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      • Open Access Article

        1 - Cryopreservation of Silybum marianum L. seeds.
        فریده صالح Shahin Mehrpoor fatemeh jamaloo
        Cryopreservation is one of the most appropriate and safest tool to preserve plant germplasm species including plants seeds. In this temperature, physiologic activities and metabolic processes of the seeds and plant members are almost held up and seeds are able to surviv More
        Cryopreservation is one of the most appropriate and safest tool to preserve plant germplasm species including plants seeds. In this temperature, physiologic activities and metabolic processes of the seeds and plant members are almost held up and seeds are able to survive at unlimited time periods.In this research, in order to preserve seeds of Silybum marianum medicinal plant, from Astereceae Family in cryopreservation condition, we used three pre-treatments: Glycerol 30 %, and plant vitrification solution 2 and Desiccation for seeds. After a week all of treatments were extracted from the liquid Nitrogen(LN) and were melted in thermae with 42°C. Then seeds were transferred to a germinator in 24°C and 16/8H optical period. Results analyzed statistically through laboratory exploration.Results show, Silybum marianum seeds' survival in LN are maintained in all three treatments among which Desiccation treatment has the highest survival percentage(93%) comparing the control (70%). Mean of seeds' length equals 101/13 mm in vitrification treatment while in control it reaches to 47/38. Thus, Desiccation treatment is recommended as an appropriate alternative to preserve this plant seeds and other Orthodox seeds for long periods of time in preserving centers of plant germplasm preservation. Manuscript profile
      • Open Access Article

        2 - Cryopreservation of Foeniculum vulgare L. seeds
        Nayereh Ahmadi Shahin Mehrpoor
        Cryopreservation is one of the most appropriate and safest tool to preserve plant germplasm species including plants seeds. In this temperature, physiologic activities and metabolic processes of the seeds and plant members are almost held up and seeds are able to surviv More
        Cryopreservation is one of the most appropriate and safest tool to preserve plant germplasm species including plants seeds. In this temperature, physiologic activities and metabolic processes of the seeds and plant members are almost held up and seeds are able to survive at unlimited time periods. In this research, in order to preserve seeds of medicinal plant Foeniculum vulgare L.(Apiaceae) in cryopreservation condition, we used three pre-treatments: Glycerol 30 %, and Plant vitrification solution 2 and Desiccation. After a week all of treated seeds were extracted from the liquid Nitrogen(LN) and were melted in Bain-Marie heater with 42°C. Then seeds were transferred to a germinator in 24°C and 16/8H optical period. Results analyzed statistically through laboratory exploration. Results show, Foeniculum vulgare L. seeds' survival in LN are maintained in all three treatments among which Glycerol 30 % treatment has the highest survival percentage 65%, then Desiccation() and PVS2 with 53% and 15% have germinated, comparing the control 83%. Thus, Glycerol 30 % is recommended as appropriate alternative to preserve this plant seeds for long periods of time in preserving centers of plant germplasm preservation. Manuscript profile
      • Open Access Article

        3 - Investigation of essential oil analysis and antioxidant properties of Foeniculum vulgare Mill. Before and after seed cryopreservation.
        Shahin Mehrpoor Nayereh Ahmadi Fatemeh Jamallou Mohamad Reza Zand monfared
        Introduction: The term "cryopreservation" refers to the storage of cells, organs, and tissues in liquid nitrogen ( 196 ° C). At this temperature, the physical and biochemical reactions of cells are virtually stopped; in these particular circumstances, the time of pr More
        Introduction: The term "cryopreservation" refers to the storage of cells, organs, and tissues in liquid nitrogen ( 196 ° C). At this temperature, the physical and biochemical reactions of cells are virtually stopped; in these particular circumstances, the time of protection is unlimited. Storage of seeds is arguably the most efficient method for the ex situ preservation of plant genetic resources. Low storage costs and regeneration of whole plants from genetically diverse material, offer distinct advantages for the storage for conservation of seeds compared with other types of plant tissues. Method& Material: In order to preserve seeds of Foeniculum vulgare(Apiaceae) medicinal plant, in cryopreservation condition, we used Desiccation pre-treatment for seeds. After a week seeds of treatment were extracted from the liquid Nitrogen and were melted in Ben Murray with 42°C. Then treatment and control seeds were transferred to a germinator in 24°C and 16/8H optical period.The seeds were distillated by Clevenger apparatus and the essential oils obtained were analyzed by GC/MS. The antioxidant activity determined by DPPH method. Result: The percent of viability of the control seeds (83%) was observed in comparison to the treated (53%). The major component of essential oil was anethole (80.04%) comparing the control (78.6%). No adverse effects were observed on the antioxidant properties and their essential oils. Manuscript profile
      • Open Access Article

        4 - Cryopreservation of Cynara scoolymus L. seeds.
        Farideh Saleh Shahin Mehrpoor
        Cryopreservation is one of the most appropriate and safest tool to preserve plant germplasm species including plants seeds. In this temperature, physiologic activities and metabolic processes of the seeds and plant members are almost held up and seeds are able to surviv More
        Cryopreservation is one of the most appropriate and safest tool to preserve plant germplasm species including plants seeds. In this temperature, physiologic activities and metabolic processes of the seeds and plant members are almost held up and seeds are able to survive at unlimited time periods. In this research, in order to preserve seeds of Cynara scoolymus L.medicinal plant, from Asteraceae Family in cryopreservation condition, we used three pre-treatments: Glycerol 30 %, and Plant vitrification solution 2 and Desiccation for seeds. After a week all of treatments were extracted from the liquid Nitrogen(LN) and were melted in thermae with 42°C. Then seeds were transferred to a germinator in 24°C and 16/8H optical period. Results analyzed statistically through laboratory exploration. Results show, Cynara scoolymus L. seeds' survival in LN are maintained in all three treatments among which Desiccation treatment has the highest survival percentage(95%), comparing the control (90%). Mean of seedlings' length equals 53 mm in control while in Glycerol 30 % it reaches to 74.46 mm . Thus, Desiccation is recommended as appropriate alternative to preserve this plant seeds for long periods of time in preserving centers of plant germplasm preservation. Manuscript profile