The Effect ofCyanobacteriaNostoc . Sp ISC 113Polysaccharide on the Proliferation and Adhesion of Endothelial Cells to Repair the Vessel
Subject Areas :ome foroh maleki 1 , Mahrouz Dezfulian 2
1 - کارشناس ارشد گروه میکروبیولوژی، دانشکده علوم، واحد کرج، دانشگاه آزاد اسلامی، البرز. ایران.
2 - استادیار گروه میکروبیولوژی، دانشکده علوم، واحد کرج، دانشگاه آزاد اسلامی، البرز. ایران.
Keywords: Endothelial, Fibroblast, Polysaccharide,
Abstract :
Inroduction & ObjectiveThe applications of extracellular materials of marine organisms are widely expanded. Some of the secreted materials of cyanobacteria are polysaccharides. In the present study extracted polysaccharide of Nostoc. sp ISC 113 cyanobacteria evaluated as cellular scaffolding on endothelial cellsMaterials and Methods:The polysaccharide extracted from cyanobacteria Nostoc.sp ISC 113. The cell scaffold made from polysaccharide with collagen and hyaluronic acid and its effects on the growth and proliferation assessed by MTT assay and the cell adhesion of HUVEC endothelial to scaffold evaluated, by crystal violet staining and microscopic studies. The colony assay performed for clonogenic endothelial cells.ResultsThe data obtained from MTT assay showed that the extracted polysaccharide of Nostoc cyanobacteria increased the proloiferation, adhesion, and clonogenic endothelial HUVEC cells.Conclusion: The extracted polysaccharide of Nostoc is appropriate natural polymer to build scaffold or repair the capillaries
