Achillea Santolina Hydroalcoholic Extract Induces Cell Death in Breast Cancer MCF-7 Cells Cultured in Fibrin Gel

Subject Areas : Journal of Animal Biology

Elham Hoveizi ¹ , Peyman Abdolali nejad ²

1 - Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 - Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Received: 2022-07-26 Accepted : 2022-09-24 Published : 2023-05-22

Keywords: breast cancer, Achillea Santolina, cell viability, Hydrogel Scaffold,

Abstract :

 It is a continuing effort to develop new anticancer compounds. Some studies have reported that some compounds of Achillea Santolina have inhibitory effects on several cancer cell lines. The present study aims to evaluate the cytotoxic effect of the hydroalcoholic extract of Achillea Santolina on MCF-7 human breast cancer cells in fibrin hydrogel. In this experimental study, to prepare the fibrin hydrogel scaffold, an M199 medium containing 10% FBS, 1% penicillin/streptomycin, fibrinogen powder with a concentration of 3 mg/ml, and thrombin with a concentration of 120 u/ml was used. Cultured MCF-7 cells in fibrin gel were treated with hydroalcoholic extract of Achillea Santolina in 25, 50, 100, 250, 500, and 1000 mg/ml concentrations for 24h. Morphology and cellular viability and proliferation were evaluated by DAPI staining and MTT assay at certain days after treatment. Also, the structure of the scaffold and the condition of the cells in the gel were investigated by photographing with a scanning electron microscope.The results of this study showed the cytotoxic effects of Achillea Santolina extract in a dose- and time-dependent manner. The concentration of 500 mg/ml of the extract of A. Santolina was determined as an IC50 concentration. Also, cell viability in the control group and treated group with extract at IC50 concentrations showed significant (P<0.05) differences on days 1, 3, and 5. The hydroalcoholic extract of Achillea Santolina can inhibit MCF-7 cell proliferation and induce cell death in a dose and time-dependent manner.

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