Veterinary Clinical Pathology The Quarterly Scientific Journal: 5 (8)

Open Access Article

1 - The effect of topical application of autologous blood serum and platelet rich plasma mixture on clinical vision improvement after induction of experimental corneal stromal ulcers in rabbit
رامین Kaffash Elahi S.J Aledavood علی Rezaei

     Corneal ulcers which are mostly due to trauma are among the most common ocular disorders of animals that if not cured properly, might progress to blindness. In this research, the effects of platelet derived rich plasma (PRP) combined with autologous More

     Corneal ulcers which are mostly due to trauma are among the most common ocular disorders of animals that if not cured properly, might progress to blindness. In this research, the effects of platelet derived rich plasma (PRP) combined with autologous blood serum on vision improvement time (attainment of maximum corneal clarity) following creation of deep corneal stromal ulcer was evaluated. For this purpose, 25 adult male New Zealand which rabbits which were allocated to three groups of positive control (10 rabbits), negative control (5 rabbits) and treatment (10 rabbits) were used. In the control corneal regions of each animal, a deep stromal ulcer (extending from the corneal epithelium to the last stromal layer) with a diameter of 4mm was created by means of a probe. Then in the treatment group, pre-prepared PRP was placed immediately on the ulcer and autologous serum drop was administered as 2 drop, 3 times per day for 35 days. In the positive control group Chloramphenicol, Ciprofloxacin and Naphazoline ophthalmic drops and in the negative control group only distilled water were used in the same manner. During the 35 days of the study period, the visual status of the rabbits was evaluated daily. Based on clinical examinations, the treatment group achieved ideal vision significantly faster than the negative and positive control groups and the created ulcer had healed with the least amount of scar production (maximum light penetration from the cornea). Based on the results of this study, the used of PRP is recommended in the treatment of superficial to deep corneal ulcers to obtain faster heading, better vision improvement and least scar production.   Manuscript profile

Open Access Article

2 - Comparison of antibody titer against the infectious bursal disease virus following the disease with that obtained from live intermediate vaccines using indirect hemagglutination (IHA) test in broiler chicks
عادل Feizi مهران Alamdari عبدالله Yadollahi رضا Azmode

       In this study, antibody titer obtained from the outbreak of the infection bursal disease (IBD) was compared with the titer obtained from live intermediate vaccines by indirect haemagglutination (IHA) test in broiler chicks. A total of 450 one More

       In this study, antibody titer obtained from the outbreak of the infection bursal disease (IBD) was compared with the titer obtained from live intermediate vaccines by indirect haemagglutination (IHA) test in broiler chicks. A total of 450 one day old Cobb chicks were divided into 3 groups each containing 150 chicks and were kept for 42 days in the same rearing conditions. Chicks in groups 1 and 2 received Bursin-2 and D-78 vaccines respectively via drinking water n days 14 and 21. The rest of the chicks were kept as the controls (group 4) and did not receive any vaccine against the IBD. Serum samples were collected from all birds 2 weeks after the second IBD vaccination. Additional 150 serum samples were also collected from 3 broiler flocks that were affected by IBD and had a history of vaccination by the previously mentioned method, two weeks after the last clinical sings were observed (group 3). Antibody titer of the samples against the IBD virus were determined by the IHA test and the results were evaluated using ANOVA and SPSS software. The mean antibody titer obtained from Bursin-2 and D-78 vaccines were 3.19 and 3.21 respectively which is less than the titer of 6 needed for protection against the disease. The antibody titer in affected flocks was 7.19. comparison of the mean titer of the two vaccines did not show any significant difference but there was significant difference between the titer obtained from each vaccine and that of the effected flock (p<0.05). The results indicated that the titer obtained from the vaccines the manner that they were employed did not reach protective levels and there is the possibility of disease. Also the efficacy of IHA test in determining antibody titer against the IBD virus was demonstrated.   Manuscript profile

Open Access Article

3 - The effect of chronic administration of ketoconazol on spermatogenesis indices and testis tissue in mice
S.E Safavi M.H Khayat Nouri فرهاد Azimi

     Ketoconazole, a broad spectrum antifungal agent has been employed widely in the treatment of fungal diseases. In addition to being antifungal, studies have indicated that this drug has an inhibitory effect on steroid hormone production including gluc More

     Ketoconazole, a broad spectrum antifungal agent has been employed widely in the treatment of fungal diseases. In addition to being antifungal, studies have indicated that this drug has an inhibitory effect on steroid hormone production including glucocorticoids and sex hormones and also its administration causes reduction in the amount of blood testosterone level and histologic changes in testicular tissue of laboratory animals. The aim of this study is to determine the effect of long term ketoconazole administration on spermatogenesis indices in testicular tissue of mice. In this experimental study 50 male mice were used which were allocated to 5 groups each containing 10 animals. The mice received a 50 mg/kg dose of ketoconazole daily for a period of 15 days, 1, 2 and 3 months orally. One group was used as the control and the other 4 groups received Ketoconazole, testicular tissue samples were collected at the end of the aforementioned time period, and after preparation of tissue sections and staining with hematoxylin and coin the spermiogenesis indices including tubular differentiation index (TDI), spermatogenesis index (SI) and repopulation index (RI) were studied. The results indicated that SI and RI decreased significantly (p<0.05) following 15 days of ketoconazole  administration in comparison with the control group and TDI, SI and RI had also decreased significantly after 1, 2 and 3 months. These results indicate that long term administration of Ketoconazole reduces spermatogenesis and its indices in the testicular tissue of mice probably via reduction in the serumic concentration of testosterone. However the effect of this drug on spermatogenesis and human infertility needs to be further investigated.  Manuscript profile

Open Access Article

4 - Study of the infestation rate of the kidney and spleen of domestic ruminants by Linguatula serrata nymphs in Urmia slaughterhouse
سهراب Rasouli بهادر Hajimohammadi S.S Athari رضا Asadi مهران Mojarrad

     In this study, the prevalence of Linguatula serrata nymphs in kidneys and spleens of 800 domestic ruminants (cattle, buffalo, sheep and goat) in different sexes, ages and seasons was investigated. First, the kidneys and spleens were examined macrosco More

     In this study, the prevalence of Linguatula serrata nymphs in kidneys and spleens of 800 domestic ruminants (cattle, buffalo, sheep and goat) in different sexes, ages and seasons was investigated. First, the kidneys and spleens were examined macroscopically. Then, a digestion method was also applied. Infestation rate in the spleen of cattle, buffalo, sheep and goat were %0/5, %0, %0/5 and %1/5 respectively. No infestation was found in the kidneys. The results of this study shows that the infestation of domestic ruminants to Linguatula serrat nymphs in different sexes and ages were not significant. Also the infestation rate in different seasons was not significant. Manuscript profile

Open Access Article

5 - Ultrastructural study of primordial germ cells, oogonia and oocytes in goat fetus
S.M Banan Khojasteh رضا Ranjbar نعیم Alboghobeish A.A Farshid محبوبه Salehi

     According to morphological evidences, primordial germ cells (PGCs) are derived from the caudal endoderm of the yolk sac and migrate to embryonic gonads. After entering the gonads, first they differentiate to oogonia and then to oocytes. In the presen More

     According to morphological evidences, primordial germ cells (PGCs) are derived from the caudal endoderm of the yolk sac and migrate to embryonic gonads. After entering the gonads, first they differentiate to oogonia and then to oocytes. In the present study, ultrastructure of PGCs, oogonia and oocytes has been examined. Tissue samples were collected from posterior parts of the yolk sacs of fetuses in the early stages of development (with age of less than 1 month), and also gonads of fetuses at later developmental stages (with age of more than 1 month). Samples were studied using transmission electron microscopy after fixation, washing with buffers, dehydration, embedding and staining with uranyl acetate and lead citrate. The Results indicated that PGCs were large with oval to spherical nuclei, reticular chromatin with nucleoli, and there were plenty of glycogen and also different organelles in their cytoplasm. Oogonia showed active mitotic divisions. These cells had regular plasma membranes and were observed as cellular clusters with spherical shape, euchromatin nucleus containing one or more nucleoli, round mitochondria and vacuoles with different sizes in cytoplasm. Oocytes had larger sizes in comparison with oogonia but didn't show cellular clusters. Manuscript profile

Open Access Article

6 - Efficiency of direct microbial diagnosis, IS900 PCR and microbial culture for detection of Mycobacterium avium subsp. Paratuberculosis in the feces of apparently healthy cattle
یونس Anzabi صمد Farashi Bonab Gh.A Moggaddam,

     Johne’s disease or paratuberculosis is a chronic granulomatous enteritis in ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). The disease is responsible for significant economic losses in dairy industry worldwide.&nbsp More

     Johne’s disease or paratuberculosis is a chronic granulomatous enteritis in ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). The disease is responsible for significant economic losses in dairy industry worldwide.  Microbial culture as a golden standard test for detection of MAP in faecal specimens requires 6-16 weeks to complete, whereas accurate and rapid identification of cattle shedding MAP in their feces is essential for successful control of the disease in herds. In the present study, direct microbial diagnosis by Ziehl-Neelsen acid-fast staining, microbial culture on Herrolds’ egg yolk media and two IS900 direct PCR assays were carried out on 100 fecal specimens of apparently healthy cattle collected from dairy herds of Tabriz with a history of Johne’s disease. The number of positive specimens identified by the direct microbial diagnosis, microbial culture and PCR with F90/F91 and FP25/FP26 primes were 7 (7%), 14 (14%), 15(15%) and 25(25%) respectively. These results indicated that PCR detected more positive cases therefore it can be employed for rapid and accurate diagnosis of cattle shedding MAP it their feces and the type of primer used has a significant role in the sensitivity of this test. Direct microbial diagnosis by Ziehl-Neelsen acid-fast staining identified 7 (7%) specimens, two IS900 direct PCR assays identified 15 (15%) and 25 (25%) specimens, respectively, and microbial culture identified 7 (7%) specimens as positive. Collectively, these data indicate that PCR detection of MAP was more sensitive than direct microbial diagnosis by Ziehl-Neelsen acid-fast staining or faecal culture, especially if appropriate primers were used. Manuscript profile

Open Access Article

7 - Electrophoretic study of whey proteins in Holstein cows with clinical and subclinical mastitis by Agarose gel procedure
افشین Davasaz Tabrizi R.A Batavani سیامک Asri Rezaie ملاحت Ahmadi حمید Mirzaie

       Mastitis is one the most important economic diseases in dairy cattle industry, which causes reduction in milk production, treatment expenses, reduction in herd genetic progress and fall in quality of milk. The aim of this study was to ex More

       Mastitis is one the most important economic diseases in dairy cattle industry, which causes reduction in milk production, treatment expenses, reduction in herd genetic progress and fall in quality of milk. The aim of this study was to examine the milk proteins of Holstein dairy cows with different grades of clinical and subclinical mastitis. During the sampling period, none of the cows were in late pregnancy or at early lactation and also had no parasitemia and any other inflammatory diseases.  Clinical and laboratory examinations which were carried out completely revealed the cows were all healthy. They were fed on corn silage, concentrate and alfalfa. In this study, the cows were divided into five groups, each group with 25 cases. For this purpose, milk samples were collected from 125 dairy cattle of two large dairy farms in Tabriz. All the cows were in the lactation period and they were milked three times a day. The groups consist of the control group with negative California mastitis test and negative culture, 2+ subclinical groups, 3+ subclinical group, sub acute clinical group and acute clinical group. The results of the whey electrophoresis using Agarose gel procedure indicated significant difference in albumin levels in all groups except the 2+ subclinical group compared with the control group (p<0.01). There was no difference in beta lactoglobulin and alpha lactalbumin levels in comparison with the control group. The combined levels of alpha and beta globulins in the two groups suffering from clinical mastitis was significantly different from the control groups (p<0.01) but this discrepancy was not significant in the two subclinical groups. In addition, the level of gamma globulins in all mastitis groups was significantly different from the control groups (p<0.01). In conclusion of this study indicated that with the rise in the intensity of infection and inflammation of the mammary tissue, whey proteins especially alpha and beta globulins increase clearly and significantly. Manuscript profile

Open Access Article

8 - Assessment of triacylglycerole level of hepatic cells and total bilirubin and glucose of serum in hybrid dairy cows slaughtered in Ahwaz abattoir
A.P Rezaei Saber محمد Nouri

     Fatty liver is a major metabolic disorder that affects approximately half of multiparous dairy cows mildly or severely in early lactation. The purpose of this study was to compare the hepatic triacylglycerols (TAG) with total bilirubin and glucose of More

     Fatty liver is a major metabolic disorder that affects approximately half of multiparous dairy cows mildly or severely in early lactation. The purpose of this study was to compare the hepatic triacylglycerols (TAG) with total bilirubin and glucose of serum. For this purpose, 203 blood and liver samples were collected from the indigenous hybrid cows immediately after slaughtered. The sampled cows were divided into four groups according to their pregnancy status: 1-8 months pregnant, 8-9 months pregnant, less than a month after parturition and more than a month after parturition. The percentage of TAG in liver samples and the amounts of total bilirubin and glucose in blood were measured. All liver samples collected in this study showed percentage of fatty infiltration. The accumulation of fat in the liver of cows that had recently given birth was significantly (p<0.001) more than the other groups and the fat content returned to pre-parturition levels after a month. There was also a significant difference in the amount of serumic total bilirubin of hepatic samples with different fat percentage (p<0.001). Serumic glucose levels were significantly different between the four treatment groups (p<0.05). From the 203 samples collected in this study, 1% was considered as healthy, 44.30% as affected by mild fatty liver, 51.2% as moderate fatty liver and 3.4% as having severe fatty liver. The results also indicated that among the cows studied, fatty liver is prevalent especially in the first month after parturition Manuscript profile

Open Access Article

9 - Rabies and its present situation in Iran
M.Gh Nadalian حسن Tadjbakhsh M.R Mokhber Dezfuli Ali Rezakhani سوسن Simani محمود Bolourchi

     Rabies is a dangerous infections disease which is highly fatal and zoonotic. The disease occurs in many countries of the world and in Iran, rabies is endemic and a major public health Problem. Most warm blooded animals are susceptible to rabies. It i More

     Rabies is a dangerous infections disease which is highly fatal and zoonotic. The disease occurs in many countries of the world and in Iran, rabies is endemic and a major public health Problem. Most warm blooded animals are susceptible to rabies. It is transmitted by the bite of an infected animal to humans and other animals. The saliva of the sufferers is a rich source of rabies virus. Rabies is not seen in countries such as Scandinavia, Britain, Ireland, Scotland, Australia and New Zealand which is related to their geographical situation and being surrounded by water. But in countries like the United States of America, Canada, Europe and Iran the rabies is endemic. Dogs, wild carnivores especially wolves and foxes are the main transmitters of the disease. Rabies has been known approximately from the year 2300 BC with the dog considered as the main vector. Scientists like Avicenna and Sayyed Esmail Jorjani have described rabies. Louis Pasteur presented antirabies treatment and vaccinations in 1885. The Pasteur institute of Iran opened in 1920 and today there are more than 300 antirabies treatment centers in the country involved with the treatment rabies. More than 100000 persons each year in Iran are treated for rabies due to being bitten by animals especially dogs suspected of having rabies. According to the report of the Pasteur institute of Iran, 421 Positive cases of rabies of which 3 were human cases where recorded in the year 2001. These figures for 2002 to 2004 were 356 positive cases with 6 human cases, 314 positive cares with 10 human cases and 325 positive cases with 5 human cases respectively. On average, 6 human cases of rabies were recorded each year with all leading to death. Considering the increasing trend of animal bites and the number of animals and humans affected by rabies the responsible authorities including the National Veterinary Organization, Ministry of health, municipalities, Ministry of Interior and … must present effective and practical measures for vaccination of dogs, destruction of stray dogs and control and vaccination of susceptible wild animals to rabies and they should take the matter seriously to minimize this zoonotic disease and public health and economic problem.          Manuscript profile

Open Access Article

10 - A case report of severe conjunctival edema due to traumatic pericarditis in a 10 months old Holstein calf
احسان Torki بابک Sajadi امیر Zeidabadi محدثه Maghamipour سولماز Naserli فاطمه Ghasemi S.H Hassas

     A 10 months old Holstein calf with a history of anorexia and visual system disorders was referred to the Veterinary Medical and Teaching Hospital of TehranUniversity. On Clinical examination body temperature of 34.8oc and respiratory rate of 33 per m More

     A 10 months old Holstein calf with a history of anorexia and visual system disorders was referred to the Veterinary Medical and Teaching Hospital of TehranUniversity. On Clinical examination body temperature of 34.8oc and respiratory rate of 33 per minute were recorded. Severe edema was observed in the intermandibular space, brisket, vagina and conjunctiva and the jugular veins were bilaterally distended and pulsatile. Auscultation of the heart revealed muffled heart sound and decreased amplitude of P, QRS and T waves were observed from the electrocardiogram was performed from the left fifth intercostals space and fibrinous exudate was withdrawn. Severe conjunctival edema of this calf was due to cardiac insufficiency.         Manuscript profile

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