Fusarium wilt

Open Access Article

1 - Greenhouse and molecular evaluation of resistance of Iranian bean cultivars to Fusarium oxysporum f.sp. phaseoli, the causal agent of Fusarium wilt
Ehsan Hasanvand seddiqe mohammadi

Fusarium wilt (yellow) that caused by the fungus Fusarium oxysporum f. sp. phaseoli, is an important disease and can cause severe damage to bean products world wide and cause reduced functional. Since farming techniques to control the disease is not completely effective More

Fusarium wilt (yellow) that caused by the fungus Fusarium oxysporum f. sp. phaseoli, is an important disease and can cause severe damage to bean products world wide and cause reduced functional. Since farming techniques to control the disease is not completely effective, cultivars with genetic resistance to the disease are recommended. In order to identify resistant cultivars experiment in that randomized complete block design with three replications and 12 trkeatments, including different cultivars of beans. By method at a concentration suspension inoculation of root drench were inoculated. In root drench method suspension spores of pathogen because of healthy roots a lower percentage of spores could penetrating in roots. So it was far less severe symptoms and can say all cultivars has same statistical group and were resistant and infected cultivars tolerant. Considering the fact that the disease causes yellowing and wilting the factors concentration of chlorophyll a, chlorophyll b, and carotenoid concentrations and chlorophyll total concentrations were measured with evaluating resistant and tolerant cultivars were identified. Accordingly, cultivars Sayad and E9 respectively than other cultivars showed more resistant to the disease. Then the reaction is different bean cultivars to fungal F. oxysporum f.sp. phaseoli were evaluated using specific marker SCAR. According to cultivars Akhtar, Naz, Sadri, Sayad, E9 and WA with primer pairs SU20 have a band of 750 bp and A55 are resistant gene but cultivars Talash, Shekofa, Jegari, Aje, Khomeyn and capsules with specific primer pairs SU20 did not produce band. Manuscript profile

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2 - Evaluation of biocontrol potential of some Trichoderma and Bacillus species against the causal agent of Fusarium wilt of melons
Zaynolabedin Nurozei Kamran Rahnama Hojjatollah Rabbani nasab Misam Taqinasab

Three isolates of Trichoderma spp. and two isolates of bacillus bacterial were evaluated in biological control of Fusarium oxysporum f. sp melonis the laboratory and greenhouse condition. Dual culture, antimicrobial metabolites, and vo More

Three isolates of Trichoderma spp. and two isolates of bacillus bacterial were evaluated in biological control of Fusarium oxysporum f. sp melonis the laboratory and greenhouse condition. Dual culture, antimicrobial metabolites, and volatile metabolites were in invitro assay. Fungal pathogen colony area was recorded, compared with control and induced inhibition of growth was determined. The experiment displayed the antagonistic ability using dual culture simultaneous and dual culture un-simultaneous assays against fungal pathogen. Results showed that Trichoderma harzianum 6 whit 70% and T. viride10 whit 52.9 highest percentage of growth inhibition. Results of dual culture highest percentage of growth inhibition about 30.5 percent of isolates whit the Bacillus spp62 percent growth inhibit the growth factor. The volatile compounds Bacillus spp15 whit 12.37 percent of the precipitation was prevented from growing. In the treatment Bacillus spp62 and mixed two spice tin treatments at Trichoderma 14g1 respectively 84.93 and 84.95 were showed most effects on length growth of stem but most effects of increased fresh weight from up ground organ of plant, was mixed treatment as spices T. longibrachiatum and bacteria Bacillus spp.15. Most effects on root fresh weight were showed by Trichoderma harzianum. Most effect on stem dry weight T. viridae with T. harzianum. Most effects on increased dry weight were showed by Bacillus spp.15and T. harzianum. Results of the experiment show treatments have significantly different activity pathogen. Test average correspond were meant significant at 1% level by LSD test. Manuscript profile

Open Access Article

3 - Susceptibility assessment of potato cultivars to Fusarium wilts
Mehdi Nasresfahani Rasoul Sharifi Narges Nasrollahi Shima Ramezani

Fusarium wilt (Fusariumoxysporumf.sp. tuberose) is a major disease of potato, causing considerable damage and losses. The sources of genetically resistant are the best method of controlling the disease. In this study, the reactions of 18 potato cultivars wereassessed to More

Fusarium wilt (Fusariumoxysporumf.sp. tuberose) is a major disease of potato, causing considerable damage and losses. The sources of genetically resistant are the best method of controlling the disease. In this study, the reactions of 18 potato cultivars wereassessed to F. o f.sp. tuberosiin a factorial design with four replicates, in the greenhouse.Plants were inoculated (15 cm height) twice with three isolates of the pathogen by root inoculation (100 ml of 4×105 spore/ ml).Tento thirty days after inoculation cultivars were evaluated according to the symptoms disease index, the lengths of necrosis in vascular tissues, stem height and roots lengthsThere was a significant difference between parameters(P= 0.01). Necrosis lengths had maximum correlation in vascular tissues (r = 0.86), then root lengths(r = 0.75), symptom disease index scale (r = 0.73), and stem heights(r = 0.69). There was a significant difference between cultivars(P= 0.01). According to lengths of necrosis in vascular tissues cultivars divided in five groups, Gorican, Tiuva, Diamont, Marfona, Baraka,Aola and Moren were very susceptible and Olva, Folva and Alva were tolerant.  Manuscript profile

Open Access Article

4 - In vivo biological activity of Piriformospora indica, Sebacina vermifera and Trichoderma spp. against Fusarium wilt of lentil
Hossein Kari Dolatabadi Ebrahim Mohammadi Goltapeh

Lentil Fusarium wilts, caused by Fusarium oxysporum f. sp. lentis (Vasd. and Srin.) Gordon is one of the most important factors of reducing lentil yield in the world. Effect of four soil borne fungi Piriformospora indica, Sebacina vermifera, Trichoderma harzianum and Tr More

Lentil Fusarium wilts, caused by Fusarium oxysporum f. sp. lentis (Vasd. and Srin.) Gordon is one of the most important factors of reducing lentil yield in the world. Effect of four soil borne fungi Piriformospora indica, Sebacina vermifera, Trichoderma harzianum and Trichoderma viride on Fusarium wilt oflentilwere evaluated in a completely randomized design under greenhouse condition. Pathogen was inoculated to soil pots three times (10 days before sowing, while sowing and 10 days after sowing). Seventeen treatments including two controls (plant without pathogen and plant with pathogen) and fifteen combinations of above antagonistic fungi were inoculated while sowing. Different growth factors namely: plant heights, root length, dry weight of shoot and root and disease severity were assessed in flowering stage. Results revealed that the most effect of combination of antagonistic fungi was observed in pots inoculated with treatment (S. vermifera + T. harzianum). Manuscript profile

Open Access Article

5 - Antagonistic effects of Trichoderma spp. against Fusarium oxysporum f.sp. lycopersici causal agent of tomato wilt
Hesamadin Ramezani

In this study, the mycoparasitism inhibitory effects of five Trichoderma species (T. harzianum, T. koningi, T. longiconis, T. hamatum and T. viride) on the growth of the causal agent of tomato Fusarium wilt (Fusarium oxysporum f.sp. lycopersici) were investigated by dua More

In this study, the mycoparasitism inhibitory effects of five Trichoderma species (T. harzianum, T. koningi, T. longiconis, T. hamatum and T. viride) on the growth of the causal agent of tomato Fusarium wilt (Fusarium oxysporum f.sp. lycopersici) were investigated by dual culture in laboratory condition. In this step, the maximum and minimum inhibitory effect was caused by T. harzianum and T. viride. In the greenhouse, the comparison of the efficacy of disease decrease was carried out between soil and seed treatments affected by T. harzianum spores. Results showed that seed treatment did not cause disease decrease but soil treatment caused disease decrease by 92%.  Manuscript profile

Open Access Article

6 - Efficacy of neem products compare with bavistin for management of Fusarium wilt of chickpea
Hesamedin Ramezani Sasan Ghasemi

A field trial was conducted to find out the efficacy of neem based [neem seed water extracts (NSWE) @ 40 ml/lit and Vanguard @ 5 ml/lit], comparison with bavistin @ 1.5 g/lit, against the Fusarium wilt of chickpea (Fusarium oxysporum f.sp. ciceri). Bavistin proved to be More

A field trial was conducted to find out the efficacy of neem based [neem seed water extracts (NSWE) @ 40 ml/lit and Vanguard @ 5 ml/lit], comparison with bavistin @ 1.5 g/lit, against the Fusarium wilt of chickpea (Fusarium oxysporum f.sp. ciceri). Bavistin proved to be the most effective against the Fusarium wilt between these treatments. Maximum plant height was recorded in case of plot treated with Vanguard followed by seed water extract. Maximum number of pods was collected from plots sprayed with Vanguard. Highest grain yield was obtained due to bavistin, applied at 30, 50 and 70 days after sowing (DAS) but the benefit/cost ratio was highest (3.03:1) in one spray of NSWE, applied at 70 DAS which was more economical than other treatments.  Manuscript profile

Open Access Article

7 - Study of interaction between plant nutrition and cucumber Fusarium wilt disease caused by Fusarium oxysporum f.sp. cucumerinum
Parvin Younesi Hasan Reza Etebarian Ali Mohammad Roustaee

Cucumber wilt disease caused by Fusarium oxysporum f.sp. cucumerinum  is one of the most important diseases of cucumber. Disease severity can be decreased by plant nutrition management, especially nitrogen level. In this study, the effects of nitrogen sources such More

Cucumber wilt disease caused by Fusarium oxysporum f.sp. cucumerinum  is one of the most important diseases of cucumber. Disease severity can be decreased by plant nutrition management, especially nitrogen level. In this study, the effects of nitrogen sources such as KNO3 and NH4NO3 on the growth of fungus (Fusarium oxysporum f. sp. cucumerinum) on PDA medium and  interaction between plant nutrition (N) and cucumber Fusarium wiltdisease on some cultivar of cucumber in greenhouse hydroponic culture were investigated. The effects of nitrogen sources such as KNO3 and NH4NO3 were investigated on growth area of F.oxysporum f. sp. cucumerinum on PDA medium by a completely randomized design with 4 replications. Variance analysis of growth area of the fungus showed significant effect on the different factors used in this experiment. The highest area growth of fungus was obtained at 0.1 g/l for KNO3 . Ammonium nitrate (NH4NO3) significantly reduced the mycelial growth compared to the control treatment. Effects of different N levels were investigated on two greenhouse cucumber cultivars (exterem and soltan) at the presence of fungus (F. oxysporum f. sp. cucumerinum). A completely randomized design with 4 replications was used for this experiment. The results showed that increasing of nitrogen level (20 meq/l NO3-) compared to the control treatment (distilled water + fungus) decrease the disease severity. The results showed that cucumber nutrition with nitrogen affects the F. oxysporum f. sp. cucumerinum. Also the importance of plant nutrituin management for decrease of disease severity was shown. Manuscript profile

Open Access Article

8 - Preparation of Talaromyces flavus liquid and microcapsule formulations and their application in commercial tomato greenhouses to increase yield
Laleh Naraghi Seyed Reza Fani Sadegh Jalali Maryam Negahban Shahram Naeimi

With the recent advances in the application of biotechnology in various sciences, the preparation of liquid bio-formulations and microcapsule suspension/powder from the important antagonistic fungal agent Talaromyces flavus has been investigated in recent years. In the More

With the recent advances in the application of biotechnology in various sciences, the preparation of liquid bio-formulations and microcapsule suspension/powder from the important antagonistic fungal agent Talaromyces flavus has been investigated in recent years. In the first year of this research, a type of liquid bioformulation, a type of microcapsule suspension, and a type of microcapsule powder were prepared for use in tomato greenhouses. During one year, commercial greenhouses were investigated in tomato greenhouses in two regions of Yazd and Isfahan with a history of Fusarium wilt disease. The treatments in each study of the commercial greenhouse were 1-3) each of the liquid formulations, microcapsule suspension, and microcapsule powder by soil application, 4-6) each of the liquid formulations, microcapsule suspension, and microcapsule powder with the tomato seedling root dip, 7) Talaromin fungicide by soil application, and 8) a control (without any formulation and fungicide application). The results indicated that all three formulations in both application methods (soil application or seedling root dip and seed impregnation for tomatoes and cucumbers, respectively) significantly increased the yield of tomatoes compared to the control. Altogether, microcapsule powder with seed impregnation and liquid formulation with soil application were the most effective treatments with approximately 50% and 60% increases in yield compared to the control, respectively, for the management of tomato Fusarium wilt disease. According to the obtained results, the production method of these formulations is considered technical knowledge, and it is possible to carry out their commercialization steps. Manuscript profile

Open Access Article

9 - Study on the infections status of Fusarium wilt disease of tobacco in Golestan province
Seyed Afshin Sajjadi

Fusarium oxysporum f. sp. nicotianae, the causal agent of tobacco fusarium wilt, is one of the important agents that distributed worldwide and cause yield losses in tobacco growing countries. Control of this pathogen are accomplished through the use of chemical control, More

Fusarium oxysporum f. sp. nicotianae, the causal agent of tobacco fusarium wilt, is one of the important agents that distributed worldwide and cause yield losses in tobacco growing countries. Control of this pathogen are accomplished through the use of chemical control, biological control, use of plant extracts, resistant cultivars and etc. The objective of this study was to evaluation of infections status of this disease in the tobacco fields of Golestan province. 45 tobacco fields in the five different villages of Gorgan region (Valeshabad, Taghartappeh, Jafarabad, Nodehmalek and Ghorogh) and the four different villages of Aliabad region (Baraftan, Pichakmahaleh, Elazman and Fazelabad) were selected in 2016 year. From the beginning of disease symptoms appearance, weekly surveys were done from fields and amount of disease incidence percent was recorded during the infection period. Statistical analysis were done using Stat Graphics Centurion XVI software. The results of this research showed that the based on the highest disease incidence there were no significant differences between regions, but there were significant differences (P<0.001) among fields. One field with disease incidence 48.2% in Valeshabad was highest disease incidence and combined Valeshabad with disease incidence 31.16% had the highest infection and Taghartappeh with disease incidence 18.5% had the lowest infection. Based on areas under disease progress curves, there no significant differences between regions, but there were significant differences (P<0.001) between fields. One field with under disease progress curves 1816.2 in Valeshabad had highest. In combined Valeshabad with 2700 had highest disease progress curves and Taghartappeh with 971.8 disease progress curves had the lowest. The results of this study show that Fusarium wilt disease of tobacco in all regions in Golestan province (Gorgan and Aliabad) with different rate of expansion. Manuscript profile

Open Access Article

10 - Study of effect of methanol extracts of Garlic, Cinnamon and liquid smoke from tobacco waste on Inhibitionof Tobacco Fusarium wiltof pathogen fungi
seyd afshin sajadi

Tobacco Fusarium wilts of pathogen fungi are important phytopathogens distributed worldwide and can cause yield losses in tobacco growing countries. The management of fungal soilborne pathogens are accomplished through the use of pesticides, resistant varieties, biologi More

Tobacco Fusarium wilts of pathogen fungi are important phytopathogens distributed worldwide and can cause yield losses in tobacco growing countries. The management of fungal soilborne pathogens are accomplished through the use of pesticides, resistant varieties, biological control, and use of plant extracts is done. The use of plant extracts for management of this disease is preferable because pesticides are expensive and may pollute the environment. This design investigated the inhibitory effects of some medicinal plant extracts on growth of Fusarium oxysporum f. sp. nicotianae the cause of tobacco collar rot and selection of a suitable concentration is performed in the laboratory in Tirtash Research and Education Center in 2018. The plants (Garlic and Cinnamon) were extracted with methanol and liquid smoke used in this study was obtained from the pyrolysis of tobacco waste. Tobacco waste was pyrolyzed in furnace in the absence of oxygen and smoke was converted to liquid smoke with cold water. This study were carried out in factorial with three factors including: crude extracts (Garlic, Cinnamon and Tobacco waste) and 6 concentration (0, 250, 500, 1000, 2000 and 4000 ppm) based on Completely Randomized Design with 3 replications. The minimum inhibitory concentration of each extracts was determined by agar diffusion method. Results indicated that crude extracts of Garlic, Cinnamon and Tobacco waste (liquid smoke) have remarkable antifungal activity. With increasing concentrations of plant extracts, inhibitory effect on mycelium growth of fungal F. o f. sp. nicotianae increased. The minimum inhibitory concentration of liquid smoke of tobacco waste and methanol extracts of Garlic and Cinnamon on tobacco Fusarium wilt of against fungiwere equal to 2000, 4000 and 4000 ppm, respectively. The liquid smoke of tobacco waste at concentrations greater than 2000 ppm has fungicide and methanol extracts of Garlic and Cinnamon at concentrations greater than 4000 ppm has fungistate.   Manuscript profile

Open Access Article

11 - Evaluation of Trichoderma spp. isolates for biocontrol of fusarium wilt of carnation in greenhouse
Asma Moeeni Mohammad Torabi Dariush Shahriari

One of the most destructive soil-borne diseases of carnation is fusarium wilt caused by Fusarium oxysporumf.sp. dianthi. In This study, antagonistic potential of different isolotes of three Trichoderma species on growth of the pathogen in laboratory and control of the d More

One of the most destructive soil-borne diseases of carnation is fusarium wilt caused by Fusarium oxysporumf.sp. dianthi. In This study, antagonistic potential of different isolotes of three Trichoderma species on growth of the pathogen in laboratory and control of the disease in greenhouse was investigated. Isolates of the antagonist were Ta1, Ta2 and Ta3 belonging to Trichoderma atroviride, sixteen isolates Th1-Th16 belonging to T. harzianum and one isolate Tl1 belonging to T. longibrachiatum. Dual culture and colonization tests were carried out in a completely randomized design with three replications. Isolates  Ta3, Ta2 and Ta1 which completely covered colonies of the pathogen after five days, were the mosts successful colonizers. Inhibitory effect of volatile metabolites of Ta3 isolate in 72h and 96h cultures of the pathogen and antagonis (60% and 72%, respectively) was higher than those of the other isolates. In greenhouse, soil treatment with isolates of Trichoderma, to study the biocontrol effects the isolates was carried out in an experiment with 21 treatments in three replications. The results showed that different isolates could control the disease in different levels. Isolate Ta3 had the highest effect on disease control and reduction of cuttings death, followed by isolates Ta1, Ta2, Ta3 and Th2. In general, the antagonist isolates had less effects on growth of the plant, but reduced disease severity.   Manuscript profile

Open Access Article

12 - Low magnetic field effect with magnetic water on the control of Fusarium wilting of seedlings of tomatoes
Arash Momeni Saghar Ketabchi

Tomato Fusarium wilt caused by Fusarium oxysporum f. sp. Lycopersici is one of the most important diseases of tomato in Iran and also around the world. Magnetic fields can be considered as an environmental factor that can have a significant effect on the performance and More

Tomato Fusarium wilt caused by Fusarium oxysporum f. sp. Lycopersici is one of the most important diseases of tomato in Iran and also around the world. Magnetic fields can be considered as an environmental factor that can have a significant effect on the performance and growth of plants. The interaction between the magnetic field and the time of seed exposure in the field has been extremely effective in promoting plant growth characteristics. A similar phenomenon of resonance increases domestic seeds’ energy, if the right combination of magnetic field and the time of exposure are applied which would lead to higher yield level. North Pole causes higher plant growth and South Pole prevents it. In this study, a 3-D magnetizer X.Y.Z. device (such as a MRI medical device with non-hazardous wavelength) was applied to make tomato seeds and seedlings magnetic in varied treatments. Among the treatments, the best result was achieved by using the combination of immersed seeds in magnetized water 0.7M through the magnetic field 0.4mT for 60 minutes. Manuscript profile

Open Access Article

13 - Assessment of tomato genotypes resistance to Verticillium and Fusarium wilt diseases using molecular markers
Bahareh Morid Shahab Haj Mansour

Background & Objectives: Fusarium and Verticillium wilts are two major fungal wilt diseases of tomato that have restricted its production worldwide. Culturing resistant tomato cultivars is the best way to control such diseases. Molecular markers linked to resistance More

Background & Objectives: Fusarium and Verticillium wilts are two major fungal wilt diseases of tomato that have restricted its production worldwide. Culturing resistant tomato cultivars is the best way to control such diseases. Molecular markers linked to resistance genes would be useful for improving tomato breeding programs. In this study allele, specific markers and cleaved amplified polymorphic sequences (CAPS) markers were used to identify tomato genotypes that are resistant to Verticillium wilt and Fusarium wilt, respectively. Materials & Methods: This cross-sectional study was carried out on 32 tomato hybrids and commercial varieties provided from Falat company. DNA was extracted using cetyl-trimethyl ammonium bromide (CTAB) method. Then, polymerase chain reaction (PCR) was performed using molecular markers. To detect Fusarium wilt resistant varieties, PCR-RFLP was down using RsaI and FokI restriction enzymes. The results were confirmed by pathogenicity test. Results: Out of 35 tomato genotypes, 83% were resistant to Verticillium wilt, while 17% were sensitive. Also, 46% of genotypes were resistant to Fusarium wilt, while 54% were sensitive. Genotypes that showed resistance to Verticillium and Fusarium wilts possessed Ve1 and I-2 genes, respectively. Conclusion: Planting resistant genotypes in infected areas can control fungal diseases such as Verticillium and Fusarium wilts, without using any fungicides. Manuscript profile

Open Access Article

14 - Identification of resistant eggplant cultivars to Fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt using molecular markers in Iran
Negin Safikhani Bahar Morid Hamid reza Zamanizadeh Shahab Hajmansoor

Background & Objectives: Eggplant fusarium wiltis an important factor of yield reduction throughout the world. The ability of this pathogen to survive for several consecutive years within the soil, even in the absence of the host, has made it difficult to control. P More

Background & Objectives: Eggplant fusarium wiltis an important factor of yield reduction throughout the world. The ability of this pathogen to survive for several consecutive years within the soil, even in the absence of the host, has made it difficult to control. Producing and using the resistant cultivar is the most effective and suitable method to control this disease. This study was aimed to identify fusarium wilt resistant eggplant cultivars.   Materials & Methods: First, leaf samples of domestic and hybrid eggplant cultivars were gathered from 28 provinces in Iran and then DNA extraction from young leaves of the cultivars was carried out using CTAB method. Four markers including CAPS, RAPD, SRAP, and SCAR were used to determine the resistant cultivars. In order to confirm the results, resistance and sensitivity of the genotypes were assessed in greenhouse conditions, as well.   Results: Out of 20 genotypes of this study, 13  showed index resistance band using CAPS, RAPD, and SRAP of molecular markers. On the other hand, the SCAR marker could not separate the resistant cultivars from the sensitive ones. Phenotype assessment of native and hybrids resistant cultivars in greenhouse condition confirmed the results of the molecular analysis.   Conclusion: In general, the use of resistant cultivars obtained in this study using molecular markers is recommended for planting in areas with fusarium wilt disease. Manuscript profile

Open Access Article

15 - Application of Soil‐borne Streptomycetes for Biological Control against Fusarium Wilt of Cumin (Cuminum cyminum L) caused by Fusarium oxysporum fsp cumini
Mozhgan Golmoradi Gholamhosein Shahidi Bonjar Sonia Aghighi Meysam Soltani Nejad

10.30495/jmw.2021.690439

Cumin )C uminum cyminum L.) is an annual plant belonging to Apiaceae family. One of the major diseases of cumin is Fusarium wilt caused by a soil-borne, vascular wilt pathogen Fusarium oxysporum f.sp. cumini which is a devastating disease that occurs in major cumin grow More

Cumin )C uminum cyminum L.) is an annual plant belonging to Apiaceae family. One of the major diseases of cumin is Fusarium wilt caused by a soil-borne, vascular wilt pathogen Fusarium oxysporum f.sp. cumini which is a devastating disease that occurs in major cumin growing areas of the world; while plants reach to 0.5- 2.5 cm in height, they die as the result of the disease. In the current research, 80 isolates of Streptomyces spp. isolated from the rhizosphere soil of Astragalus sp. and screened against Fusarium under laboratory condition. Three out of 80 isolates including M15, M26 and M80 revealed having the highest antagonistic activity, hence, selected for further evaluation under greenhouse conditions. After emergence of cumin seedlings, mortality and growth indices were compared between different treatments weekly and results were recorded. Plants were harvested after teen weeks and growth indices such as plant height and weight were recorded. The Streptomyces sp. Isolate No. M15 was showed the strongest effects on plant growth and suppression of the wilt disease as compared to the controls. The present research is an attempt to control cumin Fusarium wilt disease using Streptomyces spp. The final goal of this research is to introduce an effective biological agent for controlling managing Fusarium wilt disease under field condition. this pathogen. Manuscript profile

Open Access Article

16 - Identification of resistant genes linked to fusarium wilt disease in tomato (Lycopersicon esculentum) cultivars using CAPS markers
Bahar Morid Shahab Haj Mansoor

    Fusarium wilt causes cosiderable yield loss in most tomato-growing regions around the world. The best way to control this disease is using tomato resistant cultivars. Molecular markers linked to resistance gene would be useful for tomato improvement p More

    Fusarium wilt causes cosiderable yield loss in most tomato-growing regions around the world. The best way to control this disease is using tomato resistant cultivars. Molecular markers linked to resistance gene would be useful for tomato improvement programs. In this study, a cleaved amplified polymorphic sequence (CAPS) marker (TAO1902) was used to identify tomato cultivars possessing I-2 gene, which confers resistance to Fusarium oxysporum f. sp. lycopersici race 2. DNA was extracted using CTAB method from 27 hybrids and commercial tomato varieties. To evaluate existence of I-2 gene in tomato cultivars, PCR-RFLP was down using RsaI and FokI restriction enzymes. An RsaI-digested fragment of 500 bp and two restriction fragments of 390 and 410 bp for FokI digestion of TAO1902 were revealed in the resistant varieties. Results showed that 14 out of 27 tomato hybrids and cultivars were homozygous resistant and the other 13 hybrids were susceptible to fusarium wilt.  Pathogenicity test showed that there were no symptoms in resistance gene I-2 amplifing tomato varieties. However, tomato varieties with no resistance band produced disease symptoms with different disease intensities. Results showed that 7 out of 27 tomato cultivars and hybrids, and 7 hybrids were resistant to F. oxysporum f. sp. lycopersici race 2 and so, these cultivars and hybrids are recommended in regions that race 2 is dominant. Manuscript profile

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