Development of efficient analysis method for determination of extensively used tetracycline antibiotics in egg samples
Neda Mohafez
1
(
Department of Food Science and Technology, Mamaghan Branch, Islamic Azad University, Mamaghan, Iran
)
Afshin Javadi
2
(
Department of Food Hygiene, Veterinary Faculty, Islamic Azad University of Tabriz, Tabriz, Iran.
)
Mohammad Reza Afshar Mogaddam
3
(
Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
)
Zohreh Mashak
4
(
Department of Food Hygiene, Karaj Branch, Islamic Azad University, Karaj, Iran
)
Keywords: DSPE, MOF, Tetracycline antibiotics, HPLC, Egg,
Abstract :
Antibiotics are chemical compounds that nowadays are widely used to treat diseases in animals all over the world. Ignorance or negligence of withdrawal time or using antibiotics more than prescribed amount has caused the presence of their residues in foodstuffs like egg samples that can have adverse effects on consumer health. Therefore, monitoring the presence of antibiotics in various foodstuffs especially in highly consumed products like egg is of great importance. The aim of this sudy is Development of efficient analysis method for determination of extensively used tetracycline antibiotics in egg samples. So, In the present research, a dispersive solid phase extraction combined with dispersive liquid-liquid microextraction is proposed for the extraction of tetracycline antibiotics (oxytetracycline, tetracycline, doxycycline, and chlortetracycline) from egg samples. The extracted analytes are analyzed using high-performance liquid chromatography equipped with a diode array detector. Under the optimum condition, limits of detections and quantifications within the ranges of 0.16–0.25 and 0.54–0.83 ng g–1 were obtained, respectively. Extraction recoveries has been obtained in the range of 65–82%, respectively. Oxytetracycline was found in a number of samples (six out of fifteen investigated samples). Also, according to the obtained results, ultraviolet irradiation can reduce the residue amount of oxytetracycline significantly.
