List of Articles Mohammad Reza Zolfaghari4

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  1 - Biosurfactant Production in Halotolerant Actinomycets sp. Isolated from Saline Lack Soil in Qom city
  soheil Aghaei فرزانه فخاریان محمدرضا ذوالفقاری محمد سلیمانی درجاق
  Biosurfactants are surface-active compounds synthesized by a wide variety of microorganisms. They are molecules that have both hydrophobic and hydrophilic domains and are capable of lowering the surface tension and the interfacial tension of the growth medium. They are More

  Biosurfactants are surface-active compounds synthesized by a wide variety of microorganisms. They are molecules that have both hydrophobic and hydrophilic domains and are capable of lowering the surface tension and the interfacial tension of the growth medium. They are nontoxic biomolecules that are biodegradable. Halotolerant actinomycetes can isolate from saline soil and lake with a high concentration of sodium chloride like qom saline lake. Bacteria need to adapt when are under extreme condition like salinity, If adaptation to the environment is due to metabolic changes, the production of new bioactive compounds as biosurfactants may be expected. In this research, 100 actinomycete species isolated from qom saline lake which 15 isolate among them were able to tolerate high salt concentrations, up to approximately 10% of NaCl, these 15 halotolerant actinomycetes were examined for biosurfactant production by common tests ( growth on the hydrophobic resources, hemolysis of erythrocytes, oil spreading test, surface tention measurement and etc.) . according to the results 8 actinomycete isolates were selected as biosurfactant producer. Results show that the species 9 has the best potential production.. Manuscript profile
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  2 - Isolation and identification of a tellurite resistance bacteria from industry's wastewater at Bioremediation and study optimal conditions increasing the Elimination and bioreduction of tellurite
  Mahboubeh Soleimani Sasani Mohammad Reza Zolfaghary Mohammad Soleimani
  The extensive use of tellurium oxyanions, such as Tellurite,in various industries such as textile, tanning and plating ,which are highly toxic to microorganisms and eukaryotes , has increased environmental pollution. Potential application of Tellurite oxyanions bioreduc More

  The extensive use of tellurium oxyanions, such as Tellurite,in various industries such as textile, tanning and plating ,which are highly toxic to microorganisms and eukaryotes , has increased environmental pollution. Potential application of Tellurite oxyanions bioreduction in resistant bacteria in surroundings can be a valuable tool in biotechnology for Biological removing of tellurite from contaminated areas by microorganisms.84 strains of resistant bacteria to tellurite was separated from industry's wastewater. Tellurite resistance activity was evaluated using the minimum inhibitory concentration (MIC)method, concentration 0/1 to26mM potassium tellurite at 34°C for 7 days by the agar dilution method and QWTmb9 was isolated which able to tolerate and reduction very high concentration of 22 mM. This tolerance to tellurite compared with the threshold concentration of other isolated bacteria is remarkable. In order to evaluate strain's Tellurite removal, colorimetric method using a spectrophotometer and DDTC reagent (sodium-diethyl‌dithiocarbamate‌tri-hydrate, A340 nm) was used.The effects of various factors on the optimal growth and tellurite removal were investigated conditions Tellurite concentration, pH, temperature, aeration rate and different concentrations of NaCl. Due to the direct connection to oxyanions resistance and resistance to antibiotics, Antibiogram test was conducted.QWTmb9 isolated from wastewater of blanket textile, which maximum removal rates in 24h is in 0.4 concentrations of Tellurite, temperature 35°C, pH7.5 , 100RPM aeration and 170mM NaCl concentration. This strain is resistant to antibiotics penicillin, ampicillin, rifampin, tetracycline, chloramphenicol, neomycin, kanamycin and erythromycin.Gram-negative strains QWTmb9 can be a good candidate for elimination of toxic tellurite in industrial biotechnology. Manuscript profile
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  3 - Comparison of antimicrobioal effect of essential oil of Vitex agnus – castus with common antibiotics in vitro.
  mohammad dakhili Hamideh Afarin محمدرضا ذوالفقاری
  Vitex agnus – castus (VAC) as a plant in herbal medicine has been since centuries ago. It is a phytoestrogenic herb native to the Middle East and southern Europe. It has clinical usage in so many countries. In this research, the antimicrobial effect of essential o More

  Vitex agnus – castus (VAC) as a plant in herbal medicine has been since centuries ago. It is a phytoestrogenic herb native to the Middle East and southern Europe. It has clinical usage in so many countries. In this research, the antimicrobial effect of essential oil of VAC was investigated on Staphyloccocus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and was compared with Ampicilin, Gentamicin, Nystatin.Essential Oil of VAC was extracted by steam distillation, Chemical constituents was analyzed by gas chromatography-mass spectrometry. for evaluation of antimicrobial effect Disk diffusion and Macrodilution methods were used.Essentical oil of VAC inhibited the growth of Staphylococcus aureus and Candid albicans at Concentrations 56/25 and 112/5 respectively. by Disk diffusion method The inhibitory zones were 10 mm and 11 mm respectively This essential oil inhibited the growth of Pseudomonas aeruginosa and Escherichia coli at concentration 450 . by Disk diffusion method They didn’t show inhibitory zone.The results of this research indicated that the essential oil of VAC has antimicrobial effect, in comparison with antibacterial effect of Ampicilin and Gentamicin has significant difference (P<0/001) antifungal effect of this essential oil in comparsion with Nystatin doesn’t have significant difference (P>0/05). The majer constituents of oil were: Terpinolene, Caryophyllene, α-pinene. Manuscript profile
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  4 - Using a phenotypic method to confirm the presence of the efflux pump associated with resistance to ciprofloxacin in Pseudomonas aeruginosa isolated from skin samples
  Ali Rahmati Mohammad Reza zolfaghary
  Introduction One of the most important causes of nosocomial infections in patients with skin infections is Pseudomonas aeruginosa. The occurrence of antibiotic resistance with various mechanisms is one of the problems in treating these patients. The main role in the de More

  Introduction One of the most important causes of nosocomial infections in patients with skin infections is Pseudomonas aeruginosa. The occurrence of antibiotic resistance with various mechanisms is one of the problems in treating these patients. The main role in the development of multiple resistance to antimicrobial drugs is played by the MexAB efflux pumps. The aim of this study was to evaluate the presence of the MexAB efflux pumps in the Pseudomonas aeruginosa isolated from skin samples. Materials and Methods: In this study 200 swab specimens from patients with skin infections were collected in Qom hospitals. Pseudomonas aeruginosa specimens were confirmed by biochemical tests. The drug susceptibility pattern was compared to ciprofloxacin by disk diffusion method and the phenotypic study of the effects of efflux pumps was done using cartwheel method . Results: In this study, 89 (45%) samples were infected with Pseudomonas aeruginosa, of which 19 cases (21%) were resistant to ciprofloxacin and more than 54% of the presence of the pump in the Pseudomonas aeruginosa more than 54% of the presence of the pump in the Pseudomonas aeruginosa. Conclusion: the presence of more than 54% of the presence of the pump in the Pseudomonas aeruginosa, is important in order to suggest a suitable therapeutic model for the infected patients. Keywords: Pseudomonas aeruginosa, skin infections, efflux pump Manuscript profile