Number 23 Volume 6

Open Access Article

1 - Investigation of Soliton phenomena for laser transfer in optical fibers and its application in tissue treatment
zahra mahmoodzadeh zarandi parviz zobdeh

Abstract:Human skin has three-layer non-homogeneous tissue. The thermal and physical properties of each layer including Epidermis, dermis, and hypodermis are different from each other. In treatment procedures, laser source intensity is adjusted based on thermal amount w More

Abstract:Human skin has three-layer non-homogeneous tissue. The thermal and physical properties of each layer including Epidermis, dermis, and hypodermis are different from each other. In treatment procedures, laser source intensity is adjusted based on thermal amount which produced in each skin layer; furthermore, intensity control, optimization, and prediction of pulse shape before the interaction with tissue, is very important. Since, during the Laser-tissue irradiation, tissue temperature increases, and so uncontrollable temperature enhancement causes thermal harm on tissue.In this research, the Solitons general equation was considered. In order to having a predefined Gaussian shape of output laser pulse at the end of the transfer medium (optical fiber), numerical calculation was used. Solitons coefficients equation, include the dispersive coefficient, propagation constant, and coefficient of normalized loss has been obtained. The results could be used in determination of laser transfer medium structure, such as kind of material, coefficient of refractive, diameter and longitude length and etc. Recognition of fiber medium helps designers to provide a medium in order to establish Soliton, which input pulses are gained as output at the end of the fiber without any changes in shape. Access to different internal human organs could be possible by flexibility of optical fibers, as an important advantage. It seems, optimum parts determination of transfer medium may provide new horizon in controlled laser therapy. Manuscript profile

Open Access Article

2 - The study of gene expression of Bcl9 in patients with colorectal cancer
شهلا محمدگنجی بهروز طوفان ناهید مسعودیان

Background:Colorectal cancer(CRC)is the third most common cancer in men and the second most common cancer in women and the second leading cause of mortality among cancers in the world.Its incidence is increasing rapidly in Asia so about1.2 million people with CRC and mo More

Background:Colorectal cancer(CRC)is the third most common cancer in men and the second most common cancer in women and the second leading cause of mortality among cancers in the world.Its incidence is increasing rapidly in Asia so about1.2 million people with CRC and more than half of these patients die.In Iran during the past30years the incidence of CRC in men is more than and it is mostly in people over50years.The aim of this study is investigation of gene expression of BCL9 in patients in order to identify genes involved in CRC. M&M:In this study,the fresh tissue obtained from40patients with CRC after filling the consent form and questionnaire.The RNAextraction and cDNAsynthesis carried out.Then using specific primers for genes BCL9andB-actin,semi-quantitative RT-PCR experiments were performed.Findings:The results showed that the band of Bcl9 genes in all tumor samples while in normal samples or the band was not observed or a very weak band was visible.21men(52.5%)and19women(47.5%)with mean age of10 ± 56.1 years participated in this study, while77.5% of them (31out of 40) were over 50years of age.In point of TandN Factors,96.5% were patients withT2&T3(P=0.025) and 48.3% of patients showed lymph node invasion and22.5% showed metastasis (P=0.013).Conclusion: In this study BCL9 gene expression in tumor samples was increased 3.5-fold compared to normal samples. We suggest that BCL9 gene may act as one of the genes involved in colorectal cancer. Bcl9 interacts which with some genes, such as TCF, LEF, B-catenin and Pygo. Manuscript profile

Open Access Article

3 - شناسنامه علمی شماره 3 سال 95

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Open Access Article

4 - Prevalence of bacterial contamination of cord blood stem cells units stored in cord blood bank of the Academic Center for Education, Culture and Research (ACECR), Qom between 2012 to 2015
mohsen sheykhhasan maryam faraji mahdieh ghiasi

Background and Objectives: Umbilical cord blood has been used as a major source of hematopoietic stem cells to treat a variety of blood diseases. There is bacterial contamination risk during sampling from the umbilical cord and the processing related to it. The aim of t More

Background and Objectives: Umbilical cord blood has been used as a major source of hematopoietic stem cells to treat a variety of blood diseases. There is bacterial contamination risk during sampling from the umbilical cord and the processing related to it. The aim of this study was to determine the origin and type of bacterial contamination contaminated samples available in cord blood bank of the Academic Center for Education, Culture and Research (ACECR), Qom.Materials and Methods: In this study, 370 units of cord blood were collected and examined for bacterial contamination. In order to identify the source of pollution, preparation and determination culture strains was carry out in both blood sampling and finally to storage levels. Results were analyzed by SPSS software. Results: A total of 370 cord blood units, 5 units had bacterial contamination. The number of units in phase 2 was positive for bacterial cultures and 3 blood samples were also positive after processing.Conclusions: In this study, the rates of bacterial infections were reported as 5% in both the blood sampling and the processing, and the majority of isolated bacteria were of Staphylococcus aureus and Staphylococcus epidermidis, however, due to the high value and importance of blood stem cell cord and the risk of septic transplantation, develop training programs in order to obtain a sterile preparation is necessary. Manuscript profile

Open Access Article

5 - Detection of Methicillin-resistant Staphylococcus aureus Strains by Comparing Oxacillin and Cefoxitin Disk Diffusion methods and PCR for mecA Gene
reza yari Mohammad Reza Mehrabi Niloufar Gorbanpoor Kiasari

Detection of Methicillin-resistant Staphylococcus aureus Strains by Comparing Oxacillin and Cefoxitin Disk Diffusion and PCR for mecA Gene Abstract: Introduction and purpose: Methicillin-resistant Staphylococcus aureus, MRSA strains can pose a serious threat as nosocomi More

Detection of Methicillin-resistant Staphylococcus aureus Strains by Comparing Oxacillin and Cefoxitin Disk Diffusion and PCR for mecA Gene Abstract: Introduction and purpose: Methicillin-resistant Staphylococcus aureus, MRSA strains can pose a serious threat as nosocomial infections in various communities. In detection of these strains, PCR is an expensive method, whereas disk diffusion can provide a simple, inexpensive method with a high specificity. This study attempted to evaluate the disk diffusion method using cefoxitin and oxacillin discs compared with the PCR for detecting mecA gene. Materials and Methods: In this study, 150 Staphylococcus aureus isolates were collected from clinical skin samples from the patients and personnel in Qom. Next, methicillin resistance was diagnosed using oxacillin and cefoxitin disk diffusion method. Using PCR, mecA gene was identified in Staphylococcus aureus isolates and the data of both disk diffusion method and PCR were compared. Findings: Among the 150 isolates, 33% were methicillin-resistant in oxacillin disk diffusion, while 28% were methicillin-resistant in cefoxitin disc diffusion. The results revealed that oxacillin disk diffusion yielded false resistance to methicillin by 14% in detection of MRSA strains as compared to cefoxitin disk diffusion. Conclusions: In laboratories where molecular methods are not employed as a routine procedure for MRSA detection, cefoxitin disk diffusion can provide a simple, low-cost and promising alternative technique for detection of methicillin-resistant isolates. Keywords:MRSA, Cefoxitin, Oxacillin, mecA Manuscript profile

Open Access Article

6 - Evaluation of Antioxidant activity, Total phenolic and Flavonoid content of the extract of Adonis aestivalis L. collected from Lorestan Province
mahboubeh taherkhani مجید قربانی مرضیه حسینی

Adonis aestivalis L. belongs to the Ranunculoidae sub family of Ranunculaceae family. Up to now many reports has proven the presence of tetraterpenoids, phenolic compounds and cardiac glycoside in the genus Adonis. The most important mentioned effect of A. aestivalis is More

Adonis aestivalis L. belongs to the Ranunculoidae sub family of Ranunculaceae family. Up to now many reports has proven the presence of tetraterpenoids, phenolic compounds and cardiac glycoside in the genus Adonis. The most important mentioned effect of A. aestivalis is it's uses in the treatment of cardiac arrhythmia or sexual problems. The aim of this study was to evaluate the antioxidant activity, total phenolic and flavonoid content of the methanolic (%80) and ethyl acetate extract of A. aestivalis and compare them with each other. The total phenolic content (TPC) of the extracts of A. aestivalis was determined using the Folin ciocalteau assay at the presence of galic acid as standard and also the total flavonoid content (TFC) was determined using spectrophotometer methods at the presence of catechin as standard. Antioxidant activity of the extracts of A. aestivalis was determined upon Ferric-Reducing-Antioxidant power (FRAP) assay. The result showed that the total phenolic content of the methanolic extract was determined to be 607.262.35 mg GAE/g sample and total flavonoid content 97.810.007 mg catechin equivalent/g sample more than ethyl acetate extract (378.378.64 mg GAE/g sample, 89.730.003 mg catechin equivalent/g sample, respectively). Also the FRAP properties of the methanolic extract of A. aestivalis was determined to be 3.9850.001 mg GAE/g sample more than ethyl acetate extract (3.4940.0004) mg GAE/g sample. The results showed that the methanolic extract of A. aestivalis has high antioxidant activity, total phenolic and flavonoid content. Therefore further comprehensive investigation is required to determine the antioxidant properties of A. aestivalis. Manuscript profile

Open Access Article

7 - The comparison of Cichorium intybusL. hydroalcoholic extract and Nystatin on the isolated Candida albicans from vaginal infection under in vitro conditions
فاطمه ابراهیمی kambiz Roshanaii Fatemeh Jamallo

Background & Objective: With the increasing consumption of medical plants for medical treatment ,this topic of complementary medicine found a unique place. This study is the comparison of Cichorium intybusL. hydroalcoholic extract and Nystatin on the isolated Candid More

Background & Objective: With the increasing consumption of medical plants for medical treatment ,this topic of complementary medicine found a unique place. This study is the comparison of Cichorium intybusL. hydroalcoholic extract and Nystatin on the isolated Candida albicans from vaginal infection under in vitro conditions. Materials and Methods : C.Albicans was cultured on sabouraud dexterose agar containing chloramphenicol. Three series of 10 plant Cichorium intybusL. extract and a series of 10 of the disk impregnated with nystatin was prepared. On each plate, one plant extract disc, one nystatin disc (as positive control) and one blank disc (as negative controls) were placed. The mean diameter of non-growth halo around each plant extract was compared with the mean diameter of non-growth halo of positive control discs after 24 hours. Data was analysed by ANOVA statistical test (p<0.05). Results: The diameter of non-growth halo in nystation discs (28 mm) nystation were also more than Cichorium intybusL. discs(17 mm) (p<0.05). As well as the diameter of non-growth halo in Combination Cichorium intybusL and Nystatin discs(45 mm) was more than that for Cichorium intybusL discs(17 mm) and Nystatin discs(28 mm). Conclusion: Cichorium intybusL exhibited antifungal effects on C. Albincans But the effect of Nystatin on the lack of growth of Candida Albicans strains was greater than that in Cichorium intybusL. and combining the drug with extract on the lack of growth of Candida Albicans strains was greater than that in Nystatin. Key word: Antifungal effect, hydroalcoholic extract Cichorium intybusL. , Nystatin . Manuscript profile

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