The effect of different concentrations of citric acid on motility patterns of bovine epididymal sperms in Hams F10 milieu

Subject Areas : Veterinary Clinical Pathology

کیوان Abdy ¹ , پرویز Tajik ² , حمید Gasemzade ³ , A.A Kave ⁴ , پژمان Mirshokraei ⁵

1 - Graduate of Veterinary Reproduction & Obstetrics, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science & Research Campus, Tehran, Iran
2 - Department of Clinical Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 - Department of Clinical Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4 - Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
5 - Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran

Received: 2008-10-30 Accepted : 2009-01-19 Published : 2008-11-21

Keywords: citric acid, Cow, Epididymal sperm, CASA- Hams F10 milieu,

Abstract :

    The aim of this study was to investigate the effect of three concentration of citric acid on motility patterns of bovine epididymal sperms. For this purpose, 50 pairs of bovine testicles were collected immediately after slaughter form urmia abattoir and transferred to the laboratory alongside 5oc ice pack. Epididymal tail sperms were collected with a few incisions in vascular areas and transferred to hams f10 milieu with 10% fetal calf serum and counted after 15 minutes of incubation at 37oc in Co2 incubator. Concentrations of 50 million sperms per ml were proposal and in the normal sperm pH rang of 6.7-7.4, 0.1, 0.2 and 0.3 normal concentration of citric acid were added to sperm continuity micro tubes (normal concentration of acid equals 7 mg/ml of bovine serum) and at 15, 30, 45, 60, 90, 120, 180, 240 and 360 minutes the motility patterns of epididymal sperms were evaluated using the computer assisted sperms analyzing (CASA) method. Data were analyzed with one-way ANOVA using the SPSS 15 software. The results indicated significant differences in various indices of sperm motility patterns (Curvilinear   Velocity, Straight-line Velocity, Average Path Velocity, Mean Angel Degree, Amplitude of Lateral Head Displacement, Beat-Cross Frequency, Linearity, Wobble) particularly at 0.3 normal concentration of citric acid compared with the control.

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