Development of efficient analysis method for determination of extensively used tetracycline antibiotics in egg samples

Subject Areas : Veterinary Clinical Pathology

Neda Mohafez ¹ , Afshin Javadi ² , Mohammad Reza Afshar Mogaddam ³ , Zohreh Mashak ⁴

1 - Ph.D. Student, Department of Food Science and Technology, Mamaghan Branch, Islamic Azad University, Mamaghan, Iran.
2 - Professor, Department of Food Hygiene, Faculty of Veterinary, Tabriz Medical Science, Islamic Azad University, Tabriz, Iran.
3 - Assistant Professor, Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 - Associate Professor, Department of Food Hygiene, Karaj Branch, Islamic Azad University, Karaj, Iran.

Received: 2024-05-05 Accepted : 2024-09-01 Published : 2024-11-20

Keywords: DSPE, MOF, Tetracycline antibiotics, HPLC, Egg,

Abstract :

Antibiotics are chemical compounds that nowadays are widely used to treat diseases in animals all over the world. Ignorance or negligence of withdrawal time or using antibiotics more than prescribed amount has caused the presence of their residues in foodstuffs like egg samples that can have adverse effects on consumer health. Therefore, monitoring the presence of antibiotics in various foodstuffs especially in highly consumed products like egg is of great importance. The aim of this sudy is Development of efficient analysis method for determination of extensively used tetracycline antibiotics in egg samples. So, In the present research, a dispersive solid phase extraction combined with dispersive liquid-liquid microextraction is proposed for the extraction of tetracycline antibiotics (oxytetracycline, tetracycline, doxycycline, and chlortetracycline) from egg samples. The extracted analytes are analyzed using high-performance liquid chromatography equipped with a diode array detector. Under the optimum condition, limits of detections and quantifications within the ranges of 0.16–0.25 and 0.54–0.83 ng g–1 were obtained, respectively. Extraction recoveries has been obtained in the range of 65–82%, respectively. Oxytetracycline was found in a number of samples (six out of fifteen investigated samples). Also, according to the obtained results, ultraviolet irradiation can reduce the residue amount of oxytetracycline significantly.

References:

• Almeida, C., Fernandes, J.O. and Cunha, S.C. (2012). A novel dispersive liquid–liquid microextraction (DLLME) gas chromatography-mass spectrometry (GC–MS) method for the determination of eighteen biogenic amines in beer. Food Control, 25(1): 380-388.
• Asgharinezhad, A.A., Ebrahimzadeh, H., Mirbabaei, F., Mollazadeh, N. and Shekari, N. (2014). Dispersive micro-solid-phase extraction of benzodiazepines from biological fluids based on polyaniline/magnetic nanoparticles composite. Analytica Chimica Acta, 24: 844:80-89.
• Babapour, A., Azami, L. and Fartashmehr, J. (2012). Overview of antibiotic residues in beef and mutton in ardebil, north west of Iran. World Applied Sciences Journal, 19(10): 1417-1422.
• Baghaei, P.A.M., Afshar Mogaddam, M.R., Farajzadeh, M.A., Mohebbi, A. and Sorouraddin, S.M. (2022). Application of deep eutectic solvent functionalized cobalt ferrite nanoparticles in dispersive micro solid phase extraction of some heavy metals from aqueous samples prior to ICP-OES. Journal of Food Composition and Analysis, 117: 105-125.
• Birmpa, A., Vantarakis, A., Paparrodopoulos, S., Whyte, P. and Lyng, J. (2014). Efficacy of three light technologies for reducing microbial populations in liquid suspensions. BioMed Research International, 1: 1-9.
• Du, F., Sun, L., Tan, W., Wei, Z., Nie, H., Huang, Z., et al. (2019). Magnetic stir cake sorptive extraction of trace tetracycline antibiotics in food samples: preparation of metal–organic framework–embedded poly HIPE monolithic composites, validation and application. Analytical and Bioanalytical Chemistry, 411(10): 2239-2248.
• Gissawong, N., Boonchiangma, S., Mukdasai, S. and Srijaranai, S. (2019). Vesicular supramolecular solvent–based microextraction followed by high performance liquid chromatographic analysis of tetracyclines. Talanta, 200: 203-211.
• Huang, L., Yu, W., Guo, X., Huang, Y., Zhou, Q. and Zhai, H. (2019). Chip-¬based multi--molecularly imprinted monolithic capillary array columns coated Fe3O4/GO for selective extraction and simultaneous determination of tetracycline, chlortetracycline and deoxytetracycline in eggs. Microchemical Journal.150: 104097.
• Menkem, Z.E., Ngangom, B.L., Tamunjoh, S.S.A. and Boyom, F.F. (2019). Antibiotic residues in food animals: Public health concern. Acta Ecologica Sinica, 39(5): 411-415.
• Mohebbi, A., Nemati, M., Farajzadeh, M.A., Afshar Mogaddam, M.R. and Lotfipour, F. (2022). High performance liquid chromatography–tandem mass spectrometry determination of patulin and ochratoxin a in commercial fruit juices after their extraction with a green synthesized metal organic framework–based dispersive micro solid phase extraction procedure. Microchemical Journal, 179: 107558.
• Mohebbi, A., Nemati, M., Mogaddam, M.R.A., Farajzadeh, M.A. and Lotfipour, F. (2022). Dispersive micro–solid–phase extraction of aflatoxins from commercial soymilk samples using a green vitamin–based metal–organic framework as an efficient sorbent followed by high performance liquid chromatography–tandem mass spectrometry determination. Journal of Chromatography A, 1673: 463099.
• Moudgil, P., Bedi, J.S., Aulakh, R.S., Gill, J.P.S. and Kumar, A. (2019). Validation of HPLC multi–residue method for determination of fluoroquinolones, tetracycline, sulphonamides and chloramphenicol residues in bovine milk. Food Analytical Methods, 12: 338-346.
• Nascimento, U.M. and Azevedo, E.B. (2013). Microwaves and their coupling to advanced oxidation processes: Enhanced performance in pollutants degradation. Journal of Environmental Science and Health (Part A), 48(9): 1056-1072.
• Oka H., Ito, Y. and Matsumoto, H. (2000). Chromatographic analysis of tetracycline antibiotics in foods. Journal of Chromatography A, 882(1-2): 109-133.
• Pang, Y.H., Lv, Z.Y., Sun, J.C., Yang, C. and Shen X.F. (2021). Collaborative compounding of metal-organic frameworks for dispersive solid-phase extraction HPLC–MS/MS determination of tetracyclines in honey. Food Chemistry, 355: 129411.
• Rahmani, A., Mehralipour, J., Shabanlo, A. and Majidi, S. (2015). Efficiency of ciprofloxacin removal by ozonation process with calcium peroxide from aqueous solutions. The Journal of Qazvin University of Medical Sciences, 19(2): 55-64. [In Persian]
• Ścigalski, P. and Kosobucki, P. (2020). Recent materials developed for dispersive solid phase extraction. Molecule, 25(21): 4869.
• Seifrtová, M., Nováková, L., Lino, C., Pena, A. and Solich, P. (2009). An overview of analytical methodologies for the determination of antibiotics in environmental waters. Analytica Chimica Acta, 649(2): 158-179.
• Sereshti, H., Semnani Jazani, S., Nouri N. and Shams, G. (2020). Dispersive liquid-liquid microextraction based on hydrophobic deep eutectic solvents: Application for tetracyclines monitoring in milk. Microchemical Journal, 158(7): 105269.
• Simon, R.R., Borzelleca, J.F., DeLuca, H.F. and Weaver, C.M. (2013). Safety assessment of the post-harvest treatment of button Mushrooms (Agaricus bisporus) using ultraviolet light. Food and Chemical Toxicology, 56(4): 278-289.
• Wang, K., Lin, K., Huang, X. and Chen, M. (2017). A simple and fast extraction method for the determination of multiclass antibiotics in eggs using LC-MS/MS. Journal of Agricultural and Food Chemistry, 65(24): 5064-5073.
• Wu, X., Hong, H., Liu, X., Guan, W., Meng, L., Ye, Y., et al. (2013). Graphene-dispersive solid-phase extraction of phthalate acid esters from environmental water. Science of the Total Environment, 444: 224-230.
• Wu, Q., Wang, C., Liu, Z., Wu, C., Zeng, X., Wen, J., et al. (2009). Dispersive solid-phase extraction followed by dispersive liquid-liquid microextraction for the determination of some sulfonylurea herbicides in soil by high-performance liquid chromatography. Journal of Chromatography A, 1216(29): 5504-5510.
• Yang, X., Zhang, S., Yu, W., Liu, Z., Lei, L., Li, N., et al. (2014). Ionic liquid-anionic surfactant based aqueous two-phase extraction for determination of antibiotics in honey by high-performance liquid chromatography. Talanta, 124: 1-6.