Effect of pretreatment salicylic acid on germination and seedling growth in sweet corn (Zea mays L.) under salt stress conditions
Subject Areas :Prisa Sojoodi 1 , meysam oveysi 2 , Farshad Ghooshchi 3
1 - Department of Plant Biology, Falavarjan Branch, Islamic Azad University, Falavarjan, Iran.
2 - Department of Agronomy, Faculty of Agriculture, Islamic Azad University Varamin - Pishva, Varamin, Tehran, Iran
3 - Assistant prof of Agronomy, Islamic Azad University Varamin Branch, Varamin-Pishva. Iran.
Keywords: germination, Salinity, Salicylic acid, sweet corn, enzymes alpha-amylase,
Abstract :
To study the effect of salicylic acid pretreatment on germination and alpha-amylase enzyme activity, sweet corn seedling in salinity, physiological testing Varamin Agricultural University - master design. Sweet corn seeds, varieties Saccharata after soaking in solution (0, 0.5 and 1 mM) of salicylic acid for 24 h for germination under salt stress conditions, the paper roller dipped in a solution of NaCl concentrations (0, 25, 50, 100 and 200 mM) were transferred and in vitro tests. After germination, germination percentage, seedling dry weight and alpha-amylase enzyme activity was measured. Results showed that salinity reduces germination in seeds of sweet corn, and 200 mM salt concentration decreases, so 33.58 is the control of germination. Salicylic acid also was found to have a positive impact on germination as well as control and stress treatments increased germination will occur. Salicylic acid is used to increase seedling dry weight. Enzyme activity assays showed that the alpha-amylase enzyme activity in terms of reduced salinity and salicylic acid increases the activity of this enzyme, ie to reduce the effects of salinity. According to the obtained results it can be concluded that pretreatment of sweet corn seeds with salicylic acid, 1 mM Had a positive effect on germination and affects the antioxidant defense system of the plant sweet corn seedlings under salt stress is increased resistance.
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