Veterinary Clinical Pathology The Quarterly Scientific Journal: 4 (15)

Open Access Article

1 - Comparison of apoptosis in canine transmissible veneral tumor (TVT) pre and post chemotraoy with vincristine sulphate
یوسف Doustar داریوش Mohajeri رامین Kafash Elahi

The canine transmissible veneral tumor (CTVT) is a prevalent tumor in canidae. It is transmitted by coitus, forming multiple neoplastic masses on the external genitalia of both sexes within the family canidae. CTVT have an aberrant karyotype and the origin of the neopla More

The canine transmissible veneral tumor (CTVT) is a prevalent tumor in canidae. It is transmitted by coitus, forming multiple neoplastic masses on the external genitalia of both sexes within the family canidae. CTVT have an aberrant karyotype and the origin of the neoplastic cells is undetermined but immunophenotyping suggests that the tumor has a histocytic origin. In this study 10 dogs with canine transmissible veneral tumor were selected and received vincristine sulphate (0.025 mg/kg/b.w) chemotrapy to induce apoptosis in neoplastic cells. Biopsy specimens were collected from tumors during the growth phase, before and again after chemotherapy from the same dogs. The specimens were fixed in 10% formalin and then prepared routinely for H&E and TUNEL assays. Histopathological study of tissue section of CTVT before chemotherapy revealed sheets of uniform neoplastic cells, round to oval in shape with defined cytoplasmic border. There were a few TUNEL positive cells and mitotic figures. In tumor specimens after chemotherapy increased TUNEL positive cells and depilation of neoplastic cells in stroma of tumor were observed. Mean deference of histopathological changes and TUNEL positive cells before and after chemotherapy were significant (p<0.003). This study indicates that vincristine sulphate is capable of induction of apoptosis in neoplastic cells of CTVT. Manuscript profile

Open Access Article

2 - Effect of some probiotics on Salmonella paratyphi during associated growth in milk
حمید Mirzaei افشین Javadi یوسف Angouri

      The aim of this study is to determine the effects of Lactobacillus acidophilus, Lactobacillus casei, Bifidobacterium angulatom and Bifidobacterium bifidum on salmonella paratyphi in associated condition in milk. At first a quantity of 1.5 &time More

      The aim of this study is to determine the effects of Lactobacillus acidophilus, Lactobacillus casei, Bifidobacterium angulatom and Bifidobacterium bifidum on salmonella paratyphi in associated condition in milk. At first a quantity of 1.5 × 108 cfu/ml activated S. paratyphi was added to 500 ml of sterile milk and after homogenization distributed equally in five erlene meyers. The first erlene meyer was considered as individual culture and in to the second, third, fourth and fifth erlene meyer a quantity of 1.5×108 cfu/ml of the above probiotic was inoculated respectively and after 24-48 hours of incubation at 37 oC, pH was measured by pH meter and S. paratyphi count was determined by pour plate method in SSA medium. This procedure was repeated 10 times and the mean of pH and number of S. paratyphi in one ml of individual culture and with probiotic cultures was compared using statistical analysis.  According to statistical tests of ANOVA and Tukey at α=0.05, in 24 and 48 hour incubation of associated growth of L. acidophilus, L. casei and B. bifidum had significant inhibitory effect on S. paratyphi growth (p<0.01). However, in similar condition associated growth of L. angulatum did not show significant inhibitory effect on S. paratyphi growth. Meanwhile, in 24 and 48 hour incubation of associated growth of L. acidophilus, L. casei and B. bifidum reduced the mean of pH of milk samples compared to control sample significantly (p<0.01). These results show the consumption of probiotic products containing B. angulatom, B. bifidum, L. acidophilus and L. casei could have beneficial effects in prevention of Salmonella paratyphi infection. Although further research especially at in vivo condition in this aspect should be carried out. Manuscript profile

Open Access Article

3 - Comparison of the serumic levels of vitamin A, vitamin C and zinc between apparently healthy and those affected by febrile pneumonia in Ghezel sheep
محمد Mashayekhi M.H Khayat Nouri A.R Ebadi فرامرز Panahi

The aim of this study was to determine the correlation between subclinical deficiency of Vit A, Vit C and zinc and ovine pneumonia. This study was conducted on 400 sheep affected by pneumonia and 100 apparently healthy sheep. All animals were from the Ghezel breed with More

The aim of this study was to determine the correlation between subclinical deficiency of Vit A, Vit C and zinc and ovine pneumonia. This study was conducted on 400 sheep affected by pneumonia and 100 apparently healthy sheep. All animals were from the Ghezel breed with the age of 0.5-2 years and body weight of 35-60 kg. Sampling was done during the summer and autumn in Miandoab province. Patients showing signs of rapid and shallow breathing, dyspnea, coughing, nasal discharge and abnormal lung sound were selected as having pneumonia. Animals without any sign of particular disease were selected as apparently healthy after general examination. Blood samples were collected from the jugular vein. The results indicated that serum levels of vitamin A, vitamin C and zinc were 46.94±0.26 (μg/dl), 1.83±0.05 (mg/dl) and 183.25±1.86 (μg/dl) in healthy sheep respectively and serum levels of vitamin A, vitamin C and zinc were 42.45±0.19 (μg/dl), 1.47±0.02 (mg/dl) and 134.59±1.77 (μg/dl) in pneumonic sheep (p=0.000) respectively. It can be concluded that there is a significant correlation between subclinical deficiency of vitamin A, vitamin C and zinc serum and ovine pneumonia Manuscript profile

Open Access Article

4 - The effect of intramuscular administration of medroxy progesterone acetate (MPA) on electrocardiographic alterations of female dogs
مهرداد Neshat Gharamaleky M.H Khayat Nouri بهرنگ Eslami سیامک Mashhadi Rafie

Progestins are useful for hormonal prevention of pregnancy in humans and animals. Progestrone and medroxy progestrone acetate are used for treatment of premenopausal syndrome and decreasing complications of estrogen therapy after menopause. Prescription of progesterone More

Progestins are useful for hormonal prevention of pregnancy in humans and animals. Progestrone and medroxy progestrone acetate are used for treatment of premenopausal syndrome and decreasing complications of estrogen therapy after menopause. Prescription of progesterone in dogs is usually done to control or delay estrus. If this drug is used at anestrous it will inhibit the gonadotropin excretion beyond the basic level and it will prevent cycle from happening again in proestrus or at the beginning of estrus progesterone will prevent ovulation. Use of progesterone is not totally safe in bitches. It can cause several problems such as cystic endometrial hyperplasia and pyrometera. In this study the effects of medroxy progestrone acetat (MPA) on electrocardiographic changes are evaluated. After nutritional and environmental uniformity and other evaluations, the animals were divided into two groups with six bitches in each group. MPA was used at 10 mg/kg for the first group and 20 mg/kg for the second group. An ECG was recorded from the first group in time zero. MPA was injected intramuscularly and immediately after ECG recording and the time of injection was noted. Another ECG was recorded 0.5 and 2 hours after injection. The same process was repeated for the second group. It was observed that MPA at 20 mg/kg increased the heart rate and consequently PR interval and RR interval were decreased significantly in normal range. Also administration of drug at 10 mg/kg decreased the heart rate and increased PR interval, QT interval and RR interval significantly in normal range. It is probable that these observations are the result of MPAs dose dependent vasodilatory nature that induces the heart to exhibit compensative reactions such as increasing heart rate. Manuscript profile

Open Access Article

5 - Study of sedative, preanaesthetic and anxiolytic effects of herbal extract of Lavandula stoechas in comparison with diazepam in rat
علی Rezaie بهبود Jafari محمد Jalilzadeh

Lavandula stoechas grows naturally in most parts of the world specifically south France, the Mediterranean region and Torento. The plant has various pharmacological properties including analgesic, anti-inflammatory, antidepressant, hypnotic, sedative and tranquilizer, m More

Lavandula stoechas grows naturally in most parts of the world specifically south France, the Mediterranean region and Torento. The plant has various pharmacological properties including analgesic, anti-inflammatory, antidepressant, hypnotic, sedative and tranquilizer, muscle relaxant, anticonvulsant, antibacterial and antispasmodic. For studying the effectiveness of sedative, preanesthetic and anxiolytic effects of Lavandula  stoechas in comparison with diazepam different groups of female Wistar rats with the same age and weight conditions received intraperitoneal injections of Lavandula  stoechas (100, 200, 400 mg/kg, ip), diazepam (1.2 mg/kg, ip), dimethyl sulfoxide (DMSO) as a placebo with equal volume 30 minutes before assessing the sedative and preanesthetic effects (induced sleep duration by ketamine, 40 mg/kg, ip) and anxiolytic effects (using Elevated plus maze) and (Rotarod test). Statistical analysis of the results obtained represent a significant increase in sleep time induced with ketamine and also a significant increase in the time the rats spent in open arms of maze with high and low doses of Lavandula stoechas herbal extract (p<0.01). The result showed that herbal extract of Lavandula stoechas at 200 mg/kg has sedative, preanesthetic and anxiolytic effects. Manuscript profile

Open Access Article

6 - Efficacy of Sodium Bentonite, Esterified Glucomannan and Humic Acid to counteract the effects of Aflatoxicosis on renal pathological changes and serum biochemical parameters in Broilers
حسن Ghahri A.H Soleymannezhad داریوش Mohajeri افشین Zakeri

This study was conducted to evaluate the renal pathological changes and serum biochemical parameters in broilers fed a diet containing low-levels (254 ppb) of aflatoxin (AF) and adsorbents until 35 days of age. Seven-day-old male broilers were randomly assigned to one o More

This study was conducted to evaluate the renal pathological changes and serum biochemical parameters in broilers fed a diet containing low-levels (254 ppb) of aflatoxin (AF) and adsorbents until 35 days of age. Seven-day-old male broilers were randomly assigned to one of the nine dietary treatments with four replicates of 12 chicks each. Treatments were: 1) control; 2) naturally AF contaminated diet (NCD); 3,4,5,6 and 7) NCD supplemented with 0.2%, 0.4%, 0.6%, 0.8% and 1%, humic acid (HA) respectively; 8 and 9) NCD supplemented with 0.5% sodium bentonite (SB) and 0.1% esterified glucomannan (E-GM), respectively. Blood samples were taken on the 35th day of the study and the phosphorus, calcium, urea nitrogen (BUN), and creatinine levels in serum were analyzed. When the chicks reached 35 days of age, the feeding trial was terminated and 12 broilers from each treatment were selected at random and were killed for pathological examination. Microscopically, the kidney in chickens fed diet containing 254ppb AF showed significant lesions, compared to control. At the end of the study, it was determined that aflatoxin caused a decrease in BUN and an increase in phosphorous levels. The addition of HA and E-GM to NCD decreased both the alterations of serum biochemical parameters and the severity of lesions in the kidney. These results clearly demonstrated that histological lesions and serum biochemical changes were observed in chickens fed a diet containing 254ppb AF and that addition of dietary E-GM and HA is effective in counteracting the toxic effects of naturally contaminated feed with mycotoxins. Manuscript profile

Open Access Article

7 - The study of infection rate to Bovine Herpes Virus – 1(BHV-1) in milk samples of dairy cattle in Urmia and Maku by ELISA method
احمد Gharekhani احمد Morshedi

A cross sectional study was performed to find out and compare the infection rate to BHV-1 of dairy cattles in Urmia and Maku. Random sampling in three age groups of (3-4, 5-6 and 7 years and more) was done. In every region 22 cattle herds were selected, from each cattle More

A cross sectional study was performed to find out and compare the infection rate to BHV-1 of dairy cattles in Urmia and Maku. Random sampling in three age groups of (3-4, 5-6 and 7 years and more) was done. In every region 22 cattle herds were selected, from each cattle herd 10 cows were picked out and a sample of milk was collected from each cow. Individual milk samples were investigated by indirect ELISA method in order to determine the presence of BHV-1 antibodies in the sample. By determining the OD of the samples and the percentage of positive and negative ELISA, the results were compared. Data analysis was performed by SPSS (Ver.16) and S-plus 2000 software with Mann-Whitney, Chi- square and Kruskal-Wallis tests to find out the difference between the average infection in the two regions and the difference between the total average of infection rate between the age groups (p<0.05). The results showed that the rate of infection in Maku was 34/08% (with 75 positive milk samples) and in Urmia it was 13/04% (with 29 positive milk samples). It shows that the rate of infection in Maku is much higher to the extent that a significant difference in the infection rate between the two regions is observed. Also upon the study of the total average of infection rate between the age groups of the two regions the rate of infection cases in the first group (32%) was meaningfully higher than the other two age groups amounting to 14% and 6% respectivety.                                               Manuscript profile

Open Access Article

8 - Investigating the effects of using Nettle (Urtica dioica ), Menta pulagum (Oreganum valgare) and Zizaphora (Thymyus valgaris) medicinal plants on performance, carcass quality, blood biochemical parameters and blood cells of broilers
عباس Heydari علی Nobakht A.R Safamehr سامان Mahdavi

This experiment was conducted to evaluate the effects of using Nettle,Menta pulagum and ‌‌Zizaphora medicinal plants on performance, carcass quality, blood biochemical parameters and blood cells of broilers. The experiment was conducted in a completely randomized design More

This experiment was conducted to evaluate the effects of using Nettle,Menta pulagum and ‌‌Zizaphora medicinal plants on performance, carcass quality, blood biochemical parameters and blood cells of broilers. The experiment was conducted in a completely randomized design with 288 broilers (Ross-308) in 8 treatments and 3 replicates (with 12 birds in each replicate) from 1 to 42 days and included: 1) control group without using any medicinal plants, 2) 1.5% of ‌‌Nettle, 3) 1.5% of Menta pulagum, 4) 1.5% of Zizaphora, 5) 1.5% of Nettle and Menta pulagum, 6) 1.5% of  Nettle and Zizaphora, 7) 1.5% of Menta pulagum and ‌‌Zizaphora, 8) 1.5% of Nettle,Menta pulagum and ‌‌Zizaphora. The results showed that using these medicinal plants and their mixtures had significant effects on performance, carcass traits and blood biochemical parameters of broilers (p<0.05). The highest amount of daily feed intake (89.55 g) was observed in the control group and the lowest (80.11g) in experimental group 6, the lowest percent of abdominal fat (3.03) was observed in experimental group 5, the highest percent of breast (33.54) was observed in experimental group 7, the lowest level of blood cholesterol (100.97 mg.dl) was observed in experimental group 3 and the lowest blood triglyceride (38.37 mg,dl) was observed in experimental group 8. The overall results showed that using Nettle,Menta pulagum and ‌‌Zizaphora medicinal plants and their mixtures have positive effects on performance, carcass traits and blood biochemical parameters of broilers. Manuscript profile

Open Access Article

9 - Effect of dietary supplementation of licorice extract on egg quality and performance of hens
محمد Sedghi ابولقاسم Golian پریسا Soleymani

The objective of this study was to determine the effects of various levels of dietary licorice extract on egg production, egg weight, specific gravity, feed conversion ratio, egg shell quality and blood parameters. One hundred and twenty eight laying hens (58 weeks old) More

The objective of this study was to determine the effects of various levels of dietary licorice extract on egg production, egg weight, specific gravity, feed conversion ratio, egg shell quality and blood parameters. One hundred and twenty eight laying hens (58 weeks old) were used in a completely randomized block design to test four diets with 4 replicates of 8 birds each. The four diets were supplemented with 0, 2, 4 or 6 g/kg of diets licorice extract. Hens fed the diet supplemented with 4 g/kg of licorice extract had greater (p<0.06) egg production than the control fed diet during the experiment. Hens fed 4 g/kg of licorice extract produced a significantly thicker shell (p<0.05) than those fed supplemented with 6 g/kg (389 vs. 374 mm). Percentage of abdominal fat pad was significantly decreased (p<0.05) in birds fed diet containing 6 g/kg of licorice extract compared to control diet (8.3 vs. 14.9 percentage of carcass weight). Feed consumption, feed conversion ratio, egg weight, dry shell weight, egg-specific gravity, percentage of wet albumen and wet yolk based on percentage of whole egg weight and organ weight were not influenced by licorice supplementation. This study showed that licorice extract at the level of 4 g/kg would positively influence egg production or shell quality and decrease the abdominal fat pad when 6 g/kg of licorice was fed to hens. Manuscript profile

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