Identification of soil actinomycetes and their effects in olive (Olea europea L.) variety Conservalia

Subject Areas : microbiology

Parvin Bagheri ¹ , Farah Farahani ² , soheil Aghaei ³

1 - Masters, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
2 - Associate Professor, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
3 - Assistant Professor, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

Received: 2021-12-18 Accepted : 2022-06-10 Published : 2022-03-21

Keywords: Conservalia variety olive, Actinomycetes, Rhizobacteria, Streptomycete,

Abstract :

Objective: Actinomycetes are useful for biocontrol against soil fungi and increase seed growth, plant weight and yield in some crops. In this study, the isolation, identification and investigation of the population of Streptomyces species in the agricultural soil of C Bagh Fadak area and the effect on the seed growth of olive (Olea europea L.) cultivar Conservalia were investigated. Materials and methods: In this study, actinomycetes were isolated from the soil of region C, then primary screening, culture and secondary screening were done by diffusion in agar and DNA extraction and the effect on seed growth of conservalia cultivar olives. DNA molecular identification was done for all the isolates andtheir  rRNA 16s genes were amplified by PCR reaction and the positive samples were sequenced. The results of phylogenetic analyzes of all recorded sequences of this gene were downloaded from the NCBI database and saved for phylogenetic analysis with Mega 7 software in FAST A format. The whole sequence of isolating, identifying and checking the population of Streptomyces species was entered into the software. Findings: 40 actinomycete bacteria were isolated from the soil of area C of Fadak garden. By performing specific tests, Streptomyces isolates were identified. Biochemical and morphological tests (stringy and white appearance) on 5 isolates confirmed the presence of streptomycetes. By producing auxin, the isolates were effective in increasing the rooting of Conservalia cultivar olive seeds. The rooting capacity of Streptomyces isolate D was statistically significant (probability level 0.05). Conclusion: Streptomyces of agricultural soils are effective on tree rooting.  

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