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Open Access Article
1 - The effect of endurance training with royal jelly consumption on dopamine in the hippocampus tissue of rats with Alzheimer's disease
Fakhradin Hassanlouei Seyed Ali Hoseini Laleh Behbudi Tabrizi Masod Haji Rasouli -
Open Access Article
2 - Effects of adding royal jelly to Tris-egg yolk extender on Afshari ram sperm parameters after liquid storage of semen
Amini, S., Masoumi, R., Rostami, B., Shahir, M.H. .The present study was conducted to evaluate the protective effects of royal jelly in Tris based extender on ram sperm parameters including motility, viability, morphology and plasma membrane integrity during 7 days liquid storage. 64 semen samples were collected with ar MoreThe present study was conducted to evaluate the protective effects of royal jelly in Tris based extender on ram sperm parameters including motility, viability, morphology and plasma membrane integrity during 7 days liquid storage. 64 semen samples were collected with artificial vagina from 4 rams. After initial assessments, semen samples were mixed and then were diluted with Tris based extender having 0 (control), %1, %3 and %5 royal jelly. Motility was assessed with CASA system. Morphology and viability were assessed with Eosin-Negrosin staining method and plasma membrane integrity was assessed with HOST method. Diluted semen samples were kept in 37° for 1 hour and 4° C for 7 days. The results showed that motility percentage (51.25±2.86) in %3 royal jelly supplemented group was significantly higher than other treatment groups after 7 days’ liquid storage of semen (P< 0.05). The percentage of sperm cells with functional plasma membrane (46.53± 2.96) in %3 royal jelly supplemented group was significantly higher than control group (P< 0.05). The percentage of live sperm cells in %3 (56.96±3.37) and in 1% (54.68±2.83) royal jelly supplemented groups were higher than control group (P< 0.05). In conclusion, the results of present study showed that supplementation of %3 royal jelly to Tris based extender improves ram sperm parameters after 7 days’ liquid storage of semen. Manuscript profile -
Open Access Article
3 - Protective effect of aerobic training and royal jelly on profibrogenic parameters of liver tissue and insulin resistance in obese rats
Ali Asghar Ma&#039;ghouli Ahmad Abdi asieh abbassi daloiiIntroduction: Obesity is characterized by several metabolic complications such as increased profibrogenic parameters of liver tissue. Most anti-obesity drugs have liver side effects. The aim of this study was to investigate the protective effect of aerobic training and MoreIntroduction: Obesity is characterized by several metabolic complications such as increased profibrogenic parameters of liver tissue. Most anti-obesity drugs have liver side effects. The aim of this study was to investigate the protective effect of aerobic training and royal jelly on profibrogenic parameters of liver tissue and insulin resistance in obese rats.Materials and Methods: In this experimental study, 45 male Wistar rats were divided into 5 groups: normal diet (ND), high fat diet (HFD), high fat diet - training (HFDT), high fat diet - royal jelly (HFDRJ) and high fat diet - training - royal jelly (HFDTRJ). The supplement groups received 100 mg of royal jelly (per kg of body weight) diluted in distilled water orally during the intervention period. Aerobic training program including running on treadmill with intensity of 50-60% VO2max was performed 5 days week for eight weeks. The genes expression of Transforming Growth Factor β (TGF-β) and Smad3 were measured by Real Time PCR. Results: There was significant increase of TGF-β, Smad3 and insulin resistance in HFD, HFDT, HFDRJ and HFDTRJ groups compared to ND group (P=0.001). Also, there was a significant decrease of TGF-β, Smad3 and insulin resistance in HFDT, HFDRJ and HFDTRJ groups compared to HFD group; and HFDTRJ compared to HFDRJ groups (P=0.001).Conclusion: It seems that the aerobic training with royal jelly can help to decrease of insulin resistance and improve the profibrogenic parameters of liver tissue in obesity caused by high-fat diet. Manuscript profile -
Open Access Article
4 - Identification of Bioactive Compounds from Brown Alga Cystoseria.Sp and Evaluation of Physicochemichal and Sensory Properties of Its Enriched Edible Jelly
M. Mohammadi GH. Mohebbi M. Bolourian A. Barmak E. EhsandoostIntroduction: Alga Cystoseria is a brown macroalga belonging to the Phaeophyta family. This alga with irregular branches is found in tropical, sub-tropical waters near rocks and wetlands. The aim of the study was to identify the bioactive compounds of Cystoseria brown a MoreIntroduction: Alga Cystoseria is a brown macroalga belonging to the Phaeophyta family. This alga with irregular branches is found in tropical, sub-tropical waters near rocks and wetlands. The aim of the study was to identify the bioactive compounds of Cystoseria brown algae extract and to evaluate the physicochemical and sensory properties of the edible jelly fortified with algae. Materials and Methods: The chemical compositions of the Cystoseria alga extract were detected by the GC-MS method, and the extracts were added to the jelly formulation at concentrations of 50, 100 and 150 mg, then the effects on sensory properties and synereses were studied. Sensory evaluations of oral jelly were carried out by ten evaluators and using a five-point hedonic test to examine the color, taste, texture and general acceptance of the prepared jelly. Results: The GC-MS analysis identified twelve biologically active compounds effective in food and drugs. The study indicated that the color score increased with the addition of algae extract. According to the results of acceptability, adding 50 mg of the extract to the jelly had the most effects on color, taste and texture factors. The syneresis reduction in treatments was an indication of high water content within the jelly, resulting in improving the appearance of edible jelly. Conclusion: It can be concluded that the addition of 50 mg of Cystoseria alga extract can be as criteria for the selection of the best formulation that can be used for its high nutritional value. Manuscript profile -
Open Access Article
5 - Preparation of Low Calorie Sour Tea Jelly Using Stevia and Gelatin of Chicken Skin- Evaluation of the Product
F. Yazdani Z. Raftani Amiri H. Shahiri TabarestaniIntroduction: The aim of this study was to produce low-calorie jelly using sour tea as a natural coloring agent, gelatin extracted from chicken skin with stevia as sweetener. Materials and Methods: Chicken skin gelatin was extracted by using alkaline-acid pre-treatment MoreIntroduction: The aim of this study was to produce low-calorie jelly using sour tea as a natural coloring agent, gelatin extracted from chicken skin with stevia as sweetener. Materials and Methods: Chicken skin gelatin was extracted by using alkaline-acid pre-treatment with 1 N of NaOH and 0.05 N hydrochloric acid. Gelatin was used at the concentrations of one and two percent (wt.%), Stevia with 0 to 100% replacement rates with sugar and sour tea at 20 wt.% for 100 g of jelly and 48 hours after gel production, pH measurement, Brix, moisture content, acidity, water activity, tissue stiffness, color, sensory analysis and microbial tests were performed. Experiments were conducted in a completely randomized design based on factorial experiment using SPSS software and compared with Duncan's multiple range test at 5% probability level. Results: The results showed that by increasing gelatin concentration and the level of sucrose replacement with stevia did not have a significant effect on pH. By increasing the concentration of gelatin, texture hardness and L*, significantly increased and moisture decreased (p < 0.05), whereas the parameters of brix, water activity, a* and b* of samples were not significantly different (p> 0.05). By increasing the levels of sucrose replacement with stevia and decrease of solids content the moisture, water activity and L *, a *, b * factors were significantly increased and the brix of the samples decreased. Conclusion: Sensory and physicochemical properties of sour jelly samples showed that using chicken skin gelatin and stevia as sweetener could be a good alternative to low-calorie jelly formulation by preserving physical, chemical and sensory properties. Due to medicinal properties, sour tea provides beneficial contribution to sour jelly product Manuscript profile -
Open Access Article
6 - Study on Predictive Models Relating Physico-chemical Properties of Iranian Royal Jelly and it’s Sensory Evaluation
soheila kamyab Maryam Gharachorloo Masoud Honarvar Mehrdad Ghavami -
Open Access Article
7 - Molecular Identification of Gelatin Origin in Pastilles and Jelly Products Collected from Tehran Markets
N. Kamandi M. Ghobadi Dana M. Ghavami -
Open Access Article
8 - Evaluation of the Activity of New Species of Jelly Fish Collected from Nayband Bay in Bushehr Against Human Pathogenic Bacteria
Akram Najafi Zahra Amini Khoei Saeid Tajbakhsh Golandam Asayesh Gholam hossein MohebbiInroduction & Objective:Currently, the presence of a wide range of bioactive compounds have been reported from the marine organisms worldwide. This study, for the first time, was aimed to evaluate the antimicrobial activity of water extraction of new species of jelly fi MoreInroduction & Objective:Currently, the presence of a wide range of bioactive compounds have been reported from the marine organisms worldwide. This study, for the first time, was aimed to evaluate the antimicrobial activity of water extraction of new species of jelly fish collected from Nayband Bay in Bushehr province. Material and Methods:In this study the jelly fish samples (Cassiopea andromeda) were collected from the Nayband Bay in Bushehr province. Minimum Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of the jelly fish extract were determined against five human pathogens. All the data were analyzed by SPSS software.Results: Our results showed that the jelly fish extract has both MIC and MBC effects against Escherichia coli and Pseudomonas aeruginosa bacteriaThis relation was statistically significant.Conclusion: According to our results jelly fish can be an appropriate marine source for antimicrobial components. Further in vivo investigations need to be carried out on its potential application in other aspects of medicine. Manuscript profile -
Open Access Article
9 - The Protective Effects of Royal Jelly on the Histomorphometric ,Hormonal Alternation and Oxidative Stress in Ofloxacin Treated Male Rat
Ghodrat Ebadi ManasInroduction & Objective: Royal jelly is a compound that is produced by the worker honey bees. This compound has antioxidant activity and protective effects against oxidative stress. The aim of this study were to determine protective role of royal jelly on decrease side MoreInroduction & Objective: Royal jelly is a compound that is produced by the worker honey bees. This compound has antioxidant activity and protective effects against oxidative stress. The aim of this study were to determine protective role of royal jelly on decrease side effects of ofloxacin and return reproductive function near normal rang in ofloxacin treated male ratMaterial and Methods:In this experimental work used 32 male albino rats that each has body weight between 160-18o gand accidentally divided to 4 groups: ofloxacin, royal jelly , ofloxacin with royal jelly and control. Each group included 8 rats. Dosage for ofloxacin group was 216 mg/kg.BW ofloxacin and for royal jelly group was 100 mg/kg.BW royal jelly. Royal Jelly with ofloxacin group received 216 mg/kg ofloxacin and100 mg/kg royal jelly. Control group received distilled water at dose of 216 mg/kg.BW. All groups received dose - related daily for 28 days orally. Data analyzed by one-way ANOVA and used Turkey HSD Post-test to compare the significance of difference. Results: After exposure ofloxacin results revealed thatdiameter of seminiferous tubules, thickness of epithelium seminiferous tubules, weight testes,FSH, LH, testosterone, TTM ,TAOCand RI in compared to control significance decline,but distance between seminiferous tubule ,TDI , SI, MDA and NO significanceincreased. All parameters were improved to near normal rang level after exposure royal jelly.Conclusion:This investigation revealed that ofloxacin has negative effect on histomorphometry and reproductive function in mature male rats,but at the same time if you use royal jelly it is protects against ofloxacin and retune to normal rang. Manuscript profile -
Open Access Article
10 - The Effects of Wharton's Jelly Mesenchymal Stem Cells Associated with Gum of Pistacia atlanticafor Burn Wound Healing
الهام حویزیBackground: Burn is a common wound in the world and consider the novel methods such as cell therapy can be a helpful strategy in the treatment. The purpose of the present study is investigating the effects of using ointment of animal oil mixed with Gum of Pistacia atlan MoreBackground: Burn is a common wound in the world and consider the novel methods such as cell therapy can be a helpful strategy in the treatment. The purpose of the present study is investigating the effects of using ointment of animal oil mixed with Gum of Pistacia atlantica associated wharton's jelly mesenchymal stem cells (WJMSCs) on rat third-degree burn models. Methods:In this experimental study, WJMSCs were extracted from human umbilical cord. For this study, 28 Wistar rats were burned by heating a metal rod of 1cm in diameter and then randomly divided into the control (7 rats) and treatment (21 rats) groups. The treatment group was divided into three groups (each group of 7 rats) of daily scrubbingof ointment, cell therapy, and cell therapy+ ointment. 106 cells (passage3) were injected into each rat subcutaneously. On day 30 after treatment, animals killed by chloroform and histological sections were prepared by staining Hematoxylsene-Eosin (H&E) and Trichromosone done for microscopic study. Results: Macroscopic and microscopic results indicated that in the experimental groups, the recovery was significantly more than the control. Also, the cell therapy+ ointment was significantly more effective than ointment and cell alone after 30 days. Histological analysis demonstrated a significant increase in angiogenesis, collagen synthesis, number of cells, thickness of skin layers, and totally acceleration wound healing in experimental groups compared to controls. Conclusion: Based on these data, it can be suggested that simultaneous cell-therapy and traditional medicine accelerate the repair of skin burns in the animal models more significantly. Manuscript profile -
Open Access Article
11 - A review of sugar substitutes: mannitol and maltitol
Mandana Zormand shila berenjiNowadays, an increasing trend is observed in the consumption of food that helps to improve health and reduce the risk of various diseases in consumers, in the meantime, a special type of carbohydrates called non-digestible carbohydrates (The major types of non-digestibl MoreNowadays, an increasing trend is observed in the consumption of food that helps to improve health and reduce the risk of various diseases in consumers, in the meantime, a special type of carbohydrates called non-digestible carbohydrates (The major types of non-digestible carbohydrates include non-starch polysaccharides (such as cellulose, pectin, and hemicellulose), resistant starch, and non-digestible oligosaccharides (such as fructo-oligosaccharide and xylo-oligosaccharide)) has attracted a lot of attention. The consumption of these compounds is currently increasing due to their beneficial physicochemical and physiological characteristics for the health of consumers. Carbohydrates are divided into monosaccharides, oligosaccharides or polysaccharides based on molecular weight or degree of polymerization (number of constituent monosaccharides). In general, sucrose substitute sweeteners can be divided into two categories: nutritious and non-nutritive sweeteners. Most sugar alcohols are considered nutritious sweeteners, including sorbitol (E420), mannitol (E421), isomaltose (E953), maltitol (E965), lactitol (E966) and xylitol (E967). The sweetness of sugar alcohols varies from 40-100% sucrose. Sugar alcohols are widely used in food products such as: jelly, chocolate and ice cream to bakery products. Recently, researchers have conducted several researches to investigate the production of jellies with different flavors and different formulations, including the production of low-calorie jellies. Substituting maltitol and mannitol instead of sugar had no significant effect on pH, acidity, dissolved solids (Degrees Brix) and moisture, and had a significant effect on syneresis, total sugar and tissue hardness. Manuscript profile -
Open Access Article
12 - The Investigation of Effective Factors in the Removal of Bentonite Jelly from the Joints of Cut-off Wall Panels
Seyed Majdeddin Mir Mohammad Hosseini Abdolhossein Azadmaneshb -
Open Access Article
13 - The effect of eight weeks of HIIT and N-chromosome royal jelly on liver enzymes and insulin resistance index in type 2 diabetic obese rats
sahar Riasati Hossein Abednatanzi Mandana GholamiBackground & Aim: Type 2 diabetes is the most common endocrine disease that occurs due to glucose intolerance due to imbalance between reserves and insulin demand, which leads to be changes in liver enzymes with structural and functional changes in the liver. The aim of MoreBackground & Aim: Type 2 diabetes is the most common endocrine disease that occurs due to glucose intolerance due to imbalance between reserves and insulin demand, which leads to be changes in liver enzymes with structural and functional changes in the liver. The aim of this study was to study changes in liver enzymes and insulin resistance index after HIIT and royal jelly in obese rats with type 2 diabetes. Materials & Methods: The statistical sample of the present study was 36 obese male rats with an average weight of 409 gr. After 20 weeks of high-fat diet, rats became diabetic by intraperitoneal injection of 25 mg/kg STZ body weight. Mice with fasting glucose between 150 and 400 mg /dl were considered to have type 2 diabetes. Diabetic rat in 4 groups of control (N=6), HIIT (N=8), royal gel (N=7), HIIT& Royal gel (N=8) group done and Accomplished Eight weeks of HIIT, 5d/w with intense 2-minute intervals of 80 to 90% VO2max and one-minute rest intervals with 50 to 56% VO2max. Royal jelly was given by gavage at the rate of 100 mg / kg 5 days a week. At the end of the protocol, blood samples were taken and liver enzymes and glucose were measured using an auto-analyzer and insulin was measured using a laboratory kit and analyzed using one-way and two-way analysis of variance and post hoc test. Results: showed that in comparison with the control group, HIIT led to a significant decrease in glucose and insulin resistance index and intermittent exercise resulted in a decrease in liver enzymes ALT, AST and ALP compared to the control group. The ALP decrease was significant (P = 0.001). Conclusion: It seems that HIIT and consumption of royal jelly can help reduce liver enzymes and improve liver status in rats. Manuscript profile -
Open Access Article
14 - The Effect of Aerobic Training with Royal Jelly Consumption on Insulin Resistance of Rats with MS
Abdolhamid Mosalla nezhad Farzaneh Taghian Khosro Jalali Dehkordi Seyed HosseiniIntroduction: Although the beneficial role of exercise and antioxidants in multiple sclerosis (MS) has been reported, the interactive effect of aerobic training (AT) and royal jelly (RJ) consumption on insulin resistance is still not well known. Therefore, the aim of th MoreIntroduction: Although the beneficial role of exercise and antioxidants in multiple sclerosis (MS) has been reported, the interactive effect of aerobic training (AT) and royal jelly (RJ) consumption on insulin resistance is still not well known. Therefore, the aim of the present study was to investigate the effect of of ET and RJ consumption on insulin resistance of MS rats.Materials and methods: In this experimental trial, 49 female Sprague-Dawley rats of EAE model weighing 180-210 gr were divided into seven groups based on their motor disability, including (1) experimental autoimmune encephalomyelitis (EAE), (2) sham (Sh), (3) 50 mg /kg of royal jelly consumption (RJ50), (4) 100 mg /kg of royal jelly consumption (RJ100), (5) aerobic training (AT), (6) AT+RJ50, and (7) AT+RJ100. In order to investigate the effects of EAE on the variables, 7 healthy rats were included in the healthy control group (HC). AT was performed for five weeks, four sessions per week at a speed of 11-15 m/min for 30 minutes, and RJ was injected intraperitoneally with the determined dose daily. To analyze the findings, one-way analysis of variance and Tukey's post hoc test were used (P≥0.05).Findings: Insulin resistance levels in the RJ50, RJ100, ET, ET+RJ50 and ET+RJ100 groups were significantly lower than the EAE group (P=0.01).Conclusion: It seems that aerobic training combined with royal jelly consumption has a synergistic and favorable effect on improving insulin resistance in rats with MS. Manuscript profile -
Open Access Article
15 - The effecte of eight weeks of resistance training with royal jelly on the pathaphysiological changes in the hippocampal tissue of Alzheimer's rats
Leila Mokhtari Tahereh Bagherpour Nematollah NematiAbstract Introduction: Oxidative stress plays role in the progression of Alzheimer's disease (AD).exercise and antioxidants on neuronal health have effect . the effect of eight weeks of resistance training (RT) along with royal jelly (RJ) on Malondialdehyde (MDA) MoreAbstract Introduction: Oxidative stress plays role in the progression of Alzheimer's disease (AD).exercise and antioxidants on neuronal health have effect . the effect of eight weeks of resistance training (RT) along with royal jelly (RJ) on Malondialdehyde (MDA), Protein Carbonylate (PC) and the pathological changes in the hippocampal tissue of Alzheimer's rats treated with trimethyltin (TMT)has been determined. Methods: In this experimental study, 42 male Sprague-Dawley rats treated with 8 mg/kg TMT were divided into TMT, Sham, RJ100, RJ200, RT, RT+RJ100 and RT+RJ200 groups. Supplemental groups received RJ with doses of 100 and 200 mg/kg/day as peritoneal injection, and the increasing RT protocol was performed for 8 weeks, 3 sessions a week with an intensity of 30 to 100% of the weight. Results: MDA, PC in TMT group were significantly higher than HC group. But MDA and PC in RT+RJ200 group were lower than TMT group (P≤0.05). Also, PC in RT group was significantly lower than TMT. MDA in RJ200 group were lower than TMT; PC values in RJ100 group was significantly lower than TMT (P≤0.05). The reducing effect of PC in RJ100 group was more favorable than RJ200 (P≤0.05). Also, the effect of reducing MDA in the RT+RJ200 group was more favorable than the RT+RJ100 group (P≤0.05). Conclusion:the resistance training and royal jelly have favorable effects on reducing oxidative stress. the simultaneous effect of these two interventions, especially with a higher dose, has a more favorable effect on reducing oxidative stress in the hippocampus tissue in AD modeling conditions. Manuscript profile -
Open Access Article
16 - The comparative study of antibacterial effects of Royal Jelly and Honey and the combination of them against standard strain of Helicobacter pylori
Nahid Rahimifard Behrang Hoseinzadeh shahram shoeibiHelicobacter pylori is a Gram-negative, microaerophilic spiral rod. About half of the world population is infected with the bacteria that cause gastric and duodenal ulcers, and if untreated and eradication lead to the gastric cancer. Treatment is not easy and requires t MoreHelicobacter pylori is a Gram-negative, microaerophilic spiral rod. About half of the world population is infected with the bacteria that cause gastric and duodenal ulcers, and if untreated and eradication lead to the gastric cancer. Treatment is not easy and requires the administration of high doses of combination antibiotic with extensive and repeated use, in addition to side effects, the incidence of bacterial resistance as a double challenge is posed. So finding an alternative treatment, such as new natural antimicrobial resources including bee products such as honey and royal jelly is necessary. Honey due to high osmolality, low acidity, and content of hydrogen peroxide and non-peroxide components and royal jelly because of Royalisin protein, fatty acid Trans-10-Hydroxy-2- Decenoic acid (10-HAD) and Jelleins peptides; they have a wide spectrum of antibacterial activity. In this study, anti-Helicobacter pylori property of thyme honey and Royal Jelly, harvested from hive located in Damavand region near Tehran-Iran, and mixture of them, against the standard strain of H.pylori ATCC 43504, was evaluated by Minimum Inhibitory Concentration (MIC) method. This method as the golden standard technique for determining the sensitivity of microorganisms to antimicrobial agents has been known and in accordance with standard procedures in the British Society For Antimicrobial Chemotherapy (BSAC) guidelines, was performed. Thyme honey and Royal Jelly and their mixture with positive control sample as cephalexin, had respectively 31.25, 62.5, 31.25, 0.0652 mg/ml MIC against standard strain of H. pylori. There was no synergy effect between honey and Royal Jelly in this study. Manuscript profile -
Open Access Article
17 - Feasibility study of producing and evaluation the quality properties of probiotic and synbiotic jelly containing Bacillus coagulans
Elham Rajab Pour Nikoo Samar Mansouripour Javad HamediIn this research, jelly samples including probiotic (containing Bacillus coagulans), synbiotic (containing B.coagulans and galactooligosaccharide) and control (without probiotic and prebiotic) were produced and kept in the refrigerator for six weeks. Survival of probiot MoreIn this research, jelly samples including probiotic (containing Bacillus coagulans), synbiotic (containing B.coagulans and galactooligosaccharide) and control (without probiotic and prebiotic) were produced and kept in the refrigerator for six weeks. Survival of probiotics was evaluated weekly. In order to confirm the safety, microbial tests including E.coli, Salmonella, S.aureus and lactic acid bacteria were carried out. Physicochemical properties of jelly samples including pH, brix and syneresis were considered for six weeks. Sensory evaluation was performed by taste panel assessment. The results showed that there was a decreasing trend of probiotics in probiotic and synbiotic jelly samples within six weeks. The samples kept their probiotic property until sixth week but it was more desirable almost until the fourth and fifth weeks. In safety microbial tests, no growth of the tested microorganisms was observed. Brix test results showed that the trend of brix reduction in probiotic and synbiotic samples was more evident than control from fifth week. This difference was also significant in the sixth week (p<0.05). The pH of the samples did not show significant difference before the fourth week (p>0.05). But after that, the pH of probiotic and synbiotic samples was significantly less than the control (p<0.05). According to the syneresis test, no syneresis was observed in the samples. The results of taste­­ panel showed no significant differences compared to control by adding probiotic or prebiotic to jelly samples (p<0.05). Therefore, according to the results of this research, probiotic and synbiotic jellies can be produced with proper quality characteristics. Manuscript profile -
Open Access Article
18 - Effect of in ovo Injection of Royal Jelly on Post-Hatch Growth Performance and Immune Response in Broiler Chickens Challenged with Newcastle Disease Virus
Y. Jafari Ahangari S.R. Hashemi A. Akhlaghi H. Atashi Z. Esmaili M. Ghorbani R. Mastani A. Azadegan H. Davoodi -
Open Access Article
19 - اثر تزریق داخل تخم مرغ ژل رویال بر جوجه درآوری، کیفیت جوجههای یکروزه، عملکرد و بعضی ویژگیهای ایمنی در جوجههای تازه تفریخ شده
م. گوهری ح.ر. خدائی م. طغیانیبه منظور بررسی اثر تزرق داخل تخم مرغ سطوح متفاوت ژل رویال بر میزان جوجه درآوری و کیفیت جوجههای یکروزه (راس 308) و بعضی ویژگیهای سیستم ایمنی آنها آزمایشی انجام شد. 640 تخم در قالب طرح کاملاً تصادفی به چهار سطح تزریق ژل رویال شامل: 1) یک میلیلیتر سرم فیزیولوژی (کنترل)، Moreبه منظور بررسی اثر تزرق داخل تخم مرغ سطوح متفاوت ژل رویال بر میزان جوجه درآوری و کیفیت جوجههای یکروزه (راس 308) و بعضی ویژگیهای سیستم ایمنی آنها آزمایشی انجام شد. 640 تخم در قالب طرح کاملاً تصادفی به چهار سطح تزریق ژل رویال شامل: 1) یک میلیلیتر سرم فیزیولوژی (کنترل)، 2) 5/0 میلیگرم ژل رویال، 3) 75/0 میلیگرم ژل رویال، 4) یک میلیگرم ژل رویال اختصاص داده شدند. برای هر تیمار، چهار تکرار در نظر گرفته شد و 40 تخم در هر تکرار استفاده شد. در روز 14 انکوباسیون، محلول آزمایشی با استفاده از سرنگ انسولین با سوزنی به طول 2 سانتیمتر داخل محلول آلانتوئیک تزریق شد. بعد از 510 ساعت انکوباسیون، همه جوجههای هچ شده به خوب، متوسط و ضعیف دستهبندی شدند. شاخصهای مورد مطالعه در طول دوره پرورش شامل افزایش وزن، مصرف خوراک، ضریب تبدیل غذایی و ویژگیهای سیستم ایمنی بود. تزریق ژل رویال تفاوتی در سرعت تفریخ ایجاد نکرد، اما کیفیت جوجههای یکروزه را به طور معنیداری افزایش داد (05/0>P). علاوه بر این، افزایش وزن، مصرف خوراک و ضریب تبدیل غذایی در مقایسه با گروه کنترل به طور معنیداری بهبود یافت. تیتر آنتیبادی تحت تأثیر تیمارهای آزمایشی قرار نگرفت، اما در نتیجه تزریق ژل رویال افزایش یافت. براساس عملکرد جوجهها در دوره پرورش، میتوان نتیجه گرفت که بهترین عملکرد و پاسخ ایمنی در جوجههای تفریخ شده با تزریق 5/0 میلیلیتر ژل رویال به دست آمد. Manuscript profile -
Open Access Article
20 - The Comparison of Antioxidant Property of Enriched Jelly Gum with Chlorella vulgaris and Normal Jelly Gum
F. Arabsorkhi S. Safaeian L. Salimi Antioxidant is a substance which inhibits production of free radicals, autooxidation in foods and cancers. This study focuses on the antioxidant property and polyphenol compounds of enriched jelly gums enriched with green microalgae Chlorella vulgaris. Moisture, More Antioxidant is a substance which inhibits production of free radicals, autooxidation in foods and cancers. This study focuses on the antioxidant property and polyphenol compounds of enriched jelly gums enriched with green microalgae Chlorella vulgaris. Moisture, ash and acidity of samples were also evaluated. Jelly gums were produced with different amounts of dried powder of green microalgae Chlorella vulgaris. Antioxidant property of microalgae and products were evaluated by DPPH and polyphenol compounds were evaluated by folin-ciocalteu assay. Moisture, ash and acidity were compared with standards for jelly gums. According to the results, jelly gums hadsignificant antioxidant properties because of Chlorella vulgaris which was27 ± 0.3 mg gallic acid g/dw. This microalga contains the acceptable level of polyphenol compounds and also about 2 ± 1.3 mg equivalent gallic acid g/dw. Moisture was stable in all samples and equal to 12.2. Ash was increased in each evaluation but acidity was decreased every time. Comparing the results of antioxidant properties of these samples with different groups of foods in other studies, it can be perceived that the samples are in the category of high antioxidant property. Using green Chlorella vulgaris assures the presence of polyphenol compounds in the products. Neither moisture, ash nor acidity were non-compliant with the standards. Manuscript profile -
Open Access Article
21 - The Study of the Viability of Human Wharton's Jelly Mesenchymal Stem Cells in Alginate Capsules
Zahra Poursafavi Saeed Abroun Saeid Kaviani Jebeli Nasim Hayati RoudbariStem cells with high proliferative capacity can be isolated from different tissues of the body. These cells originate from the fetal mesoderm and are found in tissues such as bone marrow, fat tissue, amniotic fluid, and Wharton's jelly. In this study, the survival of Wh MoreStem cells with high proliferative capacity can be isolated from different tissues of the body. These cells originate from the fetal mesoderm and are found in tissues such as bone marrow, fat tissue, amniotic fluid, and Wharton's jelly. In this study, the survival of Wharton's jelly human mesenchymal stem cells inside alginate capsules after 7, 14 and 21 days has been investigated. In this experimental study, 10 umbilical cord samples were obtained from pregnant mothers during caesarean section, and the vessels of the umbilical cord samples were isolated. Then it was cultured in DMEM-HG medium containing 10% FBS serum for 5 days. To show the stemness of these cells, CD73, CD34 and CD45 markers were evaluated by flow cytometry technique. After confirmation, the cells were encapsulated in alginate hydrogels. The viability of encapsulated cells was evaluated by trypan blue and MTT. The results showed that the capsules are spherical and have a uniform border and are homogeneously dispersed throughout the capsule. Wharton jelly encapsulation of mesenchymal stem cells did not change their morphology and viability. After 21 days, the survival of the encapsulated cells was maintained. Alginate as a three-dimensional biodegradable scaffold with suitable cell viability can be used as a suitable option for cell therapy and tissue engineering with the property of non-graft rejection. Manuscript profile -
Open Access Article
22 - Protective Effect of Royal Jelly on Methotrexate Infertility in Female Rats
Atikeh Amindoost Mona Farhadi Parvin Torabzadeh -
Open Access Article
23 - Protective Effect of Royal Jelly on Methotrexate Infertility in Female Mice
Hossein Bakhoda Nematollah Nemati Tahereh BagherpoorMethotrexate is a chemotherapy drug that is widely used in neoplastic disorders and ectopic pregnancy. In previous research, the toxicity of methotrexate has been reported on the genital tract. The properties of Royal jelly such as impotence improvement, and antioxidant MoreMethotrexate is a chemotherapy drug that is widely used in neoplastic disorders and ectopic pregnancy. In previous research, the toxicity of methotrexate has been reported on the genital tract. The properties of Royal jelly such as impotence improvement, and antioxidant and anticancer function have been reported. Therefore, in the present research, the effect of Royal jelly on ovarian cells in NMRI female mice treated with methotrexate was investigated. In this study, 70 female mice were divided into: negative control (without injection), positive control (received 0.5 mg/kg methotrexate), sham, and three experimental groups (T1, T2, and T3 received methotrexate plus 25, 50 and 100 mg/kg Royal jelly). Intraperitoneal injection was performed for 8 days. After histological processing and staining, the ovarian tissue, primary, secondary and graafian follicles, yellow body, as well the number of generation was investigated. Data were analyzed by Tukey test and one way ANOVA. Results showed there was a significant decrease in the number of generation in positive control, but the number of generation in experimental groups returned to the initial state. Regarding the duration of fertility, there was a significant decrease in the fertility rate in T1 and T3 compared to positive control group. The results of estrogen hormone showed a significant decrease in positive control group compared to negative control and increased significantly in T1 and T2. The number of primary, secondary, and graafian follicles in T 2 and T3 showed a significant increase (P<0.001). According to the present study, it was found that with further investigations, the appropriate dose of Royal jelly could act as a protective agent against the side effects of methotrexate and improve fertility. Manuscript profile -
Open Access Article
24 - The Effect of Eight Weeks of Endurance Training on Positive and Negative Gradients with Royal Jelly on Hippocampal Concentration of Carbonyl Protein and Pain Tolerance Threshold of Trimethylettin-treated Alzheimer’s Rats
Hamid Mousazadeh Nematollah Nemati Tahereh BagherpoorIncreased oxidative stress causes neuronal damage and incidence of chronic pains in patients with Alzheimer’s disease (AD). The aim of this study was to investigate the effect of eight weeks of endurance exercises in positive (PST) and negative (NST) slope with ro MoreIncreased oxidative stress causes neuronal damage and incidence of chronic pains in patients with Alzheimer’s disease (AD). The aim of this study was to investigate the effect of eight weeks of endurance exercises in positive (PST) and negative (NST) slope with royal jelly (RJ) on the hippocampal concentration of protein-carbonyl (PC) and pain tolerance threshold (PT) of Alzheimer’s rats with trimethyltin (TMT). In this experimental study, 49 Alzheimer’s rats with intraperitoneal injection of 8 mg/kg neurotoxin TMT were randomly divided into seven groups :(1) AD, (2) sham (normal saline) (Sh), (3) PST, (4) NST, (5) PST + RJ, (6) NST + RJ, and (7) RJ. To investigate the effect of Alzheimer’s disease on research variables, seven rats were included in the healthy control group (C). PST (with the speed of 16 m/min at positive slope (+15%)) and NST (with the speed of 16 m/min at negative slope (-15%)) interventions were carried out for eight weeks, five sessions per week and 60 minutes per session. RJ (dissolved in normal saline) was intraperitoneally consumed for eight weeks at 100 mg/kg /day. One-way ANOVA test along with Tukey post-hoc test were used in SPSS 22 software to investigate the intergroup difference (P ≤0.05). NST caused to decrease hippocampal level of PC (P = 0.001) and PT, as well as PST caused to decrease PT (P = 0.001). NST + RJ caused to decrease the hippocampal levels of PC (P = 0.001) and PT (P = 0.001) in rats with Alzheimer’s disease. Moreover, PT significantly reduced in the PST + RJ group (P = 0.001). PC hippocampal levels in the NST + RJ group were lower than in the PST + RJ group (P = 0.001). It seems that NST alone and with RJ have analgesic (anti-pain) and oxidative stress reduce effects. Although the interaction of PST and RJ had analgesic effects, the need of more studies on oxidative stress reducing effects is felt following PST and RJ consumption. Manuscript profile -
Open Access Article
25 - The Interactive Effect of Running on Different Slopes with the Use of Royal Jelly on the Concentration of Some Antioxidants in an Animal Model of Trimethyltin-treated Alzheimer’s
Hamid Mousazadeh Nematollah Nemati Tahereh BagherpoorAlthough the role of exercise and royal jelly in the health of Alzheimer’s patients has been reported, the interaction effect of these two variables on superoxide dismutase (SOD) and glutathione peroxidase (GPx) is still not well understood. Therefore, the present MoreAlthough the role of exercise and royal jelly in the health of Alzheimer’s patients has been reported, the interaction effect of these two variables on superoxide dismutase (SOD) and glutathione peroxidase (GPx) is still not well understood. Therefore, the present study was aimed at investigating the interactive effect of positive slope running (PSR) and negative slope running (NSR) with royal jelly on SOD and GPx concentrations in a trimethyltin (TMT) Alzheimer’s animal model. In this experimental trial, 49 rats in the age range of 8-10 weeks, weighing 190-220 g, Alzheimer’s with 8 mg/kg TMT were assigned into seven groups including group one: TMT, group two: sham, group three: Royal jelly, group four: positive slope, group five: negative slope, group six: royal jelly + positive slope and group seven: Royal jelly + negative slope. Moreover, to investigate the effect of Alzheimer’s induction on the research variables, seven healthy rats were included in the healthy control group following the positive slope and negative slope exercises for eight weeks, three sessions per week and each session ran for 60 minutes on a positive 15 and negative 15 slope on the treadmill. Besides, 100 mg/kg of royal jelly was peripherally injected into rats daily. One-way analysis of variance (ANOVA) with Tukey post hoc test and two-way ANOVA with Bonferroni post hoc test in SPSS 22 software were used to analyze the data (p ≤ 0.05). Negative slope increased SOD and GPx in hippocampal tissue of AD rats. PSR and royal jelly increased GPx. Positive slope and royal jelly and negative slope and royal jelly groups had a significant effect on increasing SOD and GPx. Positive and negative slopes and royal jelly seem to improve antioxidant defense; furthermore, the interaction of positive slope and royal jelly and the interaction of negative slope and royal jelly are more desirable than the effect of each alone. Manuscript profile
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