Using a phenotypic method to confirm the presence of the efflux pump associated with resistance to ciprofloxacin in Pseudomonas aeruginosa isolated from skin samples
Subject Areas : microbiology
Ali
Rahmati
1
(Student)
Mohammad Reza
zolfaghary
2
(department of microbiology, faculity of sience, islamic azad university,qom, iran)
Keywords: skin infections, Pseudomonas aeruginosa, efflux pump,
Abstract :
Introduction One of the most important causes of nosocomial infections in patients with skin infections is Pseudomonas aeruginosa. The occurrence of antibiotic resistance with various mechanisms is one of the problems in treating these patients. The main role in the development of multiple resistance to antimicrobial drugs is played by the MexAB efflux pumps. The aim of this study was to evaluate the presence of the MexAB efflux pumps in the Pseudomonas aeruginosa isolated from skin samples. Materials and Methods: In this study 200 swab specimens from patients with skin infections were collected in Qom hospitals. Pseudomonas aeruginosa specimens were confirmed by biochemical tests. The drug susceptibility pattern was compared to ciprofloxacin by disk diffusion method and the phenotypic study of the effects of efflux pumps was done using cartwheel method . Results: In this study, 89 (45%) samples were infected with Pseudomonas aeruginosa, of which 19 cases (21%) were resistant to ciprofloxacin and more than 54% of the presence of the pump in the Pseudomonas aeruginosa more than 54% of the presence of the pump in the Pseudomonas aeruginosa. Conclusion: the presence of more than 54% of the presence of the pump in the Pseudomonas aeruginosa, is important in order to suggest a suitable therapeutic model for the infected patients. Keywords: Pseudomonas aeruginosa, skin infections, efflux pump
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