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Open Access Article
1 - Serum levels of malondialdehyde, glutathione peroxidase and superoxide dismutase in patients with non-Hodgkin's lymphoma in Zahedan
Hosnie Hoseini Parichehreh Yaghmaei Gholamreza Bahari Saeed Aminzadeh -
Open Access Article
2 - Impact of Super Absorbent Polymer on Physiological Traits and Activity of Antioxidant Enzymes in Wheat (Triticum aestivum L. cv. Mihan) Affected Drought Stress Conditions
Ahmad Afkari -
Open Access Article
3 - Effect of selenium supplementation on antioxidant indices and metabolism-related hormones in rats exposed to heat stress
Hamid Ashrafi Ali Asghar Sadeghi Mohammad ChamaniAim: High temperature causes oxidative stress in the body of animals. Selenium is a trace mineral that affects the health and performance of the body in stressful conditions. There are limited studies on the effects of different doses of selenium in long-term heat stres MoreAim: High temperature causes oxidative stress in the body of animals. Selenium is a trace mineral that affects the health and performance of the body in stressful conditions. There are limited studies on the effects of different doses of selenium in long-term heat stress conditions on physiological parameters and health indicators. This study was conducted to determine the effects of different doses of selenium from selenium-methionine supplement on the antioxidant status (glutathione concentration, activity of superoxide dismutase and glutathione peroxidase), liver and kidney health index (the activity of transferase enzymes and creatinine concentration) and concentration of metabolism-related hormones (insulin, cortisol, triiodothyronine and tetra-iodothyronine).Materials and methods: In a completely randomized design, 25 female rats were randomly divided into five groups and five replicates. Five rats were placed at standard temperature during the experiment period and the rest of the rats were placed under heat stress (38 ± 2 °C for 6 hours/day). Rats in the negative control group (without heat stress) and positive control (heat stress) were fed with standard pellets without additive and the other three groups were fed with standard pellets plus 0.15, 0.30 and 0.45 mg selenium/kg as a selenium-methionine supplement for 30 days.Results: Rats receiving selenium had more hemoglobin and alanine transaminase enzyme activity (P=0.001) compared to the positive control group. Cortisol concentration in the positive control group was higher than the negative control group (P=0.001). Adding selenium to the diet of rats under heat stress decreased (P=0.001) the concentration of cortisol, and increased the concentration of insulin and triiodothyronine compared to the positive control group. The serum malondialdehyde of the positive control was higher than the negative control and other experimental groups (P=0.001).Conclusion: According to the results of this study, heat stress causes oxidative stress in the body and causes changes in the hormonal concentration and the activity of the body's enzymes. In overall, it can be concluded that higher selenium supplementation (0.45 mg/kg) is optimal for improving the liver and kidney indices, hormone concentration and antioxidant response in rats under heat stress conditions. Manuscript profile -
Open Access Article
4 - The effect of zinc-methionine supplementation on antioxidant status and expression of interleukin-4 and interleukin-6 genes in female rats under heat stress
Matin Jamei Ali Asghar Sadeghi Mohammad ChamaniIntroduction:During heat stress, the animal body requires more antioxidant compounds and activity of antioxidant enzymes. Zinc plays a role in the structure and activity of antioxidant enzymes. One of the ways to supply the zinc requirement, is to use organic zinc suppl MoreIntroduction:During heat stress, the animal body requires more antioxidant compounds and activity of antioxidant enzymes. Zinc plays a role in the structure and activity of antioxidant enzymes. One of the ways to supply the zinc requirement, is to use organic zinc supplements, which zinc combined with methionine, and have more intestinal absorption Aim: This study was done to evaluate the effects of different doses of zinc-methionine supplementation on the blood antioxidant status and the expression of interleukin 4 and 6 genes in rats exposed to heat stress.Materials and methods: In a completely random design, 20 female rats were divided into four treatment groups with five replicates. Rats were kept at a temperature of 31±2 °C for 20 hours and at a temperature of 38±2 °C for 4 hours per day (to create heat stress). The rats in the control group were fed standard pellets without additives, and the three experimental groups were fed standard pellets plus 15, 30 and 45 mg zinc-methionine supplement per kilogram of dry matter for 30 days. At the end of the experiment, a blood sample was collected from the inferior vena cava. Total antioxidant capacity, malondialdehyde concentration, antioxidant enzymes, liver enzymes in the serum and the expression of interleukin-4 and interleukin-6 genes were measured.Results: Serum zinc concentration increased linearly with increasing zinc dose in the diet (P<0.05). The serum activity of two enzymes, alanine aminotransferase and aspartate aminotransferase decreased linearly with increasing zinc-methionine dose in the diet (P<0.01). The highest activity of glutathione reductase and glutathione peroxidase was observed in the group receiving 30 mg/kg, and the lowest activity of these enzymes was observed in the control group. In general, the relative expression of the interleukin-4 gene was increased, and the relative expression of the interleukin-6 gene was decreased (P<0.05). The highest expression of interleukin-4 gene belonged to the group receiving 30 mg, and the highest relative expression of interleukin-6 gene belonged to the control group and the group receiving 15 mg/kg.Conclusion: The results of this study showed that the dose of up to 30 mg of zinc-methionine per kg of diet used in this study increases the activity of superoxide dismutase enzyme and glutathione peroxidase enzyme activity, decreases the activity of liver enzymes in the serum, increases the expression of the anti-inflammatory gene (Interleukin-4) and decreases the expression of inflammatory gene (Interleukin-6), which indicates the reduction of oxidative stress and the reduction of the effects of heat stress in the body of the rat. Manuscript profile -
Open Access Article
5 - Wheat Response (Triticum aestivum L.) to Selenium under Normal Irrigation and Water Deficit Conditions
Mohammad Reza DadniaTo evaluate the effect of selenium (from sodium selenate) on increasing efficiency at water deficit condition a research was conducted in Karaj 1 wheat cultivar in 2014-2015 cropping year with split plot based on completely randomize block design with four replications MoreTo evaluate the effect of selenium (from sodium selenate) on increasing efficiency at water deficit condition a research was conducted in Karaj 1 wheat cultivar in 2014-2015 cropping year with split plot based on completely randomize block design with four replications in Karaj. Irrigation treatments with two levels, normal (I1) and interruption of irrigation at mid flowering (110 days after planting) (I2), which were assigned to main plots and selenium foliar application with six concentrations: 0 (S0), 5 (3.12 micro g.) (S1), 10 (6.24 micro g.) (S2), 15 (9.36 micro g.) (S3), 20 (12.5 micro g.) (S4) and 25 (15.62 micro g,) of sodium selenate per plant (S5) g.ha-1 to sub plots. The results showed that water deficit increased the rate of superoxide dismutase, catalase, glutathione peroxidase and malondialdehyde marker. Foliar application of selenium, increased contents of antioxidant (anti oxidation) enzymes under stress condition and was maximum in 20 g. selenium per ha-1. Content of malondialdehyde was decreased and caused significant increase in grain yield. Increasing antioxidants and reduction of malondialdehyde at normal condition was lower. On the other hand, negative effect of malondialdehyde on yield components, cellular membrane stability and antioxidant enzymes at humidity stress condition was higher than normal condition. Significant increase of cytoplasmic membrane stability, grain yield, grain weight and selenium content of seed was obtained when 15 and 20 g. selenium ha-1 were used as compared with those of higher and lower rates of selenium usage under water deficit and normal irrigation. Suppression of free oxygen radicals, and enzyme induction defense mechanisms against oxidative damage has been effective. Therefore, use of exogenous compounds such as selenium may increase the antioxidant capacity of plant against stress. Manuscript profile -
Open Access Article
6 - Effect of Humic Acid on Activity of Antioxidant Enzymes and Yield of Castor Bean (Ricinus commonis) under Water Deficit Condition
Mohammad Reza DadniaTo evaluate the effect of humic acid on activity of antioxidant enzymes in castor bean a split plot experiment based on completely randomize block design with four replications was carried out at Islamic Azad University, Karaj Branch in 2014. Treatments were irrigation MoreTo evaluate the effect of humic acid on activity of antioxidant enzymes in castor bean a split plot experiment based on completely randomize block design with four replications was carried out at Islamic Azad University, Karaj Branch in 2014. Treatments were irrigation with three levels [irrigation at 80% of field capacity (S1) (normal), irrigation at 65% of field capacity (S2) (mid stress) and irrigation at 50% of field capacity (S3) (high stress)] assigned to main plots and humic acid with four levels (without foliar application, one time, twice and three times foliar application) to sub plots. In this study traits like seed yield, 100 seed weight and antioxidant enzymes were estimated. The results showed that the effect of humic acid was significant at 1% level on 100 seed weight and activity of antioxidant enzymes. Mean comparisons indicate that foliar application of humic acid could compensate relative effect of water deficit. It was also revealed that the rate of superoxide dismutase, glutathione peroxidase and catalase were increased with three times of humic acid foliar applications by 38.8, 34.75 and 37.88 percent under mid stress drought and 26.63, 41.15 and 43.14 percents under high stress drought as compared to control, respectively. Overall, it can be said that use of humic acid have different physiological effects. Humic acid not only increases seed yield of castor bean it also have a positive role in reducing water deficit effects and increasing activity of antioxidant enzymes. Manuscript profile -
Open Access Article
7 - Comparison of Serum Levels of Vitamin C, Vitamin E, Selenium and the Activity of Glutathione Peroxidase Enzyme in Horses Affected with Strangles and Healthy Horses
Mohammadreza Valilou Sina Moghaddam Ali HassanpourStrangles is a prevalent infectious and contagious disease among Equidae that is caused by Streptococcus equi subspecies equi. The present study was performed on patient and healthy horses to evaluate serum levels of vitamin C (vitamin C), vitamin E (vitamin E), seleniu MoreStrangles is a prevalent infectious and contagious disease among Equidae that is caused by Streptococcus equi subspecies equi. The present study was performed on patient and healthy horses to evaluate serum levels of vitamin C (vitamin C), vitamin E (vitamin E), selenium (Sel) and glutathione peroxidase (GPx). Clinical signs and bacterial culture confirmed sixty patient horses. To separate the serum, blood samples were taken from the jugular vein of the studied horses. Blood samples were also taken from 52 healthy horses with similar conditions (age, diet, etc.). Serum levels of Vit E, Vit C, Se and serum’s activity of GPx were measured in samples taken from healthy and patient horses. The mean serum concentration of vitamin E, selenium and serum glutathione peroxidase activity in patient horses were significantly reduced. A significant increase in the patient's hemoglobin concentration was observed. The study of the relationship between serum selenium and antioxidants (serum glutathione peroxidase activity, vitamin E and hemoglobin) showed that increasing serum selenium also increased the antioxidants studied. Serum antioxidant markers were significant in patient horses in all three cases. The final outcome is that in horses which infected to strangles antioxidants like selenium, glutathione peroxidase and vitamin E reduced and these antioxidant intakes for dealing with them this issue should be considered in treating these patients. Manuscript profile -
Open Access Article
8 - The effect of eight weeks of propolis consumption during resistance training on the Glutathione peroxidase activity of kidney tissue of male rats consuming testosterone enanthate
masoumeh mehrabi yaser kazemzadeh Seyed Ali hoseini Saeid SedaghatiIntroduction: Anabolic androgenic steroids are often used by athletes to improve sports performance. Kidney damage has been reported as a side effect of abuse of these substances. Therefore, the aim of the present study is to investigate the effect of propolis consumpti MoreIntroduction: Anabolic androgenic steroids are often used by athletes to improve sports performance. Kidney damage has been reported as a side effect of abuse of these substances. Therefore, the aim of the present study is to investigate the effect of propolis consumption during eight weeks of resistance training on Glutathione peroxidase activity in kidney tissue in male rats consuming testosterone enanthate.Methodology: In this experimental study, 32 male mice with an age of 8 weeks and an average weight of 250 ± 50 grams were prepared. after two weeks of familiarization and weighing twice a week, the mice were randomly divided into 4 groups: Control(C), sham(RT), resistance training +testosterone(RT+T) and resistance training+testosterone+propolis(RT+T+P) group were divided. The resistance training program was carried out 5 days a week with two rest days a week. The weights were 60% of body weight in the first week and 20% of body weight was added every week. Rats receiving steroids received 20 mg of testosterone enanthate intramuscularly. The receiving group received 400 mg of propolis cleanser by gavage. After dissection, kidney tissue was sampled to measure GPX concentration. Data analysis was done using one-way analysis of variance with SPSS24.Findings: The results of one-way analysis of variance showed that there is a significant difference in the research groups (P=0.0001). According to Bonferroni's post hoc test, GPX levels in the exercise+testosterone and exercise+testosterone+propolis groups have significantly decreased compared to the control and sham groups.Discussion and conclusion: It seems that testosterone consumption can be effective in increasing oxidative stress factors in the kidney tissue of resistance-trained rats. The use of propolis to treat the side effects of steroids has not had the desired effect in the short term. Manuscript profile -
Open Access Article
9 - Effect of foliar application of ascorbic acid on antioxidant enzyme activities of mung bean (Vigna radiate L.) cultivars under water-deficit Stress
Mohammad Jahanbakhshi Mehdi Sadeghi Mahmood Tohidi Farbod Fotouhi Seyed Ali FazelzadehIn order to investigate the effect of foliar application of ascorbic acid on the activity of antioxidant enzymes in mung bean (Vigna radiate L.) under water-deficit stress, a field trial was performed in a split-plot design arranged in completely randomized block design MoreIn order to investigate the effect of foliar application of ascorbic acid on the activity of antioxidant enzymes in mung bean (Vigna radiate L.) under water-deficit stress, a field trial was performed in a split-plot design arranged in completely randomized block design with three replications in the Education and Research Center of Natural Resources Safiabad, Dezful during the two cultivation years (2017 and 2018). Four levels of water-deficit stress treatments were considered in the main plots (25, 50, 75, and 100% of plant water requirement) and combined treatment of foliar application of ascorbic acid in three levels (control, 10, and 20 mM) and genotype including Omidbakhsh VC6173 and Partow cultivars as subplots. The results of mean comparison showed that the highest levels of superoxide dismutase and ascorbate peroxidase were observed in water-deficit stress of 25% water requirement and no foliar application of ascorbic acid. The highest activity of catalase enzyme was observed under water-deficit stress of 50% water requirement and no foliar application of ascorbic acid. Also, the highest activity of guaiacol peroxidase enzyme was observed in Omidbakhsh VC6173 cultivar under water-deficit stress of 25% water requirement and no foliar application of ascorbic acid. The highest glutathione peroxidase activity was observed in VC6173 cultivar under water-deficit stress of 75% water requirement and 10 mM foliar application of ascorbic acid. Results showed that dehydration stress significantly increased the activity of antioxidant enzymes while foliar application of ascorbic acid due to its antioxidant properties reduced the effects of stress and thus reducing the activity of antioxidant enzymes Manuscript profile -
Open Access Article
10 - اثرات آنتی بیوتیکی و آنتی اکسیدانی عصاره متانولی برگ گیاه Momordica charantia بر فشار خون ناشی از آلوکسان در موش صحرایی
ساندی افوگ الوفکه فلایی بلسین اگونپلو آدمولا اویابمی تمیدایو اوموبوال مومو یکوبو اولووافمی اوگونتیبجو آدلو آدداپوBackground & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momo MoreBackground & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momordica charantia (MEMC) on alloxan-induced diabetic rats. Experimental: The antidiabetic influence of methanol leaf extract of Momordica charantia (MEMC) was investigated in alloxan-induced diabetes in rats. 50 rats allocated into five groups (A-E) (n =10) were utilized in this study: group A was normal control, groups B to E were induced with alloxan with diabetes established, while group B was not treated, group C received glibenclamide treatment while groups D and E were administered extracts at 200 and 400 mg/kg doses, respectively, in a study that continued for 28 days. Changes in blood glucose levels, body weight, haematology, serum chemistry, antioxidant system and histopathology changes were evaluated. Results: MEMC elicited significant drop in blood glucose level from diabetic to near normal level, restoring the body weight, haematological and serum biochemical parameters to the basal non diabetic level; likewise the MEMC-treated group elicited a decreased level of oxidant markers such as malondialdehyde (MDA) and hydrogen peroxide (H2O2) but increased concentration of protein thiols (PT), non-protein thiols (NPT), glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. The pancreas section also revealed repair of distorted pancreatic architecture in MEMC-treated group compared to diabetic group. Recommended applications/industries: The plant exhibited anti-hyperglycaemic, anti-diabetic and anti-oxidant abilities hence could be explored for the treatment of diabetes mellitus. Manuscript profile -
Open Access Article
11 - تأثیر منابع سلنیوم غیرآلی و آلی روی عملکرد و کیفیت گوشت جوجههای گوشتی
کا. راجاشری تی. موتوکومار ان. کارتیکیاناین آزمایش برای ارزیابی و مقایسه اثر منابع سلنیوم غیرآلی و آلی در جوجههای گوشتی (راس 308) انجام شد. جوجههای گوشتی نر یکسان در پنج گروه با شش تکرار از جیره بدون مکمل سلنیوم (کنترل منفی)، یا جیرههای مکمل شده با سلنیوم آلی و غیرآلی در سطوح 25/0ppm سلنیوم غیرآلی، Moreاین آزمایش برای ارزیابی و مقایسه اثر منابع سلنیوم غیرآلی و آلی در جوجههای گوشتی (راس 308) انجام شد. جوجههای گوشتی نر یکسان در پنج گروه با شش تکرار از جیره بدون مکمل سلنیوم (کنترل منفی)، یا جیرههای مکمل شده با سلنیوم آلی و غیرآلی در سطوح 25/0ppm سلنیوم غیرآلی، 5/0ppm سلنیوم غیرآلی، 25/0 ppm سلنیوم آلی و 5/0 ppm سلنیوم آلی تغذیه شدند. سدیم سلنات و مخمر غنی از سلنیوم به عنوان منابع غیرآلی و آلی سلنیوم استفاده شدند. طیور به مدت 35 روز پرورش داده شدند و برای عملکرد رشد، کیفیت لاشه و اندامهای لنفوئیدی مورد ارزیابی قرار گرفتند. نمونههای گوشت و کبد برای گلوتاتیون پراکسیداز، مواد واکنش دهنده با تیوباربیتیوریک اسید، pH گوشت و ظرفیت نگهداری آب مورد آنالیز قرار گرفتند. نتایج نشان داد که مکمل سلنیوم صرف نظر از منبع و سطح استفاده باعث بهبود عملکرد جوجههای گوشتی توسط افزایش مصرف خوراک و وزن بدن گردیدند. تفاوت معنیداری در ضریب تبدیل خوراک، درصد زندهمانی و صفات مرتبط با لاشه در بین گروههای تیماری یافت نشد (05/0P<). سلنیوم آلی در سطح 5/0 ppm به عنوان یک منبع عالی از سلنیوم تعیین شد به طوری که به واسطه افزایش ابقاء سلنیوم، فعالیت بیشتر گلوتاتیون پراکسیداز و کاهش نرخ پراکسیداسیون لیپید کیفیت گوشت را بهبود داد. به علاوه، مکمل سلنیوم از منشأ آلی در سطح 5/0ppm باعث بهبود ظرفیت نگهداری آب گوشت شد. Manuscript profile -
Open Access Article
12 - اثر تیمول و کارواکرول بر عملکرد تولید، فعالیت آنزیمهای آنتیاکسیدانی و متابولیتهای خونی جوجههای گوشتی تحت تنش گرمایی
ح. سعادت شاد م. مظهری ا.ع. اسماعیلیپور ح. خسروینیامطالعه حاضر به منظور بررسی اثر تیمول و کارواکرول بر عملکرد تولید، فعالیت آنزیمهای آنتیاکسیدانی و متابولیتهای خونی جوجههای گوشتی تحت تنش گرمایی طراحی شد. جوجهها تا 25 روزگی با جیره استاندارد تجاری تغذیه شده و سپس به یک آزمایش فاکتوریل (2×3) در قالب طرح کاملاً Moreمطالعه حاضر به منظور بررسی اثر تیمول و کارواکرول بر عملکرد تولید، فعالیت آنزیمهای آنتیاکسیدانی و متابولیتهای خونی جوجههای گوشتی تحت تنش گرمایی طراحی شد. جوجهها تا 25 روزگی با جیره استاندارد تجاری تغذیه شده و سپس به یک آزمایش فاکتوریل (2×3) در قالب طرح کاملاً تصادفی با سه سطح کارواکرول (0، 300 و500 میلیگرم در هرکیلوگرم خوراک) و دو سطح تیمول (0 و250 میلیگرم درهرکیلوگرم خوراک) در چهار تکرار نه پرندهای اختصاص یافتند. جهت ایجاد تنش گرمایی، جوجهها از 26 روزگی تحت دمای 35 درجه سانتی­گراد به مدت 8 ساعت در روز قرار گرفتند. نتایج آزمایش نشان داد که مصرف کارواکرول اثر معنیداری بر پارامترهای عملکردی نداشت، اما مصرف 250 میلی­گرم تیمول منجر به افزایش معنیدار وزن روزانه و کاهش معنیدار ضریب تبدیل غذایی به میزان 4 و 6 درصد شد (05/0>P). ­گلبول­های سفید، قرمز، هموگلوبین، هماتوکریت و لنفوسیت تحت تأثیر تیمارها قرار نگرفتند، اما هتروفیل و نسبت هتروفیل به لنفوسیت با مصرف تیمول و کارواکرول به طور معنیداری کاهش یافت (05/0>P). مصرف تیمول و کارواکرول، کلسترول سرم خون را کاهش داد (05/0>P)، اما اثری بر تریگلیسرید و لیپوپروتئینهای با چگالی کم و زیاد و گلوکز خون نداشت. هر دو مکمل تیمول و کارواکرول به طور معنیداری فعالیت آنزیم گلوتاتیون پراکسیداز را در مقایسه با گروه شاهد افزایش دادند (05/0>P). درصد لاشه و سینه با مکمل تیمول افزایش یافت (05/0>P)، اما اثری بر وزن نسبی اندامهای داخلی مشاهده نشد. بنابراین مصرف 250 میلی­گرم تیمول میتواند از طریق بهبود عملکرد و پتانسیل آنتیاکسیدانی خون در جهت رفع اثرات منفی تحت تنش گرمایی مؤثر باشد. Manuscript profile -
Open Access Article
13 - Selenium Supplementation Efficacy in Small Ruminants: A Review
A.B. Amin R. Audu A.A. Ibrahim M. Dalha M.T. Aleem A.I. Abdullahi -
Open Access Article
14 - Review on Properties of Selenium in Animal Nutrition
ش. قادرزاده ف. میرزایی آقجه قشلاق س. نیک بین ب. نوید شاد -
Open Access Article
15 - Effects of Horsetail (Equisetum arvense), Vitamin C and Organic Zinc Supplements on Growth Performance, Carcass Yield, Serum Biochemical Values and Antioxidant Status of Broiler Chickens
S. Azimi-Youvalari M. Daneshyar S.M. Abtahi-Froushani S. Payvastegan -
Open Access Article
16 - Evaluation of proline content and enzymatic defense mechanism in response to drought stress in rice
Borzo Kazerani Saied Navabpour Hossein Sabouri Seyedeh Sanaz Ramezanpour Khalil Zaynali Nezhad Ali Eskandari -
Open Access Article
17 - Effect of hydro-ethanolic extract of Cucumis melo seeds on hepatic expression of genes encoding NADPH-oxidase and glutathione peroxidase in ethylene glycol-induced toxicity in male rats
Mehrnoosh Sadat Hoseinii Siamak Yousefi Siahkalroodi Maryam EidiIn the present study, the effect of hydroanthanolic extract of melon seed on the expression level of NOX4 and GPx1 genes in ethylene glycol poisoned mice was evaluated. In the present study, melon seed extract was first prepared by soaking in 80% ethanol. Mice were divi MoreIn the present study, the effect of hydroanthanolic extract of melon seed on the expression level of NOX4 and GPx1 genes in ethylene glycol poisoned mice was evaluated. In the present study, melon seed extract was first prepared by soaking in 80% ethanol. Mice were divided into 5 groups in each group 6 rat: a healthy control group, a poisoned control group and three experimental poisoned groups. To the experimental poisoned group, concentrations of 150, 300 and 600 mg/ kg of hydroethanolic extract of melon seed were fed orally. The results of the present study show that daily oral administration of ethylene glycol significantly increases the expression of genes encoding NOX4 and GPx1 in toxic groups compared to healthy controls. Increased expression of NOX4 and GPx1 genes indicates the occurrence of ethylene glycol-induced inflammation and toxicity. Treatment with hydroethanolic extract of melon seed at concentrations of 150, 300 and 600 mg / kg body weight significantly reduced the expression of NOX4 and GPx1 genes in the experimental groups compared with the control group. Decreased expression of NOX4 gene in comparison with the poisoned control group shows that administration of hydroethanolic extract of melon seed has reduced inflammation caused by ethylene glycol toxicity. Due to the fact that the amount of H2O2 increases following hepatotoxicity caused by ethylene glycol, the expression of the gene encoding glutathione peroxidase increases. Following treatment with melon hydroethanolic extract, H2O2 production is reduced, which may be due to the effects of antioxidant compounds in the extract. Manuscript profile -
Open Access Article
18 - Antioxidant effect of Spirulina fed Rainbow trout (Oncorhynchus mykiss) by the Enzyme Glutathione proxidase
E. Emitazjo Z. Dehghani Motalagh M. ZeinaliSpirulina is filamentous cyanobacteria and is used in aquaculture industry. In this investigation antioxidative effects of fish trouts(Onchronchus mykiss) feeded with Spirulina was studied. Changes MoreSpirulina is filamentous cyanobacteria and is used in aquaculture industry. In this investigation antioxidative effects of fish trouts(Onchronchus mykiss) feeded with Spirulina was studied. Changes in Glutathione peroxidase enzyme (GPX) was employed for this evaluation. Main stages of this investigation was performed in fish trout culture farm in Tehran. One hundred and twenty trouts with 17±2 gram weight were feeded in 5 treatments. Feed treatments included blank1 (without Spirulina), blank2 binder (without Spirulina) and three different doses of Spirulina 2.5%,5%,7.5% with binder. Spirulina with colza oil binder was mixed with food after weighing required amount of food for every pool. Fish were then fed 3 times a day for 90 day. After sampling , the special activity of GPx in liver, spleen and muscle tissue was investigated using Wendell's method. This assay indicated that increase in Spirulinapercentage in diet to 7.5 and 5% , enhanced amount of glutathione peroxidase activity in spleen to 61.52%. Also with increase in the amount of feeding with Spirulina , amount of glutathione peroxidase specific activity declined to about 21.95% in liver tissue and to 9.4% in muscles. Results of this study indicated that feeding fish with Spirulina resulted in enhancement of antioxidant activity of GPx enzyme in spleen and reduction of its activity in muscle and liver tissues of trout. . Manuscript profile -
Open Access Article
19 - Relation of water deficit stress and biofertilizer on some of antioxidant enzymes activity and role them on grain yield variation of barley (Hordeum vulgare)
Mohammadreza DadniaTo evaluate the qualitative traits of barley in response to biofertilizers (Azotobacter, Pseudomonas and Azospirillum) with affected by different regimes of irrigation the experiment was carried out in Karaj Azad University research field in 2013 with split plot based o MoreTo evaluate the qualitative traits of barley in response to biofertilizers (Azotobacter, Pseudomonas and Azospirillum) with affected by different regimes of irrigation the experiment was carried out in Karaj Azad University research field in 2013 with split plot based on Completely Randomize Block Design with four replications. In this experiment irrigation treatments were in main plots with three levels which interrupt of irrigation at 80, 65 and 50% of field capacity (normal irrigation), (35 and 50 percent of humidity discharge) and sub plots contained seed inoculation with bacteria such as |inoculation with Azospirillum lipoferum, Azotobacter chrococcum, Pseudomonas putida, Pseudomonas×Azotobacter, Pseudomonas×Azospirillum, Azotobacter×Azospirillum | and control (un inoculation) in seven levels. The results of analysis of variance showed that biofertilizer had significant effect on antioxidant enzymes at 1% probability. The activity of antioxidant enzymes affected with biofertilizers were increased at water deficit condition which Azospirillum lipoferum caused increasing about 16.7% and 21.4% and Pseudomonas×Azospirillum caused increasing about 18.2% and 25.9% for superoxide dismutase and glutathione peroxidase in irrigation at 50% of field capacity, respectively than control. Yield showed low decrease in irrigation at 50% of field capacity than normal irrigation such as yield decreasing in irrigation at 50% of field capacity was only about 13.2% with affected by Azospirillum than normal irrigation. The data showed that biofertilizer have efficient role on barley yield at water deficit condition. Manuscript profile -
Open Access Article
20 - Studying the effect of irrigation regimes and superabsorbent levels on the antioxidant enzymes on the relative permeability of cell membranes in sorghum (Sorghum bicolor L.)
mansour fazeli rostampour مسعود فاضلی رستم پورIn order to evaluate the effect of irrigation regimes and superabsorbent polymer (SAP) on amount of antioxidant enzymes, leaf relative water content and relationships between them with relative permeability of cell membranes in sorghum, the experiment was conducted in D MoreIn order to evaluate the effect of irrigation regimes and superabsorbent polymer (SAP) on amount of antioxidant enzymes, leaf relative water content and relationships between them with relative permeability of cell membranes in sorghum, the experiment was conducted in Dashtak region of Zahedan during 2013 and 2014 seasons. The experimental design was a split plot with two factors including four irrigation regimes )40, 60, 80 and 100% crop evapotranspiration( as main plots, four amounts of SAP )0, 75, 150 and 225 kg ha-1( as subplots in a completely randomized block design with three replications. The results indicated that the effect of irrigation regimes, SAP levels and interaction effects of two factors were significant. The regression models estimated for superoxide dismutase, ascorbate peroxidase, glutathione peroxidase, catalase and RPCM showed that showed that by increasing the amount of irrigation and SAP, the traits were decreased. In addition, by decreasing in leaf relative water content, the antioxidant enzymes and relative permeability of cell membranes increased. The experiment findings indicate that at the application 75 kg ha-1 SAP and supplying 80% of sorghum water requirement, the relative permeability of cell membranes was 59.83, that was similar to 100% irrigation along with the application 150 and 225 kg ha-1 SAP. Manuscript profile -
Open Access Article
21 - Effect of Tannery Effluents on the Antioxidant Enzymes of a Fresh Water Fish Channa striatus
Sivachandran Ramachandran Navaneetha Lakshmi Krishnan Priya Ponmudi -
Open Access Article
22 - The Effect of Scrophularia striata Hydroalcoholic Extract in the Prevention of Gastric Ulcers Induced by Indomethacin in Rats
Sh. Ferdowsi Z. Hooshmandi E. ShahmoradiA peptic ulcer is a common disease. One of the goals of therapy is using drugs with fewer side effects. This study aimed to investigate the effect of Scrophularia strata (Scrophularia) extract in the prevention of gastric ulcers induced by indomethacin in rats. Overall, MoreA peptic ulcer is a common disease. One of the goals of therapy is using drugs with fewer side effects. This study aimed to investigate the effect of Scrophularia strata (Scrophularia) extract in the prevention of gastric ulcers induced by indomethacin in rats. Overall, 64 male Wistar rats were divided into 8 groups. Experimental groups including 1- control group that received normal saline 0.9% and at the last day received 48 mg indomethacin. 2- Ascorbic acid 50 mg/kg -indomethacin treatment; 3- Omeprazole 10 mg/kg-indomethacin treatment; 4- Ranitidine 50 mg/kg-indomethacin treatment; 5- S. strata 10 mg/kg –indomethacin treatment; 6- S. strata 50 mg/kg -indomethacin treatment; 7- S. strata 100 mg/kg-indomethacin treatment; 8- S. strata 150 mg/kg-indomethacin treatment. After macroscopic examination, stomach tissue was studied to examine the enzymes catalase, glutathione peroxidase, and superoxide dismutase. Catalase and glutathione peroxidase activity levels in S. strata 50, 100 and 150 mg/kg treatment was significantly increased compared with the control group (P<0.001). The superoxide dismutase activity in S. strata 100 and 150 mg/kg treatment was significantly increased compared with the control group (P<0.001). Stomach tissue damage significantly decreased in the group receiving 100 and 150 mg/kg S. strata and group receiving ranitidine and indomethacin compared to control (P<0.001). S. strata extract increased catalase, glutathione peroxidase, and superoxide dismutase and dose-dependently prevented the stomach tissue damage (by increasing the level of endogenic antioxidant). Manuscript profile -
Open Access Article
23 - Effects of Silver Nanoparticles on Oxidative Stress in Adult Female Wistar Rats
N. Nowrozi E. Samani Jahromi S. Zolghadri JahromiWe aimed to evaluate the antioxidant effect of silver nanoparticles on oxidative stress in adult female of Wistar rats. In this experimental study, 50 adult female of Wistar rats were randomly divided into 5 groups. The control group (no treatment), sham group (0.2 mg/k MoreWe aimed to evaluate the antioxidant effect of silver nanoparticles on oxidative stress in adult female of Wistar rats. In this experimental study, 50 adult female of Wistar rats were randomly divided into 5 groups. The control group (no treatment), sham group (0.2 mg/kg physiology serum), experimental group 1 (50 mg/kg isoniazid), experimental group 2 (50 mg/kg isoniazid and 0.25 mg/kg silver nanoparticles) and the experimental group 3 (50 mg/kg isoniazid and 0.5 mg/kg silver nanoparticles) were given. All injections were prescribed for 15 d and isoniazid and silver nanoparticles were injected in gavage and intraperitoneally, respectively. Then all animals were anesthetized and blooded. In the end, the data were examined by ANOVA test at a significance level of P<0.05 using SPSS software. Isoniazid increases nitric oxide, reduces glutathione peroxidase activity and total antioxidant capacity. While treating the animals with silver nanoparticles reduced the side effects of oxidant-induced by isoniazid, with decreasing nitric oxide, increased glutathione peroxidase and total antioxidant capacity by reducing nitric oxide production and increasing glutathione peroxidase activity oxidative, silver nanoparticle prevent from oxidative damages and the destruction of red blood cell (RBC) membrane. In other words, the tests showed the antioxidant role of silver nanoparticles as well. Manuscript profile -
Open Access Article
24 - Effect of Nano-selenium particles and sodium selenite on performance, carcass characteristics and antioxidant enzymes of quails under heat stress
Ebrahim Talebi Reyhane Ghazanfarpoor This study was designed to investigate the effects of Nano-Selenium and Sodium Selenite on performance, carcass characteristics, Glutathione Peroxidase and Superoxide dismutase of Japanese quail under heat stress condition. In this investigation, 300 Japanese qua More This study was designed to investigate the effects of Nano-Selenium and Sodium Selenite on performance, carcass characteristics, Glutathione Peroxidase and Superoxide dismutase of Japanese quail under heat stress condition. In this investigation, 300 Japanese quail chicks were used and the chickens were raised to 21 days in the same condition. The experiment was performed in a randomized complete block design (RCBD) with 3 treatments and 5 replicates of 20 chicks per replicate. The chickens daily were included under heat stress condition for 8 h at 34± 0.5oC. The results showed that weight gain and feed intake of chicks fed diets with Selenium Nano particles compared to the other two groups was significant (p < 0.05) as well as the FCR between different groups was no significant difference (p>0.05). The all three different treatments showed no significant effect on carcass composition (p>0.05) and increase in use of Nano-Selenium was more tender than sodium Selenite group, but not significant. The results revealed that glutathione peroxidase and superoxide dismutase activity increased significantly in second experimental group using selenium (p < 0.05). In the present study, superoxide dismutase activity was significantly increased after adding 0.2 mg sodium selenite and nano-selenium (p < 0.05). The group of hemoglobin using selenium sources was significantly increased, but not significant increase in hematocrit. Results in this investigation revealed that Nano-Selenium is a better alternative compare to sodium Selenite. Manuscript profile -
Open Access Article
25 - Protective Effect of Coenzyme Q10 and Vitamin C on Cysteamine�Induced Lipid Peroxidation
سروش Almasi بابک Rezvanjoo S.H Shirazibeheshtiha علی Namvaran AbbasAbad مهدی khosraviBenefts of antioxidant supplementation in various disorders through reducing free radicals and improving organsperformance have been reported. The aim of this study was to investigate the effects of coenzyme Q10 and vitaminC on Cysteamine-induced lipid peroxidation and MoreBenefts of antioxidant supplementation in various disorders through reducing free radicals and improving organsperformance have been reported. The aim of this study was to investigate the effects of coenzyme Q10 and vitaminC on Cysteamine-induced lipid peroxidation and oxidative stress.This experimental interventional study was conducted on male Wistar rats. Animals were divided into four groups(six rats) randomly. Groups were treated as; group 1 (Normal saline), Group 2 (Cysteamine), Group 3 (vitaminC plus Cysteamine), Group 4 (coenzyme Q10 plus Cysteamine). 24 hours after the last injection, rats wereanesthetized and sampled for investigations. Welch’s and Kruskal-Wallis tests were used for analyzing data and P< 0.05 was set the signifcance level.The results of this study indicate that injection of cysteamine signifcantly (P < 0.05) decreased glutathioneperoxidase activity compared with control group. Pretreatment with vitamin C signifcantly (P < 0.05) increasedglutathione peroxidase activity compared with cysteamine group. Pretreatment with coenzyme Q10 increasedglutathione peroxidase activity (P <0.001) and superoxide dismutase (P <0.05) signifcantly compared withcysteamine group.Based on the results of this study, coenzyme Q10 and vitamin C can be used in reducing oxidative stress inducedcysteamine. Manuscript profile
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