The Effect of High-intensity Interval Training and High-Protein Diet on the Total Oxidant Status and Total Antioxidant Capacity in Cardiac Tissue of Obese Male Rats
Subject Areas :
Journal of Animal Biology
Azin Ghane
1
,
Hossein Abednatanzi
2
,
Marziyeh Saghebjoo
3
,
Mehdi Hedayati
4
1 - Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran
4 - Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Received: 2022-04-04
Accepted : 2022-05-31
Published : 2023-02-20
Keywords:
total antioxidant capacity,
High-intensity interval training,
Heart tissue,
High-protein Diet,
Total Oxidant Status,
Obese Rats,
Abstract :
Obesity causes oxidative stress and is a risk factor for cardiovascular diseases. Exercises and diet modification can affect oxidant and antioxidant factors. The aim of this study was to investigate the effect of high-intensity interval training (HIIT) and high-protein diet (HPD) on total oxidant status (TOS), total antioxidant capacity (TAC) and the ratio of TAC to TOS in the heart tissue of obese male rats. Forty 12-week-old obese male rats (Lee index above 310) were divided into five groups of eight including HIIT (5-12 intervals of 30 seconds with an intensity of 90% VO2max, 5 sessions per week, 10 weeks), HPD (about 57.5% of total calories from protein), HIIT+HPD, obese control-1 (OC-1) and obese control-2 (OC-2) and eight non-obese mice were also divided into the non-obese control (NC) group. became After 48 hours from the last intervention session, heart tissue was removed to measure TAC and TOS levels. Data were analyzed using one-way analysis of variance, post hoc Tukey, Kruskal-Wallis and Mann-Whitney tests at a significance level of p ≤ 0.05. The results showed that the TAC level of heart tissue in the HIIT, HPD, HIIT+HPD groups was significantly higher than in the OC-1 group. On the other hand, the TOS level of heart tissue was significantly lower in the HIIT and HIIT+HPD groups than in the HPD and OC-2 groups. Also, the ratio of TAC to TOS was significantly higher in the HIIT and HIIT+HPD groups compared to the OC-2 group. According to the results, a combination of HIIT and HPD improves TAC and TOS of heart tissue. It seems that HIIT together with HPD strengthens the antioxidant system and reduces oxidative stress in obesity conditions.
References:
Ahmadi N., Farsi S., Azarbayjani M.A. 2019. The effect of high intensity interval training and endurance training on the cAMP gene expression and glycerol in the heart tissue of obese rats. International Journal of Applied Exercise Physiology, 8(1): 159-169.
Alexandre-Santos B., Machado M.V., Menezes A.C., Velasco L.L., Sepúlveda-Fragoso V., Vieira A.B., Frantz E.D.C. 2019. Exercise-induced cardiac opioid system activation attenuates apoptosis pathway in obese rats. Life Sciences, 231: 116542.
Amirkhani Z., Azarbayjani M.A. 2018. Effect of eight weeks resistance training on malondialdehyd, total antioxidant capacity, liver enzyme and lipid profile in overweight and obese women. Journal of Gorgan University of Medical Sciences, 20(3): 48-55. [In Persian]
Attarzadeh Hosseini S.R., Moazzami M., Farahati S., Bahremand M., Sadegh Eghbali F. 2020. Effects of high-intensity interval training versus moderate-intensity continuous training on the total antioxidant capacity, malondialdehyde, and superoxide dismutase in obese/overweight middle-aged women. Iranian Journal of Endocrinology and Metabolism, 22(3): 207-213. [In Persian]
Farhangi N., Nazem F., Zehsaz F. 2017. Effect of endurance exercise on antioxidant enzyme activities and lipid peroxidation in the heart of the streptozotocin-induced diabetic rats. Journal of Shahid Sadoughi University of Medical Sciences, 24(10): 798-809. [In Persian]
Ghane A., Abednatanzi H., Saghebjoo M., Hedayati M. 2022. The effect of high-intensity interval training and high-protein diet on the apoptotic indices of caspase-3, Bax, and Bcl-2 in the cardiac tissue of obese male rats. Daneshvar Medicine, 30(1). [In Persian]
Gibala M.J., Little J.P., MacDonald M.J., Hawley J.A. 2012. Physiological adaptations to low‐volume, high‐intensity interval training in health and disease. The Journal of Physiology, 590(5): 1077-1084.
Johnstone A.M., Horgan G.W., Murison S.D., Bremner D.M., Lobley G.E. 2008. Effects of a high-protein ketogenic diet on hunger, appetite, and weight loss in obese men feeding ad libitum. The American Journal of Clinical Nutrition, 87(1): 44-55.
Kawanishi N., Takagi K., Lee H.C., Nakano D., Okuno T., Yokomizo, T., Machida S. 2018. Endurance exercise training and high-fat diet differentially affect composition of diacylglycerol molecular species in rat skeletal muscle. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 314(6): R892-R901.
Khalafi M., Mohebbi H., Karimi P. 2019. High-intensity interval training increases mitochondria biogenesis in adipose tissue and improves insulin resistance in high fat diet-induced obese rat. International Journal of Applied Exercise Physiology, 8(1): 43-50.
Khanmohammadi R., Azarbaijani M.A, Piri M., Khorsandi L. 2019. The effect of severe periodic training and crocin on oxidative stress in male rats subjected to doxorubicin induction. Armaghane Danesh, 23(6): 694-708. [In Persian]
Lee J.J., Kim H.A., Lee J. 2018. The effects of Brassica juncea L. leaf extract on obesity and lipid profiles of rats fed a high-fat/high-cholesterol diet. Nutrition Research and Practice, 12(4): 298-306.
Martinez-Huenchullan S.F., Ban L.A., Olaya-Agudo L.F., Maharjan B.R., Williams P. F., Tam C.S., Mclennan S.V., Twigg S.M. 2019. Constant-moderate and high-intensity interval training have differential benefits on insulin sensitive tissues in high-fat fed mice. Frontiers in Physiology, 10: 459.
Mohammadi E., Nikseresht F. 2020. Effect of 8 weeks of incremental endurance training on antioxidant enzymes and total antioxidant status of cardiac tissue in experimental diabetic rats. Journal of Shahid Sadoughi University of Medical Sciences, 28(3): 2490-2501. [In Persian]
Parise G., Phillips S.M., Kaczor J.J., Tarnopolsky M.A. 2005. Antioxidant enzyme activity is up-regulated after unilateral resistance exercise training in older adults. Free Radical Biology and Medicine, 39(2): 289-295.
Ropelle E.R., Pauli J.R., Fernandes M.F. A., Rocco S.A., Marin R.M., Morari J., Souza K K., Dias M.M., Gomes-Marcondes M.C., Gontijo J.A.R., Franchini K.G., Velloso L.A., Saad M.J.A., Carvalheira J.B.C. 2008. A central role for neuronal AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) in high-protein diet–induced weight loss. Diabetes, 57(3): 594-605.
Salehi, I., Mohammadi, M., Asadi Fakhr, A. 2009. The effect of treadmill exercise on antioxidant status in the hearts of the diabetic rats. Avicenna Journal of Clinical Medicine, 16(2): 20-27. [In Persian]
Skrzep-Poloczek B., Poloczek J., Chełmecka E., Dulska A., Romuk E., Idzik M., Kazura W., Nabrdalik, Gumprecht J., Jochem, J., Stygar D.M. 2020. The oxidative stress markers in the erythrocytes and heart muscle of obese rats: relate to a high-fat diet but not to DJOS bariatric surgery. Antioxidants, 9(2): 183.
Soori R., Gerami M., Pornemati P., Eskandari A. 2019. Effect of high intensity interval training and continus training on antioxidant enzymes in the heart of the old rats. Journal of Gorgan University of Medical Sciences, 21(2): 26-31. [In Persian]
Tofas T., Draganidis D., Deli C.K., Georgakouli K., Fatouros I. G., Jamurtas A.Z., 2019. Exercise-induced regulation of redox status in cardiovascular diseases: the role of exercise training and detraining. Antioxidants, 9(1): 13.
Villalba J.M., López-Domínguez J.A., Chen Y., Khraiwesh H., González-Reyes J.A., del Río L.F., Gutiérrez-Casado E., del Río M., Calvo-Rubio M., Ariza J., de Cabo R., López-Lluch G., Navas P., Hagopian K., Burón M.I., Ramsey J.J. 2015. The influence of dietary fat source on liver and skeletal muscle mitochondrial modifications and lifespan changes in calorie-restricted mice. Biogerontology, 16(5): 655-670.
Vinke P., Bowen T.S., Boekschoten M.V., Witkamp R.F., Adams V., van Norren K. 2019. Anti-inflammatory nutrition with high protein attenuates cardiac and skeletal muscle alterations in a pulmonary arterial hypertension model. Scientific Reports, 9: 10160.
Wang Y., Chun O.K., Song W.O. 2013. Plasma and dietary antioxidant status as cardiovascular disease risk factors: a review of human studies. Nutrients, 5(8), 2969-3004.
Wycherley T.P., Moran L.J., Clifton P.M., Noakes M., Brinkworth G.D. 2012. Effects of energy-restricted high-protein, low-fat compared with standard-protein, low-fat diets: a meta-analysis of randomized controlled trials. The American Journal of Clinical Nutrition, 96(6): 1281-1298.
Yosefian M., Taghian F., Sharifi G., Hosseini S.A. 2021. Effect of eight weeks of high intensity interval training with and without caloric restriction on oxidative stress in the heart tissue of high-fat diet rats. Journal of Practical Studies of Biosciences in Sport, 9(19): 90-100. [In Persian]
Khorsand Zaker B.S., Saghebjoo M., Islami F. 2022. Effectiveness of high-intensity interval training and high-protein diet on TNF-α protein level in colon tissue of obese male rats: the importance of diet modifying. Obesity Medicine, 31: 100403.
Zarali M., Etemad Z., Azizbeigi K., Karimi P. 2020. Effect of 8 weeks of high intensity interval training (HIIT) with and without calorie restriction on gene expression of caspase-3 and caspase-9 proteins in male rats. Journal of Arak University of Medical Sciences, 23(3), 300-313. [In Persian]
Zhang X., Sergin I., Evans T.D., Jeong S. J., Rodriguez-Velez A., Kapoor D., Chen S., Song E., Holloway K.B., Crowley J.R., Epelman S., Weihl C.C., Diwan A., Fan D., Mittendorfer B., Stitziel N.O., Schilling J.D., Lodhi I.J., Razani B. 2020. High-protein diets increase cardiovascular risk by activating macrophage mTOR to suppress mitophagy. Nature Metabolism, 2: 110-125
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