Optimization of lipase Enzyme production in the native strain Halophile Haloarchaea Isolated from Iran southern soil

Subject Areas : microbiology

hossein vahidinejad ¹ ( دانشجو )
Ahmadali purbabaei ² ( هیئت علمی )
Mohammad hossein razavian ³ ( هیئت علمی )

Received: 2014-04-02 Accepted : 2014-05-28 Published : 2014-04-21

Keywords: Lipase, Haloarchaea, Optimization, Taguchi,

Abstract :

AbstractArchaea metabolites and enzymes widely used in various industries ,Because of the extremophiles nature and solidity And appropriate activities in the Hard industrial conditions. This study has been conducted According to the importance of lipolytic enzymes tolerance saline and alkaline conditions In the food, pharmaceutical and health Industry .Therefore Survey Influence Type and percent of substrate, percent of salt, pH, temperature and incubation time on the growth of Arkia strain Also Lipase Enzyme production was The aim of this study. Optimal conditions for strain growth and lipase enzyme production with a Taguchi method was concluded Respectively in the pH8 and 8, substrate 0.5 % & 1.5% ,time 6 and 7 day , salt 19% & 25% , temperature 45 ºC & 35 ºC in the presence of tween oil and sweet Almond oil as the sole carbon source.According to above results ,can use this strain in the tanning industry and extraction of oil.