتار
Lotfollah Samaee احسان رسایی محمد بوالیاین تحقیق دیدگاههای زبانآموزان ایرانی را در مورد دو نوع بازخورد اصلاحی داربستی و صریح در گفتار بررسی میکند. بدین منظور، 30 نفر زبانآموز دختر سطح متوسط با دامنه سنی 15 تا 20 سال از یک موسسه زبان در شهرستان بهبهان به طور تصادفی به دو گروه بازخورد اصلاحی صریح و با Moreاین تحقیق دیدگاههای زبانآموزان ایرانی را در مورد دو نوع بازخورد اصلاحی داربستی و صریح در گفتار بررسی میکند. بدین منظور، 30 نفر زبانآموز دختر سطح متوسط با دامنه سنی 15 تا 20 سال از یک موسسه زبان در شهرستان بهبهان به طور تصادفی به دو گروه بازخورد اصلاحی صریح و بازخورد اصلاحی داربستی تقسیم شدند. این تحقیق با روش کیفی در قالب مجموعهای از مصاحبههای نیمه ساختار یافته به منظور بررسی عمیق دیدگاه زبانآموزان نسبت به دو نوع بازخورد اصلاحی مورد بحث انجام شد. یافتههای مصاحبه نشان داد که نیمی از زبانآموزان در گروه بازخورد اصلاحی صریح نسبت به این نوع بازخورد نگرشی مثبت داشتند در حالی که نیمی دیگر تمایل چندانی نداشتند. به همین ترتیب، تعداد زیادی از زبانآموزان در هر دو گروه معتقد بودند که بازخورد اصلاحی معلم، حواسشان را پرت میکند. با این حال، اکثریت قریب به اتفاق زبانآموزان در گروه داربستی این نوع بازخورد را مفید دانستند. کاربرد یافتهها نیز توضیح داده شده است. Manuscript profile -
Open Access Article
24 - استفاده از انواع اسکفولد در کلاس های خواندن: موقعیت ها ی تجربی مختلف
Noushin Asadipiran Shahram Afraz Seyyed Ayatollah Razmjooتحقیق ترکیبی حاضر برای اثبات انواع داربست در زبان آموزان زبان انگلیسی تهیه شده است.توانایی خواندن و استراتژی خواندن برای دستیابی به این هدف، یک طرح چند فازی اجرا شد وپژوهشگران در مجموع 80 نفر از دانشجویان مقطع متوسطه دانشگاه آزاد بندرعباس را انتخاب کردنددر ایران. نمونه Moreتحقیق ترکیبی حاضر برای اثبات انواع داربست در زبان آموزان زبان انگلیسی تهیه شده است.توانایی خواندن و استراتژی خواندن برای دستیابی به این هدف، یک طرح چند فازی اجرا شد وپژوهشگران در مجموع 80 نفر از دانشجویان مقطع متوسطه دانشگاه آزاد بندرعباس را انتخاب کردنددر ایران. نمونه به چهار گروه داربست نرم، گروه داربست سخت تقسیم شد..گروه داربست متقابل و گروه داربست مجازی. شرکت کنندگان این گروه ها یک نشستندپیش آزمون درک مطلب، پس آزمون درک مطلب، همراه با آزمون راهبردهای خواندن.علاوه بر این، خود گزارشها و نمونه کارها و مشاهدات و یادداشتهای محققین از طریقبرای تکمیل چک لیست ها استفاده شد. با توجه به رشد توانایی خواندن دانش آموزان، نتایجنشان داد که داربست های پیچیده بالاترین میانگین را در پس آزمون خواندن داشتند. با توجهبا مطالعه راهبردهای مطالعه، یافتهها نشان داد که درمانهای مختلف داربست تأثیراتی بر روی آن دارد.توسعه راهبردهای جهانی، حل مسئله و حمایت از خواندن در میان فراگیران. درگروه داربست سخت بیشترین استفاده را از راهبردهای خواندن داشتند و سپس داربست مجازیگروه گروه های داربست متقابل و نرم به طور مشابه استفاده کمتری از راهبردهای خواندن داشتند. Manuscript profile -
Open Access Article
25 - Investigation of mechanical and biological properties of functionalized polycaprolactone and polyethylene glycol diacrylate based scaffold reinforced with hydroxyapatite
نرجس کوپائی Akbar KarkhanehThe purpose of this study is synthesised of functionalized polycaprolactone and polyethylene glycol diacrylate based scaffold in the presence of hydroxyapatite (HA) particles and investigated of mechanical and biological properties of scaffolds. . At the first step, PCL MoreThe purpose of this study is synthesised of functionalized polycaprolactone and polyethylene glycol diacrylate based scaffold in the presence of hydroxyapatite (HA) particles and investigated of mechanical and biological properties of scaffolds. . At the first step, PCL diol was acrylated with acryloyl chloride and confirmed using fourier transform infrared (FTIR).Then, the scaffolds were synthesized by radical crosslinking reaction of polycaprolacton acrylate and polyethylene glycol diacrylate (PEGDA) in the presence of hydroxyapatite (HA) particles and particulate technique with sodium chloride. The prepared samples were characterized using techniques such as scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), and dynamic mechanical thermal analysis (DMTA). Results show that increasing the ratio of PEGDA to polycaprolactone diacrylate (PCLDA) led to increase of tan δ peak and decrease of compressive modulus of the network, respectively. It was found that the incorporation of HA particles with the polymer matrices resulted in an increased of compressive modulus and a decrease of tan δ peak. Cytocompatability of the scaffolds were assessed by direct contact test and staining cell. Results indicated no toxicity, and cells attached and spread on the pore walls offered by the scaffols. Thus, the results indicated that the PCLDA/PEGDA/HA scaffolds have the potential of being used as promosing substrates in bone tissue engineering. Manuscript profile -
Open Access Article
26 - Preparation and Characterization of Porous Titanium Scaffold Coated with Akermanite
Masoud Arastooei Ali DoostmohammadiThis work aimed at manufacturing and characterizing of titanium scaffolds which is coated with Akermanite for using in bone tissue engineering. In order to creating titanium scaffolds, the primary titanium powder was prepared with spacer particle elements (sodium chlori MoreThis work aimed at manufacturing and characterizing of titanium scaffolds which is coated with Akermanite for using in bone tissue engineering. In order to creating titanium scaffolds, the primary titanium powder was prepared with spacer particle elements (sodium chloride). Akermanite coating was prepared through sol-gel method and applied on the scaffold. The prepared structure was evaluated using scanning electron microscopy (SEM). Coated scaffolds were evaluated after heat treatment by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). In terms of assessing the bioactivity of titanium scaffolds, the samples were immersed in simulated body fluid (SBF). Scanning electron microscopy images of the uncoated and coated titanium scaffolds was prepared after 3,7,14 and 21 days immersing in simulated body fluid (SBF); X-Ray Diffraction analysis (XRD) confirmed the peaks of Akermanite phase in coated samples. According to the results, our study showed that coating of Akermanite on Ti scaffold can increase the bioactivity of titanium scaffolds. The final conclusion of this study confirmed that titanium scaffolds coating with Akermanite will be a suitable and useful choice in bone tissue engineering. Manuscript profile -
Open Access Article
27 - Anisotropy of Mechanical Properties of Poly Lactic Acid Scaffolds Produced by Fused Deposition Modeling for Bone Tissue Engineering
Mohammad Khodaei Reza AminiBiodegradable porous scaffold polymers are good candidates for tissue engineering. In this research, a three-dimensional porous poly-lactic acid (PLA) scaffold was prepared using a Fused Deposition Modeling (FDM) including about 70% porosity. The study of the phases of MoreBiodegradable porous scaffold polymers are good candidates for tissue engineering. In this research, a three-dimensional porous poly-lactic acid (PLA) scaffold was prepared using a Fused Deposition Modeling (FDM) including about 70% porosity. The study of the phases of primary filament and scaffold using the X-ray diffraction (XRD) test shows that no significant phase difference has been created due to the manufacturing process and the polymer retains its phases properties. The results of the mechanical properties evaluation by the compression test show that the mechanical properties of the scaffold are different in both the parallel and perpendicular directions of the Z axis during the printing, and the mechanical properties of the scaffold are of anisotropic property. Microstructural study by Scanning Electron Microscope (SEM) also shows that the morphology of scaffold porosities is different in two directions, and this is the main cause of anisotropic mechanical properties. Anisotropy of the mechanical properties of FDM produced scaffolds should be considered during load bearing applications in vivo. Manuscript profile -
Open Access Article
28 - Comparative Study of the Effects of Strontium and Magnesium Ions on Physical and Chemical Properties of Calcium Phosphate-Gelatin Biomimetic Scaffolds in Bone Tissue Engineering
Amirhossein Moghanian Majid Raz Fathollah MoztarzadehIn this study, biomimetic scaffolds were designed to study the formation of calcium phosphate deposits by using a double diffusion method into gelatin hydrogel in temperature and pH similar to body conditions. Moreover, the effect of magnesium (Mg) and strontium (Sr) io MoreIn this study, biomimetic scaffolds were designed to study the formation of calcium phosphate deposits by using a double diffusion method into gelatin hydrogel in temperature and pH similar to body conditions. Moreover, the effect of magnesium (Mg) and strontium (Sr) ions on properties was investigated. Five different types of specimens with different Sr and Mg ions percentage were synthesized and then porous scaffolds were prepared by freeze-drying method. The scaffolds microstructures were examined by scanning electron microscopy (SEM), which showed a smooth and needle-shaped surface of specimens. Fourier transform infrared spectroscopy (FTIR) results indicated the presence of phosphate and hydroxyl bonds in the structure of the scaffolds, due to the formation of calcium phosphate phases such as HA. The presence of metal ions in the structure caused the displacement of the peaks in X-ray diffraction (XRD) analysis and lattice parameters. Additionally, osteoblast cell culture results also demonstrated M10 and S10 specimens had proper biocompatibility. Additionally, alkaline phosphate (ALP) activity revealed the optimal amount of 10 mol. % Mg and Sr (M10 and S10), which led to the significantly (**p< 0.01, ***p <0.001) growth, proliferation, and differentiation of 292G osteoblasts cells in scaffolds. Moreover, the presence of calcium phosphate improved the biological properties of the specimens. Finally, the results of various analyses confirmed the high capability of the synthesized scaffold as a promising substitute for bone tissue. Manuscript profile -
Open Access Article
29 - On the use of Response Surface Methodology for Optimization of Scaffold Composite of the Hydroxyapatite and Diopside
Nasrin Rafiee Saeid Karbasi amirabas nourbakhsh kamran aminiThe response surface methodology is a statistical approach to design the experiments, modeling and analysis of the effective factors as well as to help optimizing the process. In this study, we use the central composite design technique to select the optimum scaffold co MoreThe response surface methodology is a statistical approach to design the experiments, modeling and analysis of the effective factors as well as to help optimizing the process. In this study, we use the central composite design technique to select the optimum scaffold composite of the Hydroxyapatite and Diopside. This method suggested twenty different scaffold specimens by optimizing the suitable percentage of porosity via determining the percentage weight concentrations of the three effective parameters. After making the scaffold and determining their porosity, the optimum case for composite scaffolds was 77.57 wt% nHA (22.43wt% Di), 0.64wt% lubricant (STPP) and heat treatment temperature 1200. Also, the results of SEM, FTIR, and XRD confirm that the scaffold specimen made with the response method is an ideal specimen for use in bone tissue engineering. In general, according to the results of this research, the response surface methodology can be a useful tool for optimizing composite scaffolds in tissue engineering. Manuscript profile -
Open Access Article
30 - Fabrication and Characterization of Chitosan/ Polycaprolactone Core-Shell Nanofiber Scaffold Containing Platelet-Rich Fibrin by Coaxial Electrospinning Method for Biomedical Applications
AmirAbbas Rastegar Mahboobeh Mahmoodi Mohammad Mirjalili Navid NasirzadehPlatelet-rich fibrin (PRF) is a natural fibrin matrix containing platelets and growth factors in the blood that increases the bone tissue repair. In this study, polycaprolactone/chitosan (scaffold A) and polycaprolactone/chitosan core-shell scaffold containing PRF (scaf MorePlatelet-rich fibrin (PRF) is a natural fibrin matrix containing platelets and growth factors in the blood that increases the bone tissue repair. In this study, polycaprolactone/chitosan (scaffold A) and polycaprolactone/chitosan core-shell scaffold containing PRF (scaffold B) were fabricated by uniaxial electrospinning and coaxial electrospinning methods, respectively and were characterized. Surface morphology, fiber diameter, porosity, mechanical properties, and functional groups on the scaffolds surface were evaluated by scanning electron microscope (SEM) and transition electron microscopy (TEM), displacement liquid method, tensile strength test, and fourier transform infrared (FTIR) spectroscopy, respectively. The average fibers diameter of the scaffold B decreased to 160 nm as compared to 179 nm for the scaffold A. Also, the presence of chitosan containing PRF in the core with the formation of hydrogen bonding with polycaprolactone in the shell of the scaffold B caused a scaffold with excellent mechanical properties and elastic modulus 40 MPa. Cell viability and adherence of bone cells on the surface scaffolds were evaluated via MTT assay. Due to the present of PRF in the scaffold B, the bone cells growth and cells adhesion on the surface of scaffold B increased compared to the scaffold A. Therefore, according to the results of this study, the core-shell scaffold containing PRF can be a good suggestion for use in biomedical applications. Manuscript profile -
Open Access Article
31 - Achillea Santolina Hydroalcoholic Extract Induces Cell Death in Breast Cancer MCF-7 Cells Cultured in Fibrin Gel
Elham Hoveizi Peyman Abdolali nejad It is a continuing effort to develop new anticancer compounds. Some studies have reported that some compounds of Achillea Santolina have inhibitory effects on several cancer cell lines. The present study aims to evaluate the cytotoxic effect of the hydroalcoholic More It is a continuing effort to develop new anticancer compounds. Some studies have reported that some compounds of Achillea Santolina have inhibitory effects on several cancer cell lines. The present study aims to evaluate the cytotoxic effect of the hydroalcoholic extract of Achillea Santolina on MCF-7 human breast cancer cells in fibrin hydrogel. In this experimental study, to prepare the fibrin hydrogel scaffold, an M199 medium containing 10% FBS, 1% penicillin/streptomycin, fibrinogen powder with a concentration of 3 mg/ml, and thrombin with a concentration of 120 u/ml was used. Cultured MCF-7 cells in fibrin gel were treated with hydroalcoholic extract of Achillea Santolina in 25, 50, 100, 250, 500, and 1000 mg/ml concentrations for 24h. Morphology and cellular viability and proliferation were evaluated by DAPI staining and MTT assay at certain days after treatment. Also, the structure of the scaffold and the condition of the cells in the gel were investigated by photographing with a scanning electron microscope.The results of this study showed the cytotoxic effects of Achillea Santolina extract in a dose- and time-dependent manner. The concentration of 500 mg/ml of the extract of A. Santolina was determined as an IC50 concentration. Also, cell viability in the control group and treated group with extract at IC50 concentrations showed significant (P<0.05) differences on days 1, 3, and 5. The hydroalcoholic extract of Achillea Santolina can inhibit MCF-7 cell proliferation and induce cell death in a dose and time-dependent manner. Manuscript profile -
Open Access Article
32 - The Effects of Chitosan/Polyvinilalchol Nanofibers Scaffold on Skin Burns in Adult Male NMRI Mouse
لیلا ریاحی نسیم حیاتی رودباری سهیلا سلحشور کردستانی کاظم پریورTo repair the wounds of burns a wide range of research including the use of bio-scaffolds due to ability to achieve the desired characteristics has been done. According to favorable biological properties of chitosan such as biocompatibility, antimicrobial properties and MoreTo repair the wounds of burns a wide range of research including the use of bio-scaffolds due to ability to achieve the desired characteristics has been done. According to favorable biological properties of chitosan such as biocompatibility, antimicrobial properties and the ability to improve wound healing in biomedical applications, and also due to biocompatibility and lack of stimulation of the immune system of polyvinyl alcohol, the authors decided to investigate the in-vivo effect of Nanofiber scaffolds on the mix of these two polymers on damaged skin.30 NMRI male mice were randomly divided in to a control group and three experimental groups (Scaffolding chitosan: polyvinylalcohol ratio of 0.100, 20.80, 30.70).Under sterile conditions and anesthesia, a 1 cm in diameter and full thickness skin wounds was created on the back of each mouse. Electrospinning nanofiber scaffolds were applied as wound dressing to cover the burn. The burn area was sterile every day and scaffolding was dressing on the wound. After 21 days histological studies have been done on the wound. The results showeda significant meaningful reduction in wound diameter in the experimental group in comparison to control group(p Also increase in the rate of wound shrinkage has been seen in samples containing scaffolds compared to control samples. Manuscript profile -
Open Access Article
33 - The Study Biocompatibility Bone Marrow Stromal Cells by Interactivity Scaffold of Chitosan/Poly Ethylene Oxide
زهره یوزباشی غلامرضا کاکا ویدا حجتی عبدالحسین شیرویSeveral studies have been performed to achieve a BioCompatibility scaffold with stem cells. The aim of this study was to present biodegradable scaffold of chitosan (Cs)/poly ethylene oxide (PEO) and studies to evaluate the ability of growing, proliferation, undifferetat MoreSeveral studies have been performed to achieve a BioCompatibility scaffold with stem cells. The aim of this study was to present biodegradable scaffold of chitosan (Cs)/poly ethylene oxide (PEO) and studies to evaluate the ability of growing, proliferation, undifferetation and cell death of bone marrow stromal cells (BMSCs) on them. At first, Cs/PEO scaffold made in 3, 1 ratio. This scaffold lies on 1% gelatin at 24 plates and then sterilized. BMSCs extract from adult rats femur and after three passages were implated in the empty house plate as control BMSCs and also on scaffold. The rate of cell proliferation, undifferentiation, and cell death were studied at two, four and six days after 3rd passage in plate. The results showed that the morphology of cells maintained on Cs/PEO scaffold and was simiar to control group. Studies showed that the percentage of differentiation of BMSCs and cell death on scaffold of six day was similar to control. Proliferation, undifferentiation and cell death of BMSCs on biodegradable Cs/PEO scaffold result a model of scaffold that can be used in tissue engineering and cell therapy. Manuscript profile -
Open Access Article
34 - Differentiation Effect of Exosomes Derived from Monocytes and Macrophages on Chitosan Alginate Drip Scaffolds
Nasrin Hosseini Javad Baharara Khadijeh Nejad Shahrokhabadi Saeedeh Zafar BalanezhadBone injuries are one of the challenges of medical science which costs a lot of money in the world for the treatment every year. Exosomes are nanovesicles which carry the proteins and genetic material into the target cell, which in turn, induces the proliferation, cell MoreBone injuries are one of the challenges of medical science which costs a lot of money in the world for the treatment every year. Exosomes are nanovesicles which carry the proteins and genetic material into the target cell, which in turn, induces the proliferation, cell survival, and differentiation in the recipient cell. These features make exosomes a proper differentiation factor. This study aimed to investigate the differentiating effect of exosomes derived from the monocytes and macrophages on chitosan alginate drip scaffolds. Chitosan-alginate scaffolds were made by drip method with fat stem cells. Monocytes were cultured, and the supernatant was collected, and the exosomes were collected by the ultracentrifugation. Exosomes were identified by DLS and SEM methods. The samples were treated, and then MTT and DAPI tests were performed. The osteogenic differentiation was examined by alkaline phosphatase and real-time PCR. MTT results showed that the survival rate of differentiated cells with exosomes on days 7, 14, and 21 was increased compared to control group. Moreover, DAPI results showed no cell death in the treatment groups, and the results of the alkaline phosphatase test showed an increase with the control group, and also the results of real-time PCR increased the expression of genes BMP2/6, SMAD4 and differentiation genes Osc and Opn in the treatment group. This study showed that the exosomes derived from the monocytes and macrophages could cause survival and osteogenic differentiation of the fat mesenchymal cells in the chitosan alginate scaffold. Manuscript profile -
Open Access Article
35 - Study of Biological, compound and Surface Topography if Granule Scaffold Hydroxyapatite/Ostrich Eggshell for Bone Tissue Engineering
Farideh Alahgahi Esmail Fattahi Mohsen Saeedi Mir Mahmoud Mortazavi RoudmianeThis study aimed to investigate the topography surface and ability of this scaffold in combination with the mesenchymal and fibroblast cells. In this regard, several different ratios of hydroxyapatite / ostrich eggshell scaffold were created to determine the surface pro MoreThis study aimed to investigate the topography surface and ability of this scaffold in combination with the mesenchymal and fibroblast cells. In this regard, several different ratios of hydroxyapatite / ostrich eggshell scaffold were created to determine the surface properties and responsiveness to the cells with different types of hydroxyapatite/ostrich eggshell (OsE/HA) combinations (ratios 0-1, 1-2, 1-1, 2-1, 1-0). Natural biomaterials including ostrich eggshells which contain the minerals such as calcium carbonate, play a major role in the fabrication of hard tissue scaffolds. Surface characterization was investigated using SEM and recognizing the scaffold properties by XRD, FTIR. Finally, direct contact (toxicity) test with fibroblast-like cells (L929) was performed to study the cytotoxicity response. After the scaffold toxicity test, the material was ready for in vitro acceptance test, which mesenchymal stem cells isolated from the adipose tissue of New Zealand white rabbit (RADMSCs) which kept with this bioscaffold. This study's results showed that this scaffold has the best biocompatibility and the highest surface lacuna for cell adsorption and attachment with hydroxyapatite / ostrich eggshell. Bioscaffold OsE/HA with the ratio of 1-2 has the best cell attachment among other groups. Natural biomaterials with synthetic materials can promote the bone tissue engineering in many ways. Manuscript profile -
Open Access Article
36 - Investigating the Behavior of MG63 Cells on Polycaprolactone and Polycaprolactone/Collagen 3D Scaffolds for Bone Regeneration
Zahra Ebrahimi Shiva Irani Abdolreza Ardeshirylajimi Ehsan Seyedjafari3D printing of scaffolds at low temperatures is very promising for making artificial bone graft alternatives with more performance than traditional techniques. One of the most promising strategies in bone tissue engineering has focused on the development of biomimetic s More3D printing of scaffolds at low temperatures is very promising for making artificial bone graft alternatives with more performance than traditional techniques. One of the most promising strategies in bone tissue engineering has focused on the development of biomimetic scaffolds. Ceramic-based scaffolds with osteogenic ability and mechanical properties are promising candidates for bone repair. The aim of this study was to adapt the flexibility and increasing the effect of inducing osteogenesis of Polycaprolactone (PCL) scaffold prepared by fused deposition modeling (FDM) method, using the combination of Collagen (COL) as a natural polymer with synthetic polymer and to investigate the behavior of MG63 cells on it. on it. After preparing the scaffold, scanning electron microscope (SEM), energy dispersive X-ray (EDX) and ATR-FTIR spectroscopy were used. After 1,7,14 days, the ossification process of MG63 cells in different treatments was performed using alizarin red staining and alkaline phosphatase activity. The non-toxicity of scaffolds was also evaluated by MTT assay to ensure cell proliferation. From under a microscope, it was found that tissue engineering scaffolds distributed and connected almost rectangular pores evenly. The PCL/COL scaffold showed a significant difference in terms of viability compared to the polycaprolactone scaffold only in the differential medium (P ≤ 0.0001). The results of evaluating ALP activity in PCL/COL scaffolds were significantly higher than uncoated PCL scaffolds and control (P ≤ 0.0001). The results of this study showed that the use of PCL/COL scaffold can be considered a suitable medium for proliferation and differentiation of MG63 cells in bone tissue engineering. Therefore, the PCL/COL composite scaffold prepared by FDM printer can be widely used in bone tissue engineering due to the cell survival by COL. Manuscript profile -
Open Access Article
37 - Fabrication and Evaluation of Morphological, Structural and Biocompatibility Properties of Chitosan Nanofibrous Scaffolds Coated with ZIF-8 Metal-organic Framework for Application in Wound Dressing
Mahdi HasanzadehIn this study, chitosan-based nanofibrous scaffold was produced for application in wound dressing by electrospinning method. Then, the zinc-based metal-organic framework (ZIF-8) was in situ synthesized method on nanofibrous scaffold. The effect of growth time of ZIF-8 c MoreIn this study, chitosan-based nanofibrous scaffold was produced for application in wound dressing by electrospinning method. Then, the zinc-based metal-organic framework (ZIF-8) was in situ synthesized method on nanofibrous scaffold. The effect of growth time of ZIF-8 crystals on chitosan nanofibrous scaffolds was studied in order to achieve a uniform and continuous coating. The morphology and structure of the produced scaffolds were evaluated using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). In order to evaluate the potential of fabricated scaffolds in wound dressing, surface wettability, antibacterial activity, biocompatibility and cell viability were also investigated. The electron microscope images showed that after 30 minutes, ZIF-8 nanoparticles grew with a relatively uniform distribution with an average size of 80 nm on the chitosan scaffold. The results showed that the scaffolds were biocompatible after 24 hours of cell culture and also had antibacterial properties against Gram-negative and Gram-positive Escherichia coli and Staphylococcus aureus, respectively. Due to the appropriate structural and morphological properties as well as the appropriate biocompatibility and antibacterial performance of the nanofibrous scaffold coated with a metal-organic framework, it can be used as a suitable candidate in wound dressing applications. Manuscript profile
Sanad
Sanad is a platform for managing Azad University publications
Links
Related Centers
Technical Support
Official pages
The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024