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Open Access Article
1 - Antibiotic resistance in patients with diabetic foot ulcers
Shima ShantiaeeDiabetes mellitus is a growing problem in today's modern societies. It is difficult to estimate the total number of people suffering from the disease. Approximately 20% of diabetic patients develop wound infections during their life Which in the absence of effective tre MoreDiabetes mellitus is a growing problem in today's modern societies. It is difficult to estimate the total number of people suffering from the disease. Approximately 20% of diabetic patients develop wound infections during their life Which in the absence of effective treatment can disrupt the quality of life of these people. On the other hand, treatment of this complication is very costly. DFIs diabetic foot infections are one of the most important public health issues and the identification of microorganisms that cause microbial infections An antibiotic is good for finding an appropriate treatment. Meanwhile, many reports have shown that antibiotic resistance is rising dramatically. Therefore, early diagnosis of lesions and the rapid onset of antimicrobial treatment are essential for controlling infection and preventing complications and improving the quality of life. An antibiotic susceptibility test is needed to manage infection, which can help in choosing the best treatment options. Manuscript profile -
Open Access Article
2 - Investigating the prevalence of resistance genes in Listeria monocytogenes isolated from food samples
Mohammad Reza Saebi Fahimeh nourbakhsh Hossein Khodabandeh shahrakiListeria monocytogenes (L. monocytogenes) is a type of pathogenic bacteria that causes listeriosis infection. This facultative anaerobic bacterium is able to survive in the presence and absence of oxygen and is the cause of a wide range of diseases in humans and animals MoreListeria monocytogenes (L. monocytogenes) is a type of pathogenic bacteria that causes listeriosis infection. This facultative anaerobic bacterium is able to survive in the presence and absence of oxygen and is the cause of a wide range of diseases in humans and animals. Consumption of contaminated dairy products, meat and vegetables is the most important source of contamination. There are limited studies of the antibiotic resistance of Listeria monocytogenes species. Therefore, this study aims to evaluate the frequency and level of resistance in the evaluated samples. In this descriptive cross-sectional study, 150 different samples were randomly collected from different regions of Isfahan province. The samples included 60 samples of meat, 40 samples of dairy products (including milk, cheese, etc.) and 50 samples of vegetables (including leek, watercress, radish and basil). The serotyping of the isolated strains was done using the commercial O and H antisera of Listeria monocytogenes and according to the manufacturer's instructions, using slide agglutination method and antibiotic resistance evaluation. Standard PCR method was used to detect ermA, ermB, strA, tetS, tetA and ermC genes in the strains. Based on the serological reaction, somatic antigens O and flagella H of Listeria monocytogenes with the corresponding antisera, most Listeria species (70%) belong to serotype 1.2a and the rest from serotype 1.2b (19%) and 4b (11 %) They were. The results of the microbial investigation showed that the highest drug resistance was related to streptomycin (89%) and the lowest drug resistance in the evaluated isolates was related to ampicillin (14%) and chloramphenicol (13%). The most evaluated genes were related to strA gene and ermA gene, with frequencies of 79.8% and 65.4%, respectively. The prevalence of other Listeria monocytogenes genes evaluated in this study included tetA (17%), tetS (2.5%), ermB (10.7%) and ermC (2.1%). Manuscript profile -
Open Access Article
3 - Antibiotic resistance pattern and prevalence of class 1, 2 and 3 integron genes in Escherichia coli strains isolated from urinary tract infections in Shahrekord
Marziyeh Soleymanian نازیلا ارباب سلیمانی sanaz khaksar haghaniIntegrons are mobile genetic elements capable of carrying resistance genes to various antibiotics. These elements have been found in different places of plasmid and chromosome. The aim of this present study was determine the prevalence of class 1, 2 and 3 integrons in E MoreIntegrons are mobile genetic elements capable of carrying resistance genes to various antibiotics. These elements have been found in different places of plasmid and chromosome. The aim of this present study was determine the prevalence of class 1, 2 and 3 integrons in Escherichia coli isolates isolated from urinary tract infection in Shahrekord. In this research, the number of 64 isolates of Escherichia coli were investigated. The antibiotic resistance of the investigated isolates was evaluated using a simple disking method in Mueller Hinton agar medium. In order to determine the frequency of class 1, 2 and 3 integrons, specific primer pairs were used. After the antibiogram test, the highest resistance to ampicillin (75%) and the lowest resistance to imipenem (12.5%) were observed. The frequency of class 1, 2 and 3 integron genes was observed as 12.5%, 6.25% and 3.12%, respectively. None of the integron genes were observed in 52 isolates. In the statistical analysis with chi-square test, a statistically significant relationship was observed between class 1 integron and resistance to the antibiotic ampicillin (p = 0.02 < 0.05). Due to the fact that resistance genes are located on integrons and can be transferred from one strain to another strain and spread resistance in the hospital or other environments, this has doubled the importance of identifying this type of antibiotic resistance genes. Key words: Escherichia coli, integron, antibiotic resistance, urinary infection Manuscript profile -
Open Access Article
4 - Effect of Aerobic and Anaerobic Composting on the Removal of Antibiotics of Enrofloxacin and Ciprofloxacin in Broiler Chicken Manure
mahsa mohammadzadeh forouzan Ghasemian Roudsari Akbar Hassani Abbasali ZamaniBackground and Objective: Many of the antibiotics used in poultry farms are excreted in their feces. This waste is used as organic fertilizer in agriculture and causes the release of animal antibiotics in the environment. These compounds may increase the antibiotic resi MoreBackground and Objective: Many of the antibiotics used in poultry farms are excreted in their feces. This waste is used as organic fertilizer in agriculture and causes the release of animal antibiotics in the environment. These compounds may increase the antibiotic resistance of microbial populations or damage plants grown in these soils. The aim of this study was to determine the effect of aerobic and anaerobic poultry manure composting on the amount of enrofloxacin and ciprofloxacin antibiotics in poultry manure at two levels of high and low concentration and compare them with each other. Method: Two samples of fresh broiler chicken manure were prepared after the end of the chicken treatment with antibiotic enrofloxacin. The two samples contained two levels of high and low concentrations of Enrofloxacin and Ciprofloxacin. The composting of chicken manure was done using a completely randomized design with 4 treatments of 1- aerobic treatments at a low concentration, 2- aerobic treatments at high concentration 3- anaerobic treatments at low concentration 4- anaerobic treatments at a high concentration in five replicates in 75 days. Samples were prepared at specific intervals and the concentration of Enrofloxacin and Ciprofloxacin was measured by HPLC method. The obtained data were fitted with the first-order kinetic equation and the half-life of the antibiotic degradation was calculated. Findings: The results showed that at the end of the period, in treatments 1 to 4, 54.2%, 64.3%, 65.3% and 69.7% of the Enrofloxacin and 41%, 57.8%, 57.8% and 61.8% Ciprofloxacin remained. The correlation coefficient (R2) obtained from the fitting of the first-order kinetic equation in treatments (from 0.71 to 0.92) showed that the data fit well with this equation. The half-life of the Enrofloxacin degradation was obtained between 96.27 and about 150.68 days. The half-life of degradation for Ciprofloxacin was also found to be between 57.28 and 117.48 days. Discussion and Conclusion: Overall, the results showed that the process of composting reduced the concentration of antibiotics and prevented its entry into the environment. The rate and total amount of antibiotic degradation in aerobic conditions was higher than anaerobic. The rate of degradation of both antibiotics at a high initial concentration was lower than its rate of degradation at low concentrations. The rate of ciprofloxacin degradation was also higher than Enrofloxacin. The results of this study can help to create relevant laws and regulations in relevant institutions, in order to reduce the release of veterinary antibiotics into the environment. Manuscript profile -
Open Access Article
5 - Evaluation of the Efficiency of Catalytic Ozonation Process with magnesium-aluminum double layer hydroxide nanocomposite doped on zeolite in mineralization of cefixime antibiotic in Aqueous Solution
yalda sheikh elham Tazikeh-Lemeski yousef Dadban ShahamatBackground and Objective: One of the problems in the health systems of the world today is the prescription or overuse of drugs. Among these, antibiotics are of particular importance. Antibiotics are a group of drugs that are widely used in medicine and veterinary medici MoreBackground and Objective: One of the problems in the health systems of the world today is the prescription or overuse of drugs. Among these, antibiotics are of particular importance. Antibiotics are a group of drugs that are widely used in medicine and veterinary medicine. Cefixime (CFX) is also one of these antibiotics. The aim of this study was to investigate the removal efficiency of cefixime from synthetic sample using catalytic ozonation with Mg-Al layered double hydroxides Doped with zeolite. Material and Methodology: In this experimental and laboratory study MgAl-LDH/Zeolite nanocomposite was used in laboratory reactor and ozonation to remove cefixime. Effect of pH variables (5,6,7,8,9), amount of nanocomposite (0.5,1,1.5,2,2.5 g/L), initial concentration of cefixime (5,10,15,20,25 mg/L) and reaction time (5,18.75,32.5,46.25,60 min) were examined to find the maximum mineralization efficiency and response surface methodology based on central composite design (CCD) was used to design experiments, analyze data and achieve optimal conditions. Analysis of variance was also used to analyze the date. This research was done in 2021-2022. Findings: The maximum mineralization efficiency of cefixime under optimal conditions (pH=8.70, nanocomposite value=1.76 g/L, initial concentration of cefixime=24.06 mg/L, contact time=40.76 min) is 78%, which increases the target efficiency with increasing pH and contact time. Discussion and Conclusion: Catalytic ozonation process with Mg-Al layered double hydroxides nanocomposite Doped with zeolite can be used effectively and efficiency to remove cefixime in aqueous media. Manuscript profile -
Open Access Article
6 - Veterinary Antibiotics, Release in the Environment and its Impact on Soil, Plant and Human Health
Mahsa Mohammadzadeh Forouzan Ghasemian Roudsari Akbar Hassani Abbasali ZamaniBackground and Objective: Veterinary Antibiotics are widely used in Iran and elsewhere in the world to deal with livestock and poultry diseases, due to increased demand for meat and dairy products in the community. Most of these antibiotics are excreted in the urine and MoreBackground and Objective: Veterinary Antibiotics are widely used in Iran and elsewhere in the world to deal with livestock and poultry diseases, due to increased demand for meat and dairy products in the community. Most of these antibiotics are excreted in the urine and feces of livestock and poultry and these excrements are used as manure by farmers in fields and gardens. This issue has raised concerns about the release of antibiotics in the environment. Increasing the concentration of these compounds in agricultural environments may lead to antibiotic-resistant bacteria and genes, and ultimately affect the health of soil, plant, and humans. Method: In this article, a brief overview of the use of antibiotics in the world and Iran, ways to release it in the environment by livestock and poultry manure, its effect on the microbial community of the soil, the accumulation of antibiotics in the edible parts of plants and its impact on the health of human has been studied. Also, the effect of composting livestock and poultry manure before entering the fields and gardens as a suitable strategy to prevent the occurrence of these compounds into the soil has also been studied. Finally, existing research gaps and suggestions for a more accurate understanding of this issue in Iran has been declared. Findings: Most Veterinary antibiotics added to agricultural land are absorbed by plant roots. This leads to their bioaccumulation in plant tissues and toxicity to plants. The type of treatments performed before the introduction of livestock manure into the field determines the final concentration of antibiotics introduced into the environment. Discussion and Conclusions: There are significant research gaps in the release of antibiotics into the environment. In Iran, the concentration of various antibiotics in manure and poultry as well as their fate in soil, plants and humans should be investigated. Key words: Veterinary antibiotics (VAs), Agricultural soil, Manure fertilizer, Antibiotic resistance. Manuscript profile -
Open Access Article
7 - Isolation and identification of gram-negative bacteria from patients with urinary tract infections referred to Savadkooh laboratory and determination of their drug resistance pattern from the beginning of October 1400 to the end of December 1400
seyedmohammadmahdi mousavi mojtaba fallah neda Jafari JushqhanToday, microorganisms have become resistant to drugs by causing genetic mutations. The aim of this study was to determine the antibiotic resistance of gram-negative bacteria isolated from urine culture in the laboratory of Savadkuh city. The cross-sectional descriptive MoreToday, microorganisms have become resistant to drugs by causing genetic mutations. The aim of this study was to determine the antibiotic resistance of gram-negative bacteria isolated from urine culture in the laboratory of Savadkuh city. The cross-sectional descriptive study was performed on gram-negative bacteria isolated from urinary tract infections in Savadkuh laboratory. Bacterial isolates were identified by microscopic examination, morphology and biochemical tests. Fifty samples of gram-negative bacteria causing urinary tract infections were isolated from 5 cases (10%) of men and 45 cases (90%) of women. According to the study, the two most common gram-negative bacteria in urinary tract infections are based on the percentage of infectivity in this study, which include: E.Coli (62%), and Klebsiella (30%) and other gram-negative (8%), They were isolated from urine samples. According to the antibiogram test, the most cases of resistance were related to the antibiotics ampicillin, nalidixic acid and cefazolin, respectively. On the other hand, the most sensitive drugs are nitrofurantoin, sulfamethoxazole trimethoprim and ciprofloxacin, respectively. Manuscript profile -
Open Access Article
8 - Development of liposomal formulations carrying antibiotics
rabea khoshnevis zadehSince the advent of antibiotics, selective pressure on microorganisms has caused the formation of resistant types. Different generations of antibiotics have been designed and used to fight pathogens, but at the same time, more resistant types of pathogens have been spre MoreSince the advent of antibiotics, selective pressure on microorganisms has caused the formation of resistant types. Different generations of antibiotics have been designed and used to fight pathogens, but at the same time, more resistant types of pathogens have been spread, so it is necessary to use new strategies to deal with infectious agents. The development of nanoscience in the field of medicine was a suitable solution for this purpose, and pharmaceutical nanocarriers were developed. Liposomes are nanocarriers with a lipid body whose features such as biocompatibility, bioavailability, targeted transfer, amphiphilic properties, integration with membranes, slow and controlled drug release have attracted the attention of researchers. In this review article, an attempt has been made to provide information about the benefits of this drug system by studying scientific documents in the field of antibiotic-carrying liposomes. Manuscript profile -
Open Access Article
9 - Antibiotics in the environment: An overview of the sources, distribution, occurrence, effects, fate and risk assessment of antibiotics in the environment
Gholamhossein Safari Ahmad Asl hashemi Nasim MousakhaniAntibiotics are one of the largest groups of medicinal compounds that are used in medicine and veterinary medicine. These compounds, despite having positive effects in preventing and treating diseases in humans and animals, also have adverse effects on the environment. MoreAntibiotics are one of the largest groups of medicinal compounds that are used in medicine and veterinary medicine. These compounds, despite having positive effects in preventing and treating diseases in humans and animals, also have adverse effects on the environment. Recently, the indiscriminate use of antibiotics and the subsequent entry of these compounds into the environment have led to an increase in concerns at the global level These compounds are not traditionally considered environmental pollutants, however, there are concerns about the occurrence of these compounds in aquatic systems, due to their biological effects and potential risks to the environment and health human.. Conventional water and wastewater treatment processes are not designed to remove small amounts of chemicals such as antibiotics. Therefore, the entry of these compounds into the environment from different sources leads to the pollution of plants, animals, and water and soil environments and also creates problems for public health. In addition, the presence of these medicinal compounds in the environment leads to the development of antibiotic-resistant pathogens that potentially threaten the functioning of the ecosystem and human health. Therefore, it is necessary to prevent the indiscriminate entry of these compounds into the environment and to provide effective and efficient wastewater treatment systems in the production sources of these compounds in order to eliminate and minimize their environmental risks. Manuscript profile -
Open Access Article
10 - Evaluation of chloramphenicol residues in poultry meat using ELISA method in Isfahan
ابراهیم Rahimi محسن JafarianChloramphenicol is classified as a broad spectrum veterinary drugs used to treat pulmonary infections in the poultry industry. Even relatively low levels of chloramphenicol residues may give rice to an irreversible type of bone marrow depression leading eventually to an MoreChloramphenicol is classified as a broad spectrum veterinary drugs used to treat pulmonary infections in the poultry industry. Even relatively low levels of chloramphenicol residues may give rice to an irreversible type of bone marrow depression leading eventually to anaplastic anemia. This study was conducted with the aim of chicken meat. Chloramphenicol concentraction was measured by enzyme (ELISA) in 140 chicken meat samples (thigh muscles) presented to the consumption market of isfahan city. In 25 of the 140 evaluated samples (17.9%), presence of chloramephnicol residues was detected in concentrations rouging form 14 to 311 ng/kg with a mean ±standard deviation of 97.9 ±17.7 ng/kg. the results of chloramphenicol residues were low in the samples but this drug is still being used in the poultry industry and this can be a risk to public health. Manuscript profile -
Open Access Article
11 - The comparative effect of prebiotic, growth promoter antibiotic, probiotic, yeast cell wall and acid fire on broiler chickens performance
افشین Zakeri مهدی Taghi Nejad Roudbaneh آیدین Azizpour وحید HajiabalooIn this study 600 Cobb 500 broiler chickens were divided in six similar groups (A, B, C, D, E&F) with 100 chickens in each group (with four replicates of 25 chickens in each group). 100 g/ton growth promoter antibiotic in experimental group (B), 100 g/ton Prebiotic MoreIn this study 600 Cobb 500 broiler chickens were divided in six similar groups (A, B, C, D, E&F) with 100 chickens in each group (with four replicates of 25 chickens in each group). 100 g/ton growth promoter antibiotic in experimental group (B), 100 g/ton Prebiotic in experimental group (C), 800 g/ton Acid fires in experimental group (D), 1 kg/ton MOS in experimental group (E) and 4 kg/ton Yeast cell wall in experimental group (F) were added to the basic diets however the control group (A) chickens were fed only with the basic diet. Every week 100 chickens from each group were selected randomly and productive parameters such as weight gain, FCR, EEF, mortality, feed intake (FI) were calculated. On days 9, 17 and 25 of growth (1 day before and 7, 14 days after first Newcastle B1 vaccination), from each groups, each time 20 chickens were chosen randomly and serum antibody titres of these chickens were measured against Newcastle vaccine by HI test. The results obtained from statistical analysis indicated that using natural growth promoting substances not only increases humoral immunity but it also leads to improvement of productive parameters (p<0.05). Manuscript profile -
Open Access Article
12 - Synthesis and evaluation of antibacterial properties of green copper oxide nanoparticles from Hypericum perforatum plant extract and Marrubium Vulgare
Ashkan Farazin Shirin KavezadehIn the present study, copper oxide nanoparticles were synthesized using Hypericum perforatum plant extract from the Malpican family and Marrubium Vulgare plant from the mint family. Since it is believed that the antioxidants in the plant reduce the reduction of metal io MoreIn the present study, copper oxide nanoparticles were synthesized using Hypericum perforatum plant extract from the Malpican family and Marrubium Vulgare plant from the mint family. Since it is believed that the antioxidants in the plant reduce the reduction of metal ions to nanoparticles as reducing agents, these two plants were tested for their antioxidant properties by the free radical scavenging method, and the IC50 quantity was measured. Hypericum perforatum plant with IC50 equivalent to 0.413 had more antioxidant content than Marrubium Vulgare plant with IC50 equivalent to 1.562, so it was superior in the process of green synthesis. The properties of the synthesized nanoparticles were analyzed using X-ray diffraction (XRD), Scanning electron microscope (SEM), and Ultraviolet-visible (UV-Visible). The presence of a metal-oxygen bond was confirmed by Fourier-transform infrared spectroscopy (FTIR). X-ray energy diffraction spectra showed the purity of the synthesized nanoparticles. The synthesized nanoparticles were observed with spherical morphology and size distribution of 30 to 40 nm and with uniform size distribution. The results of the XRD spectrum showed that pH adjustment did not affect the synthesis of copper oxide nanoparticles. The nanoparticles synthesized against the two bacteria used in this present study did not show significant antibacterial properties compared to industrial antibiotics. Manuscript profile -
Open Access Article
13 - Evaluation of Medicinal infusions signs in cattle slaughtered at the abattoir Sari
mohsen yadollahi Nasrollah Vahedi Nouri Yazdan KhodabakhshSummary: Today, Antibiotics are mainly used to treat and also stimulate the growth of livestock in the diet. In the event of uncontrolled consumption of drugs especially antibiotics and lack of observance Contraception time (Withdrawal time), Causes harmful effects such MoreSummary: Today, Antibiotics are mainly used to treat and also stimulate the growth of livestock in the diet. In the event of uncontrolled consumption of drugs especially antibiotics and lack of observance Contraception time (Withdrawal time), Causes harmful effects such as: Bacterial resistance, allergic reactions, poisoning, Carcinogenicity and disturb the natural microflora of the intestines in the body of the consumer. This study was conducted to determine the rate of injection of drugs and its effects on cows' carcasses. This study is descriptive-cross sectional. Within six months, From June to December 2016, a total of 8630 cow carcasses were surveyed from Sari industrial slaughterhouse. For this purpose, carcasses were examined (Effects of Injection, Smell the drug odor) daily at the slaughterhouse. Of the 8630 carcasses examined, 1,500 carcasses (17.38 %) had injectable effects. The results of the research show that there the difference between males and females in terms of the effects of drug injections is statistically significant in both summer and autumn (P‹0.05).But between summer and autumn, the difference is not statistically significant(P›0.05). Manuscript profile -
Open Access Article
14 - The study of Escherichia coli contamination rate in meats of chickens without antibiotic and conventional
saeed Dehghani Majid Gholami-Ahangaran Ebrahim RahimiThe residue of antibiotics and other chemical compounds in chicken meat is one of main concerns in consumers of this protein source. In this research, for study of correlation between antibiotic consumption and chicken meat contamination, in summer of 2014, 10 chicken f MoreThe residue of antibiotics and other chemical compounds in chicken meat is one of main concerns in consumers of this protein source. In this research, for study of correlation between antibiotic consumption and chicken meat contamination, in summer of 2014, 10 chicken farms in Isfahan province that reared under conventional condition and 5 chicken farms that reared broilers under without antibiotic consumption were monitored from start of growing period. At end of growing period, in slaughter stage, one piece of breast muscle and cecum content were sampled. The percentage of carcass contamination and number of Escherichia coli (E.coli) in cecal content were determined in chickens reared under conventional and without antibiotic condition. Also, the shigatoxogenic E.coli was examined by PCR.Furthermore, the carcass contamination and E.coli colony count in cecal content in chickens with or without colibacillosis background were determined. The results showed that the chicken meat contamination to E.coli and the E.coli population in cecal content in chickens reared under conventional condition were not significant different from chickens reared without antibiotic, while these indices in chickens with colibacillosis history were significant higher than chickens without colibacillosis history. In this study the shigatoxogenic E.coli was not detected. In overall, this study revealed microbial infection in chicken farms that lead to antibiotic consumption could decrease hygienic quality of chicken meat. Therefore, in addition to controlling of withdrawal time for antibiotic consumption, the withdrawal time for infections incidence must be observed for decrease the risk of transmission of pathogens along the food chain of human. Manuscript profile -
Open Access Article
15 - The comparing of antibiotic resistance pattern in Escherichia coli isolates from chicken meat that reared under conventional and without antibiotic condition
Mohammadreza Shahiri Majid Gholami-Ahangaran Ebrahim RahimiThe using of antibiotics in chicken production is one of main concerns in consumers of this protein source that usually these compounds administrated for antimicrobial effect and control of microbial infections in poultry production. For comparing the antibiotic resista MoreThe using of antibiotics in chicken production is one of main concerns in consumers of this protein source that usually these compounds administrated for antimicrobial effect and control of microbial infections in poultry production. For comparing the antibiotic resistance pattern in Escherichia coli (E. coli) that isolated from different growing conditions (conventional and without antibiotic), the E. coli strains were isolated from chicken meat and cecal content of chickens that were reared under conventional and without antibiotic conditions, in Isfahan province. After purification of E.coli strains on specific bacterial culture, and approve of bacterial strains according to biochemical tests, the isolates were cultured on Mueller-Hinton culture. The antibiotic resistance pattern was examined by disc-diffusion agar test. The results showed that the antibiotic resistance to different medicinal and veterinary antibiotics groups has occurred in two different growing conditions but the antibiotic resistance was lower in without antibiotic than conventional growing condition. Manuscript profile -
Open Access Article
16 - Antibiotic resistance and presence of integron class 1 and class 2 genes amongst Escherichia coli isolates of urine specimens of inpatients and outpatients in Ahvaz, southern of Iran
Abbas Farahani Mahsa Dastranj Jebreil Shamseddin Hojat Veisi Saber Soltani Hadi KalantarIntegrons play an essential role in spreading antibiotic resistance genes among Escherichia coli isolates. The aim of this study was to determine the prevalence of class 1 and 2 integrons amongst Escherichia coli isolates producing broad-spectrum beta-lactamases (ESBLs) MoreIntegrons play an essential role in spreading antibiotic resistance genes among Escherichia coli isolates. The aim of this study was to determine the prevalence of class 1 and 2 integrons amongst Escherichia coli isolates producing broad-spectrum beta-lactamases (ESBLs) in patients with urinary tract infections referred to Ahvaz teaching Hospital in 2017-2018. In this cross-sectional descriptive study, isolates were determined using conventional methods. Antibiotic susceptibility was measured by agar disc diffusion method. Production of ESBLs enzymes was measured via double disc method. Finally, presence of genes related to class 1 and 2 integrons was done using specific primers and Polymerase chain reaction method. Amongst 123 Escherichia coli ESBLs producing isolates the highest resistance was related to Cefotaxime, Ceftazidime, Cotrimoxazole and, Nalidixic acid, respectively, and the least resistance was related to Imipenem. About 84 (68.29%) isolates had multiple drug resistance (MDR). Additionally, 93 (75.60 %) isolates had class 1 integron and 11 (8.94 %) isolates had class 2 integron. There were no significant relationships between the presence of integrons and resistance to different antibiotics (p > 0.05). High prevalence of class 1 integron amongst Escherichia coli isolates producing broad-spectrum β-lactamase may contribute to resistance to common antibiotics. Therefore, identifying frequency of integrons and their relationship with drug resistance patterns seems to be necessary. Manuscript profile -
Open Access Article
17 - Investigating the performance, intestinal microbiology and blood parameters, following the addition of probiotic, and antibiotic to the diet of Arian breed broilers
Farshad Najjar Asiabani Mojtaba Bazaei Mohammad Pouranian Seyed Ali Raeissadat S BelivandIndiscriminate use of antibiotics causes the increase of antibiotic resistance and the transfer of antibiotic resistant genes from animals to humans, so it is necessary to find suitable alternatives for antibiotics to minimize these factors, which the present experiment MoreIndiscriminate use of antibiotics causes the increase of antibiotic resistance and the transfer of antibiotic resistant genes from animals to humans, so it is necessary to find suitable alternatives for antibiotics to minimize these factors, which the present experiment pursued the same goal. 420 one-day-old broiler chickens were used for 42 days in the form of a completely randomized design with 7 treatments, 3 replications and 20 chickens per replication. The experimental treatments included the control diet and three levels of protexin and virginiamycin 10% (0.25, 0.5, and 0.75). At the end of the data analysis, GLM method was used to compare the averages with Duncan's multi-range test. In the entire breeding period, the amount of feed consumed under the influence of experimental treatments containing protoexin and virginiamycin decreased compared to the control (p<0.5). The effect of protexin on live weight, ready-to-cook carcass and average daily weight gain was significant, so that in the treatments containing protexin, the live weight and ready-to-cook carcass were higher than the control (p<0.5). The treatment containing 0.5% of protexin had the highest live weight and ready-to-cook carcass weight (p<0.5). The concentration of blood parameters of glucose, cholesterol, triglycerides, high, low and very low density lipoproteins and blood urea nitrogen were affected was higher than the control (p<0.5). Therefore, the results of the present study showed that the effect of probiotics is not only equal to antibiotics, but also has better and more useful effects in some cases, so it is possible to use probiotics instead of antibiotics. He suggested virginiamycin in the diet. Manuscript profile -
Open Access Article
18 - Studying the therapeutic effect of Oliveria decumbens vent. plant essential oil in the treatment of bladder inflammation caused by Escherichia coli in male Wistar rats
Hossein Antikchi S Mashhady Rafie Negar Panahi کیومرث AminiAntibiotic resistance, especially in Escherichia coli, is one of the most worrying issues worldwide. In addition, the side effects of chemical drugs have led scientists to use natural alternatives with better therapeutic effects and fewer side effects than common treatm MoreAntibiotic resistance, especially in Escherichia coli, is one of the most worrying issues worldwide. In addition, the side effects of chemical drugs have led scientists to use natural alternatives with better therapeutic effects and fewer side effects than common treatments. Therefore, this research has studied the therapeutic effect of Oliveria decumbens plant essential oil in the treatment of inflammation in the bladder caused by Escherichia coli infection in male Wistar rats. The antibacterial properties of the plant extract were investigated using the standard microdilution method against Escherichia coli. Then, to cause cystitis, Escherichia coli bacteria with a concentration of 1.5 x 108 CFU/mL was injected into the bladder of mice, and then different concentrations of the extract and gentamicin were administered to them. Bladder histopathological parameters were evaluated at the end of the study. MIC and MBC of the extract against Escherichia coli were 0.32 μl/ml and 512 μl/ml, respectively. In the infected group without treatment, the infiltration of inflammatory cells and the thickness of the bladder tissue increased significantly, but in the extract-treated groups, especially at higher doses, these parameters decreased significantly (p<0.01). The effect of the extract in reducing the number of bacteria was comparable to gentamicin. In addition, after administration of the extract, inflammation, fibrosis and thickness of the epithelium also decreased in a dose-dependent manner (p<0.01). The results of this research showed that the treatment with the extract of this plant, which was prescribed as an oral supplement or subcutaneous injection, led to a significant decrease in the number of urinary bacteria and improved pathological changes in the studied mice. Manuscript profile -
Open Access Article
19 - Investigate the frequency of genes sul and investigate the relationship between genes sul and Class I integrons in Klebsiella pneumoniae isolates resistant to sulfonamides isolated from clinical cases in Shahrekord
Marziyeh Farsinejad Maryam Reisi JAMSHID Alibabaeishahraki Hossein Khodabandeh shahrakiAntimicrobial resistance is a common problem throughout the world. Acquire integrons is one One of the main causes multi- resistance in gram-negative microorganisms. The purpose of this study, Investigate the frequency of genes sul and investigate the relationship betw MoreAntimicrobial resistance is a common problem throughout the world. Acquire integrons is one One of the main causes multi- resistance in gram-negative microorganisms. The purpose of this study, Investigate the frequency of genes sul and investigate the relationship between genes sul and Class I integrons in Klebsiella pneumoniae isolates resistant to sulfonamides isolated from clinical cases in Shahrekord. In this study, antibiotic resistance 90 isolates of Klebsiella pneumoniae isolated from clinical cases of Shahrekord,disk diffusion method was performed. In order to investigate resistance to sulfonamides of the antibiotic cotrimoxazole was used. Then using specific primers was performed tracing genes sul1, sul2 and sul3 and intI. After PCR reaction of 33 isolates resistant to trimethoprim-sulfamethoxazole sul1 gene in 15 isolates (45/45%), sul2 gene in 20 isolates (60/60%), gene Sul3 in 2 isolates (6/06%) and the gene intI 27 isolates was found. The statistical analysis between genes sul1and IntI significant relationship was observed. The results of this study show that is a strong correlation between carry integrons and increased resistance to a number of different classes of antibiotics. Manuscript profile
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