Designing of Molecular Framework Polymer Nanobiosensor Based on Potentiometric Method for Staphylococcus aureus Exotoxin Detection

Subject Areas : Microbiology

Hamed Ahari ¹ , Vadood Razavilar ² , Behrooz Akbari ³ , Abbas Ali Motallebi ⁴

1 - مربی دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، دانشکده علوم و مهندسی صنایع غذایی، تهران، ایران
2 - استاد دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، دانشکده علوم تخصصی دامپزشکی، تهران، ایران
3 - استاد دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، دانشکده علوم تخصصی دامپزشکی، تهران، ایران
4 - دانشیار مرکز تحقیقات آزمایشگاهی غذا و دارو، سازمان غذا و دارو، وزارت بهداشت، درمان و آموزش پزشکی، تهران، ایران

Received: 2012-11-26 Accepted : 2012-05-01 Published : 2013-12-22

Keywords: Molecular Framework Polymer se, Nanobiosensor, S.aureusExotoxin,

Abstract :

Introduction: Considering the ever increasing population and industrialization of the developmental trend of humankind's life, one is hardly able to detect the toxins produced in food products using the traditional techniques. This is due to the fact that the quality survey for food products is not cost-effective and even in most of the cases, the precision in the practical techniques like the bacterial cultivation and other techniques suffer from laboratories errors. Hence with the advent of nanotechnology, the design of selective and smart sensors is one of the greatest transformation of the quality control of food products that in few minutes time, and with a very high precision one can identify the toxin level of bacteria. In the present research, the use of the molecular framework polymer of bacterial toxin as the modifier for the improvement of electrochemical properties of PVC film electrode in order to measure the exotoxin is experienced.Materials and Methods: In this technique, the production of molecular framework and polymer is done using meta acrylic acid monomers, that are formed via covalence connection between meta acrylic acid monomers (MAA) of white polymer. Here also hydrogenic connection between exotoxin amino acid and meta MAA is made that would function as the selective absorption for that.Result: The results indicate that the molecular framework polymer sensor is capable of detecting up to the density of 10-3. Additionally, the sensitivity of the sensors were examined up to 60 days and was confirmed for 28 days and then started to decrease.Conclusion: Although the sensitivity of the technique was low but detection precision was perfect. To increase the sensitivity of the test, it is planned to design another technique to increase the sensitivity of the test.

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