List of Articles yousef doustar

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  1 - The effects of Oleoreupien on prostate and seminal vesicle tissues in streptozotocin induced diabetic rats
  Farzin Javid Esmaeil Safavi Esmaeil Safavi Yoosef Doustar
  10.30495/jvcp.2019.664876
  Diabetes impairs the normal reproductive system function by disrupting spermatogenesis and decreasing sperm count, serum testosterone level and seminal fluid volume. This study aimed to investigate the effects of oleoreupien on tissue changes of prostate and seminal ves More

  Diabetes impairs the normal reproductive system function by disrupting spermatogenesis and decreasing sperm count, serum testosterone level and seminal fluid volume. This study aimed to investigate the effects of oleoreupien on tissue changes of prostate and seminal vesicles in diabetic rats. Thirty male Wistar rats were randomly divided into 3 groups (n=10) including control, diabetic and treatment groups. Rats in the treatment group received Oleoreupien (500mg/kg) for 28 days by oral gavage after induction of diabetes by streptozotocin. At the end of the experimental period, samples of prostate and seminal vesicle were obtained and then slides were prepared and stained by H&E method for histological study. Morphological findings indicated increased secretion of prostate and seminal vesicles, significant increase in diameter of secretory units and significant decrease of interstitial connective tissue in the treatment group compared with the diabetic group. Histomorphometric results also indicated that epithelium height of the prostate and seminal vesicles and diameter of the tubules of the prostate significantly increased in the treatment group compared with the diabetic group (p < /em>˂0.05). The study also revealed that gland weight to body weight in the treatment group increased significantly compared with that of the diabetic group (p < /em>˂0.05). According to current findings, oral administration of Oleoreupien prevented diabetic induced tissue changes in prostate and seminal vesicle in rats. Manuscript profile
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  2 - Effect of regular aerobic exercise on serum levels of cTnI, TNF-α and apoptosis in cardiac ischemia-reperfusion injury of the rats
  یوسف Doustar
  The  process  of  restoring  blood  flow  to  ischemic  heart  muscle  is  antithetically  capable  of inducing cardiac damage. Cardiac troponin I (cTnI) and tumor necrosis factor alpha (TNF-α) are t More

  The  process  of  restoring  blood  flow  to  ischemic  heart  muscle  is  antithetically  capable  of inducing cardiac damage. Cardiac troponin I (cTnI) and tumor necrosis factor alpha (TNF-α) are the important biochemical parameters of cardiac tissue damage. The aim of this study was to evaluate the effects of short term and regular growing long term aerobic exercise on serum levels of cTnI and TNF-α in rats with Ischemia/Reperfusion (I/R) injury. For this purpose, forty male Wistar rats were randomly divided into four equal groups including: control, I/R, I/R with two weeks of aerobic exercise, and I/R with eight weeks of regular growing aerobic exercise groups. Aerobic exercise was performed 5 times per week on treadmill at speed of 10-25m/min for 10-30 minutes with the slope of 5 degrees. For induction of I/R injury, the left descending coronary artery was clamped for 30 minutes, thereafter blood flow was restored for 2 hours. Finally, after collection of blood samples from the retro-orbital plexus for cTnI and TNF-α measurements, all animals were euthanized.  Histologic sections were created for TUNEL staining from the hearts. Regular growing long term aerobic exercise significantly (p<0.05) decreased the cTnI and TNF-α serum levels, which were increased due to I/R injury. Microscopically, the numbers of apoptotic cells were significantly (p<0.01) increased in I/R group compared to the control group. Regular growing long term aerobic exercise significantly decreased the number of apoptotic cells (p<0.05). The results showed regular growing long term aerobic exercise protects the cardiac tissue of rats from I/R injury. Conflict of interest: None declared.   Manuscript profile
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  3 - Histopathological evaluation of the effect of Sulfamide+Trimethoprim administration on renal tissue in broiler chickens
  یوسف Doustar عادل Feizi داریوش Mohajeri M.H Khayat-Nouri M.H Mohammad pour, M.H
  Administration of sulfonamides adversely affects renal tissue in human beings and animals. The aim of the present study was to evaluate the histopathological changes in renal tissue of broiler chickens following administration of Sulfamide+Trimethoprim. In this study, 2 More

  Administration of sulfonamides adversely affects renal tissue in human beings and animals. The aim of the present study was to evaluate the histopathological changes in renal tissue of broiler chickens following administration of Sulfamide+Trimethoprim. In this study, 240 healthy 3 weeks old broiler chickens were randomly assigned to two equal groups. In the treatment groups, sulfonamide +Trimethoprim were administered at a dose of 20ml in 800lit of drinking water for 3 days while no drag was administered in the control group. After 72 hours, tissue samples were collected from the kidneys of the broiler chickens and 5µm thick sections were prepared from specimens fixed in 10% buffered formalin and stained with hematoxylin and eosin and the TUNEL technique. Histopathological examination of the renal tissue in the treatment group indicated the presence of necrosis and apoptosis in renal tubular epithelial cells, congestion, hemorrhage and glomerolopathy. The difference between the treatment and control groups in the severity of renal pathological changes was significant (p<0.001). The result of this study indicates that the drag Sulfamide+Trimethoprim is capable of causing renal tissue damages through induction of apoptosis and necrosis.   Manuscript profile
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  4 - The effect of muscular stresses on the quality of broiler meat
  یوسف Doustar فرید Sarkarati افشین Javadi وحید Haji Abalo
  Collection and transport of chickens from the production farm to the slaughterhouse causes stress. In this study, the relationship between stress causing factors and broiler meat quality was evaluated. Thirty apparently healthy 60-day-old broilers of the same sex and br More

  Collection and transport of chickens from the production farm to the slaughterhouse causes stress. In this study, the relationship between stress causing factors and broiler meat quality was evaluated. Thirty apparently healthy 60-day-old broilers of the same sex and breed were allocated equally to three groups (A and B as treatment and C as the control group). The nutritional and rearing conditions were similar for all groups. Broilers in the first treatment group (A) were subjected to various muscular injuries similar to those occurring during gathering and transfer to abattoir while those in the second treatment group (B) were forced to flap their wings severely. Twelve hours after exertion, blood samples were collected for measurement of creatine phosphokinase (CPK) and total volatile nitrogen (TVN) levels and tissue samples were taken from deep pectoral muscle to prepare microscopic sections in all groups. Macroscopically, swelling, bruising and severe hemorrhage was evident in deep pectoral muscle of the treatment groups, while edema, degeneration, necrosis and infiltration of heterophiles, lymphocytes and macrophages were observed microscopically.  Specific tissue changes were not seen in the control group. The levels of CPK and TVN had increased in treatment groups compared to the control group and their mean differences were also statistically significant (P<0.005).   Manuscript profile
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  5 - Histopathological study of the protective effects of ethanolic extract of Crocus sativus L. (Saffron) stigma on hepatic and renal tissue changes in alloxanized diabetic rats
  داریوش Mohajeri مهران Mesgari Gh Mousavi یوسف Doustar بهرام Amouoghli Tabrizi
  Diabetes mellitus is a metabolic disorder affecting mankind and its incidence is considered to be high all over the world. Hepatic and renal insufficiencies are identified as the most important causes of death in this disease. A multitude of herbs has been described for More

  Diabetes mellitus is a metabolic disorder affecting mankind and its incidence is considered to be high all over the world. Hepatic and renal insufficiencies are identified as the most important causes of death in this disease. A multitude of herbs has been described for the treatment of diabetics throughout the world. The aim of the present study was to assess the protective effects of ethanolic saffron stigma extract (Crocus sativus L.) on early hepatic and renal injuries in alloxan-induced diabetic rats.  For this purpose, 72 male wistar rats were randomly assigned to six different groups of 12 rats each, including healthy control rats, healthy control rats receiving ethanolic saffron extract, mild diabetic (MD) rats, mild diabetic rats receiving ethanolic saffron extract, severely diabetic (SD) rats and severely diabetic rats receiving ethanolic saffron extract. The most effective dose of the extract in reducing blood glucose (40 mg/kg), dissolved in normal saline solution (10 ml/kg) was administered intraperitoneally to treatment groups receiving the extract for 30 consecutive days. Control groups received normal saline solution in the same manner. Four hours after the last extract injections, all rats were euthanized simultaneously by cervical dislocation. Tissue specimens were fixed in 10% buffered formalin and 5 micron thick sections were prepared using routine hitopathological techniques. Histopathology of the liver and kidney in diabetic rats showed a spectrum of changes including periportal degeneration, membranoproliferative glomerulitis, enlargement of lining cells of tubules, lymphocytic infiltration, hyperemia and hemorrhage. These injuries were more prominent in SD rats. The histopathological appearance of liver and kidneys in ethanolic extract treated diabetic rats was near normal. The findings of the present study indicate that ethanolic extract of Crocus sativus L. stigma has protective effects on early diabetic nephropathy and hepatopathy in experimentally induced diabetes. Therefore, ethanolic extract of Crocus sativus L. stigma is recommended for prevention of early renal and hepatic injuries in diabetes mellitus, after randomized clinical trials. Manuscript profile
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  6 - Effects of unilateral uretral obstruction on the induction of renal apoptosis
  یوسف Doustar مهرداد Neshat Garamaleki Ali Rezaie
        Abnormalities of cell numbers are a frequent feature of renal disorders. Programmed cell death is a key factor in the regulation of cell numbers. Apoptosis is an active form of cell death that is modulated by extra cellular lethal and survival More

        Abnormalities of cell numbers are a frequent feature of renal disorders. Programmed cell death is a key factor in the regulation of cell numbers. Apoptosis is an active form of cell death that is modulated by extra cellular lethal and survival signals. Intracellular survival factors also have an important role in the regulation of apoptosis. The aim of this study was to investigate apoptosis of the renal tubular epithelial cells following unilateral uretral obstruction. This study was conducted on 10 dogs allocated equally into treatment and control groups. In the treatment group, unilateral uretral obstruction was created surgically while animals in the control group were subjected to usual nutritional and caring conditions without surgery. After a week, renal tissue samples were collected from both groups. The samples were fixed in 10% buffered formalin and 4-5 micron thick sections were prepared and stained by TUNEL technique. Statistical analysis was performed using the Mann-Whitney U test. The histopathological examination of the treatment group revealed increased apoptosis of renal tubular cells. The average number of apoptotic cells in the treatment group, obtained from calculations made in five different microscopic fields was significantly higher than the control group (P<0.005). This study indicated that canine unilateral uretral obstruction as an animal model can induce apoptosis in renal tubular cells.  Manuscript profile
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  7 - Experimental study of apoptosis in pancreas of SPF chickens following infection by H9N2 serotype of influenza virus
  یوسف Doustar داریوش Mohajeri
       Influenza virus is the cause of cell death in animal and human tissues. Cell death can take place in the form of necrosis or apoptosis. In this study, the type of cell death in the pancreatic tissue of chickens infected by H9N2 serotype of influenza More

       Influenza virus is the cause of cell death in animal and human tissues. Cell death can take place in the form of necrosis or apoptosis. In this study, the type of cell death in the pancreatic tissue of chickens infected by H9N2 serotype of influenza virus (A/chicken/Iran/772/2000) is experimentally investigated. In the present study, 20 SPF chickens with the age of 3- weeks were randomly allocated to two equal groups.  The first group was infected with 0.2 ml of 1:10 dilution and 107.5 EID50 titer of the virus and the second group with equal volume of normal saline solution intranasaly. After 72 hours, specimens were collected from the pancreatic tissue and 5 μm thick microscopic sections were prepared from specimens fixed in 10% buffered formalin and stained with hematoxylin and eosin. Histopathological examination of the pancreatic tissue revealed that there was significant difference in the mean number of apoptotic and necrotic cells between the control and treatment groups (p<0.005). The results of this study indicated that the H9N2 serotype of influenza virus has the potential to induce apoptosis in acinar, alpha and beta islet cells of pancreas. Manuscript profile
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  8 - Experimental study of apoptosis in the prostate tissue following castration
  یوسف Doustar Ali Rezaie صابر Atash Benab
  The Prostate gland is one of the accessory reproductive glands with important physiological functions necessary for successful reproduction. This gland depends on the presence of sex hormones including androgens for its natural function and normal growth and development More

  The Prostate gland is one of the accessory reproductive glands with important physiological functions necessary for successful reproduction. This gland depends on the presence of sex hormones including androgens for its natural function and normal growth and development. So in the case of hyperplasia, hypertrophy or other prostatic disease the most successful and efficient method of treatment is androgenic control that in some cases is unavoidable. The purpose of this study was to investigate the effects of androgenic depletion states by means of castration on the induction of apoptosis in the epithelial glandular cells of the prostate tissue. Two groups of male dogs each containing 5 animals per group were used in this study. The dogs were under observation for 1 month to detect any possible diseases or disorders. After this period the dogs in the treatment group underwent open castration to decrease the levels of the androgenic hormones in the blood while the dogs in the control group were left intact. One week after surgery, the prostate glands of control and treatment animals were collected and used to prepare microscopic sections. The sections were evaluated following staining with TUNEL (TerminaldeoxyNucleotidyl (dUTP) transferase-mediated End Labeling) and H&E methods. The Mann – Whitney U test was used for statistical analysis. Histopathological studies in the treatment group revealed the presence of various forms of apoptotic cells in the glandular epithelium. Average number of apoptotic cells in ten microscopic fields were significantly higher in the treatment group compared with the control group (p<0.005). These results demonstrated that the type of cell death observed in the prostate gland in androgen deprivation states is Apoptosis.   Manuscript profile
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  9 - Immunohistochemical study of β-catenin in experimental colon carcinoma of rat
  یوسف Doustar داریوش Mohajeri فاطمه Fathi Azar علی Namvaran
  Colorectal adenocarcinoma is one of the most prevalent and treatable malignancies of the gastrointestinal tract. Recent studies on colorectal neoplasia indicates β-catenin gene mutation and its intranuclear accumulation inside hyperplastic cells. Therefore, β- More

  Colorectal adenocarcinoma is one of the most prevalent and treatable malignancies of the gastrointestinal tract. Recent studies on colorectal neoplasia indicates β-catenin gene mutation and its intranuclear accumulation inside hyperplastic cells. Therefore, β-catenin may be an important prognosis and diagnostic index of this disease. The aim of this study is to evaluate the nuclear beta-catenin expression in hyperplastic cells of colon following treatment with 1,2-dimethylhydrazine. In this study, 56 wistar male rats with the age of 12 weeks and body weight of 200-300g were selected and randomly allocated into two equal treatment and control groups. In the treatment group, two subcutaneous injections of 1,2-dimethylhydrazine every week at a dose rate of 40 mg/kg were used for 4 weeks to induce colon carcinoma. The control group was given normal saline solution similarly. After 8 weeks, tissues samples of distal colon were collected from both groups for preparation of tissue sections and immunohistochemistry. Immunohistopathological study of samples revealed that nuclear β-catenin expression in the treatment group was significantly higher than the control group (p<0.01). The study indicates that the expression of nuclear beta-catenin can increase in hyperplasia and neoplasia of colon.   Manuscript profile