Developmental Biology: 1 (2)

Open Access Article

1 - Molecular identification and study of water Frogs phylogeny in South eastern Tehran province using mitochondrial 12srRNA gene Sequence
Siamak Yousefi Siahkalroodi Farideh Chenari Mina Babaei Mahyar Yousefi Siahkalroodi

Up to now most species of Iranian frogs has been identified morphologically and all populations (albeit their vast distribution) are assigned to Rana (Pelophylax) ridibunda. The main objective of this research was to study frog populations of the South eastern Tehran us More

Up to now most species of Iranian frogs has been identified morphologically and all populations (albeit their vast distribution) are assigned to Rana (Pelophylax) ridibunda. The main objective of this research was to study frog populations of the South eastern Tehran using mitochondrial 12srRNA sequencing. For this purpose, n=19 water frogs were sampled in four stations (i.e. Pakdasht, Varamin, Gharchak, Pishva). The samples specimens were preserved at 96% ethanol solution and transferred to the laboratory for further analysis. DNA was extracted from leg tissue using CTAB method and the 12srRNA gene was ampilified and sequenced using primers developed by Kocher et al. (1989). The phylogenic tree was drawn using Joining Neighbor and Maximum Likelihood methods in Mega6 software the results indicate that indicated two clads with 100% affinity. The first clade assigned to P. bedriagae populations and the next clade included our samples. In conclusion, the frog populations of Tehran province may be assigned to P. bedriagae or a subspecies. Further research is needed to reveal this. Manuscript profile

Open Access Article

2 - Study the effect of biological and chemical fertilizers on Artemisia annua L. quantitative characteristics after and before flowering
Mohammad Javad Shakouri Mohammad Hossein Bijeh Keshavarzi

Artemisia annua L. is one of the important herbal plants, which is applicable for some disease treatment such as, Cancer and Malaria. To consider the effect of biological and chemical fertilizers on Artemisia annua L. quantitative characteristics an experiment was carri More

Artemisia annua L. is one of the important herbal plants, which is applicable for some disease treatment such as, Cancer and Malaria. To consider the effect of biological and chemical fertilizers on Artemisia annua L. quantitative characteristics an experiment was carried out in factorial design in completely randomized design with 4 replications in a educational green house in Tehran. Treatments included chemical fertilizers (Nitrogen (N), Phosphorus (P)) in 4 levels (N0P0, N40P40, N80P40, N80P80) and biological fertilizers in 4 levels (control, Nitroxin, Bio-phosphorus and Vemricompost fertilizer. Results showed that using biological fertilizer, and increasing different levels of chemical fertilizers (N, P) had significant effect on under consideration characteristics. Means comparison showed that biological fertilizers application leads to significant increase in all under consideration features. Among biological fertilizer, Vemricompost treatments the most effective. Means comparison of applying different levels of chemical fertilizers indicated that N80P80 had the most increase in features. Interaction effect had different effects on those features. Manuscript profile

Open Access Article

3 - Investigation the effect of carboplatin on oogenesis and uterus changes in adult female NMRI mice strain
Sepideh Gholami Yarahmadi nasim HAYATI ROODBARI KAZEM PARIVAR Gholamhassan Vaezi

Cancer is one of the most common diseases around the world. The most important therapies for cancer are chemotherapy that is performed by various medications. One of the common drugs in chemotherapy is “Carboplatin”. In this study, the effects of Carboplatin More

Cancer is one of the most common diseases around the world. The most important therapies for cancer are chemotherapy that is performed by various medications. One of the common drugs in chemotherapy is “Carboplatin”. In this study, the effects of Carboplatin on oogenesis and uterine changes in NMRI mouse strain have been investigated In vivo. In this study, 30 adult female mice were randomly divided into five groups: control, sham and three experimental groups. Experimental groups were injected Carboplatin with 0.36, 0.18, 0.09 mg/ml density for 28 days and all were injected subcutaneously. After describing the animals, the ovary and uterus were separated and subjected to histopathologic and stained by hematoxylin and eosin. Blood sampling was performed to study both estradiol and FSH. The results were analyzed using one-way ANOVA and SPSS software. By using Carboplatin,, the number of primordial, secondary, Graafian follicles, corpus lutea and blood vessels decreased and the number of atretic follicles increased. The diameter of ovary in experimental groups decreased, but there was no significant change in diameters of follicles. Uterine studies showed that, the uterine’ diameter and layers, and the diameter of lumen reduced. In addition, the number of uterine glands decreased. Also, the levels of FSH and estradiol decreased. Regarding to using Carboplatin, this drug by decrease in the number of primordial, secondary and Graafian follicles, increase in number of atretic follicles, and also disruption of the hormonal system, the probability of premature aging may increase and has a negative effect on fertility. Manuscript profile

Open Access Article

4 - New notes on Acer L. species in Iran
fatemeh asadi Fariba Sharifnia fahimeh salimpoor ahmad majd

The genus Acer belongs to the family Sapindaceae, about 156-124 species. Acer, as one of the largest genera of the Sapindaceae, is distributed in Asia, Europe, North America and North Africa. It has 16 sections in recent categories. Eight species have been mentioned in More

The genus Acer belongs to the family Sapindaceae, about 156-124 species. Acer, as one of the largest genera of the Sapindaceae, is distributed in Asia, Europe, North America and North Africa. It has 16 sections in recent categories. Eight species have been mentioned in different sources for Iran and Alborz and Zagros Mountains are the main centers of distribution of this species in Iran. The most important characteristic of this genus is that it has two winged nuts or bisamara as well as its opposite leaves. In line with the phylogenetic revision of the species, A. gilanense is introduced to the world for the first time. In this study, due to the close affinity of the new species with A. cappadocicum var. stenocarpum, compared with it. Investigations have been carried out using morphological features, intergenic spacer regions (ITS), and micro-morphological study of Samar fruit. A new identification key for Acer species is also presented in Iran. Manuscript profile

Open Access Article

5 - Quality and quantity Comparison of clustered Sa oregano essence(Nepeta racemose ) at different stages of growth
fateme zandi toghan giti barzin ali mazooji

Clustered Oregano Sai with the scientific name, Nepeta racemosa L. from Spearmint family, is a plant like a grass, and one of the most important medicinal plants in the world and our country that has significant amounts of secondary metabolites and is especially essence More

Clustered Oregano Sai with the scientific name, Nepeta racemosa L. from Spearmint family, is a plant like a grass, and one of the most important medicinal plants in the world and our country that has significant amounts of secondary metabolites and is especially essence. Useful material of this plant, is using in the medicine field and pharmaceutical industry and cosmetics and hygienic field.(khalad Barin and Islam zade, 1380). The purpose of this study is to analyze and study the essence oil of clustered Sai Oregano plant at different growth stages. clustered Sa oregano essence plant (Napata racemosa) from mid-July to early of August 1393 during phase before flowering, After flowering and fruiting phase in Haraz Road area after the Holy Hashem ( imam zade) Plur three ways toward Lar, is collected After identification. After collecting and drying, essence oils of samples are taken by using distillation and Clevenger apparatus. Clustered Sa oregano essence, monoterpene component has highest percentage of plant clustered Sa oregano essence, in all 5 stages. In the meantime, the two combined composition of Nptalactones and 1,8-cineole were higher than the other compounds. In many of the studies on the chemical composition of essences that are exist in the resources; Monoterpenes compounds constitute the most of their component (33). And in some of them Npta monoterpene lactones and 1,8-cineole are main ingredients of essence. Manuscript profile

Open Access Article

6 - Investigate of miR-21 expression level in urine samples of people with prostate cancer
Alireza Emamvirdizadeh mehrdad hashemi franak jamshidian

Introduction: Prostate cancer is the most common cancer in men A group of diagnostic biomarkers is MicroRNA, they can be used to diagnose variable diseases, especially cancers. this study, we investigate changes in the expression level of miR-21 in urine, in people with More

Introduction: Prostate cancer is the most common cancer in men A group of diagnostic biomarkers is MicroRNA, they can be used to diagnose variable diseases, especially cancers. this study, we investigate changes in the expression level of miR-21 in urine, in people with prostate cancer and healthy group. Materials and methods: This research was done on 70 urine samples of prostate cancer patients (32 Metastatic and 38 Non-metastatic) and 30 control samples with negative biopsy report. First RNA was extracted with Trizol, after the cDNA synthesis, changes in the expression of miR-21 and miR-214 in the urine of people with prostate cancer and healthy group were investigated by using Real-time PCR. Statistical analysis of data was calculated with REST 2009. Results: The results revealed the presence of miR-21 in urine samples in patients with prostate cancer, and it was found that miR-21 showed a significant increase in expression (P=0.003) than healthy group. miR-21 in metastatic group (P=0.042) demonstrate over expression than non-metastatic group (P=0.036). Conclusions: The results show that, miR-21 has significant change in expression level in patients with prostate cancer in compare of healthy group, and can be a non-invasive method to detect prostatic patients and also use this biomarker to determine metastatic and non-metastatic group. Manuscript profile

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