The effects of different concentrations of CaCL2 and MgSO4 on histological-cellular variation of microtubers in Solanum tuberosum In vitro conditions

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

zahra zare ¹ , Alireza Iranbakhsh ² , Mostafa Ebadi ³

1 - Department of Biology, Farhangian university, Tehran, Iran.
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Biology, Damghan Branch, Islamic Azad University, Semnan, Iran.

Received: 2018-06-28 Accepted : 2018-09-22 Published : 2018-12-11

Keywords: Microtuberization, Invitro, CaCl2, Histological-Cellular variation, MgSO4,

Abstract :

The potato (Solanum tuberosum L.) is one of the most important agricultural plants in the world. It is propagated predominantly by asexual methods. The traditional methods for asexual propagation of the plant faced important problems. Therefore, the seed tubers can be replaced by micro tubers produced by tissue culture. The aim of this study is search about effect of different concentrations of CaCL2 and MgSO4 in media culture In vitro in histological - cellular variations of the microtubers. In this study solid and liquid MS media were used to prepare sterilized seedlings and micro tuberization.The concentrations of 0, 1/2, 1, 3/2 and 2 times more than standard concentrations of the mentioned compounds in MS medium were used in separate induction media . Induction was done in the alternating photoperiod. In order to the developmental and anatomical studies cross sections of microtubers was prepared and studied by light microscopy.The number of cell rows, the dimensions of the cells and the starch content of the parenchymal tissues of microtuber were analyzed. the results showed significant variations in histological features of the microtubers developed in media containing different concentrations of macronutrients. CaCL2 with concentrations of 0.5 to 1 time more than standard concentration in MS medium yielded maximum number of cell rows and maximum starch granules content. However, the presence of a minimum amount of magnesium is essential for the formation of the micro tubers. But there is no statistically significant effect on cellular changes of microtubers and starch content.

References:

[1]     احمدی، س و جبارزاده، ز. 1395، تاثیر غلظت‌های مختلف جیبرلیک اسید و بنزیل آمینو پورین در ریز ازدیادی زیتون دو رقم کرونایکی و دزفولی، اولین همایش ملی کشت سلول و بافت گیاهی، اصفهان، دانشگاه آزاد اسلامی واحد اصفهان (خوراسگان).

[2]     احمدیان، ش؛ عبدمیشانی، س و ضرغامی، ر. 1374. تولید ریزغده­های عاری از ویروس سیب زمینی از طریق کشت بافت. پایان نامه کارشناسی ارشد،دانشگاه آزاد اسلامی واحد کرج.

[3]     بلندی، ا؛ حفیظی، ب و حمیدی، ح. 1395. تأثیر ساکارز و هورمون بر ریزغده­زایی سه رقم سیب زمینی در کشت درون شیشه‌ای. فناوری زیستی در کشاورزی. 15(2): 59- 67.

[4]     رشیدی، ط. 1383. بررسی تراکم‌های مختلف کاشت گیاهچه‌های حاصل از کشت بافت بر آنالیزهای رشد و تولید مینی تیوبر در دو رقم سیب زمینی. پایان‌نامه کارشناسی ارشد، دانشگاه آزاد اسلامی واحد ورامین.

[5]     روستا، ح؛ وزیری نسب، س و رقامی، م 1394. اثر 6- بنزیل آمینوپورین و سایکوسل بر تولید ریزغده در دو رقم سیب زمینی در شرایط درون شیشه ای. علوم باغبانی ایران. 46(1): 141- 156.

[6]     عبادی، م. 1380 بررسی روند تکوینی سلولی گیاه سیب‌زمینی Solanum tuberosum در کشت بافت، سلول و بیوراکتورهای نیمه پیوسته و پیوسته پایان نامه دوره‌ی دکتری، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران.

[7]     قربانلی، م. 1372 فیزیولوژی گیاهی. تهران: مرکز نشر دانشگاهی: 97-100.

[8]     کشمیری، ا؛ کافی، م؛ پارسا، م؛ نباتی، ج و زارع مهرجردی، م. 1397. اثر سطوح مختلف کود نیتروژن و اسیدیته محلول غذایی بر ویژگی‌های فیزیولوژیک و تولید ریزغده سیب‌زمینیSolanum tuberosum L. فیزیولوژی گیاهان زراعی. 10(37): 97-118.

[9]     مجد، ا و شریعت زاده، م. 1377. زیست‌شناسی سلولی. انتشارات اراک: 379-373.

[10] Ashrafzadeh, S. and Leung, D.W.M. 2015. Microtuber formation in potato callus. ScienceAsia,41(1):1-4.

[11] Ebadi, M. and Iranbakhsh, A. 2011. The induction and growth of potato (Solanum tuberosum. L) microtubers (sante cultivar) in response to the different concentrations of 6-benzylaminopurine and sucrose. African Journal of Biotechnology,10(52): 10626-10635

[12] Fufa, M. and Diro, M. 2013.The Effects of Sucrose on in vitro Tuberization of Potato Cultivars.  Advances in Crop Science and Technology,1(4): 1-3.

[13] Gami R. A., Parmar S. K., Patel P. T., Tank C. J., Chauhan R. M., Bhadauria H.S. and Solanki S.D. 2013. Microtuberization, minitubers formation and in vitro shoot regeneration from bud sprout of potato (Solanum tuberosum L.) cultivar K. Badshah. African Journal of Biotechnology, 12 (38): 5640-5647.

[14] Gopal, J., Minocha, J. L, Dhaliwal, H. S. 1998. Microtuberization in potato (Solanum tuberosum.L). Plant Cell Reports (1998) 17: 794–798.

[15] Higgins, Sh., Francis, R. D. and Smith, A. M., 2017. Production of Solanum tuberosum L. Microtuber Using Temporary Immersion System. European Journal of Experimental Biology. 7(6): 1- 3.  

[16] Hoque, M. E. 2010. In vitro tuberization in potato (Solanum tuberosum L.). Plant Omics Journals, 3 (1):7-11.

[17] Husain, S., Shah, S. A. H., Asghar, S., Hussain, N., Ali, N, Hussain, I., Rafiq, S., Shah, S., Imtiaz, M., Ali, M., Jala, F., and Rashid, M. 2017. Micro-Tuberization of Four Potato (Solanum tuberosum. L) Cultivars Through Tissue Culture. Journal of Botanical Sciences. 6 (3): 35-40.

[18] Jao, R.C and Fang, w. 2004. Growth of potato plantlets in vitro is different when provided concurrent versus alternating Blue and Redlight photoperiods. Hortscience, 39(2):380-382.

[19] Joshi, A. and Mature, N., 2015. Micropropagation and Conservation of Endanger Medicinal Plant – Leptadenia rticulata (Retz.) wight and Arn. Trought Nodal Explant. International Journal of Current Advanced Research. 4(9): 382-385.

[20] Khalil, M.M., Abd El Aal, A. M. H.  and Samy, M. M. 2017. Studies on Microtuberization of Five Potato Genotypes. Egyptian Journal of Horticulture, 44 (1): 91- 97.

[21] Pevalek – Kozlina, B. and Berljack, J. 1997. Starch accumulation as a marker For Microtuberization in potato (Solanum tuberosum L.) Biologia – section Botany, 52 (4): 553-559.

[22] Reeve, RM., Timm, H. and Weaver, M.L. 1973. Parenchyma cell growth in potato tubers I. Different tuber regions. American potato journal, 50 (2): 49-57.

[23] Seabrook, JEA., Douglass, L.K, and Arnold, DA. 2004. Effect of leaves on microtubers produced from potato single -  node cuttings invitro .American  Journal of Potato Research, 81 (1): 1-5.

[24] Sonnewald, S. and Sonnewald, U. 2014. Regulation of potato tuber sprouting. Planta, 239:27–38.

[25] Tabori, K.M., Dobranszki, J. and Ferenczy, A. 2000. Efeects of culture density on growth and in vitro tuberization capacity of potato plantlets. Acta - Agronomica - Hungaria. 48 (2): 185 – 189.

[26] Westermann, D.T. 2005. Nutritional Requirements of potato. American Journal of potato Research, 82:301-307.

[27] Xu, X., Vreugdenhil, D. and Lammeren, A.A.M. 1998. Cell division and cell enlargement during potato tuber formation. Journal of Experimental Botany, 49(320): 573- 582.