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List of Articles
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Open Access Article
1 - Isolation and identification of dominant Salmonella species and their virulence factors in pets and their owners in Isfahan city
آتنابلالی Balali Dehkordi علی SharifzadehSalmonellosis is a common disease between humans and animals, and in some cases, animals carrying bacteria are a potential source of contamination for humans. The aim of this study was to assess the potential role of dogs and cats and their owners in epidemiology of sal MoreSalmonellosis is a common disease between humans and animals, and in some cases, animals carrying bacteria are a potential source of contamination for humans. The aim of this study was to assess the potential role of dogs and cats and their owners in epidemiology of salmonellosis in in Isfahan city. For this purpose, Rectal swabs of 115 asymptomatic dogs and cats from Isfahan city (2021) were cultured and evaluated by PCR. Salmonella isolates were serotyped and virulence gene and tested for antimicrobial susceptibility applying standard methods. The results obtained in the present study indicated that Salmonella spp. were isolated from 8% of cats,16% of cat owners and 12% of dog owners samples. 2 salmonella serotypes were observed including S. typhymurium (80%), and S. enteritidis (20%). The results showed that there is no significant relationship between age, condition of the digestive system and the environment of storage with the amount of salmonella infection in dogs and cats. Also, no connection was observed between the contamination of animals and their owners. According to the results, all cases were infected with invA and flic strains. According to the antibiogram tests performed, they were sensitive to ciprofloxacin, azithromycin, nalidixic acid, semi-sensitive to tetracycline, gentamicin, cephalothin and resistant to ampicillin, amoxicillin, cephalexin. Isolation of Salmonella spp. from asymptomatic cats and dogs makes them dangerous source of Salmonella and a treat for human and animal health. Manuscript profile -
Open Access Article
2 - Evaluation of metallo-betalactamase and carbapenemase activity in Pseudomonas aeruginosa strain isolated from clinical samples in Tehran
سمیه Mashayekhy فاطمه Noorbakhsh سحر Honarmand JahromiPseudomonas aeruginosa is an opportunistic pathogen and a major cause of nosocomial infections. Metallobetalactamases and carbapenemases are the most important factors in resistance to carbapenem drugs in Pseudomonas aeruginosa strains. The aim of this study was to eval MorePseudomonas aeruginosa is an opportunistic pathogen and a major cause of nosocomial infections. Metallobetalactamases and carbapenemases are the most important factors in resistance to carbapenem drugs in Pseudomonas aeruginosa strains. The aim of this study was to evaluate the activity of metallobetalactamase and carbapenemase in Pseudomonas aeruginosa isolated from clinical specimens. 49 strains of Pseudomonas aeruginosa isolated from patients admitted to the intensive care unit were identified by biochemical methods, then their antibiotic susceptibility was determined by Kirby- Bauer method. MBL producing strains were identified by phenotypic method combined disk test and KPC- producing strains were evaluated by MHT method. PCR method was also used to identify strains carrying VIM, SIM, GIM, SPM and IMP genes. Antibiotic resistance to ticarcillin, meropenem, gentamicin, ciprofloxacin, cefpime and ceftazidime were 89.8%, 51%, 44.9%, 67.4%, 93.9%, 95.9%, respectively. By phenotypic analysis combined disk test, 55.1% of the strains were identified as metallo-betalactamase producing strains. Also, 38.8% of carbapenemase producing strains were observed by MHT method. The frequencies of each of these gene’s VIM, SIM and GIM were 63.3%, 38.8%, 34.7%, respectively, and SPM and IMP genes were not observed in any of the strains in this study. Manuscript profile -
Open Access Article
3 - Synergistic effects of L-dopa and dopamine with melanocortin receptors (MC3/MC4) agonist on food intake in chickens
محمد Bameri مرتضی , Zendehdel بیتا Vazir نگار Panahi احمد asghariAppetite modulation includes a set of complex physiological mechanisms that affect different areas of the central nervous system. Previously, the role of dopaminergic and melanocortinergic systems in the central control of food intake in birds has been proven. The curre MoreAppetite modulation includes a set of complex physiological mechanisms that affect different areas of the central nervous system. Previously, the role of dopaminergic and melanocortinergic systems in the central control of food intake in birds has been proven. The current study was conducted with the aim of investigating the synergistic effects of dopaminergic system and melanocortinergic system on feeding behavior in layer chickens. In this study, three experiments were designed, so that each experiment included one control group and three treatment groups(n=12 in each group). In all groups, birds received intracerebroventricular injection of diluent solution or drug solution after 3 hours of food deprivation. In the first experiment, in order to determine the sub-effective dose of dopamine, normal saline and dopamine were injected with doses of 10, 20 and 40 nmol respectively in the test groups. In the second experiment, normal saline, L-dopa (dopamine precursor, 250 nmol), MTII (2.45 pmol, MC3/4 receptor agonist) and L-dopa + MTII were injected, and the third experiment was conducted as second experiment, but instead of L-dopa, dopamine (10 nmol) was injected either alone or in combination with MTII. After the injection, water and food were freely available to the birds and food consumption (gr) was measured based on the percentage of body weight. Based on the obtained results, the dose of 10 nmol of dopamine was determined as the sub-effective dose. It was also observed that the injection of sub-effective doses of dopamine, L-dopa and MTII alone had no effect on the food intake of chickens (p > 0.05), and only co-injections of L-dopa + MTII and dopamine + MTII caused a significant decrease in food intake in chickens (p<0.05). Based on the findings, it seems that there is probably a synergistic effect between the dopaminergic system and the melanocortinergic system in the control of food intake in chickens. Manuscript profile -
Open Access Article
4 - Protective effects of phoenixin 14 from the pathological point of view in experimental duodenal ulcer induced by indomethacin in rats
یاسر Zandeh‑Rahimi نگار Panahi سعید Hesaraki S.H Shirazi‑BeheshtihaPhoenixin-14 (PNX-14) is a newly identified neuropeptide with potential anti-inflammatory effects in the gastrointestinal tract. This study evaluated the protective effect of PNX-14 against the formation of duodenal ulcers caused by experimental indomethacin (IND). MorePhoenixin-14 (PNX-14) is a newly identified neuropeptide with potential anti-inflammatory effects in the gastrointestinal tract. This study evaluated the protective effect of PNX-14 against the formation of duodenal ulcers caused by experimental indomethacin (IND). Thirty-two male Sprague-Dawley rats were randomly assigned to four groups. They include negative control, IND (7.5 mg/kg subcutaneous indomethacin), FAM (7.5 mg/kg subcutaneous indomethacin followed by 40 mg /kg famotidine intraperitoneal), and PNX-14 (7.5 mg/kg subcutaneous indomethacin followed by 50 μg/kg intraperitoneal PNX-14). Outcome measures included macroscopic assessment of duodenal lesions and histopathological parameters. Selected parts of the duodenum were removed and placed in 10% formalin, and hematoxylin and eosin staining were prepared. The macroscopic grade of duodenal lesions in the PNX-14 group was significantly smaller than the IND group (P< 0.001). The components of tissue pathology lesions were significantly increased in the IND group. The wound's diameter and depth and the inflammation intensity in the PNX-14 group were significantly smaller than in the IND group (P<0.001). PNX-14 was superior to the FAM group in reducing inflammation. PNX-14 showed significant protective effects against IND-induced duodenal ulcer formation. These results show a promising therapeutic outcome for PNX-14 in treating inflammatory disorders of the gastrointestinal tract. Manuscript profile -
Open Access Article
5 - Isolation and optimization of growth conditions of yeasts with aflatoxin B1 reducing properties from local cheeses in Iran
غزال Yahyapour S.A.A Anvar مریم Ataee حامد Ahari حسین AskariAflatoxin is one of the most important causes of contamination in dairy products, which has shown resistance to removal through various methods. This study aimed to molecularly identify yeasts isolated from local cheeses and screen them based on their ability to reduce MoreAflatoxin is one of the most important causes of contamination in dairy products, which has shown resistance to removal through various methods. This study aimed to molecularly identify yeasts isolated from local cheeses and screen them based on their ability to reduce aflatoxin B1. To carry out this study, 32 samples of local cheese were prepared from different geographical locations of Tehran and Semnan provinces in Shahryar, Firoozkooh, Chetan and Garmsar. Then the yeast strains were isolated from the samples. Aflatoxin stock solution was prepared and added to the culture medium containing aflatoxin absorbing yeast strains. Adsorbed aflatoxin was measured indirectly by ELISA test. The data of this study showed that 3 strains including Code 6, Code 18, and Code 8 isolated from local cheeses had the ability to detoxify aflatoxin B1. Meanwhile, the highest reduction of aflatoxin B1 in the environment with 46.5% was done by strain code 6. In addition, it was found that the optimum growth temperature of these strains was between 28 and 36 ˚C and their optimum pH was 6.5-5.5. Since the biological removal of aflatoxin has many positive effects on dairy products from various sensory and health aspects, it seems that the yeast strains isolated in the present study were microorganisms with the potential to remove aflatoxin from dairy products, especially local cheese, and should be considered for further studies. Manuscript profile -
Open Access Article
6 - Molecular detection of pap GII, sfa and afa in Escherichia coli isolated from poultry colibacillosis and human urinary tract infection
خاطره Kafshdouzan تقی Zahraei Salehi بهار Nayeri FasaeiThe exploitation of virulence traits of Extra Pathogenic Escherichia coli (EXPEC) could be considered an applicable tool to describe the pathogenesis and design the new treatment and control methods. This study aimed to investigate the frequency of pap G II, Sfa, MoreThe exploitation of virulence traits of Extra Pathogenic Escherichia coli (EXPEC) could be considered an applicable tool to describe the pathogenesis and design the new treatment and control methods. This study aimed to investigate the frequency of pap G II, Sfa, and afa in Escherichia coli isolated from poultry colibacillosis as well as UTIs. Totally 320 Escherichia coli including 247 isolated from poultry colibacillosis from Semnan, Tehran and Gilan province, 53 isolated from the cloaca of apparently healthy birds from Semnan, and 20 from human UTI were evaluated for the presence of pap G II, Sfa, and afa by the colony hybridization assay. Statistical analysis was performed using the test and the P-value was considered <0.05. The results of this study showed among three adhesion genes, pap GII was the most common adhesins in both human and poultry source strains. Frequency of pap GII was 21.05%, 13.2%, and 35% in Escherichia coli isolated from poultry colibacillosis, UTI, and apparently healthy birds respectively. It was found that Escherichia coli isolated from human UTIs contained a higher frequency of the Sfa than poultry colibacillosis and afa was not detected in any of the groups. Escherichia coli isolated from poultry colibacillosis and human UTI showed high pap GII prevalence, which supports its role in the pathogenesis of EXPEC. Therefore, it can be an attractive target to develop novel anti-virulence therapeutics method and vaccine strategies. Manuscript profile
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