List of Articles -Amylase

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  1 - Partitioning Studies of Alpha-amylase in Aqueous Two Phase System Composing of Polyethylene Glycol and Tri-Sodium Citrate
  مریم Karimi N. Hadidi Gh. Pazuki Sh. Abdolrahimi
  Introduction: One of the scenario for separation of biotechnology products is the application of aqueous two-phase systems (ATPS) rather than traditional extraction by using solvent. The ATPSs are applicable method for separation and purification of biomolecules mixture More

  Introduction: One of the scenario for separation of biotechnology products is the application of aqueous two-phase systems (ATPS) rather than traditional extraction by using solvent. The ATPSs are applicable method for separation and purification of biomolecules mixture. The ATPS can be applied for protein purification of biomolecules from suspension broth. The goal of the present research is the study of partitioning of -amylase in polymer-salt aqueous two-phase system composed of polyethylene glycol with molecular weight of 1000 and tri-sodium citrate salt as a biocompatible salt. Materials and Methods: The aim of this research is to study partitioning of α-amylase enzyme in an aqueous two phase system. The system consists of polyethylene glycol (PEG) with molecular weight of 1000 and tri-sodium citrate that is a biocompatible salt. Results: The results for partitioning of enzyme in ATPS has been modeled and statistically analyzed; and indicated that the partition coefficient of -amylase in the system has the maximum amount of 2.681. Conclusion: In this research it has been proved that ATPS components concentration has an effect on the enzyme partition coefficient. Using experimental results, -amylase has a great tendency to be placed in the PEG rich phase. Manuscript profile
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  2 - Evaluation of β-amylase Activity of Sweet Potato (Ipomoea batatas) Cultivated in Iran
  F. Hesam R. Taheri Tehrani G.R. Balali
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  3 - Isolation and Phylogenetic study of new amylase producing Bacillus strain from Ghainarjeh hot spring in East Azarbaijan
  Sahar Hosseini Mehdi Ebrahimi Maasoumeh Mahdavi Ourtakand Khadijeh Pourdadash Roozbeh Yalfani
  Background and objectives: Amylase is one of the important industrial enzymes, which is used in the food industry, detergent production, glucose, textile, and paper making. Bacillus spp. is considered as a major amylase producing microorganisms. The main objective of th More

  Background and objectives: Amylase is one of the important industrial enzymes, which is used in the food industry, detergent production, glucose, textile, and paper making. Bacillus spp. is considered as a major amylase producing microorganisms. The main objective of this study is isolation and phylogenic study of amylase producing thermophile Bacillus spp. from Ghainarjeh hot sprin in East Azarbaijan. Materials and methods: Samples from water of Ghainarjeh hot spring were cultured on recommended mediums. The grown colonies were tested for amylase production and positive colony determined a gram positive Bacillus using morphological and microscopically characteristics. Selected colony was used for total genomic DNA extraction, 16S rRNA fragment amplification and sequencing was done according to standard procedures. The sequence of 16S rRNA was used for phylogenetic investigation using maximum likelihood and maximum parsimony methods. Results: According to the results from morphological and biochemical investigations, the isolate is a gram positive, spore forming Bacillus with ability for use glucose, fructose and sucrose but not galactose and lactose. This isolate was positive for endol, citrate and VP but negative for catalase and oxidase. Conclusion: The results of phylogenetic investigation from both method show that the amylase producing isolated Bacillus is a new member of Bacillus Subtilis spp. in this study a new member of Bacillus subtilis spp. with the ability in amylase production was successfully isolated. Manuscript profile
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  4 - Phytochemical quantification, in vitro antioxidant and antidiabetic potentials of methanol and dichloromethane extracts of Triclisia subcordata (Oliv) leaves
  Olubunmi Adenike Akinwunmi Damilola Kayode Adekeye Sulaimon Adeoye Olagboye
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  5 - In-vitro alpha amylase and alpha glucosidase inhibitory potential of leaf hexane, ethyl acetate and methanol fractions of Pterocarpus soyauxii Taub
  Emmanuel Ojah Dorcas Olufunke Moronkola
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  6 - Immobilization of Bacillus alkalitelloris in agar for alpha-amylase enzyme production
  Marjan Alimohamadiyan Mehdi Ebrahimi Sahar Honarmand Jahromi
  Due to the high consumption of amylase, the use of amylase-producing strains and optimizing the production conditions of this enzyme is an efficient way to produce this enzyme. Due to the fact that the amylase gene of Bacillus alkalitelluris has not been identified so f More

  Due to the high consumption of amylase, the use of amylase-producing strains and optimizing the production conditions of this enzyme is an efficient way to produce this enzyme. Due to the fact that the amylase gene of Bacillus alkalitelluris has not been identified so far, it is important to achieve stable enzyme production conditions by the bacteria that produce these enzymes. One of the most important methods is to immobilization of the bacteria in suitable support so that the ability of these bacteria to produce enzymes is maintained. In this study, Bacillus alkalitelluris was first cultured in LB broth culture medium containing starch. The bacteria were then immobolized on agar and the amount of enzyme produced was measured using the DNS method. Finally, the amount of enzyme production when re-use stabilized cells in successive cultures was evaluated. According to the results, the bacterium Bacillus alkalitelluris immobilized in agar support is still able to produce alpha-amylase enzyme. Maximum alpha-amylase production was obtained after 24 hours. In addition, the immobilized bacteria remain after 4 times of changing the culture medium and are able to produce amylase enzyme so that up to 72 hours no significant change in enzyme production is observed. Due to the fact that the immobilized of Bacillus alkalitelluris in agar leads to the maintenance of alpha-amylase production capacity, so this method can be used in mass production of amylase enzyme. Manuscript profile
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  7 - Effect of Salicylic Acid and Ethephon on Seed Germination and Seedling Growth of Wheat under Salt Stress
  Soheyla Shakeri Fatemeh Saeid Nematpour Akbar Safipour Afshar
  Water or soil salinities are the most important factors that reduce the seed germination of plants. Ethephon can break seed dormancy in a variety of plants, such as cereals and speeds up germination. In some plants pretreatment of seeds with salicylic acid has increased More

  Water or soil salinities are the most important factors that reduce the seed germination of plants. Ethephon can break seed dormancy in a variety of plants, such as cereals and speeds up germination. In some plants pretreatment of seeds with salicylic acid has increased the germination percentage. To study effect of salicylic acid and ethephon on seed germination of wheat (Seivand cultivar) under salinity condition a factorial experiment in a completely randomized design with three replications was conducted at the Plant Research Laboratory of Neyshabur Branch of Islamic Azad University in 2011. Four salinity levels (0, 50, 100, 150 mM), three salicylic acid levels (0, 0.5, 1 mM) and four ethephon levels (0, 0.5, 1, 2 mM) were used. The results showed that at salinity condition seed germination rate and percentage, shoot and root length, their dry weight and α-amylase activity decreased and proline content increased. Pretreatment of seeds by salicylic acid increased seed germination percentage, some growth parameters, α-amylase activity and proline content under salinity condition. Moreover, pretreatment of seeds by ethephon decreased some growth parameters and increased proline content but its effect on germination and α-amylase activity were not significant. It seems that Salicylic acid as a plant growth regulator under salinity condition and ethephon convertion to ethylene, activated plant tolerance mechanisms to salinity condition and decrease damaging effect of salinity on seed germination and seedling growth of wheat. Manuscript profile
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  8 - مقایسه ترکیبات غذایی، خصوصیات HPLC، خاصیت آنتی اکسیدانی و ویژگی های نشاسته نان مرکب و نان گندم Sphenostylis stenocarpa
  صدیقات شدهنده ایندرانی داساپا پرابهاشانکار پیچان ساموئل اولوبود پرسوس آکینوسی
  10.30495/medherb.2022.698448
  Background & Aim: The use of composite flour and combined additives in wheat flour to improve their nutritional and health benefits have increased. This study focuses on the examination and comparison of the phenolic characterization, antioxidant properties, mineral More

  Background & Aim: The use of composite flour and combined additives in wheat flour to improve their nutritional and health benefits have increased. This study focuses on the examination and comparison of the phenolic characterization, antioxidant properties, mineral content, starch profile, in vitro starch digestibility and in vitro α-amylase inhibition present in produced composite bread and wheat bread.Experimental: Sphenostylis stenocarpa flour (SSF) and combined additives (dry gluten powder, fungal α-amylase and sodium stearoyl-2-lactylate) were incorporated into wheat flour to produce composite SSF bread. Wheat flour bread was prepared as a control.Results: The HPLC result showed higher values of gallic acid (1806.68 µg/100 g), p-coumaric acid (104.49 µg/100 g) and quercetin (22054.67 µg/100 g) in SSF bread while sinapic acid (195.88 µg/100 g), caffeic acid (1372.90 µg/100 g), ferulic acid (535.79 µg/100 g) were higher in control bread. Ferric-reducing antioxidant properties and mineral contents (Zinc, Ca, Fe, K, Mg, Mn and copper) were higher in SSF in comparison to control bread (P<0.05). The SSF bread had higher resistant starch and slowly digestible starch values but decreased total starch and rapidly digestible starch values. The in vitro starch digestibility (IVSD) value was also 0.54 times lower in SSF compared to control bread. The α-amylase inhibitory potential of SSF bread (56.77%) was significantly higher (P<0.05) in comparison to control bread (29.96%). It could be concluded that the incorporation of Sphenostylis stenocarpa in baked products such as bread will be of high nutritional benefits to humans.Recommended applications/industries: Sphenostylis stenocarpa is an underutilized bean that is rich in minerals, antioxidant properties and slow starch digestion potency which can be explored to prevent or manage the pathologic conditions that are related to sugar metabolisms. The utilization of underutilized Sphenostylis stenocarpa will go a long way in combating food insecurity. Manuscript profile
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  9 - خاصیت بازدارندگی آلفا-آمیلاز و آنتی اکسیدانی داروی گیاهی سیگناد گوگال در شرایط آزمایشگاهی
  اشوک کومار سالیمولا خان گوپی ستی ساران ناوین کومار لوکانت وی
  مقدمه و هدف: سیگناد گوگال یکی از داروهای گیاهی است که در طب آیورودا هند کاربرد فراوانی دارد. در تحقیق حاضر اثر عصاره آبی سیگناد گوگال بر بازدارندگی آلفا آمیلاز و خاصیت آنتی اکسیدانی آن مورد بررسی قرار گرفت.    روش تحقیق: به منظور بررسی اثر آنتی اکسیدانی ، از More

  مقدمه و هدف: سیگناد گوگال یکی از داروهای گیاهی است که در طب آیورودا هند کاربرد فراوانی دارد. در تحقیق حاضر اثر عصاره آبی سیگناد گوگال بر بازدارندگی آلفا آمیلاز و خاصیت آنتی اکسیدانی آن مورد بررسی قرار گرفت.    روش تحقیق: به منظور بررسی اثر آنتی اکسیدانی ، از روش قدرت احیا کنندگی FRAP و برای تعیین فعالیت بازدارندگی آلفا آمیلاز از روش دنیترو-سالیسیلیک اسید استفاده شد. نتایج و بحث: نتایج نشان داد که سیگناد گوگال دارای پتانسیل بازدارندگی آلفا-آمیلاز و آنتی اکسیدان به روش  FRAP با IC50 معادل 84 میکروگرم در میلی لیتر می باشد. توصیه کاربردی/ صنعتی: نتایج تحقیق حاضر بیانگر آن است که سیگناد گوگال ترکیبی از چند گیاه دارویی Commiphora mukul   Emblica officinalis, Terminalia belerica, Terminalia chebula, Ricinus communis قابلیت بسیار مناسبی آنتی اکسیدانی و بازدارندگی آلفا-آمیلاز را دارد. Manuscript profile
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  10 - Study of α-Amylase recovery by aqueous two-phase system in micro dimensions
  Farshad Raji َAhmad Rahbar-Kelishami
  10.30495/jacr.2022.692119
  The microfluidic aqueous two-phase system is a new method and is a suitable platform for the separation and recovery of biomaterials in the field of biotechnology. The combination of aqueous two-phase systems with microfluidic systems provides features that are not poss More

  The microfluidic aqueous two-phase system is a new method and is a suitable platform for the separation and recovery of biomaterials in the field of biotechnology. The combination of aqueous two-phase systems with microfluidic systems provides features that are not possible or difficult to achieve in macro methods. According to studies, aqueous two-phase system can be considered as a low-cost, environmentally friendly, and effective method for the separation of biomolecules, but its combination with microfluidic systems adds to its attractiveness. In the studied system, due to its dimensions, it showed the potential ability to accelerate the design and separation of biological processes. Alpha-amylase recovery was investigated with an aqueous two-phase polyethylene glycol/sodium citrate system on a glass microfluidic device made by the Co2-laser method. Effective parameters were tested with different values of concentration (125-150 mg / L) and flow rate (0.8-2 mL / h). The response surface method (RSM) was used to optimally determine the operational parameters. Transfer of alpha-amylase from the salt phase to the polyethylene glycol phase was performed with a parallel flow pattern. The values obtained at the optimal point also had a small error compared to the predicted value of the experimental design equations. The use of the microfluidic system studied in this study due to the micro dimensions will increase the recovery rate compared to macro systems, as well as the reduction of the time of this process in the micro dimensions compared to macro dimensions was significant. Manuscript profile
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  11 - Production and characterization of a thermophilic and extremely halotolerant alpha-amylase isolated from Anoxybacillus gonensis AT23
  Saeedeh Afrisham Arasto Badoei Delfard Abdolhamid Namaki Shoshtari Zahra Karami
  Background & Objectives: Thermophilic alpha-amylase can be used in different industries such as starch processing and detergents. This study was performed to isolate alpha-amylase-producing bacteria and characterization of the enzyme. Materials & Methods: After More

  Background & Objectives: Thermophilic alpha-amylase can be used in different industries such as starch processing and detergents. This study was performed to isolate alpha-amylase-producing bacteria and characterization of the enzyme. Materials & Methods: After sample collection from Gorooh hot spring in Kerman province, Iran, thermophilic alpha-amylase- producing bacteria were isolated using the starch-agar medium. 16S rDNA sequencing was used to identify the bacterial strain. Characterization of the thermophilic alpha-amylase was performed in the presence of various factors such as pH, temperature, metal ions, chemical compounds, and organic solvents. Also, kinetic parameters of the enzyme were determined in different concentrations of starch. Results: Anoxybacillus gonensis AT23 was identified as the best thermophilic alpha-amylase- producing strain. The alpha-amylase enzyme showed the optimal activity at pH 5 to 6. Sevenfold increase in the enzyme activity was observed in the presence of NaCl (3M). Mn2+ and Zn2+ increased the enzyme activity about 95% and 31%, respectively. Kinetic parameters including Km and Vmax were estimated about 1.657 mg/ml and 0.0059 mg/ml/min, respectively. Also, enzyme activity was also improved about 2 folds in the presence of organic solvents including n-butanol and 10% cyclohexane. Conclusion: Our results indicated that AT23 alpha-amylase is a halophile and organic solvent-tolerant enzyme. Therefore, it can be used in different industries. Manuscript profile
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  12 - Isolation of orange orchards rhizosphere Bacillus cereus by the ability of α-amylase secretion
  Mahnaz Ramezani Ali Riahi-Madvar Mooj Khaleghi
  Background and Objectives: Rhizosphere bacteria assist growth of plants via dissolving phosphate, production of special compounds such as phytohormones and release of hydrolytic enzymes such as a-amylase and kitinase. This study aimed to isolate and to identify a a-amyl More

  Background and Objectives: Rhizosphere bacteria assist growth of plants via dissolving phosphate, production of special compounds such as phytohormones and release of hydrolytic enzymes such as a-amylase and kitinase. This study aimed to isolate and to identify a a-amylase producing bacillus in rhizosphere of orange orchards and to investigate microbial activity to enzyme production in presence of different source of carbon ranges. Materials and Methods: This study was conducted in order to isolation of a-amylase bacilli from rhizosphere of orange orchards located at Kerman province, Iran. After sample collection, bacteria were isolated by growing on starch agar. Several molecular (based on 16SrRNA) and biochemical methods were used to identify bacterial species. The starch rapid hydrolysis test was used to investigate amylase activity of the isolates. Additionally, the enzyme production level was studied in the presence of several carbon sources include glucose, fructose and starch ranges. Results: Molecular and biochemical analysis showed that isolated bacterium is a strain of Bacillus cereus named as Bacillus cereus MR-R3 and recorded in GeneBank under accession number of KC306945.1. Moreover, results showed that starch and glucose have the highest positive effects on alpha amylase production in the presence of 0.5 g/l and fructose have the highest effect in the presence of 0.25 g/l. Conclusion: Isolation and identification of orange orchards rhizospher-derived bacillus species considering their ability to produce a-amylase and phosphate solvation showed that the presence of this species in this region is very important. Moreover, increase in production of the enzyme after treatment with different carbon sources can be related to their gene expression induction effects. Manuscript profile
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  13 - Identification and evaluation of antidiabetic activity of bacteria isolated from Persian Gulf sponges
  Atefeh Ansarizadeh Farshid Kafilzadeh Saeid Tamadoni Jahromi Mohammad Kargar Mohsen Gozari
  10.30495/jmw.2023.1977400.2048
  Background and purpose: Screening and identification of bacteria associated with sponges is an important step in the discovery of new drugs. The purpose of this research was to isolate and identify bacteria associated with sponges around Hormuz Island and to find bacter More

  Background and purpose: Screening and identification of bacteria associated with sponges is an important step in the discovery of new drugs. The purpose of this research was to isolate and identify bacteria associated with sponges around Hormuz Island and to find bacteria that produce metabolites that inhibit the activity of alpha-glucosidase and alpha-amylase enzymes. Materials and methods: In this study, 25 samples of Haliclona and Niphatea sponges were collected from 6 stations. Identification was done based on phenotypic characteristics. Bacteria were cultured in broth nutrient medium and their secondary metabolites were extracted by ethyl acetate. The inhibition rate of metabolites against alpha-amylase and alpha-glucosidase was evaluated based on colorimetric methods. The toxicity of metabolites against normal umbilical cord endothelial cell line was investigated. The productive bacteria were identified by polyphasic taxonomy approach. Results: A total of 105 bacteria were isolated. Vibrio and Bacillus bacteria with 32.81% and 17.19% in Haliclona sp. and 19.51% and 34.15% in Niphatea sp. The metabolites extracted from 3 isolates inhibited amylase enzyme activity with IC50 values ranging from 0.248 to 366.8 µg/ml. Also, 4 isolates produced inhibitory metabolites against alpha-glucosidase enzyme in IC50 values from 159.4 to 670.9 µg/ml. Based on the results of polyphasic identification of capable isolates including Bacillus pumilus HH 165, Pseudomonas lurida HH 124, Streptomyces sp. HN 235, Bacillus tequilensis HN 231. Conclusion: In this study, 3 strains of bacteria producing inhibitory compounds, including alpha-ambellase and alpha-glucosidase enzymes, and without cytotoxicity were identified. The mentioned bacteria can be suitable candidates in diabetes studies. Manuscript profile
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  14 - The effect of temperature and water absorption on enzymatic degradation of starch / polyvinyl alcohol blend film by α-Amylase
  Mohammad Taghi Taghizadeh Zahra Abbasi
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  15 - Momordica Foetida (Cucurbitaceae) Extract Alleviates Parastar (Insecticide) -Induced Toxicity on Pancreatic and Duodenal α-amylase Activity in Male Rats
  Edouard Nantia Akono Antoine Kada Sanda Faustin Pascal Manfo Tsague Aphrodite Choumessi Tchewonpi Tracy Suimula Kewir Stanley Tashi Carine Travert
  10.22034/jchr.2019.665820
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  16 - Inhibitory Effect of Phallusia Nigra (Savigny, 1816) Extract on Activity of Alpha –Amylase
  Asmae Tajik Mousa Keshavarz Ahmad Homaei
  10.22034/ascij.2022.1938794.1293
  Increased blood sugar levels play an important role in the development of diabetes. Thus, inhibition of alpha-amylase enzyme inhibits the conversion of polysaccharides to glucose or reduces it. In diabetics, this enzyme can be effective in absorbing glucose from the gas More

  Increased blood sugar levels play an important role in the development of diabetes. Thus, inhibition of alpha-amylase enzyme inhibits the conversion of polysaccharides to glucose or reduces it. In diabetics, this enzyme can be effective in absorbing glucose from the gastrointestinal tract and preventing a rapid rise in blood sugar. Ascidians are known as a group of marine fauna rich in bioactive secondary metabolites. This study was aimed at evaluating the inhibitory effect of alpha-amylase activity on Phallusia nigra. Specimens of P. nigra belonged to Qeshm and Hormoz islands. Under vacuum, using a rotary evaporator, the samples were extracted from polarity, i.e. ethyl acetate, methanol and water-methanol, respectively. Moreover, the percentage of alpha-amylase inhibitor activity was evaluated based on DNSA method in vitro and acarbose was used as a positive control. The results revealed that among all extracts, the highest inhibitory percentage was related to acarbose at a concentration of 2000 µg/ml equal to 69.65% and the lowest value was related to water-methanolic extract at a concentration of 500 µg/ml and equal to 15.39%. The highest inhibitory activity was observed as acarbose>ethyl acetate>methanol>water-methanol. Furthermore, the results showed a direct relationship between the level of enzyme inhibition and the concentration of extracts. In this study, ethyl acetate extract had the highest inhibitory effect of alpha-amylase enzyme with IC50 equal to 1327.244 µg/ml, followed by methanolic extract with IC50 equal to 1529.68 µg/ml. Water-methanolic extract had the lowest inhibitory effect with IC50 and equal to 2334.01 µg/ml. The inhibitory percentage of acarbose was higher than other extracts (IC50=1158.40 µg/ml). Due to the inhibitory power of P. nigra extracts, they can be used in the future in the production of anti-diabetic drugs with minimal or no adverse side effects. Manuscript profile
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  17 - Effect of pretreatment salicylic acid on germination and seedling growth in sweet corn (Zea mays L.) under salt stress conditions
  Prisa Sojoodi meysam oveysi Farshad Ghooshchi
  To study the effect of salicylic acid pretreatment on germination and alpha-amylase enzyme activity, sweet corn seedling in salinity, physiological testing Varamin Agricultural University - master design. Sweet corn seeds, varieties Saccharata after soaking in solution More

  To study the effect of salicylic acid pretreatment on germination and alpha-amylase enzyme activity, sweet corn seedling in salinity, physiological testing Varamin Agricultural University - master design. Sweet corn seeds, varieties Saccharata after soaking in solution (0, 0.5 and 1 mM) of salicylic acid for 24 h for germination under salt stress conditions, the paper roller dipped in a solution of NaCl concentrations (0, 25, 50, 100 and 200 mM) were transferred and in vitro tests. After germination, germination percentage, seedling dry weight and alpha-amylase enzyme activity was measured. Results showed that salinity reduces germination in seeds of sweet corn, and 200 mM salt concentration decreases, so 33.58 is the control of germination. Salicylic acid also was found to have a positive impact on germination as well as control and stress treatments increased germination will occur. Salicylic acid is used to increase seedling dry weight. Enzyme activity assays showed that the alpha-amylase enzyme activity in terms of reduced salinity and salicylic acid increases the activity of this enzyme, ie to reduce the effects of salinity. According to the obtained results it can be concluded that pretreatment of sweet corn seeds with salicylic acid, 1 mM Had a positive effect on germination and affects the antioxidant defense system of the plant sweet corn seedlings under salt stress is increased resistance. Manuscript profile