Fabrication of electrospun silk fibroin scaffold and the effect of its pre-incubation in culture medium on survival and adhesion of rat bone marrow mesenchymal cells

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Maryam Janitermi ¹ , esmail fattahi ² , Seyed Gholam Ali Jorsarai ³

1 - Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2 - Department of biology, Ayatollah Amoli branch,Islamic Azad University
3 - Fatemeh Zahra Infertility and Reproductive Health Research Centre, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

Received: 2021-03-14 Accepted : 2021-07-15 Published : 2021-05-22

Keywords: scaffold, Electrospinning, Cell Culture, Fibroin,

Abstract :

Objective: This study was performed to synthesize electrospun silk fibroin scaffold and to investigate the time-dependent effect of its pre-incubation in culture medium on the adhesion and proliferation of cells seeded on the scaffold. Methods: sericin was removed from silk cocoon and fibrin solution (3% w/v) was prepared using formic acid. Then, electrospun silk fibroin scaffold was made using lab-scale electrospinning machine and evaluated by scanning electron microscopy. Pre-incubation of scaffolds in culture medium was performed for 0, 1, 6, and 10 days and the hydrophilicity of scaffolds was evaluated by measuring the water contact angle. Rat bone marrow mesenchymal cells were then isolated, cultured, and seeded on scaffolds. After 21 days of cell seeding, cell viability (MTT method) and genomic DNA concentration of cells attached to the scaffold were evaluated. Results: The results showed that increasing the pre-incubation time in the culture medium decreased the water contact angle and increased the survival and proliferation of cells. In general, the present study showed that pre-incubation of electrospun fibroin scaffold with constant elasticity in culture medium leads to increased scaffold hydrophilicity and consequently increases the proliferation and survival of mesenchymal cells seeded on it. Conclusion: these findings can be used as an effective factor in seeding cartilage cell on scaffolds in tissue engineering.