شناسایی اکتینومیست‌های خاک و اثرات آن‌ها در زیتون (Olea europea L.) رقم کنسروالیا
محورهای موضوعی :
میکروب شناسی
پروین باقری
1
,
فرح فراهانی
2
,
سید سهیل آقائی
3
1 - کارشناسی ارشد، گروه میکروبیولوژی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران
2 - دانشیار، گروه میکروبیولوژی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران
3 - استادیار، گروه میکروبیولوژی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران
تاریخ دریافت : 1400/09/27
تاریخ پذیرش : 1401/03/20
تاریخ انتشار : 1401/01/01
کلید واژه:
استرپتومیست,
زیتون رقم کنسروالیا,
اکتینومیست‌ها,
ریزوباکترها,
چکیده مقاله :
هدف: اکتینومیست‌ها برای بیوکنترل علیه قارچ‌های خاک مفید و در برخی از گیاهان زراعی رشد دانه، وزن بوته و عملکرد را افزایش می‌دهند. در این مطالعه جداسازی، شناسایی و بررسی جمعیت گونه‌های استرپتومیست خاک کشاورزی منطقه Cباغ فدک و تاثیر بر رشد دانه رست‌های زیتون (Olea europea L.) رقم کنسروالیا مورد بررسی قرار گرفت.
مواد و روش‌ها: در این پژوهش جداسازی اکتینومیست‌ها از خاک منطقهC انجام شد، سپس غربالگری اولیه، کشت و غربالگری ثانویه با انتشار در آگار و استخراج DNA و تأثیر بر رشد دانه رست‌های زیتون رقم کنسروالیا انجام گرفت. شناسایی مولکولی DNA برای تمام جدایه‌ها انجام و ژن rRNA 16s آن‌ها با واکنش PCR تکثیر و نمونه‌های مثبت، سکانس شدند. نتایج حاصل از آنالیزهای فیلوژنتیکی تمام سکانس‌های ثبت شده این ژن، از بانک اطلاعاتی NCBI دانلود شدند و برای بررسی فیلوژنتیکی با نرم‌افزار Mega 7 با فرمت FAST A ذخیره گردید. تمام سکانس جداسازی، شناسایی و بررسی جمعیت گونه‌های استرپتومیست‌ها وارد نرم‌افزار گردید.
یافته‌ها: از خاک منطقهC باغ فدک 40 باکتری اکتینومیست جداسازی شدند. با انجام تست‌های اختصاصی، جدایه‌های استرپتومیست مورد شناسایی قرار گرفتند. تست‌های بیوشیمیایی و مورفولوژیکی (ظاهر رشته‌ای و سفید) بر روی 5 جدایه، وجود استرپتومیست‌ها را تایید نمودند. جدایه‌ها با تولید اکسین، در افزایش ریشه‌زایی دانه رست‌های زیتون رقم کنسروالیا موثر بودند. ظرفیت ریشه‌زایی جدایه D استرپتومیست از نظر آماری معنی‌دار بود (سطح احتمال 05/0).
نتیجه‌گیری: استرپتومیست‌های خاک‌های کشاورزی، بر ریشه‌زایی درختان موثر هستند.
چکیده انگلیسی:
Objective: Actinomycetes are useful for biocontrol against soil fungi and increase seed growth, plant weight and yield in some crops. In this study, the isolation, identification and investigation of the population of Streptomyces species in the agricultural soil of C Bagh Fadak area and the effect on the seed growth of olive (Olea europea L.) cultivar Conservalia were investigated.
Materials and methods: In this study, actinomycetes were isolated from the soil of region C, then primary screening, culture and secondary screening were done by diffusion in agar and DNA extraction and the effect on seed growth of conservalia cultivar olives. DNA molecular identification was done for all the isolates andtheir rRNA 16s genes were amplified by PCR reaction and the positive samples were sequenced. The results of phylogenetic analyzes of all recorded sequences of this gene were downloaded from the NCBI database and saved for phylogenetic analysis with Mega 7 software in FAST A format. The whole sequence of isolating, identifying and checking the population of Streptomyces species was entered into the software.
Findings: 40 actinomycete bacteria were isolated from the soil of area C of Fadak garden. By performing specific tests, Streptomyces isolates were identified. Biochemical and morphological tests (stringy and white appearance) on 5 isolates confirmed the presence of streptomycetes. By producing auxin, the isolates were effective in increasing the rooting of Conservalia cultivar olive seeds. The rooting capacity of Streptomyces isolate D was statistically significant (probability level 0.05).
Conclusion: Streptomyces of agricultural soils are effective on tree rooting.
منابع و مأخذ:
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Narayana KJP, Prabhakar P, Vijayalakshmi M, Venkateswarlu Y & Krishna PSJ. Study on bioactive compounds from Streptomyces sp. ANU 6277. Polish Journal of Microbiology. 2008; 57(1): 35-39.
Compant S, Clément C & Sessitsch A. Plant growth-promoting bacteria in the rhizo and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization. Soil Biology and Biochemistry. 2010; 42(5): 669–678.
DOI: https://doi.org/10.1016/j.soilbio.2009.11.024
Karimi I & Sadeghi A. The effect of silver nanoparticles on the bacterial flora of balk soil and cotton rhizosphere. Modern Genetics. 2018; 4(1): 27-33.
Zakir Bostan Abad S, Hashemi Shahraki AR, Haydaria P & Hosseinai M. Isolation and antimicrobial properties of actinomycetes isolated from the deserts of Iran. News of cellular-molecular biotechnology. 2013; 4(16): 4-15.
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Jabari M, Matroudi S, Zulqarnain H, Sharfi A & Zamani I. Isolation of Actinomyces tahai from the intertidal sediments of Dilem beach and study of their antifungal properties. Iranian Medical Microbiology. 2014; 9(4): 15-25.
Aghai SS, Fakharian F, Zulfiqari MR & Soleimani Darjag M. Production of biosurfactant in halotolerant actinomycetes isolated from the soil of Namak Qom lake. Applied Biology. 2014; 4(3): 1-11.
Golinska P & Dahm H. Occurrence of actinomycetes in forest soil. 2011; 66: 3-13.
Shivalee A, Mahesh D, Sandhya G, Sarfaraz A & Lingappa K. Isolation and screening of soil microbes for extracellular chitinase activity. Journal of Advanced Scientific Research. 2011; 7(2): 10-14.
Hotam S, Jayprakash Y, Smriti S & Natarajan G. Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India). Journal of Advanced Pharmaceutical Technology & Research. 2013; 4(2): 45-57.
Bharti A, Kumar V, Gusain O & Bisht GS. Antifungal Activity of Actinomycetes Isolated from Garhwal Region. Journal of Science Engglish and Technology. 2010; 2:3-9.
Deepthi MK, Sudhakar MS & Devamma MN. Isolation and Screening of Streptomyces Sp. From Coringa Mangrove Soils for Enzyme Production and Antimicrobial Activity. International Journal of Pharmaceutical, Chemical and Biological Sciences. 2012; 2(1): 110-116.
Pandey A, Ali, I, Butola KS, Chatterji T & Singh V. Isolation and characterization of actinomycetes from soil and evaluation of antibacterial activities of actinomycetes against pathogens. International Journal of Applied Biology and Pharmaceutical Technology. 2011; 2(4): 384-392.
Toumatia O, Compant S, Yekkour A, Goudjal Y, Sabaou N, Mathieu F, Sessitsch A & Zitouni A. Biocontrol and plant growth promoting properties of Streptomyces mutabilis strain IA1 isolated from a Saharan soil on wheat seedlings and visualization of its niches of colonization. South African Journal of Botany. 2016; 105: 234-239.
DOI: https://doi.org/10.1016/j.sajb.2016.03.020
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DOI: https://doi.org/10.1099/ijs.0.02624-0
Gowdar SB, Deepa H & Amaresh YS. A brief review on biocontrol potential and PGPR traits of Streptomyces sp. for the management of plant diseases. Journal of Pharmacology and Phytochemistry. 2018; 7(5): 3-7.
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Cho SH, Han JH, Chi NS & Seung BK. Phylogenetic diversity of acidophilic sporoactinobacteria isolated from various soils. Journal of Microbiology. 2006; 44(6): 600–606.
Leontopoulos S, Skenderidis P, Kalorizou H & Petrotos Bioactivity Potential of Polyphenolic Compounds in Human Health and their Effectiveness Against Various Food Borne and Plant Pathogens. A Review International Journal of Food and Biosystems Engineering. 2017; 7(1): 1-19.
Salla TD, Ramos dS, Astarita LV & Santarém, ER. Streptomyces rhizobacteria modulate the secondary metabolism of Eucalyptus plants. Plant Physiology and Biochemistry. 2014; 85: 14-20. DOI: https://doi.org/10.1016/j.plaphy.2014.10.008
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Usha R, Ananthaselvi P, Venil CK & Palaniswamy M. Antimicrobial and antiangiogenesis activity of Streptomyces parvulus KUAP106 from mangrove soil. European Journal of Biological Sciences. 2010; 2:77-83.
Vurukonda SS, Giovanardi D & Stefani E. Plant growth promoting and biocontrol activity of streptomyces spp. As endophytes. International Journal of Molecular Sciences. 2018; 19(4). DOI: https://doi.org/10.3390/ijms19040952
Chaudhary HS, Soni B, Shrivastava AR & Shrivastava S. Diversity and versatility of actinomycetes and its role in antibiotic production. Journal of Applied Pharmaceutical Science. 2013; 3(8). DOI: https://doi.org/10.7324/JAPS.2013.38.S14
Narayana KJP, Prabhakar P, Vijayalakshmi M, Venkateswarlu Y & Krishna PSJ. Study on bioactive compounds from Streptomyces sp. ANU 6277. Polish Journal of Microbiology. 2008; 57(1): 35-39.
Compant S, Clément C & Sessitsch A. Plant growth-promoting bacteria in the rhizo and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization. Soil Biology and Biochemistry. 2010; 42(5): 669–678.
DOI: https://doi.org/10.1016/j.soilbio.2009.11.024
Karimi I & Sadeghi A. The effect of silver nanoparticles on the bacterial flora of balk soil and cotton rhizosphere. Modern Genetics. 2018; 4(1): 27-33.
Zakir Bostan Abad S, Hashemi Shahraki AR, Haydaria P & Hosseinai M. Isolation and antimicrobial properties of actinomycetes isolated from the deserts of Iran. News of cellular-molecular biotechnology. 2013; 4(16): 4-15.
Yousefi Z, Aghaei SS, Marvotti A & Zulfiqari MR. Isolation, molecular identification and phylogenetic analysis of actinomycetes producing antimicrobial substances against pathogenic pathogens from saline agricultural soils of Qom city. Journal of Qom University of Medical Sciences. 2018; 13(3): 63-73.
Jabari M, Matroudi S, Zulqarnain H, Sharfi A & Zamani I. Isolation of Actinomyces tahai from the intertidal sediments of Dilem beach and study of their antifungal properties. Iranian Medical Microbiology. 2014; 9(4): 15-25.
Aghai SS, Fakharian F, Zulfiqari MR & Soleimani Darjag M. Production of biosurfactant in halotolerant actinomycetes isolated from the soil of Namak Qom lake. Applied Biology. 2014; 4(3): 1-11.
Golinska P & Dahm H. Occurrence of actinomycetes in forest soil. 2011; 66: 3-13.
Shivalee A, Mahesh D, Sandhya G, Sarfaraz A & Lingappa K. Isolation and screening of soil microbes for extracellular chitinase activity. Journal of Advanced Scientific Research. 2011; 7(2): 10-14.
Hotam S, Jayprakash Y, Smriti S & Natarajan G. Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India). Journal of Advanced Pharmaceutical Technology & Research. 2013; 4(2): 45-57.
Bharti A, Kumar V, Gusain O & Bisht GS. Antifungal Activity of Actinomycetes Isolated from Garhwal Region. Journal of Science Engglish and Technology. 2010; 2:3-9.
Deepthi MK, Sudhakar MS & Devamma MN. Isolation and Screening of Streptomyces Sp. From Coringa Mangrove Soils for Enzyme Production and Antimicrobial Activity. International Journal of Pharmaceutical, Chemical and Biological Sciences. 2012; 2(1): 110-116.
Pandey A, Ali, I, Butola KS, Chatterji T & Singh V. Isolation and characterization of actinomycetes from soil and evaluation of antibacterial activities of actinomycetes against pathogens. International Journal of Applied Biology and Pharmaceutical Technology. 2011; 2(4): 384-392.
Toumatia O, Compant S, Yekkour A, Goudjal Y, Sabaou N, Mathieu F, Sessitsch A & Zitouni A. Biocontrol and plant growth promoting properties of Streptomyces mutabilis strain IA1 isolated from a Saharan soil on wheat seedlings and visualization of its niches of colonization. South African Journal of Botany. 2016; 105: 234-239.
DOI: https://doi.org/10.1016/j.sajb.2016.03.020
Ghadeer S. Molecular studies on some soil- Streptomyces strains of western region, Kingdom of Saudi Arabia (KSA). African Journal of Biotechnology. 2013; 12(19):
2558–2564. DOI: https://doi.org/10.5897/AJB2013.11945.
Song J, Lee SC, Kang JW, Baek HJ & Suh JW. Phylogenetic analysis of Streptomyces spp. isolated from potato scab lesions in Korea on the basis of 16S rRNA gene and 16S–23S rDNA internally transcribed spacer sequences. International Journal of Systematic and Evolutionary Microbiology. 2004; 54(1): 203–209.
DOI: https://doi.org/10.1099/ijs.0.02624-0
Gowdar SB, Deepa H & Amaresh YS. A brief review on biocontrol potential and PGPR traits of Streptomyces sp. for the management of plant diseases. Journal of Pharmacology and Phytochemistry. 2018; 7(5): 3-7.
Drahansky M. We are IntechOpen, the world’s leading publisher of Open Access books Built by scientists, for scientists TOP 1 %. Intech, i(tourism). 2016; 13.
Cho SH, Han JH, Chi NS & Seung BK. Phylogenetic diversity of acidophilic sporoactinobacteria isolated from various soils. Journal of Microbiology. 2006; 44(6): 600–606.
Leontopoulos S, Skenderidis P, Kalorizou H & Petrotos Bioactivity Potential of Polyphenolic Compounds in Human Health and their Effectiveness Against Various Food Borne and Plant Pathogens. A Review International Journal of Food and Biosystems Engineering. 2017; 7(1): 1-19.
Salla TD, Ramos dS, Astarita LV & Santarém, ER. Streptomyces rhizobacteria modulate the secondary metabolism of Eucalyptus plants. Plant Physiology and Biochemistry. 2014; 85: 14-20. DOI: https://doi.org/10.1016/j.plaphy.2014.10.008
