Cryopreservation of Foeniculum vulgare L. seeds

Subject Areas : botany

Nayereh  Ahmadi ¹ (Botany,Science Department,Qom Azad University, Qom, Iran)
Shahin  Mehrpoor ² (scientific staff)

Received: 2017-06-09 Accepted : 2017-10-31 Published : 2017-02-19

Keywords: cryopreservation, Cryostorage, Foeniculum vulgare, PVS2,

Abstract :

Cryopreservation is one of the most appropriate and safest tool to preserve plant germplasm species including plants seeds. In this temperature, physiologic activities and metabolic processes of the seeds and plant members are almost held up and seeds are able to survive at unlimited time periods. In this research, in order to preserve seeds of medicinal plant Foeniculum vulgare L.(Apiaceae) in cryopreservation condition, we used three pre-treatments: Glycerol 30 %, and Plant vitrification solution 2 and Desiccation. After a week all of treated seeds were extracted from the liquid Nitrogen(LN) and were melted in Bain-Marie heater with 42°C. Then seeds were transferred to a germinator in 24°C and 16/8H optical period. Results analyzed statistically through laboratory exploration. Results show, Foeniculum vulgare L. seeds' survival in LN are maintained in all three treatments among which Glycerol 30 % treatment has the highest survival percentage 65%, then Desiccation() and PVS2 with 53% and 15% have germinated, comparing the control 83%. Thus, Glycerol 30 % is recommended as appropriate alternative to preserve this plant seeds for long periods of time in preserving centers of plant germplasm preservation.