Number 45 Volume 12

Open Access Article

1 - Comparison of bioactive ingredients and anatomical structure of Fumaria vaillantii Loise and F. asepala Boiss
Monir Mohseni Azra Ataei Azimi Babak Delnavaz Hashemloian

Objective: Persian sedge (Fumaria vaillantii) and sepalless sedge (F. asepala) are two annual herbaceous plants that grow separately or together in plant communities. The aim of this research was to compare the biochemical composition and anatomical structure of these t More

Objective: Persian sedge (Fumaria vaillantii) and sepalless sedge (F. asepala) are two annual herbaceous plants that grow separately or together in plant communities. The aim of this research was to compare the biochemical composition and anatomical structure of these two species in order to determine the proximity of their medicinal properties. Materials and methods: The studied plant species were collected from the central part of Saveh in May 2018. In order to compare the medicinal value, some characteristics of two Fumaria including effective substances and the anatomical structure of leaves, stems, roots and fruits were compared. The amount of lipid, protein, organic matter, sugar, phenol, alkaloid, types of chlorophyll, minerals and peroxidase activity were identified and measured by calorimetry, spectrophotometry and thin layer chromatography methods. Anatomical structure was made by preparing transversal sections, stained with methylene blue and Carmen Zaji and studied with a light microscope. Findings: The differences in the content of lipid, protein, chlorophyll a and stem minerals, root phenol, fruit alkaloid, sugar and chlorophyll b were significant in two species, but the differences in the composition of other organs were not significant. The activity of peroxidase and the number of phenolic compounds in the organs of the two species were significantly different. Fatty acids were almost similar in all organs except the stem of the leafless shoot. The anatomical structure of the two Fumaria was also completely similar. Conclusion: The findings of this research showed that Iranian Shatre and Bekasbarg are rich in effective substances and the anatomical structure is almost similar. This result shows that their medicinal value is the same. Manuscript profile

Open Access Article

2 - Valuation of the genetic diversity of the plant plant, Plantago major L., with ISSR molecular markers in Borujerd region
Narjes Azadbar Zahra sadat Mousavi Khansari Mohammad Mehdi Dehshiri Reza Yari

Objective: Borujerd region in Lorestan province is one of the best and less known natural habitats of plantago major L. The aim of the current study is to identify its natural habitats in Borujerd region and to evaluate genetic diversity in ecotypes with ISSR molecular More

Objective: Borujerd region in Lorestan province is one of the best and less known natural habitats of plantago major L. The aim of the current study is to identify its natural habitats in Borujerd region and to evaluate genetic diversity in ecotypes with ISSR molecular markers. Materials and methods: In this research, the genetic diversity of 23 samples from 6 populations of Plantago major L. was studied. The extracted DNA of all 23 samples was done by the kit method. 10 primers with international codes were used for PCR reaction. The presence or absence of the band was stored as a one/zero matrix in the SPSS program. Findings: Out of the total 1632 bands produced, 200 bands (alleles) were polymorphism, which was calculated as 24% of polymorphism. The length of the generated fragments varied between 300 and 1650 bp. Primer P8 produced the most common bands with 61 bands and primer P9 produced the least common bands with 7 bands in the populations. The highest number of amplified bands was 206 bands related to P8 primer and the lowest number of produced bands was 125 bands related to P5 primer. Conclusion: The results show that the ISSR genetic markers, especially the P8 primer, can be used in phylogenetic studies of leek plant. Despite its small area, Borujerd region is suitable for the growth of various ecotypes of the Plantago major L, and therefore the preservation and maintenance of this natural habitat and the propagation of different ecotypes should be considered. Manuscript profile

Open Access Article

3 - Awareness and attitude of the companions of mothers hospitalized in the post-partum department of Valiasr Hospital(Aj) in Qom city towards post-partum depression
Malihe Mehrmanesh

Objective: Postpartum depression may have irreparable consequences for the mother, child and her family. The awareness and attitude of family members plays a very important role in early diagnosis and treatment of the disease as quickly as possible. Therefore, this rese More

Objective: Postpartum depression may have irreparable consequences for the mother, child and her family. The awareness and attitude of family members plays a very important role in early diagnosis and treatment of the disease as quickly as possible. Therefore, this research was conducted with the aim of determining the level of awareness and attitude of the companions of mothers hospitalized in the post-partum department of Valiasr(Aj) Hospital, Qom.Materials and methods: This cross-sectional descriptive study was conducted on 100 companions of mothers hospitalized in the post-partum department of Valiasr Hospital(Aj) Qom, using available sampling method. The data collection tool was a three-part questionnaire including personal information, awareness and attitude measurement. The data were analyzed with the help of descriptive and inferential tests by SPSS version 16 software.Findings: The results showed that 70% of the studied people had moderate knowledge about postpartum depression. There is a statistically significant relationship between the level of awareness of postpartum depression with the variables of age, level of education, spouse's education, number of pregnancies, occupation and type of delivery. 85% of people had a negative attitude towards postpartum depression. The relationship between education level, spouse's education, number of pregnancies and occupation, with attitude was statistically significant. But the relationship between attitude with age and type of delivery was not statistically significant.Conclusion: Considering the importance of family members in supporting and diagnosing postpartum depression as quickly as possible, it is necessary to increase the awareness and attitude of those around pregnant mothers with different educational methods. Manuscript profile

Open Access Article

4 - Identification of soil actinomycetes and their effects in olive (Olea europea L.) variety Conservalia
Parvin Bagheri Farah Farahani soheil Aghaei

Objective: Actinomycetes are useful for biocontrol against soil fungi and increase seed growth, plant weight and yield in some crops. In this study, the isolation, identification and investigation of the population of Streptomyces species in the agricultural soil of C B More

Objective: Actinomycetes are useful for biocontrol against soil fungi and increase seed growth, plant weight and yield in some crops. In this study, the isolation, identification and investigation of the population of Streptomyces species in the agricultural soil of C Bagh Fadak area and the effect on the seed growth of olive (Olea europea L.) cultivar Conservalia were investigated. Materials and methods: In this study, actinomycetes were isolated from the soil of region C, then primary screening, culture and secondary screening were done by diffusion in agar and DNA extraction and the effect on seed growth of conservalia cultivar olives. DNA molecular identification was done for all the isolates andtheir  rRNA 16s genes were amplified by PCR reaction and the positive samples were sequenced. The results of phylogenetic analyzes of all recorded sequences of this gene were downloaded from the NCBI database and saved for phylogenetic analysis with Mega 7 software in FAST A format. The whole sequence of isolating, identifying and checking the population of Streptomyces species was entered into the software. Findings: 40 actinomycete bacteria were isolated from the soil of area C of Fadak garden. By performing specific tests, Streptomyces isolates were identified. Biochemical and morphological tests (stringy and white appearance) on 5 isolates confirmed the presence of streptomycetes. By producing auxin, the isolates were effective in increasing the rooting of Conservalia cultivar olive seeds. The rooting capacity of Streptomyces isolate D was statistically significant (probability level 0.05). Conclusion: Streptomyces of agricultural soils are effective on tree rooting.   Manuscript profile

Open Access Article

5 - Optimization of lipase enzyme activity and variables of fermentation conditions containing grape juice substrate in Aspergillus niger culture medium
Nima Zargaran Reza Habibipour Narges Ghobadi

Objective: Lipase enzyme is one of the important enzymes in food, detergent and textile industries. Also, today, industries related to this enzyme such as processing of organic materials, synthesis of biosurfactants, beverages and cosmetic, pharmaceutical and food indus More

Objective: Lipase enzyme is one of the important enzymes in food, detergent and textile industries. Also, today, industries related to this enzyme such as processing of organic materials, synthesis of biosurfactants, beverages and cosmetic, pharmaceutical and food industries have expanded significantly. In this regard, the aim of the current research is to optimize the activity of lipase enzyme and the variables of fermentation conditions containing grape juice substrate in Aspergillus niger culture medium. Materials and methods: In this research, for the first time, in order to achieve the maximum activity of the enzyme in the culture medium containing the carbon source of grape juice, the effective variables in the fermentation process of Aspergillus niger, i.e. temperature, acidity, concentration of grape juice and nitrogen source (yeast extract-peptone) with the help of Design Expert software was optimized. Findings: The acidity equal to 7.5, the amount of concentration of grape juice and the combination of yeast extract and peptone (with a ratio of two to one) were obtained with 1.5 and 0.75%, respectively. Lipase activity after optimizing the conditions of the culture medium was equal to 17.694 U/ml. Conclusion: Aspergillus niger records significant lipase activity in the culture medium with the factors of temperature, concentration of grape juice and yeast extract and peptone in its optimal amount and acidity equivalent to 7.5. Therefore, the carbon and nitrogen sources used in this research are suitable sources for use in the culture medium of this microorganism to produce lipase enzyme. Manuscript profile

Open Access Article

6 - The effect of different energy levels of diet on blood parameters and testicular tissue in sheep
Maryam Feli Saied Mohammadzadeh Mohsen Abasi

Purpose: One of the most important costs of sheep breeding is feed and the concentrated part of the ration. This research was conducted in order to investigate the effect of different rates of concentrate and fodder in lambs. Materials and methods: 27 Lori Bakhtiari ma More

Purpose: One of the most important costs of sheep breeding is feed and the concentrated part of the ration. This research was conducted in order to investigate the effect of different rates of concentrate and fodder in lambs. Materials and methods: 27 Lori Bakhtiari male lambs with an average age of 75 days and a weight of 26kg were selected and divided into three treatments and nine replications. Treatment one was 55:45 concentrate to fodder, second treatment was 70:30 and third treatment was 15:85 and feeding was done individually in morning and evening. After the end of the 100-day period, blood sampling was done and all the lambs were slaughtered. The testicles were removed from the scrotum and after measuring the morphometric parameters, the testicular tissue sample with dimensions of 1x1cm was prepared in 10% formalin solution and tissue sections were prepared. Findings: The results showed that the rate of concentrate and fodder made a significant difference in the macroscopic parameters of testicular tissue (P˂0.05). The weight of the testis in the treatment of three lambs decreased. In this treatment, the thickness of the coating of spermatogenic tubes, tube diameter, lumen diameter and tube area decreased. Also, the rate of hematocrit, red blood cells and hemoglobin of this treatment was significantly higher than other treatments (P˂0.05). Conclusion: increasing the percentage of concentrate more than 70% will have a negative effect. Manuscript profile

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