Preparation of Talaromyces flavus liquid and microcapsule formulations and their application in commercial tomato greenhouses to increase yield
Subject Areas : Agriculture Marketing and CommercializationLaleh Naraghi 1 , Seyed Reza Fani 2 , Sadegh Jalali 3 , Maryam Negahban 4 , Shahram Naeimi 5
1 - PhD student of Public Administration - Human Resource Management, Islamic Azad University, Kerman, Iran
2 - Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
3 - Plant Protection Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
4 - Department of Pesticides Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
5 - Department of Biological Control Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
Keywords: Biological control, Cucumber, Fusarium wilt, Talaromyces flavus, Tomato.,
Abstract :
With the recent advances in the application of biotechnology in various sciences, the preparation of liquid bio-formulations and microcapsule suspension/powder from the important antagonistic fungal agent Talaromyces flavus has been investigated in recent years. In the first year of this research, a type of liquid bioformulation, a type of microcapsule suspension, and a type of microcapsule powder were prepared for use in tomato greenhouses. During one year, commercial greenhouses were investigated in tomato greenhouses in two regions of Yazd and Isfahan with a history of Fusarium wilt disease. The treatments in each study of the commercial greenhouse were 1-3) each of the liquid formulations, microcapsule suspension, and microcapsule powder by soil application, 4-6) each of the liquid formulations, microcapsule suspension, and microcapsule powder with the tomato seedling root dip, 7) Talaromin fungicide by soil application, and 8) a control (without any formulation and fungicide application). The results indicated that all three formulations in both application methods (soil application or seedling root dip and seed impregnation for tomatoes and cucumbers, respectively) significantly increased the yield of tomatoes compared to the control. Altogether, microcapsule powder with seed impregnation and liquid formulation with soil application were the most effective treatments with approximately 50% and 60% increases in yield compared to the control, respectively, for the management of tomato Fusarium wilt disease. According to the obtained results, the production method of these formulations is considered technical knowledge, and it is possible to carry out their commercialization steps.
_ Alimi T. & Ajewole OC. & Olubode-Awosola OO. & Idowu EO. (2006). Economic rationale of commercial organic fertilizer technology in vegetable production in Osun State of Nigeria. Journal of Applied Horticulture, 8(2):159-164.
_ Cruz DR. & Leandro LFS. & Munkvold GP. (2019). Effects of temperature and pH on Fusarium oxysporum and soybean seedling disease, Plant disease, 103(12):1-9.
_ Farhang Niya S. & Naraghi L. & Ommati F. & Pirnia M. (2015). Evaluation of the efficacy of the biological compound affected by Talaromyces flavus in controlling tomato Fusarium wilt disease in the field conditions. International Journal of Agricultural Science and Research, 5(2):153-164.
_ Guan H. & Chi D. & Yu J. & Li X. (2008). A novel photodegradable insecticide: Preparation characterization and properties evaluation of nano-imidacloprid. Pesticide Biochemistry and Physiology, 2(1):83-91.
_ Husen E. & Simanungkalit RDM. & Suraswati R. & Irawan I. (2007). Characterization and quality assessment of Indonesian commercial biofertilizer. Indonesian Journal of Agricultural Science, 8(1):31-38.
_ Kaewchai S. & Soytong K. & Heydari KD. (2009). Mycofungicides and fungal biofertilizers. Fungal Diversity, 38(1):25-50.
_ Kah M. & Hofmann T. (2014). Nanopesticide research: current trends and future priorities. Environment international, 63(1):224-235.
_ Kim KK. & Fravel DR. & Papavizas GC. (1990). Production, purification and properties of glucose oxidase from the biocontrol fungus Talaromyces flavus. Canadian Journal of Microbiology, 36(3):1-9.
_ Kohl J. & Kolnaar R. & Raversberg WJ. (2019). Mode of action of microbial control agents against plant diseases: Relevance beyond efficacy. Frontiers in Plant Science, 10(845): 2-14.
_ Lee J. & Choi JY. & Park CH. (2008). Characteristics of polymers enabling nano– commoinution of water–insoluble drugs. International Journal of pharmaceutics, 55(1):328-336.
_ Liu L. & Kloepper JW. & Tuzun S. (1995). Induction of systemic resistance in cucumber against Fusarium wilt by plant growth promoting rhizobacteria. Phytopathology 85(1):695- 698.
_ Lymperopoulos P. & Msanne J. & Rabara R. (2018). Phytochrome and phytohormones working in tandem for plant growth and development. Frontiers in Plant Science, 9(1):2-14.
_ Maji R. & Dey N. & Satapathy B. & Mukherjee B. & Mondal S. (2014). Preparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy. International journal of nanomedicine, 9(1):3107-3114.
_ Manoch L. & Dethoup T. (2011). A potential Talaromyces species and biological agents against plant pathogenic fungi. Thai Journal of Agricultural Science, 44(2):81-91.
_ Martin A. & Varona S. & Navarrete A. & Cocero M. (2010). Encapsulation and co-precipitation processes with supercritical fluids: applications with essential oils. The Open Chemical Engineering Journal, 4(1):31-41.
_ Naraghi L. & Naeimi S. & Marzban R. & Heydari A. (2020). A study on the development of Talaromyces flavus formulations by a fermenter and some of their biological properties. The Journal of Research on the Lepidoptera, 51(1):74-92.
_ Naraghi L. & Naeimi S. & Marzban R. (2019a). Application of Talaromyces flavus formulations repared by a fermenter for controlling sugar beet seedling damping-off disease in the greenhouse conditions. Academia Journal of Agricultural Research, 7(11):274-281.
_ Naraghi L. & Naeimi S. & Marzban R. (2019b). Propagation of Talaromyces flavus by a fermenter for greenhouse application to control sugar beet seedling damping-off. Acta Biologica Indica, 8(1):68-75.
_ Naraghi L. & Negahban M. & Heydari A. & Razavi M. & Afshari-Azad H. (2018a). Growth Inhibition of Fusarium oxysporum f. sp. lycopercisi, the Causal Agent of Tomato Fusarium Wilt Disease by Nanoformulations Containing Talaromyces flavus. Ekoloji, 106(1):103-112.
_ Naraghi L. & Negahban M. & Heydari A. & Razavi M. & Afshari-Azad H. (2018b). The effects of nanoparticles on sporulation and active population of Talaromyces flavus. International Journal of Bio-Technology and Research (IJBTR), 8(2):27-38.
_ Naraghi L. & Razi Nattaj M. (2022). Efficacy of Talaromyces flavus microcapsule in controlling cotton important fungal diseases. Revista De Gestao Social E Ambiental, 16(2):e03023.
_ Naraghi L. & Shahriari D. & Sarpeleh A. & Heydari A. & Afshari Azad H. (2017). Decrease in the incidence of cucumber Fusarium wilt in Varamin greenhouse using Talaromyces flavus. International Journal of Agricultural Scince and Research, 7(4):143-154.
_ Negahban M. & Moharramipour S. & Moharramipour M. & Zandi M. & Pezeshki MH. (2011). Oil nano-encapsulation by coacervation method on naturitional indices of Tribolium castaneum (Col: Tenebrionidae). The International journal of artificial organs, 34(8):667-667.
_ Nicolletti R. & Andolfi A. & Salvature MM. (2023). Endophytic fungi of the genus Talaromyces and plant health. Microbial Endophytes and Plant Growth, 1(1):183-213.
_ Ostertag F. & Weiss J. & McClements DJ. (2012). Low-energy formation of ediblenanoemulsions: factors influencing droplet size produced by emulsion phaseinversion. Journal of Colloid and Interface Science, 388(1):95-102.
_ Pereira I. & Ortegu R. & Barrientus L. & Moya M. & Reyes G. & Kramm V. (2009). Development of a biofertilizer based on filamentous nitrogen- fixing cyanobacteria for rice crops in Chile. Journal of Applied Phycology, 21(1):135-414.
_ Seefeldt SS. & Peters E. & Armstrong ML. & Rahman A. (2001). Cross-resistance in chlorsulfuron resistant chickweed (Stellaria media). New Zealand Plant Protection, 54(1):157-161.
_ Tian Y. & Zhao Y. & Fu X. & Yu C. & Gao K. & Liu H. (2021). Isolation and identification of Talaromyces spp. strain Q2 and its biocontrol mechanisms involved in the control of Fusarium wilt. Frontiers in microbiology, 12(1):2-14.
_ Wada R. & Fujimoto K. & Kato M. (2014). Why is poly (oxyethylene) soluble in water? Evidence from the thermodynamic profile of the conformational equilibria of 1, 2-dimethoxyethane and dimethoxymethane revealed by Raman spectroscopy. The Journal of Physical Chemistry B. 118(42):12223-12231.
_ Wang B. & Li L. & Lin Y. & Shen D. & Shao X. & Zhang C. & Qian G. (2022). Targeted isolation of biocontrol agents from plants through phytopathogen co-culture and pathogen enrichment. Phytopathology Research, 4(19):2-14.