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Open Access Article
1 - Evolution of bovine embryos cleavage in co-cultuer M.S.Cs deried from adipose tissues rat
Mehdi Ahmadifar علی محمدعینی Reihane Nateghi Nazila وحیدی ایریسفلیObjectiveMesenchyme Stem cells (MSCs) are one of the widely used sources in tissue engineering and efficacy of cultural environment. In this survey, rat stem cells are used as coculture. Owing to main obstacle of embryo culture in the lab medium, the survival of embryo MoreObjectiveMesenchyme Stem cells (MSCs) are one of the widely used sources in tissue engineering and efficacy of cultural environment. In this survey, rat stem cells are used as coculture. Owing to main obstacle of embryo culture in the lab medium, the survival of embryo in lab is low in comparison with that of normal conditions. The purpose of this research is the survey of fertilization and culture of bovine embryos in mesenchymal stem cells derived from adipose tissue (ADMCs).ApproachIsolated ADMCs were cultured on 4-well dishes. Three days after cell culture, the bovine immature oocytes were transferred on this mono layer system and the in vitro matured oocytes were fertilized by sperms in another MSCs free environment subsequently. Then in vitro fertilized embryos were co-cultured with the same mono layer of ADMCs for seven days. During oocyte and embryo co-culture, the rates of maturation and cleavage were assessed.AchievementsThis study reveals that co-culture of embryos with mesenchymal stem cells could increase the survival and cleavage of embryos significantly (P<0.05). The maturation rate of bovine co-cultured oocytes (%82) in comparison with that of control group (%78) has increased significantly. Also, the rate of blastocyst formation in co-cultured embryos (%33) was analytically comparable with that of control group (%26).ConclusionWhat bolsters substantially the quality and development of embryo is coculture systems. Manuscript profile -
Open Access Article
2 - Take a look at assisted reproductive technology using freezing
mina ramezani shokoh cheginiInfertility has long been considered one of the important medical issues which by the passage of time and advances of knowledge and technology, many therapeutic strategies have been acquired. In cases where there are no natural fertility methods, assisted reproductive t MoreInfertility has long been considered one of the important medical issues which by the passage of time and advances of knowledge and technology, many therapeutic strategies have been acquired. In cases where there are no natural fertility methods, assisted reproductive technologies are used. Researchers believe that the most common methods of fertility preservation, such as :Ovarian cortex, oocyte and embryo cryopreservation , depends on several parameters, including: the type of problem and treatment, the infertility cause and also the patient's age and marital status. Nowadays with the advances of medical science, infertile women or women with high risk of diseases such as cancer or who for various reasons postponed childbearing age can maintain their fertility using cryopreservation methods including egg, embryo and ovarian cortex freezing. Using these methods will be different based on the cause of infertility, infertile women conditions or specific diseases or cancer,. During the last years, vitrification techniques have been introduced as a clinical practice which constantly being reviewed, compared and changed. Manuscript profile -
Open Access Article
3 - چکیده های لاتین شماره 1 سال95
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Open Access Article
4 - شناسنامه علمی شماره 1سال95
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Open Access Article
5 - An overview of the most important mechanisms and systems of targeted drug delivery
سید محمدجواد حسینی زادهAbstractDrug delivery is one of the most important and most complex pharmaceutical branch that today due to use of other sciences is expanding dramatically. These improvements, especially in the field of drug delivery mechanisms and systems is very effective and useful. MoreAbstractDrug delivery is one of the most important and most complex pharmaceutical branch that today due to use of other sciences is expanding dramatically. These improvements, especially in the field of drug delivery mechanisms and systems is very effective and useful. In this paper, drug targeting mechanisms such as Physical targeting, by progressing on the application of magnetic fields, ultrasound and electric fields is discussed. Passive targeting by its application on treatment of tumor tissue is explained. Active targeting with considering of the its Specificities is explained. Also delivery systems for drug targeting like Nanoscale Carriers including nano-liposomes, lipid nanoparticles, solid lipid nanoparticles and dendrimers is explained. At the end Targeted Prodrugs and Cellular Carriers and their progress as two other examples of drug delivery systems explained. Keywords: Drug delivery, Drug targeting, Drug targeting mechanisms, Drug targeting systems, Physical targeting, Passive targeting, Active targeting, Nanoscale Carriers, Targeted Prodrugs, Cellular Carriers Manuscript profile -
Open Access Article
6 - Different methods using GnRHa on out of season reproductive efficiency in male goldfish (Carassius auratus, Linnaeus 1758)
احسان احمدی فر محمدرضا ایمانپور کوروش امینی وحید زادمجید طیبه عنایت غلامپورThe present research aims to evaluate effects of different methods of LHRHa implants and injections on some biological properties of semen including sperm density, spermatocrit percentage, pH and duration of sperm motility during spermiogenesis, sperm volume during 21 d MoreThe present research aims to evaluate effects of different methods of LHRHa implants and injections on some biological properties of semen including sperm density, spermatocrit percentage, pH and duration of sperm motility during spermiogenesis, sperm volume during 21 days after treatment and testosterone hormone changes in the days after implant and injection (Spermiogenesis, 7, 14 and 21) after the treatment in male goldfish broodstock in non-reproduction season. Fish were included in 4 treatments (injection of normal saline 0.7%, EVAc 20 mg / kg body weight LHRHa with 40 micrograms / kg body weight Metoclopramide, cholesterol pellets containing 20 micrograms / kg body weight LHRHa with 40 mg / kg body weight Metoclopramide and 100 mg / kg LHRH with 40 mg / kg body weight Metoclopramide. sperm did not differ significantly between treatments (P ≥0.05). highest spermatocrit percentage was found in treatments EVAc and LHRHa (P ≥0.05). EVAc implants significantly increased sperm volume compared with other treatments during the 21 days after treatment (P ≥0.05). Mobility period in different treatments of male fish broodstock was differed significantly (P ≥0.05), so that mobility period in treatment EVAc mobility was higher than other treatments. PH of different treatments with the control group (Physiology serum injection) showed significant difference so that the lowest rate was observed in the control group (P ≥0.05). Changes in hormone testosterone showed that its rate in Spermiogenesis in EVAc and LHRHa injection treatments showed significant difference compared to other treats in off-season reproduction of goldfish Manuscript profile -
Open Access Article
7 - Effects of the prebiotic Inuline on functional of reproduction, gonad development, fecundity and sex ratio in Danio rerio
مهدیه فدایی احسان احمدی فر طیبه عنایت غلامپورThe use of probiotics and prebiotics for increasing the growth and resistance against disease are well documented in aquaculture but work on the effects of probiotics and prebiotics on the reproductive performance of fish, especially on ornamental fish, are lacking. We MoreThe use of probiotics and prebiotics for increasing the growth and resistance against disease are well documented in aquaculture but work on the effects of probiotics and prebiotics on the reproductive performance of fish, especially on ornamental fish, are lacking. We conducted a study to determine the effects of probiotic on gonad development, reproductive performance of Danio rerio. In this study, a diet based on a control diet (T0) and three experimental diets consisted of three levels of inulin: 1 (T1), 2 (T2) and 3 (T3) and these treatments were done with three replicated. The juveniles were fed experimental diets for eight weeks (until adulthood: average weight: 1.67 gr, average length: 5.4 cm). In this experiment reproductive performance in terms of egg diameter, absolute fecundity, sex ratio, gonadosomatic index, number of larvae, survival of juveniles and gonad development were examined. The results showed that probiotic treatments, reproductive performance including egg diameter, survival larvae, absolute fecundity, Gonadosomatic Index, length and weight of larvae in treatment T2 and T3 were significantly higher than other treatments (P <0.05). Also the results showed that the prebiotic inulin does not have a significant impact on the sex ratio in zebrafish (P> 0.05). According to the histology of the gonads, the use of dietary inulin accelerates the process of gonad development in zebrafish. Manuscript profile -
Open Access Article
8 - Morphometric relationships genus Colutea L. (Fabaceae) in Iran
لیلا میرزایی مصطفی اسدی ایرج مهرگان طاهرنژاد ستاریA multivariate statistical analysis was performed on morphological characters of Colutea L. species in Iran. Totally, 20 qualitative and quantitative morphological characters of species were studied. Statistical analyses were done by SPSS ver. 18.0 and different methods MoreA multivariate statistical analysis was performed on morphological characters of Colutea L. species in Iran. Totally, 20 qualitative and quantitative morphological characters of species were studied. Statistical analyses were done by SPSS ver. 18.0 and different methods of multivariate statistical survey of cluster analysis (Ward) and ordination (PCA) was used. Using the results from morphological studies and Differential characteristics obtained in the principal component analysis, two components were introduced in the first component, shape and length of the seeds, traits and positive coefficient. Results of cluster analysis were confirmed by results principal components analysis.classification of cluster analysis was confirmed by principal component analysis and good variety of different traits to species based on similarities and differences have separated and morphological traits caaaaaaa aaaa aaa aaa aaa aaa a a a a a a a a a a a n identify and classify species of this genus is the systematic application. Manuscript profile -
Open Access Article
9 - In vitro flowering ornamental and medicinal plant Rosa damascena Mill. Rosa miniature variety
Rose belong to Rosa genus and Rosaceae family. The tissue culture technique is useful for scale up to short time (in all of seasons) and secondary metabolites production and proliferation medicinal plants. Optimization of rosa micropropagation has been done in MS medium MoreRose belong to Rosa genus and Rosaceae family. The tissue culture technique is useful for scale up to short time (in all of seasons) and secondary metabolites production and proliferation medicinal plants. Optimization of rosa micropropagation has been done in MS medium with IBA (0.1 mg/l) and BAP (2 mg/l) during two subcultures. The number of bloom and flower, percentage of flowering, bloom formation of time and flower annthesis of time have been researched from 5 different In vitro flowering treatments, they were MS medium culture with IBA (0.5, 1, 1.5 mg/l), BAP (0.02, 0.05, 0.1 mg/l) and GA3 (0.2, 0.5 mg/l) during two subcultures. In vitro flowering did not initiate in one and five treatments, %46.6, %40 and %33.3 were in 2 to 4 treatments, respectively. The bloom formation time was 53-57 days and flower formation time was 61-67 days in 2 to 4 treatments, respectively. The statistical analysis showed means had significantly in 2 and 4 treatments. Optimization of rosa miniature In vitro flowering has been done in MS medium culture with IBA (1 mg/l), BAP (0.02 mg/l) and GA3 (0.2 mg/l). The bloom formation time and anthesis flower were in 53 days and 63 days. Manuscript profile
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