Studying the therapeutic effect of Oliveria decumbens vent. plant essential oil in the treatment of bladder inflammation caused by Escherichia coli in male Wistar rats
Subject Areas : Journal of Comparative PathobiologyHossein Antikchi 1 , S Mashhady Rafie 2 , Negar Panahi 3 , K Amini 4
1 - Department of Clinical Sciences, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Clinical Sciences, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - 3. Department of Microbiology, Faculty of Basic Science, Saveh Branch, Islamic Azad University, Markazi, Iran
Keywords: Oliveria decumbens, Escherichia coli, Essential oil, Antibiotic resistance,
Abstract :
Antibiotic resistance, especially in Escherichia coli, is one of the most worrying issues worldwide. In addition, the side effects of chemical drugs have led scientists to use natural alternatives with better therapeutic effects and fewer side effects than common treatments. Therefore, this research has studied the therapeutic effect of Oliveria decumbens plant essential oil in the treatment of inflammation in the bladder caused by Escherichia coli infection in male Wistar rats. The antibacterial properties of the plant extract were investigated using the standard microdilution method against Escherichia coli. Then, to cause cystitis, Escherichia coli bacteria with a concentration of 1.5 x 108 CFU/mL was injected into the bladder of mice, and then different concentrations of the extract and gentamicin were administered to them. Bladder histopathological parameters were evaluated at the end of the study. MIC and MBC of the extract against Escherichia coli were 0.32 μl/ml and 512 μl/ml, respectively. In the infected group without treatment, the infiltration of inflammatory cells and the thickness of the bladder tissue increased significantly, but in the extract-treated groups, especially at higher doses, these parameters decreased significantly (p<0.01). The effect of the extract in reducing the number of bacteria was comparable to gentamicin. In addition, after administration of the extract, inflammation, fibrosis and thickness of the epithelium also decreased in a dose-dependent manner (p<0.01). The results of this research showed that the treatment with the extract of this plant, which was prescribed as an oral supplement or subcutaneous injection, led to a significant decrease in the number of urinary bacteria and improved pathological changes in the studied mice.
1. Waldorff MS, Bjerrum L, Holm A, Siersma V, Bang C, Llor C, et al. Influence of antimicrobial resistance on the course of symptoms in female patients treated for uncomplicated cystitis caused by Escherichia coli. Antibiotics. 2022;11(2):188.
2. Waldorff MS, Bjerrum L, Holm A, Siersma V, Bang C, Llor C, et al. Influence of Antimicrobial Resistance on the Course of Symptoms in Female Patients Treated for Uncomplicated Cystitis Caused by Escherichia coli. Antibiotics (Basel). 2022;11(2).
3. Tizura AT, Material Soncini JG, Koga VL, Takayama Kobayashi RK, Tano ZN, Vespero EC. Escherichia coli Isolated from Vegans, Vegetarians and Omnivores: Antibiotic Resistance, Virulence Factors, Pathogenicity Islands and Phylogenetic Classification. Microbiology Research. 2022;13(4):825-35.
4. Altun M YB. Determination of chemical compositions and antibacterial effects of selected essential oils against human pathogenic strains. Anais da Academia Brasileira de Ciências. 2023;318:137997.
5. Falleh H, Jemaa MB, Neves MA, Isoda H, Nakajima M, Ksouri R. Formulation, physicochemical characterization, and anti-E. coli activity of food-grade nanoemulsions incorporating clove, cinnamon, and lavender essential oils. Food Chemistry. 2021;359:129963.
6. Ahmed MM, Khadum HE, Jassam HMS. Medicinal Herbs as Novel Therapies against Antibiotic-Resistant Bacteria. Research Journal of Pharmacy and Technology. 2023;16(1):62-6.
7. Malihi N, Danafar F, Moosavi-nasab M. The effect of Oliveria decumbens Vent. essential oils and lysozyme on physicochemical and functional properties
of fish gelatin film. Journal of Food Measurement and Characterization. 2022;16(3):2356-64.
8. Moghadam MJ, Maktabi S, Zarei M, Koohi PM. Inhibitory Activity of Oliveria decumbens Essential Oil on Staphylococcus aureus Biofilm and Planktonic Cells. Avicenna Journal of Clinical Microbiology and Infection. 2022;9(3):109-14.
9. Behbahani BA, Yazdi FT, Vasiee A, Mortazavi SA. Oliveria decumbens essential oil: Chemical compositions and antimicrobial activity against the growth of some clinical and standard strains causing infection. Microbial pathogenesis. 2018;114:449-52.
10. Tuken M, Temiz MZ, Yuruk E, Kaptanagasi AO, Basak K, Narter F, et al. The role of an herbal agent in treatment for Escherichia coli induced bacterial cyctitis in rats. 2017;89(2):134-8.
11. Zwaans BM, Krueger S, Bartolone SN, Chancellor MB, Marples B, Lamb LEJAiro. Modeling of chronic radiation-induced cystitis in mice. 2016;1(4):333-43.
12. Zhou Y, Zhou Z, Zheng L, Gong Z, Li Y, Jin Y, et al. Urinary Tract Infections Caused by Uropathogenic Escherichia coli: Mechanisms of Infection and Treatment Options. International Journal of Molecular Sciences. 2023;24(13):10537.
13. Ceprnja M, Oros D, Melvan E, Svetlicic E, Skrlin J, Barisic K, et al. Modeling of urinary microbiota associated with cystitis. Frontiers in cellular and infection microbiology. 2021;11:643638.
14. Hayami H, Takahashi S, Ishikawa K, Yasuda M, Yamamoto S, Wada K, et al. Second nationwide surveillance of bacterial pathogens in patients with acute uncomplicated cystitis conducted by Japanese Surveillance Committee from 2015 to 2016: antimicrobial susceptibility of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus saprophyticus. Journal of Infection and Chemotherapy. 2019;25(6):413-22.
15. Del Popolo G, Nelli F. Recurrent bacterial symptomatic cystitis: A pilot study on a new natural option for treatment. Archivio Italiano di Urologia e Andrologia. 2018;90(2):101-3.
16. Stothers LJCJoU. A randomized trial to evaluate effectiveness and cost effectiveness of naturopathic cranberry products as prophylaxis against urinary tract infection in women. 2020;9:1558-62.
17. Haverkorn MJ, Mandigers JJJ. Reduction of bacteriuria and pyuria using cranberry juice. 2021;272(8):590-.
18. Sharma A, Chandraker S, Patel V, Ramteke PJIjops. Antibacterial activity of medicinal plants against pathogens causing complicated urinary tract infections. 2019;71(2):136.
19. Mahboubi M, Feyzabadi M, Haghi G, Hosseini H. Antimicrobial activity of chemichal composition of essential oil from olivera decumbens vent. 2018.
20. Amin G, Sourmaghi MS, Zahedi M, Khanavi M, Samadi NJF. Essential oil composition and antimicrobial activity of Oliveria decumbens. 2015;76(7-8):704-7.
21. Bagamboula C, Uyttendaele M, Debevere JJFm. Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. 2019;21(1):33-42.
22. Botelho M, Nogueira N, Bastos G, Fonseca S, Lemos T, Matos F, et al. Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens. 2017;40(3):349-56.
23. Hirota R, Roger NN, Nakamura H, Song HS, Sawamura M, Suganuma N. Anti‐inflammatory effects of limonene from yuzu (Citrus junos Tanaka) essential oil on eosinophils. Journal of Food Science. 2020;75(3):H87-H92.
24. Fachini-Queiroz FC, Kummer R, Estevao-Silva CF, Carvalho MDdB, Cunha JM, Grespan R, et al. Effects of thymol and carvacrol, constituents of Thymus vulgaris L. essential oil, on the inflammatory response. 2012;2012.
25. Khoshbakht T, Karami A, Tahmasebi A, Maggi F. The variability of thymol and carvacrol contents reveals the level of antibacterial activity of the Essential Oils from different accessions of Oliveria decumbens. Antibiotics. 2020;9(7):409.
26. Zapién-Chavarría KA, Plascencia-Terrazas A, Venegas-Ortega MG, Varillas-Torres M, Rivera-Chavira BE, Adame-Gallegos JR, et al. Susceptibility of multidrug-resistant and biofilm-forming uropathogens to Mexican oregano essential oil. Antibiotics. 2019;8(4):186.
27. Kwiatkowski P, Sienkiewicz M, Pruss A, Łopusiewicz Ł, Arszyńska N, Wojciechowska-Koszko I, et al. Antibacterial and anti-biofilm activities of essential oil compounds against New Delhi Metallo-β-lactamase-1-producing uropathogenic Klebsiella pneumoniae strains. Antibiotics. 2022;11(2):147.
28. El-jalel LF, Idris HA, Eltagori A, Bumadian MM, Bozakouk IH, Gonaid MH, et al. Evaluation of the Antimicrobial Effect of Thymus capitatus Essential Oil (EO) Extract on Bacteria Isolated from Urinary Tract Infection. AJBSR. 2021;3:39-46.
