List of Articles Amir Ali Kaveh

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Antibiotic resistance pattern and presence of biofilm producing ica operon virulence genes in coagulase positive Staphylococcus aureus isolated from bovine mastitis in East Azerbaijan province
  Saeed Salehi Younes Anzabi kaveh Amir ali
  10.30495/jvcp.2021.1937204.1317
  Staphylococcal mastitis is one of the most important diseases in industrial livestock and Staphylococci capable of producing biofilm, micro and macro abscesses, are considered to be the cause of malignant mastitis which result in culling of dairy cattle. The ability of More

  Staphylococcal mastitis is one of the most important diseases in industrial livestock and Staphylococci capable of producing biofilm, micro and macro abscesses, are considered to be the cause of malignant mastitis which result in culling of dairy cattle. The ability of these bacteria to produce biofilm depends on the presence of ica operon genes (icaA, icaB, icaC, icaD and icaR) and also some environmental factors. In the present study, the pattern of antibiotic resistance and the presence of operon ica virulence genes in Staphylococcus aureus isolated from bovine mastitis was investigated. According to the findings of microbiological and simplex PCR tests, it was determined that in the farms tested in East Azerbaijan province, Staphylococcal mastitis has a relatively high prevalence and among the relevant isolates, a relatively high percentage of them contain a variety of operon ica virulence genes that encode the ability to produce biofilm. Also, the results of antibiotic susceptibility testing performed on these isolates showed that they were more resistant to most of the antibiotics tested. Due to the high presence of ica operon genes, especially genes such as icaA and icaD, which result in production of stronger and excessive biofilm as well as increased resistance to various antibiotics, the results of the present study therefore indicate an unfavorable prognosis regarding the success of antibiotic treatment of staphylococcal mastitis in livestock farms in the region. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - Evaluation of cardiac injury biomarkers in cattle with acute clinical mastitis
  meysam fllah majid fartashvand hossein kouchakzadehomran amirali kaveh
  Abstract    This study was carried out on 30 Holstein dairy cattle with acute clinical mastitis (ACM) and 30 healthy ones. After confirmation of ACM through clinical examination, venous blood samples were collected and cardiac troponin I (cTnI) was measured u More

  Abstract    This study was carried out on 30 Holstein dairy cattle with acute clinical mastitis (ACM) and 30 healthy ones. After confirmation of ACM through clinical examination, venous blood samples were collected and cardiac troponin I (cTnI) was measured using chemiluminescence assay. Cardiac enzymes activities including CK-MB, AST and LDH were analyzed with special kits and spectrophotometric method. According to the findings mean heart rate (p=0.001), respiratory rate (p=0.026), and rectal temperature (p=0.030) were significantly increased in diseased group. cTnI level was 1.018 ± 0.235 ng/ml in cattle with ACM, which was significantly higher than healthy cattle (0.011±0.006 ng/ml; p=0.000). Other cardiac biomarkers were increased in diseased group, however elevation of serum activities of AST (p=0.047) and CK-MB (p=0.000) were statically significant. Although serum LDH activity in diseased group was higher than control group; but this difference was statistically non-significant (p=0.454). There were significant positive correlations between cTnI concentration with heart rate (p=0.018; r=0.853), respiratory rate (p=0.024; r=0.671), and rectal temperature (p=0.038; r=0.542). Heart rates were significantly correlated with serum activities of CK-MB (p=0.047; r=0.722) and AST (p=0.035; r=0.649). These results indicate some degree of heart damage caused by acute clinical mastitis in dairy cattle. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  3 - Effects of Inositol on the protoplasmic droplet detachment and motility patterns of epididymal tail sperms in the water buffalo
  A.A Kaveh پرویز Tajik حمید Ghasemzadeh_Nava کیوان Abdi پژمان Mirshokraie
       The purpose of this study is to determine the effects of 1Normal concentration of Inositol solution on the protoplasmic droplet detachment and motility patterns of epididymal tail sperms in the water Buffalo. Thus, 50 pairs of adult male water buffal More

       The purpose of this study is to determine the effects of 1Normal concentration of Inositol solution on the protoplasmic droplet detachment and motility patterns of epididymal tail sperms in the water Buffalo. Thus, 50 pairs of adult male water buffalo testicles were collected immediately alter slaughter during March to September 2007 from urmia abattoir and transported to the laboratory alongside 4ºC ice pack in 30 minutes. Epididymal tail sperms were collected by incising the hypo vascularized areas and transported to Hams F10 culture media with normal sperm pH (6.7-7.4) and containing 10% embryonic cell serum and following 15 minutes of incubation at 37oC inside the Co2 incubator, 1N solution Inositol was added to sperm containing microtubes. Eosin-nigrosin stained sperms were then prepared from the above mentioned samples at 15, 30, 45, 60, 90, 120, 180, 240 and 360 minutes and the motility patterns were investigated using the CASA software. The number of live and dead sperms and sperms with or without the proximal and distal protoplasmic droplets were counted in the stained sperms. The results obtained from the CASA software were analyzed using t-Test. The results indicated that 1N Inositol had no significant effect on sperm motility patterns but had significantly (p<0.05) increased the number of live sperms without the protoplasmic droplet at times 30, 120, 180 and 240. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  4 - The effect of different concentrations of citric acid on motility patterns of bovine epididymal sperms in Hams F10 milieu
  کیوان Abdy پرویز Tajik حمید Gasemzade A.A Kave پژمان Mirshokraei
      The aim of this study was to investigate the effect of three concentration of citric acid on motility patterns of bovine epididymal sperms. For this purpose, 50 pairs of bovine testicles were collected immediately after slaughter form urmia abattoir a More

      The aim of this study was to investigate the effect of three concentration of citric acid on motility patterns of bovine epididymal sperms. For this purpose, 50 pairs of bovine testicles were collected immediately after slaughter form urmia abattoir and transferred to the laboratory alongside 5oc ice pack. Epididymal tail sperms were collected with a few incisions in vascular areas and transferred to hams f10 milieu with 10% fetal calf serum and counted after 15 minutes of incubation at 37oc in Co2 incubator. Concentrations of 50 million sperms per ml were proposal and in the normal sperm pH rang of 6.7-7.4, 0.1, 0.2 and 0.3 normal concentration of citric acid were added to sperm continuity micro tubes (normal concentration of acid equals 7 mg/ml of bovine serum) and at 15, 30, 45, 60, 90, 120, 180, 240 and 360 minutes the motility patterns of epididymal sperms were evaluated using the computer assisted sperms analyzing (CASA) method. Data were analyzed with one-way ANOVA using the SPSS 15 software. The results indicated significant differences in various indices of sperm motility patterns (Curvilinear   Velocity, Straight-line Velocity, Average Path Velocity, Mean Angel Degree, Amplitude of Lateral Head Displacement, Beat-Cross Frequency, Linearity, Wobble) particularly at 0.3 normal concentration of citric acid compared with the control. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  5 - Evaluation of cardiac troponin I alterationsin dairy cattle with septicmetritis
  majid fartashvand ali derangian amirali kaveh
  Metritis is an important disease in dairy cattle which causes economical loses including decrease in milk yield, increase calving interval, treatment costs and death of ill cases. Septic metritis usually occurs within 2-10 days after parturition, and characterized clini More

  Metritis is an important disease in dairy cattle which causes economical loses including decrease in milk yield, increase calving interval, treatment costs and death of ill cases. Septic metritis usually occurs within 2-10 days after parturition, and characterized clinically with sever toxemia associated with purulent odorous uterine discharge with or without retained placenta. In this study, serum levels of cTnI were measured in 50 female Holstein cattle with septicmetritis and compared with normal cows. cTnI of serum in disease and control groups were 0.017 ± 0.008 and 0.005 ± 0.000 ng/dl, respectively. Heart rate, respiratory rate and rectal temperature in disease cases were significantly higher than normal cattle. There was significant correlation with cTnI and heart rate and rectal temperature. Endotoxemia is one of possible reasons of elevation of serum cTnI. Cytokines and endotoxins originated from gram negative bacteria that cause myocardium depression and ventricular dilatation. Furthermore impairment of left ventricle function is a significant effect of septic shock.   Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  6 - Evaluation of the presence of anti zona pellucida Antibodies is the serum of repeat breeder Holstein dairy cattle
  amirali kaveh hossein nikniyaz
     This study was conducted to evaluate the presence of anti zona pellucida antibodies in the serum of  repeat breeder Holstein cows in Third station Dairy Cattle Husbandry Agro Industry  in Moghan. The study was conducted on 168 non-pregnant cows wi More

     This study was conducted to evaluate the presence of anti zona pellucida antibodies in the serum of  repeat breeder Holstein cows in Third station Dairy Cattle Husbandry Agro Industry  in Moghan. The study was conducted on 168 non-pregnant cows with a history of Repeat breeder. Animals were selected in two age groups ¸ more than three times calving or less. Ten ml of blood were obtained from Animals from the jugular vein and serum was separated. The presence of anti-ZP antibodies in animal sera were tested by commercial test kits for ELISA (Bioserve Diagnostic, Zona pellucida antibody ELISA, BS-20-20, Germany). From a total of 168 tested animals, 13 animals tested positive (7.74%) and 155 animals were negative (92.26%). Five of 109 cattle with less than three calving were positive and 8 of 59 animals with more than three calving were positive. The results showed highly significant differences between the two groups (Z=2/07) and this means that increasing age has a significant effect on the rate of appearance of anti zona pellucida antibodies in the serum of these animals. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  7 - Comparison between epidural injection of calcium–naloxone and intramuscular GnRH as therapeutic methods in the treatment of follicular ovarian cysts in dairy cattle
  amirali kaveh saeid Rezaye Haghdoust samad mosaferi
     Ovarian cysts is one of the problems of dairy cattle with high production. The aim of this study was to evaluate the response to treatment of ovarian follicular cysts using calcium naloxone comparison with the conventional method of intramuscular GnRH injec More

     Ovarian cysts is one of the problems of dairy cattle with high production. The aim of this study was to evaluate the response to treatment of ovarian follicular cysts using calcium naloxone comparison with the conventional method of intramuscular GnRH injection. This study was conducted in third station dairy cattle farming center in Moghan on 54 cows' affected follicular ovarian cysts approximately 100 to 150 days after parturition. Following physical examination and diagnosis of the cystic cows, the animals were divided into three groups. The first group was the control group (5ml GnRH IM), the second group was the treatment group 1 (Nx via epidural injection 2 times with 3 day intervals) and the third group was the treatment group 2 (Ca–Nx via epidural injection 2 times with 3 day intervals and 5 ml of intramuscular GnRH simultaneously with the second Ca-Nx injection). To check the results, the animals was examined two weeks after the last injection. The results indicated that there was a significant difference in luteinization rate between the first and second groups with more luteinization rate in the first group. There was no significant difference between the first and third group in luteinization rate. Also, there was a significant difference in luteinization rate between the second and third group, with more luteinization rate observed in the third group. There was no significant difference between the fertility rate of the treatment and control groups. Manuscript profile