Greenhouse and molecular evaluation of resistance of Iranian bean cultivars to Fusarium oxysporum f.sp. phaseoli, the causal agent of Fusarium wilt
Subject Areas : Plant Protection
Ehsan
Hasanvand
1
(Department of Plant Protection, Shiraz Branch, Islamic Azad University Shiraz, Fars, Iran)
seddiqe
mohammadi
2
(faculty member of Islamic Azad University, Shiraz Branch)
Keywords:
Abstract :
Fusarium wilt (yellow) that caused by the fungus Fusarium oxysporum f. sp. phaseoli, is an important disease and can cause severe damage to bean products world wide and cause reduced functional. Since farming techniques to control the disease is not completely effective, cultivars with genetic resistance to the disease are recommended. In order to identify resistant cultivars experiment in that randomized complete block design with three replications and 12 trkeatments, including different cultivars of beans. By method at a concentration suspension inoculation of root drench were inoculated. In root drench method suspension spores of pathogen because of healthy roots a lower percentage of spores could penetrating in roots. So it was far less severe symptoms and can say all cultivars has same statistical group and were resistant and infected cultivars tolerant. Considering the fact that the disease causes yellowing and wilting the factors concentration of chlorophyll a, chlorophyll b, and carotenoid concentrations and chlorophyll total concentrations were measured with evaluating resistant and tolerant cultivars were identified. Accordingly, cultivars Sayad and E9 respectively than other cultivars showed more resistant to the disease. Then the reaction is different bean cultivars to fungal F. oxysporum f.sp. phaseoli were evaluated using specific marker SCAR. According to cultivars Akhtar, Naz, Sadri, Sayad, E9 and WA with primer pairs SU20 have a band of 750 bp and A55 are resistant gene but cultivars Talash, Shekofa, Jegari, Aje, Khomeyn and capsules with specific primer pairs SU20 did not produce band.
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