Evaluation of adhession and viability of endometrial stem cells-derived osteoblast-like cells cultured on PLGA/HA scaffold

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

mojdeh salehi ¹ , jafar ai ² , nasrin lotfibakhshaiesh ³ , shiva irani ⁴ , سمیه ابراهیمی باروق ⁵

1 - Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2 - Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
3 - Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
4 - Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
5 - دانشکده فنآوریهای نوین پزشکی، دانشگاه علوم پزشکی تهران

Received: 2018-06-11 Accepted : 2018-09-22 Published : 2019-02-20

Keywords: Tissue Engineering, endometrial stem cells, electrospining, PLGA/HA scaffold, bone differentiation,

Abstract :

Introduction: The engineered tissue structure includes three components: cells, signaling pathway and scaffold. The polylactide-co-glycolide/hydroxyapatite (PLGA/HA) has attracted much attention due to their optimal properties because of using PLGA polymer and hydroxyapatite. The aim of this study was to evaluate the biocompatibility effect of PLGA/HA scafffold on osteoblast differentiated from hEnSCs. We aimed to evallute the adhesin of cells on scaffolds. hEnSCs were isolated from human endometrium tissue and induced the osteogenic differentiation, then the differentiated cells were cultured on PLGA/HA synthetic scaffolds. A nanocomposite scaffolds based on PLGA/HA were fabricated by electrospinning methode and behavior of differentiated osteoblast cells was evaluated after seeding cells on this scaffold. Osteogenesis was investigated in terms of attachment, alkaline phosphatase activity and gene expression. Morphogenic of cells in scaffold was evaluated by SEM and biocampatibility of this scaffold was confirmed by MTT assay. The alkaline phosphatase activity confirmed osteoblast differentiation. SEM studies showed that the surface properties of scaffold were desirable and the cells had the ability to attach and proliferate better on the nanocomposite scaffolds. It has been shown that the nanocomposite scaffolds have appropriate properties to support the attachment of differentiated osteoblast cells.