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      • Open Access Article

        1 - The co-assessment of antioxidant capacity and flavonoids levels of alcoholic extract of some of current traditional medicine plants
        فاطمه کریم نژاد فهیمه فیروزبخت صدیقه میرلطیفی شهرزاد قدیمی فاطمه فرجادمند
        Background: In the last years, there has been growing interest in finding plants for treatment andprevention of disease. Borage officinalis, Camellia sinensis, Chamaemelum nobile, Morus albaand Rosmarinus officinals are usually used in traditional medicine. The aim of t More
        Background: In the last years, there has been growing interest in finding plants for treatment andprevention of disease. Borage officinalis, Camellia sinensis, Chamaemelum nobile, Morus albaand Rosmarinus officinals are usually used in traditional medicine. The aim of this experimentalstudy was co assay of antioxidant and flavonoids levels of alcoholic extraction of these plants. Inthis study the antioxidant activity was surveyed by applying iron ion reducing assay (FRAPassay) and flavonoids levels with measuring the complex flavonoids with trivalent aluminumion. Results from the present study show,the alcoholic extraction of Rosmarinus officinals havea marked antioxidant activity, and the high levels of flavonoids was recorded by Chamaemelumnobile. Camellia sinensis had both effects; antioxidant activity, flavonoids levels. The using ofscreening tests such as the mentioned methods are useful for finding biological components withdifferent effects Manuscript profile
      • Open Access Article

        2 - Evaluation of the Antioxidant Activity of Some Rosaceae Plants as an Alternative to the Synthetic Antioxidants in Food Industry
        فائزه Kamali A.R Sadeghi Mahonak زهرا Nasiri far
        Introduction : The applications of natural antioxidants with medicinal values are growing in the preventive medicine and food industries. This study investigates the antioxidant ability of five species of Rosaceae family and compares the activities with the synthe More
        Introduction : The applications of natural antioxidants with medicinal values are growing in the preventive medicine and food industries. This study investigates the antioxidant ability of five species of Rosaceae family and compares the activities with the synthetic antioxidants BHT, BHA as well as the natural ascorbic acid.   Materials and Methods : In this study, the phenolic content of the methanolic extract of the samples were isolated and the antioxidant activities of five species of Rosaceae were determined by using DPPH free radical and nitric oxide scavenging , and the results were compared to the synthetic antioxidant Results : The results showed that the content of studied plant samples ranged from 0.51±0.41 – 35.53±0.89 mgGAEg -1. Concentraition of extract for scavening 50 percent of free radicals and nitric oxide radicals varied from 77.42 ± 0.91 to 1484.2 ± 96.44 and 233.24±3.39- 1572.46±203.76 Ug/ml. The highest phenolic amount was related to the Rosa damascena Mill leaf and next grade Cydonia oblonga Mill leaf. Extract of damask Rose, Quince leaves, damask Rose petals, Quine fruit and seed and Almond seed had high antioxidant activity and no significant difference was observed between them and the synthetic antioxidants (BHT, BHA and Vit C). Conclusion : The results of this study clearly suggested that there is a significant relationship between the antioxidant activity and the phenolic concentration and the extracts of damask Rose, Quince leaves, damask Rose petals, Quince fruit and seed and almond seed might be employed as appropriate alternatives to the synthetic antioxidants. Manuscript profile
      • Open Access Article

        3 - The Impact of Short-Term Garlic Supplements on Serum Total Antioxidant Capacity and Malondialdehyde in Active Men after Eccentric Exercise
        A. Sadeghi M. Gholami
        Introduction: Garlic increases the antioxidant levels of the body. The antioxidant defensive system in the body can reduce the oxidative stress and reduces the damage to the cells. The aim of this study is to investigate the effects of short-term supplementation of garl More
        Introduction: Garlic increases the antioxidant levels of the body. The antioxidant defensive system in the body can reduce the oxidative stress and reduces the damage to the cells. The aim of this study is to investigate the effects of short-term supplementation of garlic on the total anti-oxidative capacity, malondialdehyde serum in active men after eccentric exercise. Materials and Methods: Twenty healthy athlete males (age of 24 ± 3 years, body mass index of 24.4±1.3 kg.m-2, and VO2 max of 49.1±3.2 ml/kg/min) were randomly assigned to two equal groups: experimental (daily intake of 700 mg garlic capsules) and control (placebo) groups who used dextrose capsules for four weeks. The blood samples were taken at baseline, after completing a four-week period supplementation and after eccentric exercise (45 minutes of running with 9 set of 5-minutes with 80% oxygen consumption and 10% with a negative slope between each set of 2 minute active recovery with zero slopes). The parameters were analyzed using repeated measure ANOVA, Bonferroni, with a significance level of a=0.05. Results: Garlic supplementation for four weeks increased the total antioxidant capacity in the pre exercise (P<0.05) significantly, but decreased after downhill running in two groups (P <0.05). Garlic supplementation did not have any effects on Malondialdehyde, therefore, there are not any significant differences between Malondialdehyde after supplementation among the two groups, but increased after downhill running in two groups. Conclusion: Garlic supplementation might reduce oxidative stress damage by increasing of TAC after eccentric exercise in active men.   Manuscript profile
      • Open Access Article

        4 - The Effect of Aerobic Training and Octopamine on Antioxidant Markers and Adiponectin in Rats Fed by Deep Heated Oils
        A. Taavonkerdar Z. Etemad K. Mohamadzadeh Salamat K. Azizbeigi
        Introduction: Today, deep frying is a common cooking method in which fat is used as a heattransfer medium, during which foods with unique properties in terms of taste, texture andappearance are produced. Deep heated oils produce toxins that endanger people's health. The More
        Introduction: Today, deep frying is a common cooking method in which fat is used as a heattransfer medium, during which foods with unique properties in terms of taste, texture andappearance are produced. Deep heated oils produce toxins that endanger people's health. Thepurpose of the present study was to investigate the effect of aerobic training and Octopamineon some antioxidants indices and Adiponectin in rats fed with deeply heated oils.Materials and Methods: 40 Wistar rats were selected and randomly divided into controlpoisoning(CP; n=8), Training-Poisoning (TP; n=8), Supplement-Poisoning (SP; n= 8),Supplement-Training-Toxicity (STT; n=8) and healthy-control group (HC; n=8) wereincluded, and they were fed for four weeks. The training program lasted for four weeks withan average intensity of 50-65% vo2max daily for 20 minutes. Octopamine was used as asupplement for 4 weeks and 5 days a week using itraperitoneal injection of 81 μmol / kg.Super oxide dismutase (SOD) activity, Total Antioxidant Capacity (TAC), and Adiponectin(ADIPOQ) concentration were measured in the plasma.Results: The results showed that the SOD activity, TAC, and ADIPOQ concentration wereincreased in the TP, STT, and SP than CP (p≥0.001). However, there is no significantdifference between TP, STT, and SP in the mentioned variables (p=0.05).Conclusion: It might be concluded that exercise training or taking octopamine alone to havethe health benefits of both, and in terms of cost, each seems to be more cost-effective thancombining both. Manuscript profile
      • Open Access Article

        5 - Correlation of Total Antioxidants Levels and Malondialdehyde with Sperm Parameters and Chromatin integrity in Asthenoteratozoospermia Men
        rahil jannatifar Zahra Ebrahimi Hamid piroozmanesh seyedeh Saeideh Sahraei
        Background and purpose: Sperm dysfunction caused by reactive oxygen species (ROSs) is one of the major causes of infertility in men, which leads to, lipid peroxidation (LPO) and the formation of stable peroxidation products like Malondialdehyde (MDA) in seminal plasma. More
        Background and purpose: Sperm dysfunction caused by reactive oxygen species (ROSs) is one of the major causes of infertility in men, which leads to, lipid peroxidation (LPO) and the formation of stable peroxidation products like Malondialdehyde (MDA) in seminal plasma. MDA is effective factor in reducing fertility. The aim of this study is to determine two biochemical markers of oxidative stress; TAC and MDA, and correlation to sperm parameters and chromatin integrity at asthenoteratozoospermia men. Materials and methods: In this study, a case-control study was carried out in two groups of 50 fertilized men and 50 asthenoteratozoospermia men in Qom IVF center; Iran. Semen analysis was performed according to WHO (2010) guidelines. DNA damage, protamine deficiency and TAC, MDA levels in all patients were measured by kit methods, respectively. Results: According to our results, the percentage of sperm parameters in the infertile men were lower than the fertile men (p <0.05). Also, there was a significant difference in DNA damage and protamine deficiency between the two groups (p <0.05). Lower TAC levels (1.82± 0.11 vs. 2.25 ± 0.13) and higher MDA levels (2.36 ± 0.09 vs. 1.97 ± 0.10) were observed in infertile men with asthenoteratozoospermia compared to fertile men.Conclusion: These results suggest that decreasing TAC and increasing MDA lead to low sperm parameters and high DNA integrity in sperm of asthenoteratozoospermia men. Therefore, evaluation of oxidative status and antioxidant defenses system may be as a useful tool for diagnosis and treatment of male infertility especially in idiopathic male infertility Manuscript profile
      • Open Access Article

        6 - The response of oxidative stress indicators to grape seed extract supplementation and resistance training in bodybuilders
        fariba joshaghani hamzeh rahmani amin yosefvand
        Black grape seed extract (GSE) is one of the supplements that have strong antioxidant effects with flavonoid properties that can prevent or reduce cell damage. The purpose of this study was to investigate the response of oxidative stress indicators to grape seed extract More
        Black grape seed extract (GSE) is one of the supplements that have strong antioxidant effects with flavonoid properties that can prevent or reduce cell damage. The purpose of this study was to investigate the response of oxidative stress indicators to grape seed extract supplementation and resistance training in bodybuilders. The methodology of the present research was conducted in the form of a semi-experimental design in two supplement and control groups with blood sampling on two occasions before and after taking the supplement in a double-blind manner. The statistical population of the present study consisted of 24 male bodybuilders with an age range of 15-19 years and they were divided into two groups: supplement and placebo. About 5 ml of blood was taken from the subjects in each of the fasting blood sampling sessions. Measurement of blood indices included antioxidant capacity (TAC), malondialdehyde (MDA), creatine kinase (CK), and lactate dehydrogenase (LDH). The normality of data distribution was done using ShapiroWilk tests. The findings of the research results indicate that performing bodybuilding resistance exercises caused a significant increase in cell damage indices (creatine kinase and lactate dehydrogenase) and malondialdehyde index and a significant decrease in total antioxidant capacity (P<0.05). Consumption of grape seed extract increased total antioxidant capacity. The present results show that performing bodybuilding activities increases the indicators of cell damage and oxidative stress, and the consumption of grape seed extract (100 mg per day) increases the total antioxidant capacity in male bodybuilders. Manuscript profile
      • Open Access Article

        7 - Effect of freeze-drying and spray-drying on total phenolics content and antioxidant activity from aqueous extract of Justicia secunda leaves
        Emmanuel N’dri Koffi Amian Brise Benjamin Kassi Felix Anoh Adje Yves François Lozano Yves-Alain Bekro
        Manuscript profile
      • Open Access Article

        8 - Effect Of Exhaustive Exercise and Supplement N-Acetyl Cysteine on TAC, MDA and CRP in Wistar Rats after a Single bout Of Exhaustive Exercise
        Ali Aalizadeh Najmeh Mobseri
        Inroduction and ObjectivePhysical activity exhausting the release of reactive oxygen species (ROS) and nitrogen species activated (RNS) is a well has a dual role as both deleterious and beneficial species is known. N - Acetylcysteine (NAC) acetylating of the amino-L-cys More
        Inroduction and ObjectivePhysical activity exhausting the release of reactive oxygen species (ROS) and nitrogen species activated (RNS) is a well has a dual role as both deleterious and beneficial species is known. N - Acetylcysteine (NAC) acetylating of the amino-L-cysteine, which is converted in the body into metabolites that are capable of stimulating glutathione synthesis may be resulting in the elimination of free radicals. Effective properties of complementary N – acetylcysteine ​reduces fatigue and effects on sub maximal contractions.Material and Methods: This study used 16 female Wistar rats weighing 150 to 200 g and (8 to 10 weeks old) were divided into 2 groups. First group effervescent tablets were given (600 mg) of N-acetyl cysteine dissolved in water) four hours before the experiment by gavages and group was taken as the control. Blood samples were taken 1 hour before starting the exhaustive exercise - immediately after the exhaustive exercise and after one hour at rest.Results: Significant decrease was observed in the concentration of MDA immediately after the exhaustive exercise in the group consumed NAC and blood concentration of TAC one hour after exhaustive exercise as compared with the control (P≤0.05). Reactive protein concentrations - C (CRP) in the period before, immediately and after exhaustive exercise did not show any significant difference.Conclusion:The results of our study demonstrated that orall administration of N-acetyl cystein at least four hours before an exhaustive exercise can significantly reduce the harmful effects of oxidative stress in rats. Manuscript profile
      • Open Access Article

        9 - Evaluation of physiological and biochemical changes of strawberry cv. Gaviota in response to Mycorrhizal and Trichoderma fungi under selenium treatment
        Ali Lachinani Seyed jalal Tabatabaei Amir Bostani Vahid Abdossi Saeed Rezaee
        10.30495/qafj.2023.1985550.1071
        This study evaluated the effects of Mycorrhiza (Rhizophagus irregularis), Trichoderma (Trichoderma harzianum) and selenium on some physiological traits of Gaviota strawberry as a factorial experiment based on a completely randomized design with three replications in the More
        This study evaluated the effects of Mycorrhiza (Rhizophagus irregularis), Trichoderma (Trichoderma harzianum) and selenium on some physiological traits of Gaviota strawberry as a factorial experiment based on a completely randomized design with three replications in the Shahed University, Faculty of Agricultural Sciences research greenhouse in 2017. The treatments included fungi (No inoculation, Mycorrhiza, Trichoderma) and selenium (0, 0.5, 1, 2 and 4 mg/kg soil). The results showed that the use of symbiotic fungi, especially Trichoderma, could significantly increase the pH, total soluble solids (TSS), antioxidant capacity and anthocyanin of the fruit, as well as increase the amount of protein, chlorophyll and carotenoid of Gaviota strawberry leaves. Furthermore, selenium treatment, especially at low concentrations (0.5 and 1 mg/kg soil), could increase the antioxidant and anthocyanin capacity of the fruit, as well as the amount of protein and chlorophyll in the leaves. Overall, it is possible to recommend the using of symbiotic fungi, especially Trichoderma, along with low concentrations of selenium (0.5 and 1 mg/kg soil) in Gaviota strawberry cultivation programs. Manuscript profile
      • Open Access Article

        10 - Effect of chitosan and salicylic acid along with cottage cheese and aloe vera extracts on qualitative and quantitative indicators of mandarin fruit (Citrus reticulate) cv. Unshiu
        Amir Mokhtarpoor bazmin abadi Abdol Rasool Zakerin behnam behroznam Abolhossein Aboutalebi Jahromi Sayed Abdolhossein Mohammadi Jahromi
        10.30495/sarj.2023.1999824.1162
        To investigate the effect of chitosan, salicylic acid, aloe vera gel and mallow extract treatments on the qualitative factors of mandarin and to determine their best concentration, a factorial experiment was conducted in a completely randomized design at the Islamic Aza More
        To investigate the effect of chitosan, salicylic acid, aloe vera gel and mallow extract treatments on the qualitative factors of mandarin and to determine their best concentration, a factorial experiment was conducted in a completely randomized design at the Islamic Azad University, Science and Research Branch. Experimental treatments included chitosan (0.5, 1 and 1.5%), salicylic acid (1, 1.5 and 2 mM), aloe vera gel (15, 30 and 45%) and mallow extract (15, 30 and 45%) ) were immersed and then stored for 45 days (storage period with 4 levels 0, 15, 30 and 45 days). Fruits juice pH, titratable acid, total soluble solids, fruit weight loss, carotenoids and ascorbic acid content and antioxidant capacity were measured. The results showed that during storage, the pH, total soluble solids, weight loss and carotenoids content increased and the amount of titratable acid, ascorbic acid and antioxidant capacity decreased. The most effective treatment in maintaining pH, titratable acid, soluble solids, carotenoid content, ascorbic acid and antioxidant capacity was salicylic acid 2mM, which is recommended for use after harvesting. Manuscript profile
      • Open Access Article

        11 - Determination of total antioxidant capacity with nanoparticles
        Safieh Momeni
        Total antioxidant capacity (TAC) as the cumulative activity of antioxidants in a sample is an important parameter in the analysis of biological or food matrices. Therefore, it is very important to evaluate the total antioxidant capacity of the substances in the diet and More
        Total antioxidant capacity (TAC) as the cumulative activity of antioxidants in a sample is an important parameter in the analysis of biological or food matrices. Therefore, it is very important to evaluate the total antioxidant capacity of the substances in the diet and biological fluids. Based on this, many methods check their antioxidant capacity and effectiveness in different conditions. However, there is often no strong correlation between the capacities measured on the same materials with different methods, which is due to the variety of active materials, mechanisms and different characteristics such as different types of antioxidants, the presence of other interfering substances in the sample, lack of participation of antioxidants are used in the method reaction. In recent years, different analytical methods based on nanoparticles have been developed to determine the antioxidant capacity of foods and plant materials.In these measurement methods, nanoparticles such as gold, silver, iron oxide, manganese oxide, quantum dots and cerium oxide have been used. In this article, we review some of the researches conducted in the field of total antioxidant capacity measurement. Manuscript profile
      • Open Access Article

        12 - The effect of zinc-methionine supplementation on antioxidant status and expression of interleukin-4 and interleukin-6 genes in female rats under heat stress
        Matin Jamei Ali Asghar Sadeghi Mohammad Chamani
        10.30495/zisti.2023.1973380.1144
        Introduction:During heat stress, the animal body requires more antioxidant compounds and activity of antioxidant enzymes. Zinc plays a role in the structure and activity of antioxidant enzymes. One of the ways to supply the zinc requirement, is to use organic zinc suppl More
        Introduction:During heat stress, the animal body requires more antioxidant compounds and activity of antioxidant enzymes. Zinc plays a role in the structure and activity of antioxidant enzymes. One of the ways to supply the zinc requirement, is to use organic zinc supplements, which zinc combined with methionine, and have more intestinal absorption Aim: This study was done to evaluate the effects of different doses of zinc-methionine supplementation on the blood antioxidant status and the expression of interleukin 4 and 6 genes in rats exposed to heat stress.Materials and methods: In a completely random design, 20 female rats were divided into four treatment groups with five replicates. Rats were kept at a temperature of 31±2 °C for 20 hours and at a temperature of 38±2 °C for 4 hours per day (to create heat stress). The rats in the control group were fed standard pellets without additives, and the three experimental groups were fed standard pellets plus 15, 30 and 45 mg zinc-methionine supplement per kilogram of dry matter for 30 days. At the end of the experiment, a blood sample was collected from the inferior vena cava. Total antioxidant capacity, malondialdehyde concentration, antioxidant enzymes, liver enzymes in the serum and the expression of interleukin-4 and interleukin-6 genes were measured.Results: Serum zinc concentration increased linearly with increasing zinc dose in the diet (P<0.05). The serum activity of two enzymes, alanine aminotransferase and aspartate aminotransferase decreased linearly with increasing zinc-methionine dose in the diet (P<0.01). The highest activity of glutathione reductase and glutathione peroxidase was observed in the group receiving 30 mg/kg, and the lowest activity of these enzymes was observed in the control group. In general, the relative expression of the interleukin-4 gene was increased, and the relative expression of the interleukin-6 gene was decreased (P<0.05). The highest expression of interleukin-4 gene belonged to the group receiving 30 mg, and the highest relative expression of interleukin-6 gene belonged to the control group and the group receiving 15 mg/kg.Conclusion: The results of this study showed that the dose of up to 30 mg of zinc-methionine per kg of diet used in this study increases the activity of superoxide dismutase enzyme and glutathione peroxidase enzyme activity, decreases the activity of liver enzymes in the serum, increases the expression of the anti-inflammatory gene (Interleukin-4) and decreases the expression of inflammatory gene (Interleukin-6), which indicates the reduction of oxidative stress and the reduction of the effects of heat stress in the body of the rat. Manuscript profile
      • Open Access Article

        13 - The effects of piperine supplementation on colony population, body weight after emerging, viability and vitellogenin gene expression in honey bees under heat stress
        Zahra Farhadi Ali Asghar Sadeghi Farahnaz Motamedi Sedeh Mohammad Chamani
        10.30495/zisti.2023.1968199.1138
        Aim: This study was done to evaluate the effects of piperine (phytogenic material in pepper) supplementation on antioxidant capacity, vitellogenin gene expression, the viability and body weight of honey bees at the time of emerging.Materials and methods: 32 bee hives we More
        Aim: This study was done to evaluate the effects of piperine (phytogenic material in pepper) supplementation on antioxidant capacity, vitellogenin gene expression, the viability and body weight of honey bees at the time of emerging.Materials and methods: 32 bee hives were divided to 4 treatment groups and 8 replications in a completely random design. The environment temperature of region was 38 degrees Celsius for at least 4 hours per day. The control group received syrup without piperine, and the treatment groups received syrup containing 2000, 4000 and 6000 μg of piperine per liter, respectively. The level of malondialdehyde and antioxidant capacity of the body, weight and body composition of bees after emerging, colony population and relative expression of vitellogenin gene were determined.Results: Supplementation of 2000 and 4000 μg piperine per liter of syrup caused an increase in body weight, a decrease in the level of malondialdehyde and increase in antioxidant capacity. The addition of piperine in different doses caused a significant increase in vitellogenin gene expression compared to the control group. The spawning level and colony population that received 6000 μg/liter piperine was lower than the group that received 2000 and 4000 μg/liter P<0.05).Conclusion: Based on the results, supplementation of 2000 to 4000 μg/liter of piperine has a positive effect on increasing the antioxidant capacity, vitellogenin gene expression, body weight of new-emerged bees and colony population, and a higher dose causes a decrease in spawning rate and reduction of honey bee population. Manuscript profile
      • Open Access Article

        14 - Investigating relative water content, electrolyte leakage, phenolic compounds and activity of phenylalanine ammonia-lyase and tyrosine ammonia-lyase enzymes in triticale leaves treated with sodium chloride and calcium chloride
        Malihe Jahani Mohammad Reza Hadi Mojtaba Jafarinia Sedighe Jahani
        10.30495/zisti.2023.1981463.1157
        Introduction: Considering the increasing number of saline lands due to water scarcity, it seems necessary to investigate ways to increase tolerance to salinity stress. Also, in order to overcome the negative effect of salinity stress, the use of calcium ion supplement i More
        Introduction: Considering the increasing number of saline lands due to water scarcity, it seems necessary to investigate ways to increase tolerance to salinity stress. Also, in order to overcome the negative effect of salinity stress, the use of calcium ion supplement in the growth environment is very useful and practical to improve the stress factor.Objective: The purpose of this research was to investigate relative water content, electrolyte leakage, amount of mineral elements, antioxidant capacity, phenolic compounds and activity of phenylalanine ammonia-lyase and tyrosine ammonia-lyase enzymes in leaves of triticale (x Triticosecale Wittmack) treated with sodium chloride and calcium chloride.Materials and methods: This experiment was conducted as a completely randomized design with 3 replications in controlled greenhouse conditions. One-week-old seedlings were exposed with sodium chloride doses (0, 50, 100 and 150 mmol L-1) and calcium chloride doses (0, 6 and 10 mmol L-1) for 5 weeks and then some physio-morphological and biochemical characteristics including leaf number and surface, relative water content, ion leakage, contents of phenolic compounds (anthocyanin, total phenol, total flavonoid, total flavonol), enzymes activity (phenylalanine ammonia-lyase, tyrosine ammonia-lyase, glutathione reductase, glutathione S-transferase), antioxidant capacity and amount of mineral elements (sodium, potassium, calcium) were measured in leaves.Results: The results showed that salt stress significantly decreased the number and surface of leaves, relative water content, activity of glutathione reductase enzyme, the amount of potassium and calcium in leaves, but increased ion leakage, the contents of phenolic compounds, activity of phenylalanine ammonia-lyase, tyrosine ammonia-lyase and glutathione S-transferase enzymes, antioxidant capacity and sodium content in leaves. While the addition of calcium to saline environment increased the number and surface area of leaves, relative water content, activity of glutathione reductase enzyme, the amount of potassium and calcium in leaves, but decreased ion leakage, the contents of phenolic compounds, activity of phenylalanine ammonia-lyase, tyrosine ammonia-lyase and glutathione S-transferase enzymes, antioxidant capacity and sodium content in leaves.Conclusion: Calcium reduced the harmful effects of salinity stress in leaves of triticale plant, so adding calcium to salty soils can be a simple, practical and economic solution to deal with salinity stress and increase soil productivity and a step towards to provide sustainable agriculture. Manuscript profile
      • Open Access Article

        15 - Effect of ultrasound waves pretreatment on quantity and antioxidant capacity of extracted beta-carotene from carrot residue
        رویا Seyedifar نارملا Asefi یحیی Maghsoudlou
        Nowadays, the tendency towards the extraction of phytochemicals by ultrasound method has been increased. In this research, the effect of ultrasound waves pretreatment on extraction of beta-carotene from carrot residue was studied. Carrot residues were treated with ultra More
        Nowadays, the tendency towards the extraction of phytochemicals by ultrasound method has been increased. In this research, the effect of ultrasound waves pretreatment on extraction of beta-carotene from carrot residue was studied. Carrot residues were treated with ultrasound waves with 205 W power, constant 35 KHz frequency during  six stages of 0 (without ultrasound), 16, 20, 30, 40 and 44 min and at temperatures of 66, 70, 80, 90 and 94 ºC and using ethyl acetate as solvents. Then, the extract from each sample was evaluated considering the amount of total phenolic components as well as beta-carotene and antioxidant capacity of beta-carotene. According to the results, it was observed that, there was a significant difference (P<0.01) between samples in the case of beta-carotene amount with increasing of temperature and time. In order to achieve the highest extraction of carotene from carrot residues, the temperature of 90 ˚C and 40 minutes was determined as the best temperature and time. The results also revealed that at low temperatures the antioxidant capacity was higher. With increasing time of ultrasonic at low temperatures, the antioxidant capacity was enhanced. Moreover, the temperature of 70 ˚C and 40 min was determined as the optimum condition to achieve the highest antioxidant capacity of carrot residues. The result showed that the total phenolic compounds are greater at low temperature compared with high temperature. Besides, at all selected temperatures, with the increasing of ultrasonic duration the amount of total phenolics increased. Most phenols obtained at 66˚C for 40 min of ultrasonic treatment.   Manuscript profile
      • Open Access Article

        16 - The effects of active and inactivate Saccharomyces cerevisiae and their combination on performance, antioxidant levels and lipid peroxidation index in the blood of Japanese quail
        حسین نیک پیران حامد منافی توحید وحدت پور
        Considering that various effects of probiotics, prebiotics, and symbiotic on production and growth of broiler chicks has been reported previously, the aim of this study was to investigate the effects of probiotics and prebiotics and their mixture, on performance, antiox More
        Considering that various effects of probiotics, prebiotics, and symbiotic on production and growth of broiler chicks has been reported previously, the aim of this study was to investigate the effects of probiotics and prebiotics and their mixture, on performance, antioxidant levels and lipid peroxidation index in the blood of Japanese quails. Thus, 192 day- old- Japanese quails were distributed randomly to 4 groups with 4 replicates of 12 male and female and evaluated for 42 days. The control group only received basal diet, but  in the 2nd group (probiotic group) active saccharomyces cerevisiae 1 gr/Kg of feed, in the 3rd group (prebiotic group) inactivated saccharomyces cerevisiae 0.5 gr/Kg-feed of feed, and in the 4th group (synbiotic group) 0.5 gr/Kg saccharomyces cerevisiae and 250 mg/Kg-feed inactivated saccharomyces cerevisiae was added to the basal diet. Results indicated that mean serum levels of Malondialdehyde (MDA) was statistically different among males of different groups (p<0.05). Also the highest and lowest MDA levels were observed in the 4th and 3rd groups respectively with a statistically significant difference (p<0.05). Total antioxidant capacity (TAC) of plasma was not different statistically in the studied groups. On the other hand, mean feed consumption and body weight  in the 2nd and 3rd groups was increased in comparison to control group and  FCR was decreased significantly in comparison to control and synbiotic group. The best carcass efficiency was observed in the prebiotic group. The results showed that the employed probiotics and prebiotics were only effective in performance of Japanese quails, but did not have a specific effect on serum-MDA and plasma-TCA levels. Manuscript profile
      • Open Access Article

        17 - Protective and antioxidant effects of silymarin in sheep with fasciolosis treated by clorsulon
        Mehrzad Aflatooni negar panahi Pejman Mortazavi Bahar Shemshadi Shapoor Kakoolaki
        10.30495/jvcp.2021.1918402.1288
        Fasciolosis is a parasitic disease that causes economic losses. ‎In the present study, the protective effects of silymarin alone and in ‎combination with clorsulon were investigated in sheep naturally infected with ‎Fasciola hepatica. For this purpose, 40 Sa More
        Fasciolosis is a parasitic disease that causes economic losses. ‎In the present study, the protective effects of silymarin alone and in ‎combination with clorsulon were investigated in sheep naturally infected with ‎Fasciola hepatica. For this purpose, 40 Sangsari breed sheep ‎were used, which were divided into five groups, including 1- healthy sheep without treatment 2- ‎fasciolosis affected sheep without treatment 3- fasciolosis affected sheep  treated with silymarin 4- fasciolosis affected sheep treated with ‎Clorsulon, and 5- fasciolosis affected sheep treated with silymarin ‎and clorsulon. Biochemical parameters including triglyceride, total bilirubin, total protein, cholesterol, and albumin, were ‎measured. The serum level of malondialdehyde, total antioxidant capacity, and ‎hematological parameters were determined and compared among groups. Additionally, histopathological changes ‎were ‎‎‎investigated using light microscopy. Biochemical analysis showed significant ‎improvement in bilirubin levels, total protein, cholesterol, and albumin in sheep treated with silymarin ‎and clorsulon (p<0.05). Total antioxidant capacity increased in group treated with silymarin (p<0.05) compared to group 2, and the amount of oxidative stress decreased (p<0.01). Silymarin also improved hematologic factors and prevented a decrease in red blood cells (p<0.05).‎ Liver inflammation, ‎fibrosis, necrosis, and hyperplasia of the ‎bile ‎duct decreased significantly with the silymarin and ‎clorsulon group compared to the other groups ‎‎(p<0.0001). These findings ‎showed that using ‎silymarin in combination with clorsulon ‎improved their ‎therapeutic effects in treatment of ovine fasciolosis through enhancement of pharmacokinetic and pharmacodynamic relationship. Manuscript profile
      • Open Access Article

        18 - The effect of bio-fertilizers and Nano-nitrogen on strawberry quality and quantity under different levels of nitrogen
        Davood hashemabadi Mohammad hossain Namaki Farzin Saeeid zade
        Nitrogen is the most important element in plant growth and yield. There are several sources for supplying nitrogen needed by the plant, including the use of chemical fertilizers. But excessive use of chemical fertilizers in addition to contamination of water and soil ha More
        Nitrogen is the most important element in plant growth and yield. There are several sources for supplying nitrogen needed by the plant, including the use of chemical fertilizers. But excessive use of chemical fertilizers in addition to contamination of water and soil has also caused health risks to the community. Therefore, optimizing the use of chemical fertilizers and replacing them with organic sources can be a step forward in increasing the health of the community. For this purpose, a field experiment with different levels of urea fertilizer and various nitrogen sources were carried out in a factorial arrangement in a randomized complete block design with three replications on strawberry in 2016 at Someh-Sara, Gilan, Iran. The experimental factors included urea (0, 75 and 150 kg.ha-1) and various sources of nitrogen (Azospirilum, Azotobacter, Nano-nitrogen and Control). The results showed that in levels of nitrogen source, the application of urea fertilizer increased the shoot weight and shoot length, but decreased total antioxidant capacity, total flavonoid compounds and vitamin C. The highest fresh weight of shoot and fruit number were obtained from nano-nitrogen treatment + 75 kg.ha-1. While the highest amounts of vitamin C, total antioxidant capacity and total flavonoid compounds were obtained from Azotobacter and Azospirilum inoculated plants, Azotobacter was superior to Azospirilum in most of the traits and with increasing the application of urea fertilizer, maintained its efficiency compared to control. The highest fruit yield was obtained from nano-nitrogen + 75 kg.ha-1, but there was no statistically significant difference with Azotobacter + 75 kg.ha-1. Therefore, since the aim of the experiment was to increase the yield and quality of the fruit, Azotobacter + 75 kg.ha-1 is recommendable compared to other treatments. Manuscript profile
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        19 - Evaluation of some quantitative and qualitative properties of cornelian cherry (Cornus mas L.) fruit during the last stages of maturation
        mehdi panahi Jafar Hajilou Nader Chaparzadeh
        In recent years, increasing attention has been paid by consumers to cornelian cherry fruit, which is rich in antioxidants. This research was carried out based on a randomized complete block design for evaluation of the variations in some quantitative and qualitative pro More
        In recent years, increasing attention has been paid by consumers to cornelian cherry fruit, which is rich in antioxidants. This research was carried out based on a randomized complete block design for evaluation of the variations in some quantitative and qualitative properties of cornelian cherry fruits during the last stages of maturation in 2015. Fruits were harvested at 4 different times and from 4 main geographical directions of the tree and were then immediately transferred to the laboratory. Several parameters such as dimension (length and width), weight, firmness, pH, total soluble solids (TSS), total titratable acidity (TA), TSS/TA ratio, ascorbic acid (vitamin C), total phenolic compounds, total flavonoids, total anthocyanins, and total antioxidant capacity of the fruits were investigated. The results showed that the effect of different harvest dates on all traits was significant (P<0.05) while no significant differences were observed between replications. During fruit ripening, weight, dimension, pH, TSS, TSS/TA ratio, total flavonoids, and total anthocyanins increased while firmness, TA, total phenol, and total antioxidant capacity decreased. The content of ascorbic acid increased during fruits ripening, but it reduced again in the last harvest. The results indicate that the third harvest time (16.63 Brix level) can be the best harvest date for this genotype. Manuscript profile
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        20 - Variation on biochemical, phytochemical and genetic diversity of fig (ficus carica) from East Azerbaijan province
        akbar ghorbani hamid Hasanpour sezai Ercisli
        Abstract. Fig (Ficus cariaca) is native to western and eastern mediterranean regions such as western and northwestern of Iran. Iran is one of the important fig producers in the world. In this study, biochemical, phycochemistry characteristics and genetic diversity of 38 More
        Abstract. Fig (Ficus cariaca) is native to western and eastern mediterranean regions such as western and northwestern of Iran. Iran is one of the important fig producers in the world. In this study, biochemical, phycochemistry characteristics and genetic diversity of 38 fig genotypes from Arsbaran in East Azerbaijan province in the Horticulture Laboratory of Urmia University in 2015 were investigated. Biochemical results showed that, TSS, pH, TSS/TA, vitamin c, antioxidant capacity, total anthocyanin, total flavonoid and total phenol were significant (p ≤ 0.01). Most of the studied biochemical traits indicated high variation. The results of the molecular data showed that a total of 131 bands were scored, of which 121 bands (92%) were polymorphic. The amount of polymorphic (PIC) information for the primers used varied from 0.22 to 0.47 in ISSR7 and ISSR20 respectively. Also, marker index were between 0.15 to 2.5 in ISSR7 and ISSR20 respectively. Based on the results of cluster analysis, the studied fig genotypes were classified into two main groups, so that the most of collected genotypes from Kalibar belonged to second group. So it could be concluded that geographic location of the studied fig genotypes is the main reason for their separation by this marker. Based on obtained results, the genotypes Kh9 and H17 were suggested for breeding programs. Manuscript profile
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        21 - Interaction of harvest time and storage period on physiological and biochemical parameters of kiwi fruit (Actinidia deliciosa cv. Gold)
        Mahmoud Asadi somayeh Rohani Javad Fatahi moghadam Babak Babakhani Parvaneh Rahdari
        Proper harvesting time plays an important role in maintaining fruit quality during storage. Early or delayed harvesting results in unpleasant taste, inability to store and inappropriate appearance. In order to evaluate the effects of harvest time and storage period on p More
        Proper harvesting time plays an important role in maintaining fruit quality during storage. Early or delayed harvesting results in unpleasant taste, inability to store and inappropriate appearance. In order to evaluate the effects of harvest time and storage period on physiological and biochemical parameters was conducted with three replications. Tests were carried out with four harvesting time (Based on the total soluble solids (TSS) index), i.e., 5, 6.5, 8 and 9.5 °Brix and four storage time, i.e., 0, 30, 60 and 90 days. Based on the experimental treatments, traits such as fruit juice, TSS, TA, TSS/TA ratio, starch and protein content, fruit pigments, phenolic compounds, antioxidant activity, vitamin C, total sugar content and reducing sugars content in fruit were measured. The results showed that in all harvesting treatments, increasing the storage period significantly increased TSS, TA, protein content, total phenol, vitamin C, total sugar content, reducing sugar and antioxidant capacity. Chlorophyll a, b, total chlorophyll and carotenoid contents increased significantly with increasing harvest index and storage period. The results of this study showed that 60 and 90 days storage periods play an important role in increasing physiological and biochemical indices of kiwifruit (except traits such as fruit juice, starch content, TSS/TA ratio and superoxide dismutase activity) in all harvest treatments. Manuscript profile
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        22 - Effect of size and canopy position on physicochemical properties, carbohydrate, and bioactive compounds of Thomson Navel orange (Citrus sinensis cv. Thomson Navel)
        somayeh Rezaei Javad Fatahi moghadam Parvaneh Rahdari Babak Babakhani Mahmoud Asadi
        10.30495/iper.2021.679533
        Citrus fruits contain numerous beneficial bioactive compounds including vitamin C, phenolic compounds, flavonoids, carotenoids, and sugars which have high nutritional and antioxidant value. Some factors such as the canopy position of the fruit and fruit size affect the More
        Citrus fruits contain numerous beneficial bioactive compounds including vitamin C, phenolic compounds, flavonoids, carotenoids, and sugars which have high nutritional and antioxidant value. Some factors such as the canopy position of the fruit and fruit size affect the quality and quantity of these compounds. The aim of this study was to investigate the effect of canopy position and fruit size on morphophysiological and biochemical properties of Thomson orange fruit. In the first experiment, fruits with medium and uniform size were harvested from inside and outside canopy positions of the tree. In the second experiment, after harvest the fruits were grouped into three sizes of small, medium, and large. Traits such as weight, volume, length, and width of fruit, skin color, skin thickness, extract content, dry matter percentage, TA, TSS, TSS/TA, total chlorophyll and total carotenoids, antioxidant capacity, phenolic acid content, vitamin C, total sugars, and reducing sugars were evaluated on the peel and pulp of fruit along with a panel test. Results revealed that fruit volume, peel thickness, TSS/TA, a* value, CCI value, peel antioxidant capacity, and peel total sugars were higher in the fruits from the external canopy position while TA, L*, C*, h°, and b* in the fruits from internal canopies were significantly higher. Fruit size had a significant effect on physical parameters of fruit, CCI, h°, juice, and dry matter while it did not affect the quality and chemical traits of fruit. The results of this study showed that orange fruit with a position outside the crown and a large size has a significant increase in extract content, actual volume, average fruit diameter, aroma, taste, and sweetness compared to the position inside the crown with a small size. Manuscript profile
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        23 - The effect of solvent type and concentration on extraction of phenolic compounds and evaluation of antioxidant activity of Crataegus elbursensis L. leaf collected from Golestan province
        Mohammad Moghaddam Leila Mehdizade
        10.30495/iper.2021.679541
        In order to investigate the effect of the type and concentration of solvent on the amount of extraction of phenolic compounds and antioxidant activity of Crataegus elbursensis leaves, a research was performed in two separate experiments. At first experiment the effect o More
        In order to investigate the effect of the type and concentration of solvent on the amount of extraction of phenolic compounds and antioxidant activity of Crataegus elbursensis leaves, a research was performed in two separate experiments. At first experiment the effect of solvent type (acetone, ethanol, methanol) at three concentrations (50, 80 and 100%) on extracted total phenolics content of C. elbursensis leaf was evaluated. At second experiment antioxidant capacity of produced extract from the best solvent at first experiment with the highest phenolic compounds (methanol 80%) was investigated by two different methods including total antioxidant and Fe reduction capacity. The results of these experiments showed that all three solvents; acetone, ethanol and methanol; in the form of mixture with water have more potential for extracting phenolic compounds toward the pure ones. The highest total phenolic content (118 mg GAE/g DW) was obtained at 80% concentration of all solvents, especially at methanol 80%. The results of evaluating antioxidant activity showed that with increasing the concentration of extract up to 500 µg/mL, antioxidant activity (0.8 mg/mL) was increased. Investigation of Fe reduction capacity indicated that with increasing the concentration of extract up to 800 µg/mL, the amount of absorption of the solvents contain the extract significantly increased. Therefore, according to the results of this study, the leaves of this plant can be used as the source of phenolics and antioxidants in different industries. Manuscript profile
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        24 - Effects of different concentrations of chitosan bio-stimulant on some physiological and biochemical traits of Andrographis paniculate L.
        Mozhdeh Jafari Lafoot Leila Pishkar Daryush Talei
        10.30495/iper.2022.1944978.1747
        Chitosan is a nitrogen containing polysaccharide synthesized naturally by deacetylation reaction of chitin, which is confirmed as an efficient bio-stimulant to improve production of secondary metabolites in medicinal plants. In the present study, the effects of chitosan More
        Chitosan is a nitrogen containing polysaccharide synthesized naturally by deacetylation reaction of chitin, which is confirmed as an efficient bio-stimulant to improve production of secondary metabolites in medicinal plants. In the present study, the effects of chitosan (0, 100, 200, 300, and 400 mg/L) on growth, photosynthetic pigments, secondary metabolites, antioxidant capacity, activity of antioxidant enzymes, quantity and quality of protein, and the expression of HMGS and HMGR genes were examined in Andrographis paniculata L. The results showed that application of chitosan significantly increased the chlorophyll and carotenoids and, as a result, increased plant growth and biomass. Chitosan treatments (300 and 400 mg/L) increased the expression of HMGS and HMGR genes in plant leaves, which was associated with a significant increase in the accumulation of total phenols and flavonoids. Also, chitosan improved the activity of superoxide dismutase and peroxidase enzymes and increased the antioxidant capacity of A. paniculata leaves. Examination of the quantity and quality of proteins showed that the intensity of protein bands with the molecular weight of 60 kD under 300 and 400 mg/L chitosan treatment and also the intensity of protein bands with the molecular weights of 35 and 20 kD under 300 mg/L chitosan treatment increased compared to the other concentrations. Therefore, these results revealed that application of chitosan can increase accumulation of secondary metabolites and the antioxidant capacity in the medicinal plant A. paniculata. Manuscript profile
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        25 - Investigation of the effect of silica spraying and salinity stress on some physiological traits of Camelina sativa oil plant
        Ebrahim Fani Shokoofeh Hajihashemi
        10.30495/iper.2022.1954207.1780
        In order to investigate the effects of salinity stress and foliar application of silica on Camelina sativa oil plant, a factorial study was carried out in the growth chamber of the laboratory of the Department of Biology, Behbahan Khatam Alanbia University of Technology More
        In order to investigate the effects of salinity stress and foliar application of silica on Camelina sativa oil plant, a factorial study was carried out in the growth chamber of the laboratory of the Department of Biology, Behbahan Khatam Alanbia University of Technology, in 3 replications based on a completely randomized block design. Treatments included salinity stress at two levels (0 and 100 mM NaCl) and potassium silicate solution at two levels (0 and 5 mM). The physiological and biochemical traits of Camelina sativa under salinity stress were evaluated along with the role of osmotic substances such as soluble sugar and soluble protein in reducing the harmful effects of salinity stress. Results showed that salinity stress significantly reduced the amount of chlorophyll a, b, and total in Camelina sativa, while silica treatment significantly reduced the harmful effects of salinity. Soluble sugar content of leaves in response to salinity stress was 46% lower than in the control plant. Salinity stress combined with silica increased the amount of soluble sugar 27% more than the control plant. Results also showed that in the treatment with silica under 100 mM salinity level, the amount of leaf proteins was almost 10% less than the control plants. The results of measuring the plant’s antioxidant capacity of FRAP showed that in response to silica treatment without salinity, no significant difference was observed in the amount of antioxidant capacity of FRAP, while in the combined treatment of salinity stress + silica it decreased by 9% compared with the control plants. The results of this study supports the beneficial role of silica as an environmentally friendly compound to increase plant resistance to salinity stress. Manuscript profile
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        26 - ارزیابی تأثیر متغیر های فراصوت بر استخراج ترکیبات فنولی موجود در پوست سبز بادام (Prunus amygdalus)
        ویکتوریا مسعودی اعظم اعرابی
        مقدمه و هدف: استخراج ترکیبات فنولی از باقی مانده محصولات کشاورزی برای توسعه محصولات با ارزش افزوده مهم است. بادام(Prunusamygdalus)یکی از محصولات کشاورزی بومی کشور است که به مقدار زیاد در کشور پرورش داده می‌شود. در این مطالعه از پوست سبز بادام به­ عنوان یک محصول More
        مقدمه و هدف: استخراج ترکیبات فنولی از باقی مانده محصولات کشاورزی برای توسعه محصولات با ارزش افزوده مهم است. بادام(Prunusamygdalus)یکی از محصولات کشاورزی بومی کشور است که به مقدار زیاد در کشور پرورش داده می‌شود. در این مطالعه از پوست سبز بادام به­ عنوان یک محصول جانبی که امروزه صرفاً برای تغذیه دام استفاده می‌شود جهت بررسی استخراج ترکیبات فنولیک و خاصیت آنتی­اکسیدانی آنها استفاده شده است. روش تحقیق: در این تحقیق استخراج ترکیبات فنولی با استفاده از امواج فراصوت به روش سطح پاسخ و با کمک نرم­ افزار مینی تب مورد بررسی قرار گرفته و اثر چهار پارامتر دما، زمان تیمار با فراصوت، فرکانس امواج فراصوت و غلظت اتانول هر کدام در پنج سطح بر میزان استخراج ترکیبات فنولی مورد بررسی و بهینه‌سازی قرار گرفت. در نهایت تاثیر نسبت حلال به ماده جامد بر میزان استخراج این ترکیبات بررسی شده م با روش متداول سوکسله مورد مقایسه قرار گرفت. نتایج و بحث: نتایج آزمون­ها بر اساس طراحی انجام شده نشان داد که مقدار بهینه استخراج در شرایطی که اتانول 86 %، دما 50 درجه سانتی‌گراد، زمان 75 دقیقه و فرکانس 163هرتز باشد به‌دست می‌آید. همچنین تأثیر نسبت حلال به ماده جامد (پوست بادام ) در نسبت­های (w/w 1:10، 1:20، 1:30، 1:40) طبق شرایط بهینه به­ دست آمده در مرحله قبل با امواج فراصوت ارزیابی گردید و ظرفیت آنتی­اکسیدانی عصاره­ های استخراج شده در این شرایط با روش مهار رادیکال آزادDPPHاندازه­گیری گردید. نتایج نشان داد بیشترین مقدار ترکیبات فنولیک و ظرفیت آنتی اکسیدانی در نسبت 40:1 حلال به ماده جامد به ترتیب برابر 19/1134 میلی­گرم بر گرم ماده خشک و 42/87 درصد به دست آمد. نتایج این نسبت از حلال و ماده جامد با نتایج به­دست آمده از استخراج ترکیبات فنولیک با روش سوکسله و در حضور اتانول خالص تفاوت معناداری را در ترکیبات فنولیک و ظرفیت آنتی‌اکسیدانی آن نشان داد. توصیه‌های کاربردی/ صنعتی:. کاربرد تکنیک‌های جدید از جمله امواج فراصوت در استخراج ترکیبات فنولی از منابع گیاهی می‌تواند راهکار موثری نه تنها برای افزایش میزان استخراج این ترکیبات باشد بلکه به دلیل زمان کوتاهتر، آسیب کمتری نیز به این ترکیبات وارد می‌شود. Manuscript profile
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        27 - فرمولاسیون و خصوصیات یک دسر لبنی کاربردی حاوی عصاره Moringa oleifera و اینولین
        پویا حسن نژاد زهرا بیگ محمدی نفیسه جهانبخشیان
        10.30495/medherb.2023.708213
        Background & Aim: Today, due to the importance of nutrition and the role of food on human health, attention has been paid towards the production of foods rich in functional and beneficial compounds, as well as low calorie products to reduce various diseases such as More
        Background & Aim: Today, due to the importance of nutrition and the role of food on human health, attention has been paid towards the production of foods rich in functional and beneficial compounds, as well as low calorie products to reduce various diseases such as diabetes, obesity, cardiovascular diseases, etc. The aim of this research was to develop a functional and low-calorie dairy dessert using long-chain inulin and Moringa oleifera extract.Experimental: Moringa oleifera extract (ME) was prepared by the percolation method and used in combination with inulin in the formulation of low-calorie dairy dessert based on maltitol and sucralose as sweeteners. ME was used at 1%, 2% and 3% (w/w) levels and inulin at 5% and 7% levels. The moisture, ash, fat, protein and carbohydrate content, as well as calorie, pH, acidity, soluble solids (brix), syneresis, viscosity, color indices, antioxidant capacity and sensory characteristics (color, flavor, texture, mouth feel and overall acceptability) of the dairy dessert treatments were examined after production.Results: The addition of inulin and ME showed a significant effect on the chemical composition of the desserts and reduced the calories of the treatments compared to the control. Increasing the level of ME did not affect the syneresis and viscosity of the desserts, while by increasing the level of inulin from 5% to 7%, viscosity increased and syneresis decreased significantly (P<0.05). Incorporation of ME into the formulation of dairy dessert also reduced the L* and a* and increased the b* values of the samples (P<0.05). By adding ME and increasing its level from 1% to 3% in the formulation, significant improvement in the antioxidant capacity of the desserts was observed, so that the highest antioxidants capacity was evaluated in the samples containing 7% ME (37.53-37.82%). The results of sensory evaluation indicated that all treatments were acceptable, and the desserts containing lower levels of ME obtained higher flavor, color and overall acceptability scores.Recommended applications/industries: The results of this research demonstrated that inulin as a prebiotic and Moringa oleifera extract as a rich source of bioactive and antioxidant compounds can be used to develop a functional low-calorie dairy dessert. Manuscript profile
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        28 - بررسی تنوع ناحیه ای در کلکسیون عناب ایرانی بر اساس ظرفیت پاداکسایشی و ویژگیهای فنلیک
        علی عزیزی مریم پیربداقی
        مقدمه و هدف: میوه های گیاه عناب(Ziziphus jujuba) دارای ویژگیهای سلامتی بخش و خواص دارویی است. استفاده از عناب  به عنوان یک گیاه دارویی برای درمان برخی بیماریها در طب سنتی ایرانی تاریخ دیرینه ای دارد. در این پژوهش تنوع میان 29 نمونه ثبت شده از هفت استان موجود د More
        مقدمه و هدف: میوه های گیاه عناب(Ziziphus jujuba) دارای ویژگیهای سلامتی بخش و خواص دارویی است. استفاده از عناب  به عنوان یک گیاه دارویی برای درمان برخی بیماریها در طب سنتی ایرانی تاریخ دیرینه ای دارد. در این پژوهش تنوع میان 29 نمونه ثبت شده از هفت استان موجود در کلکسیون عناب ایران بر اساس ویژگیهایی شامل ظرفیت پاداکسایشی، محتوای فنل کل، فلاونوئید و آنتوسیانین کل مورد مطالعه قرار گرفت. روش تحقیق: عصاره گیری از میوه های عناب با استفاده از متانل (85%) انجام شد. آنالیز DPPH جهت اندازه گیری ظرفیت پاداکسایشی مورد استفاده قرار گرفت. جهت سنجش میزان فنل کل، روش مبتنی بر  فولین سیکالتیو به کار رفت و مقدار فلاونوئید کل بر اساس روش رنگ سنجی کلرید آلومنیوم تعیین شد. برای اندازه گیری محتوای آنتوسیانین کل از روش ضریب تغییر اسیدیته استفاده شد. نتایج و بحث: نتایج آنالیز واریانس نشان داد که نمونه های گیاهی از عناب های مورد آزمایش، یک تفاوت و گوناگونی بالایی(با سطح احتمال ا%) در تمامی صفات فیتوشیمیایی اندازه گیری شده دارند. آنالیز تجزیه به مولفه های اصلی(PCA) بر اساس چهار ویژگی مذکور این تنوع گسترده را تایید کرد و همچنین نمونه های گیاهی را به سه گروه مجزا تفکیک نمود. نمونه گیاهی حمیدآباد ساری (مازندران)، بیاضیه (اصفهان) و کوهپایه 2 (اصفهان) به ترتیب در ویژگیهای محتوای فنل کل، فلاونوئید و آنتوسیانین کل نسبت به بقیه گیاهان برتری داشتند. از طرف دیگر نمونه اردستان (اصفهان)، قاضی اولیا (قم) و مارون (قم) ظرفیت پاداکسایشی بالا داشتند. رابطه همبستگی معنی داری بین محتوای فنل کل و ظرفیت پاداکسایشی یافت نشد. توصیه کاربردی- صنعتی: یافته های این پژوهش نشان داد که ژنوتیپ و مکان رویش عناب تاثیر زیادی بر پتانسیل پاداکسایشی و ویژگیهای فنلیک آن دارد. آگاهی از تنوع یافت شده در این پژوهش، می تواند در بهره برداری بهتر این گیاه در داروسازی و هدایت بهتر برنامه های بهنژادی عناب کمک نماید. Manuscript profile
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        29 - Effects of Hydrolyzed Cottonseed Protein on Growth Performances, Carcass Traits, Immunity, Microbial and Morphological Responses of the Small Intestine and Total Antioxidant Capacity of Serum, and Small Intestine in Broiler Chickens
        N. Landy F. Kheiri
        Manuscript profile
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        30 - Total Antioxidant Capacity and Malondialdehyde Level in Plasma of Broiler Chicks Fed Diet Containing Different Levels of Ginger (Zingiber officinale)
        A.A. Sadeghi W. Izadi P. Shawrang M. Chamani M. Aminafshar
        Manuscript profile
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        31 - تأثیر عصاره رزماری (Rosmarinus officinalis) بر عملکرد، توانایی آنتی‌اکسیدانی و فراسنجه‌های گازهای خونی جوجه‌های گوشتی تحت تیمار نیترات سدیم در آب آشامیدنی
        ع.ر. اخواست م. دانشیار
        دویست و بیست جوجه گوشتی در 5 گروه برای بررسی تأثیر سطوح مختلف 0، 5/1، 3 و 6 میلی‌لیتر عصاره رزماری همراه با نیترات سدیم (4/27 میلی‌گرم در لیتر آب آشامیدنی) در مقایسه با آب شاهد (فاقد هیچ افزودنی) بر عملکرد و پتانسیل آنتی‌اکسیدانی جوجه‌های گوشتی استفاده شد. افزایش وزن بد More
        دویست و بیست جوجه گوشتی در 5 گروه برای بررسی تأثیر سطوح مختلف 0، 5/1، 3 و 6 میلی‌لیتر عصاره رزماری همراه با نیترات سدیم (4/27 میلی‌گرم در لیتر آب آشامیدنی) در مقایسه با آب شاهد (فاقد هیچ افزودنی) بر عملکرد و پتانسیل آنتی‌اکسیدانی جوجه‌های گوشتی استفاده شد. افزایش وزن بدن و ضریب تبدیل خوراک در طول دوره پایانی به طور منفی تحت تأثیر نیترات قرار گرفت که با سطوح مختلف عصاره رزماری به حالت عادی برگشت. هر دوی اسید اوریک و ظرفیت کل آنتی‌اکسیدانی توسط نیترات کاهش یافت در حالیکه با مصرف 3 میلی‌لیتر عصاره رزماری به سطح شاهد رسید. نیترات فشار جزئی اکسیژن را کاهش داد و سطوح 3 و 6 میلی‌لیتر در لیتر عصاره رزماری فشار جزئی اکسیژن را به سطح کنترل رساند. فشار جزئی دی‌اکسید کربن خون سیاهرگی بر خلاف فشار جزئی اکسیژن تحت تأثیر نیترات و عصاره رزماری قرار گرفت. به طور کلی، مصرف عصاره رزماری در آب آشامیدنی می‌تواند توانایی آنتی‌اکسیدانی و عملکرد جوجه‌های گوشتی تحت تیمار نیترات سدیم را بهبود دهد Manuscript profile
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        32 - Effects of Horsetail (Equisetum arvense), Vitamin C and Organic Zinc Supplements on Growth Performance, Carcass Yield, Serum Biochemical Values and Antioxidant Status of Broiler Chickens
        S. Azimi-Youvalari M. Daneshyar S.M. Abtahi-Froushani S. Payvastegan
        Manuscript profile
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        33 - Plant growth promoting rhizobacteria (PGPR) improve plant growth, antioxidant capacity, and essential oil properties of lemongrass (Cymbopogon citratus) under water stress
        Mitra Mirzaei Alireza Ladan Moghadam Leila Hakimi Elham Danaee
        10.30495/ijpp.2020.672574
        Manuscript profile
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        34 - Evaluation of Antioxidant Properties of Bran and Grain in 10 Genotypes of Rice (Oryza Sativa L)
        Mohammadreza Jafari Talobaghi Morteza Sam daliri Pouria Mazloum Valiollah Rameeh Morteza Moballeghi
        10.30495/ijpp.2020.1912117.1258
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        35 - Physiological and biochemical changes of Alfalfa (Medicago sativa L.) cultivars during different growth stages
        Alireza Khedri Behzad Sani behnam Zand Hamid Mozafari Payam Moavni
        10.30495/ijpp.2022.1948975.1396
        Manuscript profile
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        36 - تاثیر اسید جیبرلیک و نیتروژن بر ویژگی‌های خوراکی بنفشه بومی (Viola odorata) و تجاری (Viola tricolor)
        ندا نکویار روح انگیز نادری داود هاشم آبادی علی محمدی ترکاشوند
        پرورش گل‌های خوراکی از جمله بنفشه که بومی ایران است راهکاری مناسب جهت افزایش دسترسی بشر به این منابع جدید و با ارزش غذایی است. در این راستا در پژوهش حاضر اثر متقابل اسید جیبرلیک (GA) (0، 150 و 300 میلی‌گرم در لیتر) و نیتروژن (N) (0، 100 و 200 میلی‌گرم در لیتر) روی ویژگی More
        پرورش گل‌های خوراکی از جمله بنفشه که بومی ایران است راهکاری مناسب جهت افزایش دسترسی بشر به این منابع جدید و با ارزش غذایی است. در این راستا در پژوهش حاضر اثر متقابل اسید جیبرلیک (GA) (0، 150 و 300 میلی‌گرم در لیتر) و نیتروژن (N) (0، 100 و 200 میلی‌گرم در لیتر) روی ویژگی‌های دو گونه بنفشه (Viola tricolor and Viola odorata) بصورت آزمایش فاکتوریل در قالب طرح کاملا تصادفی در سه تکرار بررسی شد. نتایج نشان داد که کاربرد اسید جیبرلیک و نیتروژن بطور معناداری موجب بهبود ویژگی‌های خوراکی در هر دو رقم بنفشه می‌شود. بطوری‌که بیشترین پروتئین، کاروتنوئید، آهن و روی گلبرگ در هر دو گونه بنفشه با کاربرد "GA300 × N200" بدست آمد. مقدار آنتوسیانین گلبرگ در بنفشه معطر با کاربرد "GA0 × N100" (58.32 mg 100g-1 F.W.) و در بنفشه تجاری با کاربرد "GA300 × N100" (66.84 mg 100g-1 F.W.) بیشترین مقدار بود. بنفشه تجاری از نظر سلنیوم غنی‌تر از بنفشه معطر بود و تیمارهای "GA300 × N200"، "GA300 × N100" و "GA150 × N200" بیشترین غلظت سلنیوم را در بنفشه تجاری بخود اختصاص دادند. در هر دو نوع بنفشه بیشترین فلاونوئید طول موج‌های 300 و 330 نانومتر با کاربرد "GA300 × N200" و "GA150 × N200" بدست آمد. اما اثر تیمارها روی فلاونوئید 270 نانومتر معنی‌دار نبود. ظرفیت آنتی‌اکسیدانی بنفشه معطر بیشتر از بنفشه تجاری بود و بیشترین ظرفیت آنتی اکسیدانی در بنفشه معطر (84.83 % DPPHsc) و بنفشه تجاری (78.17 % DPPHsc) با کاربرد "GA150 × N200" حاصل شد. با توجه به نتایج حاصل، دو رقم بنفشه مورد مطالعه به‌عنوان منابع موثری از پروتئین، عناصر معدنی و ترکیبات آنتی‌اکسیدانی معرفی می‌شوند. همچنین کاربرد "GA300 × N200" و "GA150 × N200"جهت بهبود ویژگی‌های خوراکی این دو گونه گیاهی پیشنهاد می‌شود. Manuscript profile
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        37 - Effect of low temperature stress on biochemical and physiological characteristics of some native genotypes of like-lime in the north of Iran
        Saleh Mohammadi hamidreza khazaie Ahmad Nezami Yahya tajvar
        In this study, the vulnerability of six native genotypes of like-lime (1-6 number) with control tolerant variety of Unshiu and sensitive variety of Persian lime (to low temperature) were studied low temperature stress ( 3, 0, -3 and -6˚C), on physiological responses, in More
        In this study, the vulnerability of six native genotypes of like-lime (1-6 number) with control tolerant variety of Unshiu and sensitive variety of Persian lime (to low temperature) were studied low temperature stress ( 3, 0, -3 and -6˚C), on physiological responses, in a completely randomized design with Tukey´s mean comparison. Analysis of variance showed that in the interaction between genotype and temperature, leaf water soaking, electrolyte leakage, lipid peroxidation, proline content and antioxidant capacity were significant. It was while in reaction of temperature and genotype, total carbohydrates, chlorophyll a, b, total and Carotenoids have not significant differences. Therefore, in comparison of data, the highest of leaf water soaking (91/628%), electrolyte leakage (99.3%) and lipid peroxidation (3.32 µgr/gr FW) were recorded in control sensitive plant (Persian lime) at temperature of -6˚C. Versus, the highest proline content (32.006 mg/gr leaf fresh Weight) and antioxidant capacity (73.36%) were observed in control tolerant plant (Unshiu) at temperature of -3˚C. It is noteworthy that total carbohydrate, chlorophyll a and total, were significantly different only under the influence of genotype factor. Accordingly, the maximum amount of chlorophyll a (2.359 mg/gr leaf fresh Weight), total chlorophyll (2.932 mg/gr leaf fresh Weight) and total carbohydrate (33.486 mg/gr leaf fresh Weight) were measured in in control tolerant plant (Unshiu). It should be noted that among the six native genotypes of like-lime studied, different reactions were recorded under low temperature. So that after control tolerant plant (Unshiu), native genotypes like-lime No. 6 showed better resistance against low temperature stress. Manuscript profile
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        38 - Effects of Pre-germination Treatment on the Phytate and Phenolic Contents of Almond Nuts
        Liang Lin Lin Freda Xin You Giam Xin Min Foo Nadia Marie Hui Lian Yeo Charlene Jia Ling Koh Nur Hatika Binte Sa’Aban Wai Mun Loke
        10.22034/jon.2017.530394
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        39 - Evaluation of Oxidative Stress Biomarkers in Patients with Breast cancer Compared with Healthy Subjects
        Hosnie Hoseini Masoud Kerman Saravi Shirin Shahraki Azade Sarani Afsane Sarabandi
        10.22034/jchr.2021.1922854.1258
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        40 - Evaluation of Aloe vera (Aloe barbadensis Miller) Antioxidant Activity and Some of the Morphological Characteristics in Different Vermicompost Field
        Z. Yavari H. Moradi H. Sadeghi B. Barzegar Golchini
        10.22034/jchr.2018.544044
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        41 - Effects of Pre-harvest Salicylic Acid Treatment on the Post-harvest Quality of Peach Cultivar Robin
        Ramin Salyari Esmaeil Seifi Feryal Varasteh Mahdi Alizadeh
        10.22034/jchr.2021.1875114.1041
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        42 - Investigation of Seed Germination, Early Growth and Physio-Biochemical Parameters of Canola Seedling Exposed to Co3O4 Engineered Nanoparticles
        Malihe Jahani Ramazan Ali Khavari-Nejad Homa Mahmoodzadeh Sara Saadatmand
        10.22034/jchr.2020.1891185.1092
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        43 - Assessment of Biomarkers of Oxidative Stress among Patients with Lymphoma
        Hosnie Hoseini Parichehreh Yaghmaei Gholamrezagh Bahari Saeed Aminzadeh
        10.22034/jchr.2021.1916955.1228
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        44 - The Influence of Zinc Oxide Nanoparticles on Blood Markers in Domestic Pigeons (Columba livia)
        Mehran Arabi Hamid-Reza Naseri
        10.22034/jchr.2021.1937480.1379
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        45 - The Effect of High-intensity Interval Training and High-Protein Diet on the Total Oxidant Status and Total Antioxidant Capacity in Cardiac Tissue of Obese Male Rats
        Azin Ghane Hossein Abednatanzi Marziyeh Saghebjoo Mehdi Hedayati
        10.22034/ascij.2022.1955860.1378
        Obesity causes oxidative stress and is a risk factor for cardiovascular diseases. Exercises and diet modification can affect oxidant and antioxidant factors. The aim of this study was to investigate the effect of high-intensity interval training (HIIT) and high-protein More
        Obesity causes oxidative stress and is a risk factor for cardiovascular diseases. Exercises and diet modification can affect oxidant and antioxidant factors. The aim of this study was to investigate the effect of high-intensity interval training (HIIT) and high-protein diet (HPD) on total oxidant status (TOS), total antioxidant capacity (TAC) and the ratio of TAC to TOS in the heart tissue of obese male rats. Forty 12-week-old obese male rats (Lee index above 310) were divided into five groups of eight including HIIT (5-12 intervals of 30 seconds with an intensity of 90% VO2max, 5 sessions per week, 10 weeks), HPD (about 57.5% of total calories from protein), HIIT+HPD, obese control-1 (OC-1) and obese control-2 (OC-2) and eight non-obese mice were also divided into the non-obese control (NC) group. became After 48 hours from the last intervention session, heart tissue was removed to measure TAC and TOS levels. Data were analyzed using one-way analysis of variance, post hoc Tukey, Kruskal-Wallis and Mann-Whitney tests at a significance level of p ≤ 0.05. The results showed that the TAC level of heart tissue in the HIIT, HPD, HIIT+HPD groups was significantly higher than in the OC-1 group. On the other hand, the TOS level of heart tissue was significantly lower in the HIIT and HIIT+HPD groups than in the HPD and OC-2 groups. Also, the ratio of TAC to TOS was significantly higher in the HIIT and HIIT+HPD groups compared to the OC-2 group. According to the results, a combination of HIIT and HPD improves TAC and TOS of heart tissue. It seems that HIIT together with HPD strengthens the antioxidant system and reduces oxidative stress in obesity conditions. Manuscript profile
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        46 - Effect of Pomegranate Supplementation on Antioxidant Status in Young Women after Heavy Resistance Exercise
        Babak Hooshmand Moghadam Sirous Choobineh
        Today, researchers are attracted to focus on the prevention of production of free radicals, and the consumption of antioxidants, especially polyphenols. Therefore we aimed to investigate the effect of pomegranate juice supplementation on total antioxidant capacity (TAC) More
        Today, researchers are attracted to focus on the prevention of production of free radicals, and the consumption of antioxidants, especially polyphenols. Therefore we aimed to investigate the effect of pomegranate juice supplementation on total antioxidant capacity (TAC) and malondialdehyde (MDA) in young women after a session of heavy resistance exercise. In this quasi-experimental design, 20 young women with the inclusion criteria were randomly divided into two groups. The first group received 250 ml normal pomegranate juice daily and the second received 250 ml of placebo for two weeks each day. After two weeks, both groups participated in a session of heavy resistance exercise. Blood samples were taken at the beginning and end of the study after 12 h of fasting. TAC and MDA were measured by TBARS and FRAP reactions respectively, then analyzed by independent t-test at P≤0.05 level. Pomegranate juice supplementation group had a significant increase in the TAC after heavy resistance exercise (P =0.038). However, this change was not significant in the placebo group. MDA in pomegranate juice supplementation group showed a significant decrease after heavy resistance training (P=0.016). This change was not significant in the placebo group. Taking supplements two weeks of heavy resistance exercise can improve total anti-oxidative capacity and reduce lipid peroxidation in young women. Manuscript profile
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        47 - The Effect of Lycopene Supplement on Oxidative Stress and Total Antioxidant Capacity in Obese Men after Resistance Exercise
        B. Hooshmand moghadam M. Eskandari
        The effect of supplementation with lycopene (tomato juice) on the TAC and MDA in obese men after resistance exercise was examined. In this study, 20 obese men (BMI ≥ 30) were randomly assigned to one of two groups: placebo (n = 10) and supplements (n = 10). Group sup More
        The effect of supplementation with lycopene (tomato juice) on the TAC and MDA in obese men after resistance exercise was examined. In this study, 20 obese men (BMI ≥ 30) were randomly assigned to one of two groups: placebo (n = 10) and supplements (n = 10). Group supplements daily for two weeks 2 tablets of 200 mg lycopene and the placebo group the amount received placebo. Then the two groups participating in resistance exercise and venous blood samples at three stages: before supplementation (2 wk before the activity), before, and 24 h after the exercise was collected. The TAC and MDA concentration of biomarker in the supplement group were respectively increased and decreased significantly in the placebo group over time (P≤0.05). Supplementation with spirulina could increase plasma antioxidant capacity and reduce oxidative stress in obese men after resistance exercise. Manuscript profile
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        48 - Studying the priming impact with distilled water and salicylic acid on the enzymatic anti-oxidant and the infusion of hemp germination
        شيرين کربلای قلیزاده tooraj mir-mahmoodi نبي  خليلي اقدم
        Salicylic acid has significant role in the different stages of plant development ( phenology ). This molecular messenger plays an active role in the plant's defensive response. To elaborate this, studying morpho-physiologic and biochemical, salicylic acid priming ( SA ) More
        Salicylic acid has significant role in the different stages of plant development ( phenology ). This molecular messenger plays an active role in the plant's defensive response. To elaborate this, studying morpho-physiologic and biochemical, salicylic acid priming ( SA ) impacts in 6 levels ( 500, 750, 1000, 1250, 1500, and 1750 micromullar per litre )together with hydro-priming and control on the hemp seeds in the complete random blocks in 4 replications in the laboratory and greenhouse setting was designed and carried out. The results indicated the meaningful impact of the priming seeds of the hemp, with SA and distilled water on the antioxidant ( CAT, POD ), the significant indexes of germination ( P<0.05 ), and biologic yield of ( P<0.05 ). With the increase of the levels SA, biological yield had ascending process, and in the highest SA level, it showed 43 percent increase in relation to control. Therefore the usage of external SA increased the germination, better establishment of plantlet and improvement of significant indexes in plant physiology specifically in the primary stages of growth which had influence on the biological yield of the plant. Manuscript profile

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