About the journal
Recent Articles
-
Open Access Article
1 - Behavioral and electrophysiological study on bone marrow stromal cell transplantation with olive extract in repair of severed sciatic nerve in rats
MOSTAFA MOAZAMI GODARZI Nasim Hayati Roodbari Gholamreza Kaka Kazem ParivarIssue 2 , Vol. 16 , Spring 2024Background and purpose: Behavioral and electrophysiological study on the use of bone marrow stromal cell transplantation along with olive extract in the repair of severed sciatic nerve in rats.Materials and Methods: After sciatic nerve amputation, adult male rats were r MoreBackground and purpose: Behavioral and electrophysiological study on the use of bone marrow stromal cell transplantation along with olive extract in the repair of severed sciatic nerve in rats.Materials and Methods: After sciatic nerve amputation, adult male rats were randomly divided into 4 groups of 7.Healthy rats, rats with sciatica without treatment intervention, rats with bone marrow stromal cells treated with olive extract at the site of amputation, rats with bone marrow stromal cells injected at the site of injury.The rate of recovery was assessed by sensory motor activity of the sciatic nerve, electrophysiological studies.Results: Sciatic nerve motor evaluation, no control group returned to normal in the eighth week, cell therapy group was restored with olive extract in the eighth week. The level of AMP in the eighth week after the restoration of the cell therapy group with a gentle slope indicates the recovery process of the cell therapy group.Counting the number of nerve fibers at an area of 1000 μm, the number of nerve fibers in the cell therapy groups increased in the eighth week after repair, compared with the control group and the PLGA membrane group. By the end of the eighth week, the sciatic nerve index (Hot Plate test), the healing process of the cell therapy group with olive extract was more evident to other groups.Conclusion: bone marrow stromal cell transplantation repairs sciatic nerve and olive extract along with bone marrow stromal cell accelerates sciatic nerve repair. Manuscript profile -
Open Access Article
2 - Evaluation of miR-192 expression in lymph nodes of sarcoidosis patients by Real-time PCR method and comparison of their expression with the control group
Samaneh sadat Rasi Varaei Abdolreza Mohamadnia Monireh Movahedi naghmeh Bahrami Sadegh ShirianIssue 2 , Vol. 16 , Spring 2024Sarcoidosis is a disease with an unknown cause and the onset of the disease is often gradual. The disease may be asymptomatic or chronic. Examining the presence of factors such as micro RNAs may lead to better diagnoses of sarcoidosis and help to better understand the m MoreSarcoidosis is a disease with an unknown cause and the onset of the disease is often gradual. The disease may be asymptomatic or chronic. Examining the presence of factors such as micro RNAs may lead to better diagnoses of sarcoidosis and help to better understand the mechanism of sarcoidosis.This is a case-witness study that after obtaining permission from the ethics committee, 40 tissue samples from the lymph nodes of patients with sarcoidosis and 40 control samples were collected with the opinion of an expert doctor, and then RNA was extracted from the lymph nodes. Realtime PCR was done for miR-192 marker.miR-192 biomarker was positive in the lymph node samples of sarcoidosis patients in 28 out of 40 people. The positive rate of this biomarker in healthy people was 17 out of 40 people. miR-192 biomarker showed increased expression in sarcoidosis patients compared to healthy samples.According to the results of this study, miR-192 may be used as a molecular marker for sarcoidosis. It also has significant potential for diagnosis, prognosis and even treatment of sarcoidosis. Manuscript profile -
Open Access Article
3 - Investigation of hydroalcoholic extract effect of Cordia Myxa fruit on the expression of BCL-2 and BAX genes in the apoptotic pathway and cell proliferation in human lung cancer cell line.
NAHID ASKARI Kian Aghaabbasi elham rezvannejadIssue 2 , Vol. 16 , Spring 2024Cancer is one of the most important causes of death worldwide. On the other hand, the therapeutic use of medicinal plants has increased during recent years. For this purpose, the aim of the present research was to investigate the effect of a Cordia myxa fruit extract on MoreCancer is one of the most important causes of death worldwide. On the other hand, the therapeutic use of medicinal plants has increased during recent years. For this purpose, the aim of the present research was to investigate the effect of a Cordia myxa fruit extract on cellular proliferation and apoptosis gene expression (BCL-2 and BAX) in lung cancer cell line (A549). The plant was collected and Hydroalcoholic extract was prepared by macerating method. Various concentrations of Cordia myxa extracts (of 0.25, 0.5, 1, 2, 4 mg/mL) obtained to treat lung cancer and fibroblast cell lines. Cells were examined by MTT-assay and Real Time RT-qPCR to determine the plant extract-induced cytotoxicity in cell lines with and without treatment The results showed that, the concentration of ≥2 mg/ml extract had a significant lethal effect on cancer cells. The expression of BAX gene in the treated cell line increased and the expression of BCL-2 gene decreased compared to the control. The extract of Cordia myxa fruit didn’t show toxicity effects when used in low concentrations. However, this extract demonstrated the lower cytotoxicity against normal fibroblast cells. The combination therapy can be less toxic if both (plant extract and chemotherapeutic agent) use to treat cancers. It can be useful with fewer side effects of chemotherapeutic agents. Manuscript profile -
Open Access Article
4 - Effects of Chaste tree fruit extract on ovarian tissue and sex hormones of POF model mice
kiana ghorbani Seyedeh Fatemeh Siadat Rohollah Fathi Fatemeh Abedi GhehiIssue 2 , Vol. 16 , Spring 2024Premature ovarian failure (POF) is known as a heterogeneous disorder that leads to amenorrhea and ovarian failure before the age of 40 and causes infertility in young women. Herbal Medicines is traditionally used to treat hormonal problems in women. This study was condu MorePremature ovarian failure (POF) is known as a heterogeneous disorder that leads to amenorrhea and ovarian failure before the age of 40 and causes infertility in young women. Herbal Medicines is traditionally used to treat hormonal problems in women. This study was conducted to investigate the effect of Chaste tree fruit hydro alcoholic extract on ovarian function and body weight of mice after POF induction in mice. In this study, 50 mice were divided into five groups (n=10) including the vitex group, POF and three experimental groups (creating POF model and gavage of vitex extract). After the effect of the extract, the ovarian tissue was studied with hematoxylin-eosin staining, and follicular count and serum level were checked. The results showed that the number of follicles, corpus luteum, and body weight of mice in the POF group were significantly reduced. Also, the serum level of FSH hormones increased in the POF group and the E2 hormone decreased significantly compared to the control group (p<0.05), which indicates the correct creation of POF models. After gavage of extract in POF mouse models, the serum level of hormones was adjusted and the number of ovarian follicles increased. The results of the study showed that vitex extract in the highest tested dose has positive effects on the number of follicles, body weight and sex hormones of mice in premature ovarian failure syndrome. Manuscript profile -
Open Access Article
5 - Investigating the correlation and cause and effect relationships of leaf yield and some important agronomic traits in basil genotypes under different drought stress conditions
Mehdi Rahimi Mojtaba MortazaviIssue 2 , Vol. 16 , Spring 2024Basil is one of the important plants belonging to the mint family, which is used as a medicinal plant, spice, and also as a fresh vegetable. In order to study the relationship between leaf yield and agricultural traits, 22 basil genotypes were evaluated on the basis of MoreBasil is one of the important plants belonging to the mint family, which is used as a medicinal plant, spice, and also as a fresh vegetable. In order to study the relationship between leaf yield and agricultural traits, 22 basil genotypes were evaluated on the basis of split plot design based on randomized complete block design with three replications in pots under field conditions. The main plot included drought stress in three levels (normal, moderate and severe stress) and the subplot included genotype (22 levels) and agricultural traits were measured. Correlation of traits in all three conditions showed that leaf yield had a positive and significant correlation with the traits of stem fresh weight, number of leaves, length and width of leaves. Considering the leaf yield as a dependent variable and performing a stepwise regression analysis in normal conditions (stem wet weight, number of leaves and leaf width in the first stage, respectively), moderate drought stress conditions (stem dry weight in the first stage) ) and severe drought stress conditions (stem wet weight in the first stage) were entered into the model and the model was significant at the level of 1%. Path analysis showed that stem wet weight in normal conditions, stem dry weight in moderate stress condition and stem wet weight in severe stress condition had the highest direct and positive effect on leaf yield. Therefore, the most important traits as a selection index for improving leaf yield werestem fresh weight, leaf number, leaf length and width, respectively. Manuscript profile -
Open Access Article
6 - Anatomical structure study of vegetative organs of Cordia myxa L. (Sepestan) in three south-Iranian habitat.
Maryam nikkhah Sedigheh Arbabian Ahmad Majd Fariba SharifniaIssue 2 , Vol. 16 , Spring 2024The Cordia myxa L. belongs to the borage family, which is very important in traditional medicine and wood industry. It is a medicinal plant with antibacterial, anti-inflammatory, anti-allergic, anti-viral and anti-cancer properties. Also, as a very fast-growing tree spe MoreThe Cordia myxa L. belongs to the borage family, which is very important in traditional medicine and wood industry. It is a medicinal plant with antibacterial, anti-inflammatory, anti-allergic, anti-viral and anti-cancer properties. Also, as a very fast-growing tree species in the southern provinces of the country, it can be used in artificial forests, landscaping and industry. Since this species has a good distribution in different habitats of southern Iran, this study was conducted to compare and investigate shoot anatomical features of three different parts of this plant in different habitats. Anatomical-developmental characteristics of three different habitats. Examination of the anatomical structure of vegetative organs showed differences in the thickness of the collenchyma area of stem, the size and cohesion of the cells, differences in the thickness of spongy and ladder mesophyll, and also the size of the brain parenchyma. Given to anatomical structure of the petiole, thickness of cortex, v Manuscript profile -
Open Access Article
7 - Detection of transgenic soybeans through Nos terminator and Roundup ready genes in baby food samples
Mahsa Kavousi Fatemeh TayefekhaniIssue 2 , Vol. 16 , Spring 2024A large amount of soy consumed in Iran is imported and transgenic, but there is no system for labeling transgenic products, even baby food. In this study, PCR assay of NOS Terminator primer gene and Roundup Ready transgenic marker gene was used to identify Randup-resist MoreA large amount of soy consumed in Iran is imported and transgenic, but there is no system for labeling transgenic products, even baby food. In this study, PCR assay of NOS Terminator primer gene and Roundup Ready transgenic marker gene was used to identify Randup-resistant transgenic soybean in baby food. 25 samples of soy and 25 samples of baby food were collected randomly. After DNA extraction with TLAB brand tissue extraction kit and DNA concentration and quality, PCR was performed. The results showed that with 95% confidence we can say that there is no significant relationship between the presence of NOS Terminator in soy and baby food. While with 95% confidence we can say that there is a significant relationship between the presence of Roundup Ready in soy and baby food. The amount of transgenic soy used in baby food is less than transgenic soy studied in this study. It can be concluded that the rate of transgenics in the tested samples is significantly higher. None of these products had a transgenic label. Manuscript profile -
Open Access Article
8 - Evaluation of Eugenol effect, active compound of clove oil, on growth inhibition of methicillin resistant isolates of Staphylococcus aureus through potential interaction with PBP2 and downregulation of mecA gene
Nahid Khodadadi Somayeh Ataei-e JalisehIssue 2 , Vol. 16 , Spring 2024Background and Objectives: Eugenol is the major constituents of clove oil, which is known as an anti-bacterial and anti-oxidant agent. Staphylococcus aureus is most common pathogens that cause nosocomial infections and some bacterial infections death. In this study, the MoreBackground and Objectives: Eugenol is the major constituents of clove oil, which is known as an anti-bacterial and anti-oxidant agent. Staphylococcus aureus is most common pathogens that cause nosocomial infections and some bacterial infections death. In this study, the effect of Eugenol on the expression of mecA gene and the interaction with mecA protein (PBP2a) was evaluated in methicillin resistant isolates of Staphylococcus aureus. Materials and methods: Antibacterial activity of Eugenol was investigated in isolates of Staphylococcus aureus with DISC diffusion, MIC and MBC. Interaction between Eugenol and mecA protein (PBP2) was evaluated with bioinformatic approaches. The expression of mecA gene was assessed in Eugenol treated and untreated isolates by Q-RT-PCR. Results: results of this study showed that Eugenol inhibited bacterial growth in the DISCs in 80% of Staphylococcus aureus isolates. MIC and MBC of Eugenol in the most isolates were determined 5.22 mg/ml and 10.44 mg/ml, respectively. In silico analysis showed interaction between Eugenol and mecA protein (PBP2). mecA gene downregulated in Eugenol treated isolates (5.22 mg/ml) in compared to control. Conclusion: It seems that Eugenol led to growth inhibition of Staphylococcus aureus isolates through both interaction with PBP2 and mecA gene downregulation. Manuscript profile
Most Viewed Articles
-
Open Access Article
1 - Bioinformatics analysis of the chloroplast genome of G.stocksii and G.longicalyx diploid species and comparing them with tetraploid species
Farshid Talat Mahrokh GheibiIssue 1 , Vol. 15 , Winter 2023Cotton (Gossypium spp.) included 5 tetraploid (2n=4x=52) and more than 45 diploid (2n=2x=26) species. Tetraploid species of cotton are formed through hybridization between an A- genome and a D- genome. Genome mapping analyses showed that there is high level of similarit MoreCotton (Gossypium spp.) included 5 tetraploid (2n=4x=52) and more than 45 diploid (2n=2x=26) species. Tetraploid species of cotton are formed through hybridization between an A- genome and a D- genome. Genome mapping analyses showed that there is high level of similarity between diploid A- and D- genomes with tetraploid At- and Dt- genomes. G. stocksii and G.longicalyx belong to D genome, which is an important wild bio-source for the cotton breeding and genetic research. Present study was conducted to study and compare the complete chloroplast sequence of two diploid species of cotton (G.stocksii and G.longicalyx) compared with two tetraploid species (G.hirsutum and G.barbadense), analyses of their genome structure, gene content and organization, repeated sequences and codon usage has been carried out. G.longicalyx chloroplast (cp) genome is 160,248 bp in length which longer than G.stocksii and shorter than two tetraploid species. G.stocksii chloroplast (cp) genome is 159,039 bp in length which shorter than G.longicalyx and shorter than two tetraploid species. In both species single copy regions of cp genome is separated by the two inverted repeats. The plastidic genomes of G.stocksii and G.longicalyx comprised of 79 protein coding genes, 4 ribosomal RNA genes and 30 transfer RNA genes, 113 single genes in total and 20 duplicated genes in inverted repeats. In both diploid species 5 ycf genes with unknown function have been identified. These genes considered essential for plants and are highly conserved in plant taxa. Manuscript profile -
Open Access Article
2 - Effects of Chaste tree fruit extract on ovarian tissue and sex hormones of POF model mice
kiana ghorbani Seyedeh Fatemeh Siadat Rohollah Fathi Fatemeh Abedi GhehiIssue 2 , Vol. 16 , Spring 2024Premature ovarian failure (POF) is known as a heterogeneous disorder that leads to amenorrhea and ovarian failure before the age of 40 and causes infertility in young women. Herbal Medicines is traditionally used to treat hormonal problems in women. This study was condu MorePremature ovarian failure (POF) is known as a heterogeneous disorder that leads to amenorrhea and ovarian failure before the age of 40 and causes infertility in young women. Herbal Medicines is traditionally used to treat hormonal problems in women. This study was conducted to investigate the effect of Chaste tree fruit hydro alcoholic extract on ovarian function and body weight of mice after POF induction in mice. In this study, 50 mice were divided into five groups (n=10) including the vitex group, POF and three experimental groups (creating POF model and gavage of vitex extract). After the effect of the extract, the ovarian tissue was studied with hematoxylin-eosin staining, and follicular count and serum level were checked. The results showed that the number of follicles, corpus luteum, and body weight of mice in the POF group were significantly reduced. Also, the serum level of FSH hormones increased in the POF group and the E2 hormone decreased significantly compared to the control group (p<0.05), which indicates the correct creation of POF models. After gavage of extract in POF mouse models, the serum level of hormones was adjusted and the number of ovarian follicles increased. The results of the study showed that vitex extract in the highest tested dose has positive effects on the number of follicles, body weight and sex hormones of mice in premature ovarian failure syndrome. Manuscript profile -
Open Access Article
3 - Histological study of seed development in Tribulus terrestris L. (Zygophyllaceae)
fahimeh mokhtari elham mohajel kazemi mina kazemian mahboubeh aliasgharpour ali movafeghiIssue 2 , Vol. 15 , Spring 2023The study of embryonic and endosperm development in different plant species is important from a phylogenetic and systematic point of view. The aim of the present study was to investigate the histological evaluation of seed development in Tribulus terrestris L. by micros MoreThe study of embryonic and endosperm development in different plant species is important from a phylogenetic and systematic point of view. The aim of the present study was to investigate the histological evaluation of seed development in Tribulus terrestris L. by microscopic analysis. Samples were studied at different developmental stages after fixation by different histological techniques. The results showed that the ovules were anatropous, bilayer and axially placentation. Endosperm was observed in the early stages, which was a nuclear type that changed to a cell type during embryonic development. In the latest stages, remnants of the endosperm were observed as a net-like structure around the cotyledons. According to histological studies, divisions occurred faster in the basal cell than in the apical cell. The suspender was completely degenerated during the torpedo embryo stage. According to cyto-chemical studies, accumulation of starch grains in the seed coat was observed in the early stages of embryonic development. Moreover in the mature embryo, storage components contained protein bodies and lipid compounds. The seed coat consists of two layers, the outer layer of which contains calcium oxalate crystals. Fluorescent microscopic analysis showed that a cumulative lipid nature of waxes was observed in the grain. It seems that the study of features such as the pattern of organ formation in this plant can be effective in understanding the heterogeneity between species of the Zygophyllaceae family. Manuscript profile -
Open Access Article
4 - The effect of human gonadotropin and cinnamon hydroalcoholic extract on testes and seminal vesicles in adult male rats
elnaz shikhzade mamoii akbar karimi Ali Asghar Pileh VarianIssue 2 , Vol. 13 , Summer 2021Background and Aim: Since HCG stimulates the production of testosterone and increases androgen production, it is used in the treatment of infertility and in some cases, inflammatory conditions reduce its effect. In this study, due to the known anti-inflammatory effect o MoreBackground and Aim: Since HCG stimulates the production of testosterone and increases androgen production, it is used in the treatment of infertility and in some cases, inflammatory conditions reduce its effect. In this study, due to the known anti-inflammatory effect of cinnamon, it was used as a supplement to influence the effect of HCG on sexual activity of Adult male rats.Materials and Methods: In this experimental study, 24 Adult male Wistar rats were randomly divided into 4 groups of 6 including control group (daily subcutaneous saline), one HCG treatment group (50 IU / ml daily). Subcutaneous), treatment group with two hydroalcoholic extracts of cinnamon (30 mg / kg daily intraperitoneally) and treatment group with three HCG and hydroalcoholic extract of cinnamon (50 IU / ml daily subcutaneous injection and 30 mg / kg intravenous injection daily). Peritoneal) were divided. The rats were treated for 7 days, anesthetized after 14 days and after hematoxylin-eosin staining testes and seminal vesicles were examined by light microscopy. Data were analyzed using one-way ANOVA.Results: The results of the present study showed that the mean testicular and seminal vesicle weight, seminal vesicle diameter and seminal vesicle thickness and reproductive membrane thickness were significantly higher in the HCG and cinnamon receiving group (P <0.001). ).Discussion & Conclusion: Based on the results of this study, the combination of hydroalcoholic extract of cinnamon and HCG could lead to better development and testicular and seminal vesicle function in Adult male rats Manuscript profile -
Open Access Article
5 - Investigating the amounts of phenolic compounds, flavonoid production, and antioxidant activity in calluses obtained from different cultivars of (Cichorium intybus L.)
zahra zare Mahin Ghaemi Fariba AghiliIssue 1 , Vol. 16 , Winter 2024Natural antioxidant compounds can be a suitable substitute in pharmaceutical industries. Callus of (Cichorium intybus L) is a good source for antioxidant production. The research aim is to evaluate the amounts of phenolic compounds, flavonoid and antioxidant activity of MoreNatural antioxidant compounds can be a suitable substitute in pharmaceutical industries. Callus of (Cichorium intybus L) is a good source for antioxidant production. The research aim is to evaluate the amounts of phenolic compounds, flavonoid and antioxidant activity of plant in vitro and natural conditions. For this purpose, after preparing healthy seedlings from chicory seeds in (1.2 MS) culture medium, stem, leaf and root explants were prepared and cultured for callus formation in liquid MS with different hormone treatments. Methanolic extracts were prepared from each Callus.The phenolic content and flavonoid were determined by Folin – Ciocalteu and aluminum chloride methods and antioxidant activity by measuring the reduction of radical capacity by DPPH. The HPLC method was used to determine the type of phenolic compounds in the extracts.The results showed the highest amount of total phenol is 2.613 mg/g of the Callus weight related to the leaf explant with Kin (0.5mg/L), the highest amount of flavonoid is 0.828 mg/g related to the stem explant with 2,4-D (0.5mg /L) and the highest amount of total antioxidant was 89.35% related to the stem explant with BAP (1mg/L).The highest amount of chlorogenic acid was 0.513 mg/g related to the leaf explant with BAP (0.75 mg/L) and the highest amount of caffeic acid was 0.009 mg/g related to the stem explant with Kin (1 mg/L).The Callus obtained from stem and leaf explants, having a good level of phenolic and flavonoid compounds, can be used as a source of natural antioxidants instead of synthetic antioxidants. Manuscript profile -
Open Access Article
6 - Investigation of physiological properties and antioxidant activity of cumin by using of magnetic water and superabsorbent under water stress conditions
ali ashori manochher gholipoor ahmad gholami hamid AbbasdokhtIssue 2 , Vol. 15 , Spring 2023In order to investigate magnetic and superabsorbent water on some physiological traits and antioxidant activities of cumin (Cuminum cyminum L) under water stress, an experiment in two areas of Rahanjan and German of Shahroud city in factorial form in a randomized comple MoreIn order to investigate magnetic and superabsorbent water on some physiological traits and antioxidant activities of cumin (Cuminum cyminum L) under water stress, an experiment in two areas of Rahanjan and German of Shahroud city in factorial form in a randomized complete block design with Three replications were performed in the 1400-1399 crop year. The results of analysis of variance showed that the triple interaction effect of water requirement × magnetic water × superabsorbent was significant for the evaluated traits. The traits of chlorophyll a, chlorophyll b, total chlorophyll, carotenoids and anthocyanins were significant. Under moderate stress conditions, using normal water, no significant difference was observed between control and 200 kg / ha superabsorbent treatments. Most traits in Rahnjan region had higher values than German region. In general, the use of magnetic water and 200 kg / ha of superabsorbent in conditions of moderate drought stress (75% water requirement) caused physiological and biochemical increase of cumin. Manuscript profile -
Open Access Article
7 - Study of cytotoxic effect of Essential oil of Tymus vulgaris L by MTT and Trypan blue assay on breast cancer cell line MCF-7
Sasan Hajebi Abdolkarim Chehregani Rad Hossein LariyazdiIssue 4 , Vol. 9 , Winter 2017Breast Cancer is the most common cancer in the Iranian women. Essential oil of Thymus is containing anticancer compounds including thymol. The aim if this research was studding the effect of thymus essential oil on the breast cancer cell line MCF-7 using MTT and Trypan MoreBreast Cancer is the most common cancer in the Iranian women. Essential oil of Thymus is containing anticancer compounds including thymol. The aim if this research was studding the effect of thymus essential oil on the breast cancer cell line MCF-7 using MTT and Trypan blue methods, and comparing of its effect with taxol as a well-known anticancer agent. In this research, MCF-7 and Hu-02 cell lines were porched from IBRC institute. The cells were treated by different concentrations of Thymus essential oil and taxol in the times of 24, 48 and 72 hours. The cytotoxicity of the treatments was measured by means of MTT and trypan blue staining. Results showed that Thymus essential oil had cytotoxic effect, in different doses, on the MCF-7 cell line. The highest cytotoxic effect was measured in the group treated by 125 μg/ml of Thymus essential oil. The Thymus essential did not show any cytotoxic effect on the cells of HU-02. Results were also showed that the essential oil can increase the cytotoxicity effect of Taxol on the cancer cell line, thus it seems that is effective in breast cancer therapy; probably, it causes to cell death by apoptosis induction. Manuscript profile -
Open Access Article
8 - Effects of hydroalcoholic extract of Achilla millefolium and Salvia officinalis against human gastric Cancer cells
Omid Sofalian naser zare Saeed Latifi Sara Motalebinia Kobra HasanpourIssue 3 , Vol. 12 , Autumn 2020Treatment of cancer by medicinal plants has long been of interest to researchers. In the meantime, some plant species contain substances that, through apoptosis or necrosis, can inhibit or eliminate cancer cells. The family of mint and chicory are among the medicinal pl MoreTreatment of cancer by medicinal plants has long been of interest to researchers. In the meantime, some plant species contain substances that, through apoptosis or necrosis, can inhibit or eliminate cancer cells. The family of mint and chicory are among the medicinal plants that have many biological effects.The purpose of this study was to investigate the effect of Pennyroyal and Artemisia extract on gastric cancer cell line (AGS).In this study, aerial parts of plants were extracted. AGS cells were treated with the concentrations of Hydroatanolic extract (50-1000 g / ml) at 24, 48 and 72 hours intervals. Cell motility was estimated using MTT method and the rate of induction of apoptosis by flow cytometry with The Annexin-V .The results of MTT assay showed strong and dependent inhibitors of the concentration of cancer cell proliferation by various extracts of Achillea and Salvia. These extracts have a doseand time dependent anti cancer effect on AGS cells. In the study of apoptosis in treated cells, the extract of the Pennyroyal was more effective. Due to the cytotoxic effects of hydrotanolic extracts on AGS cells, these plants can be used as potential option for further studies on cancer treatment. Therefore, the purification of the active ingredient in these extracts and the determination of their effect mechanism is recommended. Manuscript profile -
Open Access Article
9 - Investigation of BAD Gene Expression Following Chloriazepoxide Effect on pregnancy In the hippocampus of mouse infants
Amin Dinarvand Mehrdad Hashemi Rasool Dinarvand Shabnam Movaseghi Mojtaba JafariniaIssue 2 , Vol. 11 , Autumn 2019Background: Chlorodiazepoxide is an anxiolytic agent commonly used by young people and pregnant women to reduce anxiety and control preeclampsia and eclampsia. Some studies have shown that this medication disrupts the functioning of the cholinergic system. Due to the in MoreBackground: Chlorodiazepoxide is an anxiolytic agent commonly used by young people and pregnant women to reduce anxiety and control preeclampsia and eclampsia. Some studies have shown that this medication disrupts the functioning of the cholinergic system. Due to the increased cellular damage of bad gene expression, the effect of cholorodiazepoxide use during pregnancy on bad gene expression in the hippocampus of neonatal rats was investigated. Methods: In this study, 9 female Wistar rats were pregnant and randomly divided into three groups: control, experimental (intraperitoneal injection of chlordiazoxoxide 10 mg / kg for 21 days) and carriers (saline). Two weeks after the birth, the brain of the neonates was removed from the skull and the expression of the propaoptotic gene of Bad was investigated. Results: The level of gene expression was analyzed by Rest software and a significant level of P Conclusion: The results of this study indicated that the administration of chlorodiazepoxide during pregnancy can cause neuronal damage in the hippocampus of Wistar rats. Manuscript profile -
Open Access Article
10 - The effect of umbilical cord stromal cells and chicory extract on the repair process of amputated sciatic nerve in male Wistar rats (Behavioral and electrophysiological study).
amirhosin fazlali Hayati Rodbari nasim gholamreza Kaka kazem parivar homayoon sadraiIssue 4 , Vol. 14 , Winter 2022Background and purpose: In this study, the therapeutic effect of umbilical cord blood cells with chicory extract on repair of severed sciatic nerve in adult male Wistar rats was evaluated by behavioral, electrophysiological study.Materials and Methods: After sciatic ner MoreBackground and purpose: In this study, the therapeutic effect of umbilical cord blood cells with chicory extract on repair of severed sciatic nerve in adult male Wistar rats was evaluated by behavioral, electrophysiological study.Materials and Methods: After sciatic nerve amputation, adult male rats were randomly divided into 4 groups of 7.Healthy rats, rats with sciatica without treatment intervention, rats with umbilical cord blood cells treated with chicory extract at the site of amputation, rats with umbilical cord blood cells injected at the site of injury.The rate of recovery was assessed by sensory motor activity of the sciatic nerve, electrophysiological studies.Results: Sciatic nerve motor evaluation, no control group returned to normal in the eighth week, cell therapy group was restored with chicory extract in the eighth week. The level of AMP in the eighth week after the restoration of the cell therapy group with a gentle slope indicates the recovery process of the cell therapy group.Counting the number of nerve fibers at an area of 1000 μm, the number of nerve fibers in the cell therapy groups increased in the eighth week after repair, compared with the control group and the PLGA membrane group. By the end of the eighth week, the sciatic nerve index (Hot Plate test), the healing process of the cell therapy group with chicory extract was more evident to other groups.Conclusion: Cord blood cell transplantation repairs sciatic nerve and chicory extract along with umbilical cord blood cells accelerates sciatic nerve repair. Manuscript profile
