Effect of foliar application of ascorbic acid on antioxidant enzyme activities of mung bean (Vigna radiate L.) cultivars under water-deficit Stress
Subject Areas : Genetic
Mohammad
Jahanbakhshi
1
(Department of Agronomy, Dezful Branch, Islamic Azad University, Dezful, Iran.)
Mehdi
Sadeghi
2
(Department of Agronomy, Dezful Branch, Islamic Azad University, Dezful, Iran.)
Mahmood
Tohidi
3
(Department of Agronomy, Dezful Branch, Islamic Azad University, Dezful, Iran.)
Farbod
Fotouhi
4
(Department of Agronomy, Dezful Branch, Islamic Azad University, Dezful, Iran.)
Seyed Ali
Fazelzadeh
5
(Department of Agronomy, Dezful Branch, Islamic Azad University, Dezful, Iran.)
Keywords: Ascorbic acid, Foliar application, Water deficit stress, ascorbate peroxidase, Catalase, Antioxidant system, Glutathione peroxidase,
Abstract :
In order to investigate the effect of foliar application of ascorbic acid on the activity of antioxidant enzymes in mung bean (Vigna radiate L.) under water-deficit stress, a field trial was performed in a split-plot design arranged in completely randomized block design with three replications in the Education and Research Center of Natural Resources Safiabad, Dezful during the two cultivation years (2017 and 2018). Four levels of water-deficit stress treatments were considered in the main plots (25, 50, 75, and 100% of plant water requirement) and combined treatment of foliar application of ascorbic acid in three levels (control, 10, and 20 mM) and genotype including Omidbakhsh VC6173 and Partow cultivars as subplots. The results of mean comparison showed that the highest levels of superoxide dismutase and ascorbate peroxidase were observed in water-deficit stress of 25% water requirement and no foliar application of ascorbic acid. The highest activity of catalase enzyme was observed under water-deficit stress of 50% water requirement and no foliar application of ascorbic acid. Also, the highest activity of guaiacol peroxidase enzyme was observed in Omidbakhsh VC6173 cultivar under water-deficit stress of 25% water requirement and no foliar application of ascorbic acid. The highest glutathione peroxidase activity was observed in VC6173 cultivar under water-deficit stress of 75% water requirement and 10 mM foliar application of ascorbic acid. Results showed that dehydration stress significantly increased the activity of antioxidant enzymes while foliar application of ascorbic acid due to its antioxidant properties reduced the effects of stress and thus reducing the activity of antioxidant enzymes
Abdul Jaleel, C. Riadh, K. Gopi, R. Manivannan, P. Ines, J. Al-Juburi, H. J. Chang-Xing, Z. Hong-Bo, S. and Panneerselvam, R. (2009). Antioxidant defense responses: physiological plasticity in higher plants under abiotic constrains. Acta Physiologia Plantarum 31: 427-436.
Afkari, A. (2017). Effect of seed priming on germination characteristics and some antioxidant enzymes activity of Basil (Ocimum basilicum L.) under drought stress conditions. Journal of Developmental Biology. 9)3:(33-44.
Ahmad, P. Jaleel, C.A. Salem, M. A. Nabi, G. and Sharma, S. (2010). Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Critical Reviews in Biotechnology. 30 (3):161-175.
Ahmed, S. Nawata, E. Hosokawa, M. Domae, Y. and Sakuratani, T. (2002). Alterations in photosynthesis and some antioxidant enzymatic activity of mung bean subjected to waterlogging. Journal of Plant Science. 163: 117-123.
Akram, N. A., Shafiq, F. and Ashraf, M. (2017). Ascorbic acid-a potential oxidant scavenger and its role in plant development and abiotic stress tolerance. Frontiers in plant science. (8): 601-613.
Alderfasi, A. A., Alzarqaa, A. A., Al-Yahya, F. A., Roushdy, S. S., Dawabah, A. A. and Alhammad, B. A. (2017). Effect of combined biotic and abiotic stress on some physiological aspects and antioxidant enzymatic activity in mungbean (Vigna radiate L.). Africal Journal Agricalture Research. 12 (9): 700-705.
Allen, R. G., Pereira, L. S., Raes, D., and Smith, M. (1998). Crop evapotranspiration-Guidelines for computing crop water requirements-FAO Irrigation and drainage paper 56. Fao, Rome, 300. (9). D05109.
Amini, F. Askari, M. and Haghir, M. (2017). Changes in protein and antioxidant system of Cucumis sativus cv. Isfahani in response to drought stress. Journal of Cell & Tissue (JCT). 7(4): 375-386.
Arab, P. Bradaran Firouzabadi, M. Asghari, H. Gholami, A. and Rahimi, M. (2011). The effect of ascorbic acid and sodium nitroposide spraying on some safflower characteristics under irrigation tension. Proceedings of the 12th Iranian Congress of Plant Breeding. Islamic Azad University. Karaj Branch. Karaj. 16-14.
Aydinsakir, K., Erdal, S., Buyuktas, D., Bastug, R. and Toker, R. (2013). The influence of regular deficit irrigation applications on water use, yield, and quality components of two corn (Zea mays L.) genotypes. Agricultural water management. (128): 65-71.
Basu, S., Ramegowda, V., Kumar, A. and Pereira, A. (2016). Plant adaptation to drought stress. F1000 Research, 5.
Bangar, P., Chaudhury, A., Tiwari, B., Kumar, S., Kumari, R. and Bhat, K. V. (2019). Morphophysiological and biochemical response of mung bean [Vigna radiata (L.) Wilczek] varieties at different developmental stages under drought stress. Turkish Journal of Biology. 43 (1): 58-69.
Bharadwaj, N., Gogoi, N., Barthakur, S. and Basumatary, N. (2018). Morphophysiological responses in different mungbean genotypes under drought stress. Research Journal Recent Science. 7 (7): 1-5.
Caverzan, A., Casassola, A. and Brammer, S. P. (2016). Reactive oxygen species and antioxidant enzymes involved in plant tolerance to stress. SHANKER AK & SHANKER C. Abiotic and biotic stress in plants-Recent advances and future perspectives. Publisher InTech. 463-480.
Chagas, R. Silveira, J. Ribeiro, R. Vitorello, V. and Ca-rrer, H. (2008). Photochemical damage and comparative performance of superoxide dismutase and ascorbate pe-roxidase in sugarcane leaves exposed to paraquatindu-ced oxidative stress. iolgy Journal. (90):181-188.
Chance, B. and Maehly, A.C. (1955). Assay of catalases and peroxidases. Methods in Enzymologist. (11): 764 - 755.
Chang, C. J. and Kao, C. H. (1998). H2O2 metabolism during senescence of rice leaves: changes in enzyme activities in light and darkness. Plant Growth Regulation Journal. 25. (1): 11-15.
Dolatabadian, A., Sanavy, S. M. and Chashmi, N. A. (2008). The effects of foliar application of ascorbic acid (vitamin C) on antioxidant enzymes activities, lipid peroxidation and proline accumulation of canola (Brassica napus L.) under conditions of salt stress. Journal of Agronomy and Crop Science. 194. (3): 206-213.
El-Beltagi, H. S., Mohamed, H. I. and Sofy, M. R. (2020). Role of Ascorbic acid, Glutathione and Proline Applied as Singly or in Sequence Combination in Improving Chickpea Plant through Physiological Change and Antioxidant Defense under Different Levels of Irrigation Intervals. Molecules Journal. 25. (7): 1702.
Farooq, A., Bukhari, S. A., Akram, N. A., Ashraf, M., Wijaya, L., Alyemeni, M. N. and Ahmad, P. (2020). Exogenously Applied Ascorbic Acid-Mediated Changes in Osmoprotection and Oxidative Defense System Enhanced Water Stress Tolerance in Different Cultivars of Safflower (Carthamus tinctorious L.). Plants Journal. 9. (1): 92-104.
Fahad, S., Bajwa, A. A., Nazir, U., Anjum, S. A., Farooq, A., Zohaib, A. and Ihsan, M. Z. (2017). Crop production under drought and heat stress: plant responses and management options. Frontiers in plant science Journal. (8):, 23-47.
Giannopolitis, C. N. and Rie, S. K. (1977). Superoxide dismutases. I. Occurrence in higher plants. Plant Physiology. (59): 309 - 314.
Gunes, A. Cicek, N. Inal, A. Alpaslan, M. Eraslan, F. Guneri, E. and Guzelordu, T. (2006). Genotypic response of chickpea (Cicer arietinum L.) cultivars to drought stress implemented at pre-and postanthesis stages and its relations with nutrient uptake and efficiency. Journal of Plant Soil Environment. (52): 868-876.
Helal, R. M. and Samir, M. A. (2008). Comprative response of drought tolorant and drought sensitive maize genotypes to water stress. Australian Journal crops science. (1): 31-36.
Lu, Y., Chang, X. and Guo, X. (2019). Dynamic changes of ascorbic acid, phenolics biosynthesis and antioxidant activities in mung beans (Vigna radiata L.) until maturation. Plants, 8(3), 75.
Mercado, J. A. Matas, A. J. Heredia, A. Valpuesta, V. and Quesada, M. (2004). Changes in the water binding characteristics of the cell walls from transgenic Nicotiana tabacum leaves with enhanced levels of peroxidase activity. Plant Physiology. (122): 504-512.
Mittler, R. Vanderauwera, S. Gollery, M. and Breusegem, F. V. (2004). Reactive oxygen gene network of plants. Trends in Plant Science. (9): 490-498.
Nakano, Y. and Asad, K. (1981). Hydrogen peroxide is scavenged by ascorbate peroxidase in spinach chloroplasts. Plant and Cell Physiology. (22): 867–880.
Nasr Esfahani, M. (2013). Effect of water-deficit Stress on Growth and Antioxidant System in Three Chickpea Cultivars. Herbal Biology. 5. (15): 111-124.
Nickel, K. S. and Cunningham, B. A. (1969). Improved peroxidase assay method using leuco 2, 3′, 6-trichloroindophenol and application to comparative measurements of peroxidatic catalysis. Analytical Biochemistry. 27. (2): 292-299.
Niu, L. and Liao, W. (2016). Hydrogen peroxide signaling in plant development and abiotic responses: crosstalk with nitric oxide and calcium. Frontiers in Plant Science. (7): 230-245.
Noctor, G. and Foyer, C. H. (1998). Ascorbate and glutathione: Keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology. (49): 249-279.
Rahal, A. Kumar, A. Singh, V. Yadav, B. Tiwari, R. Chakraborty, S. and Dhama, K. (2014). Oxidative stress, prooxidants, and antioxidants: the interplay. BioMed Research International.
Sajid, Z. A. and Aftab, F. (2009). Amelioration of salinity tolerance in Solanum tuberosum L. by exogenous application of ascorbic acid. In Vitro Cellular & Developmental Biology-Plant. 45. (5): 529- 540.
Samota, M. K., Sasi, M., Awana, M., Yadav, O. P., Amitha Mithra, S. V., Tyagi, A., Kumar, S. & Singh, A. (2017). Elicitor-induced biochemical and molecular manifestations to improve drought tolerance in rice (Oryza sativa L.) through seed-priming. Frontiers in plant science. (8): 911-934.
Singh, R., Singh, S., Parihar, P., Mishra, R. K., Tripathi, D. K., Singh, V. P., Devendra, K. C. and Prasad, S. M. (2016). Reactive oxygen species (ROS): beneficial companions of plants’ developmental processes. Frontiers in plant science (7): 84-99.
Swathi, L., Reddy, D. M., Sudhakar, P. and Vineela, V. (2017). Screening of Mung bean (Vigna radiata L. Wilczek) genotypes against water stress mediated through polyethylene glycol. International Journal Curr Microbiol Application Science. 6. (10): 2524-2531.
Sayfzadeh, S. and Rashidi, M. (2011). Response of antioxidant enzymes activities of sugarbeet to drought stress. ARPN Journal of Agricultural and Biological Science. 6. (4): 27-33.
Seyed Ebrahimi, F. S. Hasani Komala, H. Alamali, A. and Rezaodoost, M. H. (2015). Effect of water-deficit stress on morphological traits and activity of antioxidant enzymes of Brassica Napus. Process and Plant Yield. 4. (14): 77-91.
Simova-Stoilova, L. Demirevska, K. Petrova, T. Tsenov, N. and Feller, U. (2008). Antioxidative protection in wheat varieties under severe recoverable drought at seedling stage. Plant Soil Environment. (54): 529-536.
Singh, N. and Bhardwaj, R. D. (2016). Ascorbic acid alleviates water deficit induced growth inhibition in wheat seedlings by modulating levels of endogenous antioxidants. Biologial science. 71. (4): 402-413.
Singh, R., Singh, M. K., Singh, A. K. and Singh, C. (2018). Pulses production in India: Issues and elucidations.
Tohidi-Moghaddam, H. R. Shirani-Rad, A. R. Noormohammadi, G. Habibi, D. and Boojar, M. M. A. (2009). Effect of super absorbent application on antioxidant enzyme activities in canola (Brassica napus L.) cultivars under water stress conditions. American Journal of Agriculture and Biological Science. (4): 215-223.
Upadhyaya, H. and Panda, S. K. (2004). Responses of Camellia sinensis to drought and rehydration. Biologia Plantarum. 48. (4): 597-600.
Venkatesh, J. and Park, S. W. (2014). Role of L-ascorbate in alleviating abiotic stresses in crop plants. Botanical studies. 55. (1): 21-38.
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Abdul Jaleel, C. Riadh, K. Gopi, R. Manivannan, P. Ines, J. Al-Juburi, H. J. Chang-Xing, Z. Hong-Bo, S. and Panneerselvam, R. (2009). Antioxidant defense responses: physiological plasticity in higher plants under abiotic constrains. Acta Physiologia Plantarum 31: 427-436.
Afkari, A. (2017). Effect of seed priming on germination characteristics and some antioxidant enzymes activity of Basil (Ocimum basilicum L.) under drought stress conditions. Journal of Developmental Biology. 9)3:(33-44.
Ahmad, P. Jaleel, C.A. Salem, M. A. Nabi, G. and Sharma, S. (2010). Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Critical Reviews in Biotechnology. 30 (3):161-175.
Ahmed, S. Nawata, E. Hosokawa, M. Domae, Y. and Sakuratani, T. (2002). Alterations in photosynthesis and some antioxidant enzymatic activity of mung bean subjected to waterlogging. Journal of Plant Science. 163: 117-123.
Akram, N. A., Shafiq, F. and Ashraf, M. (2017). Ascorbic acid-a potential oxidant scavenger and its role in plant development and abiotic stress tolerance. Frontiers in plant science. (8): 601-613.
Alderfasi, A. A., Alzarqaa, A. A., Al-Yahya, F. A., Roushdy, S. S., Dawabah, A. A. and Alhammad, B. A. (2017). Effect of combined biotic and abiotic stress on some physiological aspects and antioxidant enzymatic activity in mungbean (Vigna radiate L.). Africal Journal Agricalture Research. 12 (9): 700-705.
Allen, R. G., Pereira, L. S., Raes, D., and Smith, M. (1998). Crop evapotranspiration-Guidelines for computing crop water requirements-FAO Irrigation and drainage paper 56. Fao, Rome, 300. (9). D05109.
Amini, F. Askari, M. and Haghir, M. (2017). Changes in protein and antioxidant system of Cucumis sativus cv. Isfahani in response to drought stress. Journal of Cell & Tissue (JCT). 7(4): 375-386.
Arab, P. Bradaran Firouzabadi, M. Asghari, H. Gholami, A. and Rahimi, M. (2011). The effect of ascorbic acid and sodium nitroposide spraying on some safflower characteristics under irrigation tension. Proceedings of the 12th Iranian Congress of Plant Breeding. Islamic Azad University. Karaj Branch. Karaj. 16-14.
Aydinsakir, K., Erdal, S., Buyuktas, D., Bastug, R. and Toker, R. (2013). The influence of regular deficit irrigation applications on water use, yield, and quality components of two corn (Zea mays L.) genotypes. Agricultural water management. (128): 65-71.
Basu, S., Ramegowda, V., Kumar, A. and Pereira, A. (2016). Plant adaptation to drought stress. F1000 Research, 5.
Bangar, P., Chaudhury, A., Tiwari, B., Kumar, S., Kumari, R. and Bhat, K. V. (2019). Morphophysiological and biochemical response of mung bean [Vigna radiata (L.) Wilczek] varieties at different developmental stages under drought stress. Turkish Journal of Biology. 43 (1): 58-69.
Bharadwaj, N., Gogoi, N., Barthakur, S. and Basumatary, N. (2018). Morphophysiological responses in different mungbean genotypes under drought stress. Research Journal Recent Science. 7 (7): 1-5.
Caverzan, A., Casassola, A. and Brammer, S. P. (2016). Reactive oxygen species and antioxidant enzymes involved in plant tolerance to stress. SHANKER AK & SHANKER C. Abiotic and biotic stress in plants-Recent advances and future perspectives. Publisher InTech. 463-480.
Chagas, R. Silveira, J. Ribeiro, R. Vitorello, V. and Ca-rrer, H. (2008). Photochemical damage and comparative performance of superoxide dismutase and ascorbate pe-roxidase in sugarcane leaves exposed to paraquatindu-ced oxidative stress. iolgy Journal. (90):181-188.
Chance, B. and Maehly, A.C. (1955). Assay of catalases and peroxidases. Methods in Enzymologist. (11): 764 - 755.
Chang, C. J. and Kao, C. H. (1998). H2O2 metabolism during senescence of rice leaves: changes in enzyme activities in light and darkness. Plant Growth Regulation Journal. 25. (1): 11-15.
Dolatabadian, A., Sanavy, S. M. and Chashmi, N. A. (2008). The effects of foliar application of ascorbic acid (vitamin C) on antioxidant enzymes activities, lipid peroxidation and proline accumulation of canola (Brassica napus L.) under conditions of salt stress. Journal of Agronomy and Crop Science. 194. (3): 206-213.
El-Beltagi, H. S., Mohamed, H. I. and Sofy, M. R. (2020). Role of Ascorbic acid, Glutathione and Proline Applied as Singly or in Sequence Combination in Improving Chickpea Plant through Physiological Change and Antioxidant Defense under Different Levels of Irrigation Intervals. Molecules Journal. 25. (7): 1702.
Farooq, A., Bukhari, S. A., Akram, N. A., Ashraf, M., Wijaya, L., Alyemeni, M. N. and Ahmad, P. (2020). Exogenously Applied Ascorbic Acid-Mediated Changes in Osmoprotection and Oxidative Defense System Enhanced Water Stress Tolerance in Different Cultivars of Safflower (Carthamus tinctorious L.). Plants Journal. 9. (1): 92-104.
Fahad, S., Bajwa, A. A., Nazir, U., Anjum, S. A., Farooq, A., Zohaib, A. and Ihsan, M. Z. (2017). Crop production under drought and heat stress: plant responses and management options. Frontiers in plant science Journal. (8):, 23-47.
Giannopolitis, C. N. and Rie, S. K. (1977). Superoxide dismutases. I. Occurrence in higher plants. Plant Physiology. (59): 309 - 314.
Gunes, A. Cicek, N. Inal, A. Alpaslan, M. Eraslan, F. Guneri, E. and Guzelordu, T. (2006). Genotypic response of chickpea (Cicer arietinum L.) cultivars to drought stress implemented at pre-and postanthesis stages and its relations with nutrient uptake and efficiency. Journal of Plant Soil Environment. (52): 868-876.
Helal, R. M. and Samir, M. A. (2008). Comprative response of drought tolorant and drought sensitive maize genotypes to water stress. Australian Journal crops science. (1): 31-36.
Lu, Y., Chang, X. and Guo, X. (2019). Dynamic changes of ascorbic acid, phenolics biosynthesis and antioxidant activities in mung beans (Vigna radiata L.) until maturation. Plants, 8(3), 75.
Mercado, J. A. Matas, A. J. Heredia, A. Valpuesta, V. and Quesada, M. (2004). Changes in the water binding characteristics of the cell walls from transgenic Nicotiana tabacum leaves with enhanced levels of peroxidase activity. Plant Physiology. (122): 504-512.
Mittler, R. Vanderauwera, S. Gollery, M. and Breusegem, F. V. (2004). Reactive oxygen gene network of plants. Trends in Plant Science. (9): 490-498.
Nakano, Y. and Asad, K. (1981). Hydrogen peroxide is scavenged by ascorbate peroxidase in spinach chloroplasts. Plant and Cell Physiology. (22): 867–880.
Nasr Esfahani, M. (2013). Effect of water-deficit Stress on Growth and Antioxidant System in Three Chickpea Cultivars. Herbal Biology. 5. (15): 111-124.
Nickel, K. S. and Cunningham, B. A. (1969). Improved peroxidase assay method using leuco 2, 3′, 6-trichloroindophenol and application to comparative measurements of peroxidatic catalysis. Analytical Biochemistry. 27. (2): 292-299.
Niu, L. and Liao, W. (2016). Hydrogen peroxide signaling in plant development and abiotic responses: crosstalk with nitric oxide and calcium. Frontiers in Plant Science. (7): 230-245.
Noctor, G. and Foyer, C. H. (1998). Ascorbate and glutathione: Keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology. (49): 249-279.
Rahal, A. Kumar, A. Singh, V. Yadav, B. Tiwari, R. Chakraborty, S. and Dhama, K. (2014). Oxidative stress, prooxidants, and antioxidants: the interplay. BioMed Research International.
Sajid, Z. A. and Aftab, F. (2009). Amelioration of salinity tolerance in Solanum tuberosum L. by exogenous application of ascorbic acid. In Vitro Cellular & Developmental Biology-Plant. 45. (5): 529- 540.
Samota, M. K., Sasi, M., Awana, M., Yadav, O. P., Amitha Mithra, S. V., Tyagi, A., Kumar, S. & Singh, A. (2017). Elicitor-induced biochemical and molecular manifestations to improve drought tolerance in rice (Oryza sativa L.) through seed-priming. Frontiers in plant science. (8): 911-934.
Singh, R., Singh, S., Parihar, P., Mishra, R. K., Tripathi, D. K., Singh, V. P., Devendra, K. C. and Prasad, S. M. (2016). Reactive oxygen species (ROS): beneficial companions of plants’ developmental processes. Frontiers in plant science (7): 84-99.
Swathi, L., Reddy, D. M., Sudhakar, P. and Vineela, V. (2017). Screening of Mung bean (Vigna radiata L. Wilczek) genotypes against water stress mediated through polyethylene glycol. International Journal Curr Microbiol Application Science. 6. (10): 2524-2531.
Sayfzadeh, S. and Rashidi, M. (2011). Response of antioxidant enzymes activities of sugarbeet to drought stress. ARPN Journal of Agricultural and Biological Science. 6. (4): 27-33.
Seyed Ebrahimi, F. S. Hasani Komala, H. Alamali, A. and Rezaodoost, M. H. (2015). Effect of water-deficit stress on morphological traits and activity of antioxidant enzymes of Brassica Napus. Process and Plant Yield. 4. (14): 77-91.
Simova-Stoilova, L. Demirevska, K. Petrova, T. Tsenov, N. and Feller, U. (2008). Antioxidative protection in wheat varieties under severe recoverable drought at seedling stage. Plant Soil Environment. (54): 529-536.
Singh, N. and Bhardwaj, R. D. (2016). Ascorbic acid alleviates water deficit induced growth inhibition in wheat seedlings by modulating levels of endogenous antioxidants. Biologial science. 71. (4): 402-413.
Singh, R., Singh, M. K., Singh, A. K. and Singh, C. (2018). Pulses production in India: Issues and elucidations.
Tohidi-Moghaddam, H. R. Shirani-Rad, A. R. Noormohammadi, G. Habibi, D. and Boojar, M. M. A. (2009). Effect of super absorbent application on antioxidant enzyme activities in canola (Brassica napus L.) cultivars under water stress conditions. American Journal of Agriculture and Biological Science. (4): 215-223.
Upadhyaya, H. and Panda, S. K. (2004). Responses of Camellia sinensis to drought and rehydration. Biologia Plantarum. 48. (4): 597-600.
Venkatesh, J. and Park, S. W. (2014). Role of L-ascorbate in alleviating abiotic stresses in crop plants. Botanical studies. 55. (1): 21-38.
