• Home
  • Alireza Iranbakhsh
  • OpenAccess
    • List of Articles Alireza Iranbakhsh

      • Open Access Article

        1 - Effect of Microgravity on growth, Ultrastructure, phenol, flavonoid, and inhibition of DPPH radical in Hyoscyamus niger L. Seedlings
        Rogjayeh Pourhabibian Amiri Alireza Iranbakhsh Mostafa Ebadi Halimeh Hassanpour Azade Hekmat
        AbstractMicrogravity is one of the environmental stresses in space that affects on the structure of cellular organelles and metabolites in plant. In the present research, the effect of microgravity in times 3, 7 and 10 days on root and hypocotyl growth rate, fresh and d More
        AbstractMicrogravity is one of the environmental stresses in space that affects on the structure of cellular organelles and metabolites in plant. In the present research, the effect of microgravity in times 3, 7 and 10 days on root and hypocotyl growth rate, fresh and dry weight, cell and organelles ultrastructure with Transmission Electron microscope (TEM) and some antioxidative capacity ( phenol, flavonoid and inhibition of DPPH radicals) were investigated in henbane (Hyoscyamus niger L.) seedling. The results showed that microgravity led to increase root and hypocotyle length as compared to control. Amydons were spread throughout the cell in microgravity treatment, while in control samples, they were deposited on the cell wall in the direction of gravity. Mitochondria were variable in shape and number. The endoplasmic reticulum in 10-day treatment was more voluminous and longer than the control. Also, the vacuole was observed more voluminous as compared to control. Total phenol, flavonoid, and inhibition of DPPH radicals increased significantly(P < 0.05). It seems, cellular changes and secondary metabolism induced under microgravity are related to oxidative stress. Manuscript profile
      • Open Access Article

        2 - Synergic effects of scopoletine and green synthesized silver nanoparticles on Angiogenesis of Chick Chorioallantoic Membrane (CAM)
        Parisa zamani esmati Javad Baharara Alireza Iranbakhsh Tayebeh Ramezani
        Background: Today, anti-angiogenesis properties of plants products are considered by researchers. Since the silver nanoparticles (AgNPs) are green synthesized and scopoletine, have anti-angiogenesis properties. In the present study, the use of these two substances has b More
        Background: Today, anti-angiogenesis properties of plants products are considered by researchers. Since the silver nanoparticles (AgNPs) are green synthesized and scopoletine, have anti-angiogenesis properties. In the present study, the use of these two substances has been investigated on the angiogenesis in the Chick Chorioalantoic Membrane. Materials and Methods: In this experimental study, the fertilized eggs were divided, the control and experimental groups including silver nanoparticles treated with nanoparticles (50, 100 and 150 μg/ml), scopoletine (25, 50 and 100 μg/ml), synergistic group, 50μg ml silver nanoparticles and 25 μg/ml scopoletin. In 8th day incubation, treatment was done on gelatin sponge and in 12th day were photographed and the number and lengths of vessels around the sponges, crown rump (CR), fetal weight and blood vessels hemoglobin levels were recorded and compared with control. Data were analyzed by SPSS-16 software, one way ANOVA and Tukey test in significant level of P <0.05. Results: The results showed that dose dependent manner of silver nano particles and scopolotine reduced the number and length of blood vessels and decreased level of hemoglobin concentration in treatment groups. The use of Synergic 50 μg/ml nanoparticles and 25 μg/ml concentration of scopoletine, which did not affect on the angiogenesis process, showed a significant decrease in angiogenesis (P<0.05). Conclusion: The use of green synthesized silver nanoparticles and scopolinoth has been shown to enhance their anti-angiogenic effects and can be considered as an important study basis to identify effective therapeutic strategis. Manuscript profile
      • Open Access Article

        3 - Study effect of mycorrhiza Glomus mosseae and brassinosteroid the mechanism of photosynthesis of anise (Pimpinella anisum L.) under cadmium stress
        sepideh hajbagheri Hosein Abbaspour Shekoofeh Enteshari Alireza Iranbakhsh
        Heavy metals are important environmental pollutants because of their toxicity, ecological, evolutionary, environmental, nutritional, and is considered as a major problem. Many studies have shown that plants inoculated with mycorrhizal fungi and the use of hormone Brassi More
        Heavy metals are important environmental pollutants because of their toxicity, ecological, evolutionary, environmental, nutritional, and is considered as a major problem. Many studies have shown that plants inoculated with mycorrhizal fungi and the use of hormone Brassinosteroids increased resistance of plants to heavy metals. In this study, the effect of mycorrhiza Glomus mosseae and 24-epibrassinolid (10-6 µM) on anise resistance to the stress of cadmium chloride (0, 100, 200 and 800 ppm) were compared. The results showed that cadmium reduced percentage of root mycorrhizal colonization, chlorophyll a, chlorophyll b and total chlorophyll, chlorophyll biosynthesis pathway intermediates containing protoporphyrin IX, magnesium protoporphyrin IX, Protochlorophilid, chlorophilid a and chlorophilid b and carotenoids in the anise plant. Plants pretreatment with brassinosteroid, plants inoculated with mycorrhizal fungi Glomus mosseae and interaction brassinosteroid and Glomus mosseae increased the amount of the compounds of cadmium chloride concentrations were 100 and 200 ppm. Therefore it can be concluded that Brassinosteroids and mycorrhizal fungi in the this concentrations on plant resistance and mechanisms of photosynthesis have a positive role and plant resistance to cadmium toxicity in this plant. Manuscript profile
      • Open Access Article

        4 - The effects of different concentrations of CaCL2 and MgSO4 on histological-cellular variation of microtubers in Solanum tuberosum In vitro conditions
        zahra zare Alireza Iranbakhsh Mostafa Ebadi
        The potato (Solanum tuberosum L.) is one of the most important agricultural plants in the world. It is propagated predominantly by asexual methods. The traditional methods for asexual propagation of the plant faced important problems. Therefore, the seed tubers can be r More
        The potato (Solanum tuberosum L.) is one of the most important agricultural plants in the world. It is propagated predominantly by asexual methods. The traditional methods for asexual propagation of the plant faced important problems. Therefore, the seed tubers can be replaced by micro tubers produced by tissue culture. The aim of this study is search about effect of different concentrations of CaCL2 and MgSO4 in media culture In vitro in histological - cellular variations of the microtubers. In this study solid and liquid MS media were used to prepare sterilized seedlings and micro tuberization.The concentrations of 0, 1/2, 1, 3/2 and 2 times more than standard concentrations of the mentioned compounds in MS medium were used in separate induction media . Induction was done in the alternating photoperiod. In order to the developmental and anatomical studies cross sections of microtubers was prepared and studied by light microscopy.The number of cell rows, the dimensions of the cells and the starch content of the parenchymal tissues of microtuber were analyzed. the results showed significant variations in histological features of the microtubers developed in media containing different concentrations of macronutrients. CaCL2 with concentrations of 0.5 to 1 time more than standard concentration in MS medium yielded maximum number of cell rows and maximum starch granules content. However, the presence of a minimum amount of magnesium is essential for the formation of the micro tubers. But there is no statistically significant effect on cellular changes of microtubers and starch content. Manuscript profile
      • Open Access Article

        5 - The effect of Selenium nanoparticles on some morphological and anatomical features of cherry tomato plants (Solanum lycopersicum L.var. cerasiforme).
        maryam neysanian علیرضا ایرانبخش Rahim Ahmadvand zahra Oraghi Ardebili Mostafa Ebadi
        Selenium is a beneficial element with antioxidant properties that increases plant growth and tolerance to abiotic stresses. In order to investigate the effect of Nano Selenium solution treatments on Cherry Tomato plant growth, treatments including Sodium Selenate (bulk) More
        Selenium is a beneficial element with antioxidant properties that increases plant growth and tolerance to abiotic stresses. In order to investigate the effect of Nano Selenium solution treatments on Cherry Tomato plant growth, treatments including Sodium Selenate (bulk) and Selenium nanoparticles were applied at four levels (0 as control, 2, 4 and 10 ppm). The experimental results showed that interaction between Selenium nanoparticles and all studied traits (fresh weight, dry weight, anatomical indices of roots and stems and leaves, chlorophyll content) were significantly different at 1% level. Treatments caused morphological changes in stems, roots and leaves compared to controls. The highest increase in root and leaf diameter was observed in group four Nano Selenium (21.55 and 1.1%) compared to the control. All treatments reduced stem diameter compared to control. An increasing trend of root and stem central cylinder thickness was observed in proportion to the concentration compared to the control. Both Nano-Selenium and bulk Selenate reduced the differentiation of xylem vessels in leaves compared to the controls. The results of studied morphological and anatomical traits indicated that the use of two levels of Nano-Selenium concentrations, 2 and 4 ppm, improved the growth parameters, in contrast, application of 10 ppm Nano-Selenium concentration reduced the growth parameter, the content of plant pigments in comparison with the control sample. Overally, the results showed that Selenium treatment in appropriate amounts can contribute to the optimal yield of cherry tomatoes and increase plant growth and pigmentation and affect tissue differentiation and organ development. Manuscript profile