Assimilation of Biosensor by Means of Microcontact Scheme Based on Molecularly Imprinted Polymer for Detection of Salmonella Typhi

Subject Areas : Microbiology

S.A.A. Anvar ¹ , V. Razavilar ² , B. Akbari Adergani ³ , A. Motallebi Moghangoghi ⁴ , H. Ahari ⁵

1 - استادیارگروه بهداشت، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
2 - استاد گروه بهداشت، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
3 - دانشیار مرکز تحقیقات آزمایشگاهی غذا و دارو، سازمان غذا و دارو، وزارت بهداشت، درمان و آموزش پزشکی، تهران، ایران
4 - استاد گروه بهداشت، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
5 - استادیار گروه علوم و صنایع غذایی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران

Received: 2016-05-14 Accepted : 2016-05-14 Published : 2016-05-21

Keywords: Assimilation, Biosensor, Microcontact, Salmonella Typhi,

Abstract :

Introduction: The detection of the bacteria mass in food products using traditional methods is a long process that might take four to ten days. The advances in nano technology has made the design of selective and intelligent sensors possible that are capable of precisely detecting the bacteria mass in a short time. Materials and Methods: In this research, the MIP (molecularly imprinted polymer) was utilized. First, in order to prepare the molecular imprints and produce the polymer, meta-acrylic acid (MAA) monomers were used. Then, covalence bonds between the MAA monomers created a white polymer and hydrogen bonds between the antibody and MAA were formed. By using fluorescence converter and its connection to the antibody of Salmonella Typhi bacteria, it is possible to detect the Salmonella Typhi anti-gene. The florescence disseminations after neighbouring were quantified by a spectrofluorometer. Results: The results indicated that, it is possible to detect the concentration of Salmonella Typhi bacteria with a minimum mass of 10 Mol in polluted waters. Moreover, the designed sensor was evaluated in terms of configuration specificity. Also, the Ecoli bacteria were inseminated in an aquatic environment containing Salmonella Typhi proving that there was no interference with the sensor function. Besides, sensor sensitivity was examined up to 60 days and showed that sensor performance could be verified up to 52 days and then starts to diminish. Conclusion: The applications of biosensors to detect microbial contaminations have been increased due to the shortening of detection time while retaining the required characteristics and sensitivity.