Quantification of Antibiotic Residues in Local and Imported Broiler Chicken Meat in Al-Batina Governorate using Liquid Chromatography-Mass Spectrometry

محورهای موضوعی :

Hiba Hamood Saif AL Kiyumi ¹ , Lama Ali Salim AL Manai ² , Dr Ahmed Abusham ³ , Salem Said Jaroof Al Touby ⁴ , Waleed Khalid Hilal Al Rajhi ⁵ , Yahya Bin Abdullah Alrashdi ⁶ , Md. Amzad Hossain ⁷

1 - School of Pharmacy, College of Health Sciences, University of Nizwa, P. O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman
2 - School of Pharmacy, College of Health Sciences, University of Nizwa, P. O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman
3 - School of Pharmacy, College of Health Sciences, University of Nizwa, P. O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman
4 - School of Nursing, College of Health Sciences, University of Nizwa, P. O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman
5 - School of Nursing, College of Health Sciences, University of Nizwa, P. O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman
6 - School of Nursing, College of Health Sciences, University of Nizwa, P. O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman
7 - School of Pharmacy, College of Health Sciences, University of Nizwa, P. O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman

تاریخ دریافت : 1402/09/14 تاریخ پذیرش : 1403/06/30 تاریخ انتشار : 1403/07/03

کلید واژه: Omani broiler chickens, Imported broiler chickens, Extraction, Antibiotics, MS/Q-TOF,

چکیده مقاله :

Antibiotics are widely used in poultry to increase meat production and prevent infections. The aim of this study is to quantitative and qualitative determination of antibiotic residue in the chicken samples. The antibiotic residues were extracted from the local and imported broiler chicken samples by applying modified established methods and quantified them by liquid chromatography-mass spectrometry (MS/Q-TOF). Both antibiotics were extracted from the meat and kidney collected from chicken using well-established methods. The extracted samples were analyzed by using the sensitive MS/Q-TOF. The experimental results showed that collected local and imported chicken samples contain five analyzed antibiotics and are within the permissible limit except for gentamicin. In the imported meat sample, the highest amount of antibiotics was, sulfanilamide, and the lowest was levofloxacin. However, in the local breast meat samples, among the analyzed antibiotics, the amount of gentamicin in both local chicken collected from Al Safwa and Waeel is too high, 0.202 m kg-1 and 369.87, respectively compared to the maximum residue level (MRL). Other antibiotics in the breast meat samples are within the MRL values. In kidney samples in the local broiler chicken, the amount of all analyzed antibiotics was within the MRL values. The highest concentration was sulfanilamide, followed by gentamicin > oxytetracycline > chloramphenicol > levofloxacin. In conclusion, all the imported and local broiler meat and kidney contains the five analyzed antibiotics within the MRL values, except gentamicin was available in high amounts in the local breast meat. In conclusion, the excessive amount of gentamicin in the chicken samples through diet will create significant health troubles.

چکیده انگلیسی:

Antibiotics are widely used in poultry to increase meat production and prevent infections. The aim of this study is to quantitative and qualitative determination of antibiotic residue in the chicken samples. The antibiotic residues were extracted from the local and imported broiler chicken samples by applying modified established methods and quantified them by liquid chromatography-mass spectrometry (MS/Q-TOF). Both antibiotics were extracted from the meat and kidney collected from chicken using well-established methods. The extracted samples were analyzed by using the sensitive MS/Q-TOF. The experimental results showed that collected local and imported chicken samples contain five analyzed antibiotics and are within the permissible limit except for gentamicin. In the imported meat sample, the highest amount of antibiotics was, sulfanilamide, and the lowest was levofloxacin. However, in the local breast meat samples, among the analyzed antibiotics, the amount of gentamicin in both local chicken collected from Al Safwa and Waeel is too high, 0.202 m kg-1 and 369.87, respectively compared to the maximum residue level (MRL). Other antibiotics in the breast meat samples are within the MRL values. In kidney samples in the local broiler chicken, the amount of all analyzed antibiotics was within the MRL values. The highest concentration was sulfanilamide, followed by gentamicin > oxytetracycline > chloramphenicol > levofloxacin. In conclusion, all the imported and local broiler meat and kidney contains the five analyzed antibiotics within the MRL values, except gentamicin was available in high amounts in the local breast meat. In conclusion, the excessive amount of gentamicin in the chicken samples through diet will create significant health troubles.

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