Quantification of Antibiotic Residues in Local and Imported Broiler Chicken Meat in Al-Batina Governorate using Liquid Chromatography-Mass Spectrometry

Subject Areas : Journal of Chemical Health Risks

Hiba Hamood Saif AL Kiyumi ¹ , Lama Ali Salim AL Manai ² , Dr Ahmed Abusham ³ , Salem Said Jaroof Al Touby ⁴ , Waleed Khalid Hilal Al Rajhi ⁵ , Yahya Bin Abdullah Alrashdi ⁶ , Md. Amzad Hossain ⁷

1 - Nizwa University
2 - Nizwa University, Oman
3 - Nizwa University
4 - Nizwa UNiversity
5 - Nizwa University
6 - Nizwa University
7 - School of Pharmacy, College of Health Sciences, University of Nizwa, P. O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman

Received: 2023-12-05 Accepted : 2024-09-20 Published : 2024-09-24

Keywords: Omani broiler chickens, Imported broiler chickens, Extraction, Antibiotics, MS/Q-TOF,

Abstract :

Antibiotics are widely used in poultry to increase meat production and prevent infections. The aim of this study is to quantitative and qualitative determination of antibiotic residue in the chicken samples. The antibiotic residues were extracted from the local and imported broiler chicken samples by applying modified established methods and quantified them by liquid chromatography-mass spectrometry (MS/Q-TOF). Both antibiotics were extracted from the meat and kidney collected from chicken using well-established methods. The extracted samples were analyzed by using the sensitive MS/Q-TOF. The experimental results showed that collected local and imported chicken samples contain five analyzed antibiotics and are within the permissible limit except for gentamicin. In the imported meat sample, the highest amount of antibiotics was, sulfanilamide, and the lowest was levofloxacin. However, in the local breast meat samples, among the analyzed antibiotics, the amount of gentamicin in both local chicken collected from Al Safwa and Waeel is too high, 0.202 m kg-1 and 369.87, respectively compared to the maximum residue level (MRL). Other antibiotics in the breast meat samples are within the MRL values. In kidney samples in the local broiler chicken, the amount of all analyzed antibiotics was within the MRL values. The highest concentration was sulfanilamide, followed by gentamicin > oxytetracycline > chloramphenicol > levofloxacin. In conclusion, all the imported and local broiler meat and kidney contains the five analyzed antibiotics within the MRL values, except gentamicin was available in high amounts in the local breast meat. In conclusion, the excessive amount of gentamicin in the chicken samples through diet will create significant health troubles.

References:

1. Mathew H., Andrew T., Barrie W., 2019. Antibiotics: past, present, future. Gaming Law Review Economist. 51, 72-80.
2. Mehdi Y., Létourneau-Montminy M.P., Gaucher M.L., Chorfi Y., Suresh G., Rouissi T., 2018. Use of antibiotics in broiler production: global impacts and alternatives. Animal Nutritive. 4, 170–8.
3. Youcef M., 2018. Antibiotics are used to fight bacterial infections. However the use of antibiotics in broiler production: Global impacts and alternatives, Animal Nutrition. Elsevier. Available at: https://www. sciencedirect.com/science /article /pii/S2405654517302512, visited April 13, 2023.
4. Roth N., Käsbohrer A., Mayrhofer S., Zitz U., Hofacre C., Domig K.J., 2019.The application of antibiotics in broiler production and the resulting antibiotic resistance in Escherichia coli: A global overview. Poultry Sciences. 94(4), 1791-1804.
5. Al-Qamashoui B., 2014. Towards conservation of Omani local chicken: Phenotypic characteristics, management practices and performance traits, Asian-Australasian journal of animal sciences. U.S. National Library of Medicine. Available at: https://www.ncbi.nlm.nih.gov/pmc/ articles/PMC4093168/visited April 10, 2023.
6. Times of Oman, 2022. Governorates /topics/ Agriculture/Trade-Import-Quantity/Meat-imports-quantity, visited September 29, 2022.
7. Günsel A., Taslimi P., Atmaca G.Y., Bilgicli A.T., Pişkin H., Ceylan Y., Erdoğmuş A., Yarasir M.N., Gülçin İ., 2021. Novel potential metabolic enzymes inhibitor, photosensitizer and antibacterial agents based on water-soluble phthalocyanine bearing imidazole derivative. Journal of Molecular Structure. 1237,130402.
8. Randall S., Singer Charles, Hofacra, 2006. The potential of antibiotic use in poultry production. Avian Diseases. 50(2), 161-172
9. Liousia M., Gousia P., Conomou N., Sakkas H., Papadopoulou C., 2015. Screening for antibiotic residues in swine and poultry tissues using the STAR test. International Journal Food Safety Nutrition Public Health. 5(2), 173-188.
10. Ralph J., Rich Ara H., 1981. A new screening method for the detection of antibiotic residues in meat and poultry tissues. Journal of Food Protection. 44 (11), 828-831.
11. Kabir J., Umoh V., Audu-okoh E., Umoh J., Kwaga J., 2004. Veterinary drug use in poultry farms and determination of antimicrobial drug residues in commercial eggs and slaughtered chicken in Kaduna State, Nigeria. Food Contamination. 15(2), 99-105.
12. Baazize-Ammi D., 2020. Recherche et quantification des résidus d’antibiotiques dans le muscle du Poulet de chair et dans le lait dans la région centre d’algérie, Revue Scient. et Tech. de l'OIE. 38(3), 863–877.
13. Cheong C.K., Hajeb P., Jinap S., Ismail-Fitry M.R., 2010. Sulfonamides determination in chicken meat products from Malaysia. International Food Research Journal. 17, 885–892.
14. Rikilt., 2022. Institute of food safety. poultry, meat, fish and egg scan, https://www.wur.nl/en/Research-Results/Research-Institutes/food-safety-research/Scientific-research/Analysing-food-and-feed/Animal -drug-research/Antibiotics-SCAN-test.htm, visited September 29, 2022.
15. Wageningen Food Safety Research (WFSR)., 2023. Antibiotics scan test, WUR. Available at: https://www.wur.nl/en/research-results/research-institutes/food-safety-research/scientific-research/ analysing-food-and-feed/animal-drug-research/antibiotics-scan-test.htm visited April 16, 2023.
16. Şenyuva H., Özden T., Sarica D.Y., 2000. High-performance liquid chromatographic determination of oxytetracycline residue in cured meat products. Turkish Journal of Chemistry. 24, 395-400.
17. Barton M.D., 2005. Antibiotic use in animal feed and its impact on human health. Nutritive Research Review. 13, 279–299.
18. Verdon E., Couëdor P., Sanders, P., 2004. Validation of a multi-quinolone, multi-matrix, multi-species method for the determination of quinolone residues by HPLC with fluorescence detection. AFSSA; French agency for food safety Euroresidue V, Noodwijkerhout, The Netherlands. 10- 12.
19. Boamah V.E., Agyare C., Odoi H., Dalsgaard A., 2016. Antibiotic practices and factors influencing the use of antibiotics in selected poultry farms in Ghana. Journal of Antimicrobial Agents. 2, 120-128.
20. Adla J., Nada E.D., 2019. Evaluation of antibiotics residues in chicken meat samples in Lebanon. Antibiotics (Basel). 8(2), 69-75.
21. Ramatla T., Ngoma L., Adetunji M., Mwanza M., 2017. Evaluation of antibiotic residues in raw meat using different analytical methods. Antibiotics. 6, 34-38.
22. Pena A., Silva L.J.G., Pereira A., Meisel L., Lino C.M., 2010. Determination of fluoroquinolone residues in poultry muscle in Portugal. Analytical and Bioanalytical Chemistry. 397, 2615–2621.
23. Sattar S., Hassan M.M., Islam S.K.M.A., Alam M., Al Faruk M.S., Chowdhury S., Saifuddin A.K.M., 2014. Antibiotic residues in broiler and layer meat in Chittagong district of Bangladesh. Veterin World. 7, 738–743.
24. World Health Organization, 2001. Antibiotic Resistance: Synthesis of Recommendations by Expert Policy Groups. Alliance for the Prudent Use of Antibiotics; WHO: Geneva, Switzerland, 2001.
25. Haller M.Y., Müller S.R., McArdell C.S., Alder A.C., Suter M.J.F., 2002. Quantification of veterinary antibiotics (sulfonamides and trimethoprim) in animal manure by liquid chromatography-mass spectrometry. Journal of Chromatography A. 952, 111–120.
26. Kantiani L., Farré M., Grases Freixiedas, J.M., Barceló D., 2010. Development and validation of a pressurised liquid extraction liquid chromatography-electrospray-tandem mass spectrometry method for β-lactams and sulfonamides in animal feed. Journal of Chromatography A. 1217, 4247–4254.
27. Kaulol A.S., 2018. Veterinary drug use in poultry farms and determination of antimicrobial drug residues in commercial eggs and slaughtered chicken in Kaduna state, Nigeria. International Journal of Advanced Research. 6(7), 25-29.