Investigate of miR-21 expression level in urine samples of people with prostate cancer

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Alireza Emamvirdizadeh ¹ , mehrdad hashemi ² , franak jamshidian ³

1 - Faculty of Advanced Scienced and technology, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran
2 - Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
3 -

Received: 2019-04-17 Accepted : 2019-12-02 Published : 2020-05-21

Keywords: urine, biomarker, prostate cancer, miR-21,

Abstract :

Introduction: Prostate cancer is the most common cancer in men A group of diagnostic biomarkers is MicroRNA, they can be used to diagnose variable diseases, especially cancers. this study, we investigate changes in the expression level of miR-21 in urine, in people with prostate cancer and healthy group. Materials and methods: This research was done on 70 urine samples of prostate cancer patients (32 Metastatic and 38 Non-metastatic) and 30 control samples with negative biopsy report. First RNA was extracted with Trizol, after the cDNA synthesis, changes in the expression of miR-21 and miR-214 in the urine of people with prostate cancer and healthy group were investigated by using Real-time PCR. Statistical analysis of data was calculated with REST 2009. Results: The results revealed the presence of miR-21 in urine samples in patients with prostate cancer, and it was found that miR-21 showed a significant increase in expression (P=0.003) than healthy group. miR-21 in metastatic group (P=0.042) demonstrate over expression than non-metastatic group (P=0.036). Conclusions: The results show that, miR-21 has significant change in expression level in patients with prostate cancer in compare of healthy group, and can be a non-invasive method to detect prostatic patients and also use this biomarker to determine metastatic and non-metastatic group.