Cryopreservation of Cynara scoolymus L. seeds.

Subject Areas : botany

Farideh Saleh ¹ , Shahin Mehrpoor ²

1 - دانشجوی کارشناسی ارشد، دانشگاه آزاد اسلامی، واحد قم، دانشکده علوم پایه، گروه علوم گیاهی، قم، ایران
2 - استادیار، دانشگاه آزاد اسلامی، واحد قم، دانشکده علوم پایه، گروه میکروبیولوژی، قم، ایران

Received: 2017-08-01 Accepted : 2017-11-10 Published : 2018-02-20

Keywords: " Cynara scoolymus L. ", Vitrification, Cryopreservation&quot, , &quot, PVS2&quot, ,

Abstract :

Cryopreservation is one of the most appropriate and safest tool to preserve plant germplasm species including plants seeds. In this temperature, physiologic activities and metabolic processes of the seeds and plant members are almost held up and seeds are able to survive at unlimited time periods. In this research, in order to preserve seeds of Cynara scoolymus L.medicinal plant, from Asteraceae Family in cryopreservation condition, we used three pre-treatments: Glycerol 30 %, and Plant vitrification solution 2 and Desiccation for seeds. After a week all of treatments were extracted from the liquid Nitrogen(LN) and were melted in thermae with 42°C. Then seeds were transferred to a germinator in 24°C and 16/8H optical period. Results analyzed statistically through laboratory exploration. Results show, Cynara scoolymus L. seeds' survival in LN are maintained in all three treatments among which Desiccation treatment has the highest survival percentage(95%), comparing the control (90%). Mean of seedlings' length equals 53 mm in control while in Glycerol 30 % it reaches to 74.46 mm . Thus, Desiccation is recommended as appropriate alternative to preserve this plant seeds for long periods of time in preserving centers of plant germplasm preservation.