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Open Access Article
1 - Fabrication of Au/ZnO/MWCNTs electrode and its characterization for electrochemical cholesterol biosensor
Davood Ghanei Agh Kaariz Elham Darabi Seyed Mohammad Elahi -
Open Access Article
2 - Designing Competitive Nanobiosensor for Ochratoxin based on FRET using quantum dot
Mozaffari, M., Bayat, M., Mohsenifar, A., Hashemi, S.J. .In this research, using FRET (fluorescence resonance energy transfer) from Cd/Te quantum dot (anti Ochratoxin A antibody immobilized on the external surface of quantum dot or QDs) to (Ochratoxin A labeled with Rhodamine 123 bound to albumin), a sensitive competitive imm MoreIn this research, using FRET (fluorescence resonance energy transfer) from Cd/Te quantum dot (anti Ochratoxin A antibody immobilized on the external surface of quantum dot or QDs) to (Ochratoxin A labeled with Rhodamine 123 bound to albumin), a sensitive competitive immunoassay was developed for measuring Ochratoxin A. The highly specific immune-reaction between anti-Ochratoxin A antibody on the QDs and labeled Ochratoxin A brings the Rho fluorophore and the QDs in close spatial proximity, and following photo-excitation of the QDs, causes FRET to occur between the Rho fluorophore (acting as the acceptor) and the QDs (acting as the donor). In the absence of free Ochratoxin A, the immune reaction between labeled Ochratoxin and the anti-Ochratoxin antibody on the QDs induces emission and causes FRET to occur. In the presence of free Ochratoxin A, it competes with the labeled Ochratoxin A-albumin complex for binding to the antibody-QDs conjugate in the Nanobiosensor, leading to reduction in Rhodamine emission after FRET. The reduction in the fluorescence intensity of the Rhodamine acceptor directly correlates with the concentration of free Ochratoxin A in the sample. This method has a detection limit of 220pg per ml. It has also been used to measure Ochratoxin A in human serum samples. A linear relationship is found between the increase in the fluorescence intensity of Rho 123 at580 nm and the concentration of OTA in spiked samples over the 100-800 pg⋅mL−1 concentration range. This highly sensitive homogeneous competitive detection scheme is simple, rapid and efficient. It does not require multiple separation steps and excessive washing. Manuscript profile -
Open Access Article
3 - Design of a biosensor using antibodies immobilized on the quantum dots and rhodamine attached to citrinin to detect mycotoxin
بهروز Shojaee Sadi, Mansour Bayat , P Tajik SJ HashemiCitrinine is a nephrotoxic metabolite that damages the kidneys in animals. Therefore, the development of detoxification methods during the food process is important. Quantum devices, using a single source of excitation, are excited by individual wavelengths and produce MoreCitrinine is a nephrotoxic metabolite that damages the kidneys in animals. Therefore, the development of detoxification methods during the food process is important. Quantum devices, using a single source of excitation, are excited by individual wavelengths and produce different emission peaks. In this study, a new bioconjugation-based sensor consisting of quantum dot semiconductor nanocrystals and anti-citrinin antibody was used to determine the amount of citrinin toxins using the FRET (Fluorescence Resonance Energy Transfer) system. Semiconductor nanocrystals or quantum materials (CdTe, CdTe / CdS) were synthesized in the laboratory using reflux method by reducing tellurium powder under a stream of nitrogen gas. The fluorescence intensity of quantum data was determined using a spectrofluorimetric device. Antibody activity in nanobioconjugate was also measured by spectrofluorimetry. The citrinin-albumin conjugate was then attached to the fluorescence compound rhodamine 123, which is a highly affinity immunological reaction between the anti-citrinin-quantum antibody (donor) and the citrinin-rhodamine 123 (receptor) label used as fluorophora. After adding the nanobioconjugated solution to the cuvette, which contains a certain amount of phosphate buffer, citrinin was added to it in different concentrations, and after a few minutes, changes in the intensity of quantum dot fluorescence were observed. A regular decrease in fluorescence intensity was obtained by increasing the citrinin concentration. Due to the collective effects of mycotoxins on human and animal health, it is better to determine the extent of food contamination with mycotoxins, including citrinin, using nanobiosensors as a specific method that results from the use of monoclonal antibodies. Unlike conventional methods such as ELISA and HPLC, these designed nanobiosensors are homogeneous, simple, fast, and inexpensive and do not require much separation or rinsing. Manuscript profile -
Open Access Article
4 - Assimilation of Biosensor by Means of Microcontact Scheme Based on Molecularly Imprinted Polymer for Detection of Salmonella Typhi
S.A.A. Anvar V. Razavilar B. Akbari Adergani A. Motallebi Moghangoghi H. AhariIntroduction: The detection of the bacteria mass in food products using traditional methods is a long process that might take four to ten days. The advances in nano technology has made the design of selective and intelligent sensors possible that are capable of precisel MoreIntroduction: The detection of the bacteria mass in food products using traditional methods is a long process that might take four to ten days. The advances in nano technology has made the design of selective and intelligent sensors possible that are capable of precisely detecting the bacteria mass in a short time. Materials and Methods: In this research, the MIP (molecularly imprinted polymer) was utilized. First, in order to prepare the molecular imprints and produce the polymer, meta-acrylic acid (MAA) monomers were used. Then, covalence bonds between the MAA monomers created a white polymer and hydrogen bonds between the antibody and MAA were formed. By using fluorescence converter and its connection to the antibody of Salmonella Typhi bacteria, it is possible to detect the Salmonella Typhi anti-gene. The florescence disseminations after neighbouring were quantified by a spectrofluorometer. Results: The results indicated that, it is possible to detect the concentration of Salmonella Typhi bacteria with a minimum mass of 10 Mol in polluted waters. Moreover, the designed sensor was evaluated in terms of configuration specificity. Also, the Ecoli bacteria were inseminated in an aquatic environment containing Salmonella Typhi proving that there was no interference with the sensor function. Besides, sensor sensitivity was examined up to 60 days and showed that sensor performance could be verified up to 52 days and then starts to diminish. Conclusion: The applications of biosensors to detect microbial contaminations have been increased due to the shortening of detection time while retaining the required characteristics and sensitivity. Manuscript profile -
Open Access Article
5 - Designing of Molecular Framework Polymer Nanobiosensor Based on Potentiometric Method for Staphylococcus aureus Exotoxin Detection
Hamed Ahari Vadood Razavilar Behrooz Akbari Abbas Ali MotallebiIntroduction: Considering the ever increasing population and industrialization of the developmental trend of humankind's life, one is hardly able to detect the toxins produced in food products using the traditional techniques. This is due to the fact that the quality su MoreIntroduction: Considering the ever increasing population and industrialization of the developmental trend of humankind's life, one is hardly able to detect the toxins produced in food products using the traditional techniques. This is due to the fact that the quality survey for food products is not cost-effective and even in most of the cases, the precision in the practical techniques like the bacterial cultivation and other techniques suffer from laboratories errors. Hence with the advent of nanotechnology, the design of selective and smart sensors is one of the greatest transformation of the quality control of food products that in few minutes time, and with a very high precision one can identify the toxin level of bacteria. In the present research, the use of the molecular framework polymer of bacterial toxin as the modifier for the improvement of electrochemical properties of PVC film electrode in order to measure the exotoxin is experienced.Materials and Methods: In this technique, the production of molecular framework and polymer is done using meta acrylic acid monomers, that are formed via covalence connection between meta acrylic acid monomers (MAA) of white polymer. Here also hydrogenic connection between exotoxin amino acid and meta MAA is made that would function as the selective absorption for that.Result: The results indicate that the molecular framework polymer sensor is capable of detecting up to the density of 10-3. Additionally, the sensitivity of the sensors were examined up to 60 days and was confirmed for 28 days and then started to decrease.Conclusion: Although the sensitivity of the technique was low but detection precision was perfect. To increase the sensitivity of the test, it is planned to design another technique to increase the sensitivity of the test. Manuscript profile -
Open Access Article
6 - The use of Biological Indicators as Biosensors
Maryam Mohammadi Roozbahani Nasrin choobkarEcological indicators can be used to assess the condition of the environment, to provide an early warning signal of changes in the environment, or to diagnose the cause of an environmental problem. Ideally the suite of indicators should represent key information about s MoreEcological indicators can be used to assess the condition of the environment, to provide an early warning signal of changes in the environment, or to diagnose the cause of an environmental problem. Ideally the suite of indicators should represent key information about structure, function, and composition of the ecological system. There are some concerns that hamper the use of ecological indicators as a resource management tool. Monitoring programs often depend on a small number of indicators and fail to consider the full complexity of the ecological system. Choice of ecological indicators is confounded in management programs that have vague long-term goals and objectives. Management and monitoring programs often lack scientificrigor because of their failure to use a defined protocol for identifying ecological indicators. Thus, ecological indicators need to capture the complexities of the ecosystem yet remain simple enough to be easily and routinely monitored. Ecological indicators should meet the following criteria: be easily measured, be sensitive to stresses on the system, respond to stress in a predictable manner, be anticipatory, predict changes that can be averted by management actions, be integrative, have a known response to disturbances, anthropogenic stresses, and changes over time, and have low variability in response. It is better than using the GIS system for the best analysis to select the indicators. Manuscript profile -
Open Access Article
7 - Biosensors Application in Analysis of Environmental contaminants from Agricultural Industries
Marzieh Hosseini Nezhad Saeed SamadiWater contamination and environmental pollution is considered as a main concern in global healthissues. Considering the large number of chemical and microbial contaminants released to theenvironment, in particular those from agricultural origin, effective tools of detec MoreWater contamination and environmental pollution is considered as a main concern in global healthissues. Considering the large number of chemical and microbial contaminants released to theenvironment, in particular those from agricultural origin, effective tools of detection are required forprevention of challenges involved in health and safety. Biosensors offer rapid and effective detectionoptions to control biological hazards. These are recognized as beneficial devices in providing accurate,sensitive and rapid analytical results in quantitative and qualitative detection of pollutants. This paperis summarizes the advances in development and application of biosensors for environmental analysisand quantification of contaminants initiated from agricultural procedures Manuscript profile -
Open Access Article
8 - Investigation of environmental contamination caused by organophosphate pesticides by acetylcholinesterase biosensor
Ali Shamsazar Fatemeh Shamsazar Asadollah AsadiPesticides are chemical substances that use to control insects, fungis, weeds and other pests. Residuals of pesticides maybe enter to food chain through air, water and soil and cause health problems for ecosystems, birds, animals and humans. Common methods to detect pes MorePesticides are chemical substances that use to control insects, fungis, weeds and other pests. Residuals of pesticides maybe enter to food chain through air, water and soil and cause health problems for ecosystems, birds, animals and humans. Common methods to detect pesticides including, high performance liquid chromatography (HPLC) and gas chromatography (GC). But these methods are time consuming and require a technician to control, so use of biosensor can be useful in this field. An acetylcholinesterase biosensor was used to detection of organophosphate pesticides (case study: Paraoxon). Qualitative identification of pesticides based on inhibition of the acetylcholinesterase enzyme and reduce activity of it in the face of pesticides. Also Other parameters were investigated such as pH, concentration of pesticides and sustainability of biosensor also. The designed biosensor showed high sensitivity to paraoxon concentration. Under optimal condition, inhibition of acetylcholinesterase by paraoxon had a linear relation with increasing concentrations of the paraoxon in the range of 10-7 to 10-4 mM. Also prepared sensor showed good stability. The designed acetylcholinesterase enzymatic biosensor with 2.14 × 10-7 mM identification limit can be use as a sensitive and accurate detector in order to rapid identification pesticides in contaminated environment such as soil and water, in industry and agriculture sectors. Manuscript profile -
Open Access Article
9 - DNA Biosensor for Determination of 5-Fluorouracil based on Gold Electrode Modified with Au and Polyaniline Nanoparticles and FFT Square Wave Voltammetry
Parviz Norouzi Mohammad Amin Eshraghi Mehrnaz Ebrahimi -
Open Access Article
10 - The Effect of Carbon Nanotube on Improving Catalytic G-quadruplex Sensing Properties
Fahimeh Otovat Mohammad Reza Bozorgmehr Ali Mahmoudi Ali Morsali -
Open Access Article
11 - Synthesis of Alaninemethylester and N-(4-aminobenzyl)-9Hpurin-6-amine Doped to Polyaniline Nanoparticles and Study on Their Interactions with Ds-DNA by Fluorescence Spectroscopy
Banafsheh Gorji Reza Zadmard Kian Tavakol Fatemeh Shahkarami -
Open Access Article
12 - effect of G-quadruplex on aromaticity of phenanthroline based ligands: mlecular dynamics simulation and density functional theory methods
Mohadese Bazobandi Mohammad Reza Bozorgmehr Ali Mahmoudi Ali MorsaliG-quadruplexes are biostructures that are used as biomarkers. These compounds are composed of four guanine sheets. Studying the interaction of G-quadruplex with planar ligands is very important. In this work, the effect of G-quadruplex on the aromaticity of three phenan MoreG-quadruplexes are biostructures that are used as biomarkers. These compounds are composed of four guanine sheets. Studying the interaction of G-quadruplex with planar ligands is very important. In this work, the effect of G-quadruplex on the aromaticity of three phenanthroline based ligands was studied by molecular dynamics simulation and density functional theory. The investigated ligands are: N,N'-(dibenzo [b,j] [1,7]phenanthroline-2,10-diylbis (methylene)) die-thanamine (L1), N,N'-(dibenzo [b,j] [1,7] phenanthroline-2,10diylbis (methylene)) bis (N-methylpropan-1-amine) (L2) and N1-(dibenzo [b, j] [1,7] phenanthroline-7-ylmethyl)-N3 -methy lpropane-1,3-diamine (L3) which have 5 aromatic rings in their structure. To check the effect of temperature, calculations were repeated at four temperatures of 20, 40, 60 and 80 degrees Celsius. The HOMA index was calculated as an index of the aromaticity of the rings. The obtained results show that the L1 ligand has higher aromaticity than the other two ligands. Also, as the temperature increases, the aromaticity of the ligand rings decreases. On the other hand, the terminal two rings of the ligands are more affected by G-quadruplex. In general, the aromaticity of the five-membered rings of the ligands increases with the interaction with the G-quadruplex. Manuscript profile -
Open Access Article
13 - Studying the π-π interaction between single-stranded oligonucleotides and single-walled carbon nanotubes by molecular dynamics simulation and density functional theory
Mohammad Reza Bozorgmehr Maryam Ghanbari Ghanbarloo Ali MorsaliCarbon nanotubes are used in smart drug delivery due to their biocompatibility. However, due to the non-polar nature of these compounds, their use in the in vivo environment is limited. To solve this limitation, functionalization of nanotubes is used. Biological compoun MoreCarbon nanotubes are used in smart drug delivery due to their biocompatibility. However, due to the non-polar nature of these compounds, their use in the in vivo environment is limited. To solve this limitation, functionalization of nanotubes is used. Biological compounds such as nucleic acids are used to functionalize nanotubes. Understanding the interaction mechanism of nanotubes with biological compounds can help in smart drug delivery. In this research, the interaction of four homocopolymers of organic bases adenine, guanine, cytosine and thymine with carbon nanotubes was studied by molecular dynamics simulation and density functional theory. The distance between the two ends of each homocopolymer and the number of atoms of the organic base rings of the homocopolymers were calculated. These two quantities were used to calculate the probability of the system being in different states. Then, the Gibbs free energy for each interaction was calculated using the calculated probabilities. The results show that purine homocopolymers surround the carbon nanotube, while pyrimidine homocopolymers are placed on the surface of the nanotube. The number of monomers with π-π interaction in homocopolymer with guanine 4, adenine 3, cytosine and thymine 2 was obtained. Manuscript profile -
Open Access Article
14 - Control-ability of refractive index sensor based on Plasmonic Induced Transparency in Graphene meta-material
Hossein Fazlali pour Seyed Hadi Fatemi Nasab -
Open Access Article
15 - Design sensitive plasmonic based 1D photonic crystal biosensor with graphene-two metal layer
Maryam Sharifi Sadeq Mohammed Ameen Mahmood -
Open Access Article
16 - Designing real-time biosensors and chemical sensors based on symmetrical photonic crystal heterostructures
maryam sharifi Habib Tajalli Hamid Pashaei Adl Payman Tajalli -
Open Access Article
17 - 1D Photonic Crystal Based-Biosensor for Multiple Biomarkers Detection
Farzaneh Bayat Kazem Jamshidi-Ghaleh -
Open Access Article
18 - The Study of Plasmonic Biosensors based on Mach-Zehnder Interferometry
Reza Kheradmand Tanin Kazemzadeh Fariba Lotfi -
Open Access Article
19 - 1D Photonic Crystal-Based Biosensor for Multiple Biomarkers Detection
Farzaneh Bayat Kazem Jamshidi-Ghaleh -
Open Access Article
20 - A Biosensor for the Detection of Type b3a2 Related to Chronic Myelogenous Leukemia by Using Locked Nucleic Acid, Molecular Switching, Magnetic Nanoparticles, and Enzymatic Signal Amplification
Hamzeh Amoshahi Mohammad Reza Mohammad Shafiee Shabnam Kermani Mehrosadat Mirmohammadi -
Open Access Article
21 - Performance Investigation of Pentacene Based Organic Double Gate Field Effect Transistor and its Application as an Ultrasensitive Biosensor
Mohammad Reza Jouharchi Zahra Ahangari Farshad Babazadeh -
Open Access Article
22 - Performance Investigation of Pentacene Based Organic Double Gate Field Effect Transistor and its Application as an Ultrasensitive Biosensor
Mohammad Reza Jouharchi zahra ahangari Farshad Babazadeh -
Open Access Article
23 - Fabrication of choline oxidase enzyme-based nanobiosensor for the detection of diazinon and comparing its performance with the high performance liquid chromatography
اقدس بنائی ناهید پارسافر جلیل بدراقیOrganophosphorus compounds enter the environment naturally or after human use as pesticides or chemical fertilizers, and ultimately enter the food chain of organisms. Due to their undesirable effects, it is absolutely necessary to detect these toxins at very low concent MoreOrganophosphorus compounds enter the environment naturally or after human use as pesticides or chemical fertilizers, and ultimately enter the food chain of organisms. Due to their undesirable effects, it is absolutely necessary to detect these toxins at very low concentrations before they enter the life cycle. Biosensors are suitable tools for detecting these toxins. In this study, a mono-enzyme biosensor based on inhibiting the cholineoxidase enzyme with its immobilization on a modified electrode with carboxylate carbon nanotubes was fabricated and in the presence of 1mM enzyme substrate )choline chloride(, the minimum detection limit for diazinon was obtained (0.56 μM( and two linear ranges (1.5- 2.4 μM and 5.5-17.5 μM( was observed. The detection of low concentrations of diazinon by this biosensor and high performance liquid chromatography )HPLC( were compared. The result of the biosensor had 3.5% error relative to HPLC Manuscript profile -
Open Access Article
24 - Detection of Escherichia coli in pharmaceutical and water samples using a biosensor based on carbon nanotubes containing gold nanoparticles
Fatemeh Behoftadeh Mohammad Faezi Ghasemi Ali Mojtahedi khosro Issazadeh Mostafa GolshekanBackground & Objectives: Escherichia coli is an important indicator in the quality control of pharmaceutical and real samples. This study compares the detection of this bacterium by regular method and a biosensor based on Multi-Walled Carbon Nanotubes (MWCNTs) on glassy MoreBackground & Objectives: Escherichia coli is an important indicator in the quality control of pharmaceutical and real samples. This study compares the detection of this bacterium by regular method and a biosensor based on Multi-Walled Carbon Nanotubes (MWCNTs) on glassy carbon electrodes (GCE) in pharmaceutical and water as real sample. Materials and Methods: In this experimental study, the conventional culture method (pour plate) and modified biosensor based on Multi-Walled Carbon Nanotubes on glassy carbon electrode with the arrangement of GC/MWCNTs/AuNPs/Ab/BSA were used for the detection of E. coli. Dilutions of E. coli between (1 ×101–1×108 CFU/ml) were used in pharmaceutical and water samples, prepared in 0.1 M PBS (pH 7.4), mixed with 0.5mM acetaminophen. The efficiency of the designed biosensor was investigated using SEM, Cyclic Voltammetry, and Square-Wave Voltammetry electrochemical techniques, as well as interfering bacteria. Results: The results of E. coli detection using the conventional culture and designed biosensor were not statistically significant. The designed biosensor had a high sensitivity with accuracy in 3 minutes and LOD 3.02 CFU/ml for Escherichia coli. Conclusion: Considering the time-consuming and influenced by environmental factors in the microbial monitoring of pharmaceuticals for E. coli detection in conventional methods and the risk of losing pharmaceutical products, the biosensor has good efficiency in detection with low cost and no need for enrichment in a small volume of samples. Manuscript profile -
Open Access Article
25 - The role of nanobiosensors in identifying pathogens and environmental hazards
Ramin Mohammadi Aloucheh Yousef Alaee Mollabashi Asadollah Asadi Ozlem baris Somayeh Gholamzadeh -
Open Access Article
26 - Thermodynamic Analysis of Short Single-Stranded DNA (ssDNA) for Advancing DNA-Based Biosensor-biocatalyst Development
Mohammad Reza Bozorgmehr Maryam Ghanbari-Ghanbarlo Ali MorsaliThis study aimed to enhance our understanding of short single-stranded DNA (ssDNA) to facilitate the development of novel DNA-based biosensors-biocatalyst. A 10-base ssDNA model was constructed based on the 130-145 codon sequence of the p53 gene, a key tumor suppressor MoreThis study aimed to enhance our understanding of short single-stranded DNA (ssDNA) to facilitate the development of novel DNA-based biosensors-biocatalyst. A 10-base ssDNA model was constructed based on the 130-145 codon sequence of the p53 gene, a key tumor suppressor gene. By employing molecular dynamics (MD) simulations, we delved into the thermodynamic properties and equilibrium states of the ssDNA system, unveiling crucial insights into its behavior. Various macroscopic observables were investigated during the MD simulations, including temperature, energy distributions, and the root mean square deviation (RMSD) of the ssDNA's nucleic acid backbone. The structural model of the ssDNA was meticulously constructed using the AMBER program, ensuring accuracy and reliability. Subsequently, atomistic MD simulations were conducted in three different ensembles utilizing the Gromacs program. The microcanonical, canonical, and isobaric-isothermal ensembles were employed to compare and contrast the equilibrium characteristics of the ssDNA in aqueous solutions. The choice of ensemble played a decisive role in capturing the dynamic equilibrium and conformational behavior of the ssDNA system. The distribution of energy, encompassing both kinetic and potential energy, provided valuable insights into the establishment of thermodynamic equilibrium. Fluctuations in temperature and total energy underscored the finite nature of the system, while the average kinetic energy confirmed the attainment of physiological temperature. Furthermore, the RMSD analysis shed light on the conformational stability of the ssDNA, with both NVT and NPT ensembles exhibiting stable conformational states under their respective thermodynamic conditions. These findings emphasize the intricate interplay between thermodynamic conditions and the conformational flexibility of ssDNA. Manuscript profile -
Open Access Article
27 - Exploring the Interaction of Oligonucleotides with Single-Walled Carbon Nanotube as industrial bio-catalysis: A Molecular Dynamics Simulation Study
Mohammad Reza Bozorgmehr Maryam Ghanbari-Ghanbarlo Ali MorsaliIn this research, the interaction of four single stranded nucleic acid homopolymers including hemo deca adenine, dA10, hemo deca thymine, dT10, hemo deca guanine, dG10 and hemo deca cytosine, dC10, with single-walled carbon nanotubes was studied by molecular dynamics si MoreIn this research, the interaction of four single stranded nucleic acid homopolymers including hemo deca adenine, dA10, hemo deca thymine, dT10, hemo deca guanine, dG10 and hemo deca cytosine, dC10, with single-walled carbon nanotubes was studied by molecular dynamics simulation method. The simulations were performed using Gromacs software and Amber force field, with a simulation time of 250 nanoseconds and 2 femtosecond time step. The root mean square deviation (RMSD) values were calculated to validate the simulations, indicating that the systems reached equilibrium. The distance between the center of mass of the homo deca polymers and the surface of the carbon nanotube was also calculated, and the results showed that the interaction of monocyclic organic bases with the nanotubes was higher due to less steric hindrance with the phosphate group. The results suggest that monocyclic organic bases may be more suitable for interactions with carbon nanotubes due to their lower steric hindrance. Manuscript profile