Synergistic effects of L-dopa and dopamine with melanocortin receptors (MC3/MC4) agonist on food intake in chickens

Subject Areas : Journal of Comparative Pathobiology

محمد Bameri ¹ (Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
مرتضی , Zendehdel ² (Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran)
بیتا Vazir ³ (Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
نگار Panahi ⁴ (Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
احمد asghari ⁵ (Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.)

Received: 2023-01-15 Accepted : 2023-04-19 Published : 2023-04-19

Keywords: Food Intake, Dopamine, Melanocortin, chicken,

Abstract :

Appetite modulation includes a set of complex physiological mechanisms that affect different areas of the central nervous system. Previously, the role of dopaminergic and melanocortinergic systems in the central control of food intake in birds has been proven. The current study was conducted with the aim of investigating the synergistic effects of dopaminergic system and melanocortinergic system on feeding behavior in layer chickens. In this study, three experiments were designed, so that each experiment included one control group and three treatment groups(n=12 in each group). In all groups, birds received intracerebroventricular injection of diluent solution or drug solution after 3 hours of food deprivation. In the first experiment, in order to determine the sub-effective dose of dopamine, normal saline and dopamine were injected with doses of 10, 20 and 40 nmol respectively in the test groups. In the second experiment, normal saline, L-dopa (dopamine precursor, 250 nmol), MTII (2.45 pmol, MC3/4 receptor agonist) and L-dopa + MTII were injected, and the third experiment was conducted as second experiment, but instead of L-dopa, dopamine (10 nmol) was injected either alone or in combination with MTII. After the injection, water and food were freely available to the birds and food consumption (gr) was measured based on the percentage of body weight. Based on the obtained results, the dose of 10 nmol of dopamine was determined as the sub-effective dose. It was also observed that the injection of sub-effective doses of dopamine, L-dopa and MTII alone had no effect on the food intake of chickens (p > 0.05), and only co-injections of L-dopa + MTII and dopamine + MTII caused a significant decrease in food intake in chickens (p<0.05). Based on the findings, it seems that there is probably a synergistic effect between the dopaminergic system and the melanocortinergic system in the control of food intake in chickens.

References:

1.Zendehdel, M., Ghashghayi, E., Hassanpour, S., Baghbanzadeh, A. and Jonaidi, H. Interaction between opioidergic and dopaminergic systems on food intake in neonatal layer type chicken. International Journal of Peptide Research and Therapeutics, 2016; 2: 283-92.

2. Zendehdel, M., Sardari, F., Hassanpour, S., Rahnema, M., Adeli, A. and Ghashghayi, E. Serotonin-induced hypophagia is mediated via α2 and β2 adrenergic receptors in neonatal layer-type chickens. British Poultry Science, 2017; 58: 298-304.

3. Rahmani, B., Ghashghayi, E., Zendehdel, M., Khodadadi, M. and Hamidi, B. The Crosstalk Between Brain Mediators Regulating Food Intake Behavior in Birds: A Review. International Journal of Peptide Research and Therapeutics, 2021; 27: 2349-2370.

4. McCarthy, J.C. and Siegel, P.B. A review of genetic and physiological Effects of selection in meat-type poultry. Anim. Breed, 1983; 51: 87-94.

5. Ikemoto, S. Dopamine reward circuitry: Two projection systems from ventral midbrain to the nucleus accumbens-olfactory tubercle complex. Brain Research Reviews, 2007; 56: 27-78.

6. Strange, P.G. The binding of agonists and antagonists to dopamine receptors. Biochemical Society Transactions, 1996; 24: 188-92.

7. Mahzouni, M., Zendehdel, M., Babapour, V. and Chackkar, S. Methylamine induced hypophagia is mediated via dopamine via D1 and D2 receptors in neonatal meat chick. Veterinary Research Communications, 2016; 40: 21-7.

8. Zendehdel, M. and Hassanpour, S. Ghrelin induced hypophagia is mediated by the B2 adrenergic receptor in chicken. The Journal of Physiological Sciences, 2014; 64: 383-391.

9. Zendehdel, M., Hamidi, F., Babapour, V., Mokhtarpouriani, K. and Mazaherinezhad fard, R. The effect of melanocortin (Mc3 and Mc4) antagonists on serotonin-induced food and water intake of broiler cockerels. Veterinary Sciences, 2012;13: 229-234.

10. Denbow, D.M., Van Krey, H.P., Lacy, M.P. and Dietrick, T.J. feeding, drinking and temperature of leghorn chickens: effects of ICVinjections of biogenic amine. Physiology & Behavior, 1983; 31: 85–90.

11. Alvaro, J. D., Taylor, J. R. and Duman, R. S. Molecular and behavioral interactions between central melanocortins and cocaine. The Journal of pharmacology and experimental therapeutics, 2003; 304(1): 391–399.

12. Schneeberger, M., Gomis, R. and Claret, M. Hypothalamic and brainstem neuronal circuits controlling homeostatic energy balance. The Journal of endocrinology, 2014 220(2): T25–T46.

13.Takeuchi, S. and Takahashi, S. Melanocortin receptor genes in the chicken--tissue distributions. General and comparative endocrinology, 1998; 112(2): 220–231.

14. Liu, H., Kishi, T., Roseberry, A. G., Cai, X., Lee, C. E., Montez, J. M., Friedman, J. M. and Elmquist, J. K. Transgenic mice expressing green fluorescent protein under the control of the melanocortin-4 receptor promoter. The Journal of neuroscience: the official journal of the Society for Neuroscience, 2003; 23(18): 7143–7154.

15. Strader, A. D., Schiöth, H. B. and Buntin, J. D. The role of the melanocortin system and the melanocortin-4 receptor in ring dove (Streptopelia risoria) feeding behavior. Brain research, 2003; 960(1-2): 112–121.

16. Qi, W., Ding, D. and Salvi, R. J. Cytotoxic effects of dimethyl sulphoxide (DMSO) on cochlear organotypic cultures. Hearing research, 2008; 236(1-2): 52–60.

17. Zendehdel, M., Hasani, K., Babapour, V., Mortezaei, S. S., Khoshbakht, Y. and Hassanpour, S. Dopamine-induced hypophagia is mediated by D1 and 5HT-2c receptors in chicken. Veterinary research communications, 2014; 38(1): 11–19.

18. Bameri, M., Zendedel kheybari, M., Vazir, B., Asghari, A. and Panahi, N. Evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin MC3/ MC4 receptors in regulating food intake of neonatal chick. Iranian Journal of Veterinary Science and Technology, 2021; 13(2): 37-45.

19. Rahmani, B., Mahdavi, K., Zendedel kheybari, M., Khodadadi, M., Keshavarz, M., Shahabi, M. and Baghbanzadeh, A. Role of central opioid receptors on serotonin-Induced hypophagia in the neonatal broilers. Iranian Journal of Veterinary Science and Technology, 2022; 14(1): 9-19.

20. Van Tienhoven, A.T. and Juhasz, L. The chicken telencephalon, diencephalon and mesencephalon in stereotaxic coordinates. Journal of Comparative Neurology, 1962; 118: 185-197.

21. Saito, E.-S., Kaiya, H., Tachibana, T., Tomonaga, S., Denbow, D.M., Kangawa, K. and Furuse, M. Inhibitory effect of ghrelin on food intake is mediated by the corticotropin-releasing factor system in neonatal chicks. Regulatory peptides, 2005; 125: 201-208.

22. Volkow, N. D., Wang, G. J. and Baler, R. D. Reward, dopamine and the control of food intake: implications for obesity. Trends in cognitive sciences, 2011; 15(1): 37–46.

23. Cadet, J. L., Jayanthi, S., McCoy, M. T., Beauvais, G. and Cai, N. S. Dopamine D1 receptors, regulation of gene expression in the brain, and neurodegeneration. CNS & neurological disorders drug targets, 2010; 9(5): 526–538.

24. Zendehdel, M. and Hassanpour, S. Central regulation of food intake in mammals and birds: a review. Neurotransmitter, 2014; 1: e251.

25. Khodadadi, M., Zendehdel, M., Baghbanzadeh, A. and Babapour, V. Consequence of dopamine D2 receptor blockade on the hyperphagic effect induced by cannabinoid CB1 and CB2 receptors in layers. British poultry science, 2017; 58(5): 585–593.

26. Taherian, M., Baghbanzadeh, A. and zendehdel, M. Dopamine- induced hypophagia is mediated via NMDA and mGlu1 receptors in chicken. Iranian Journal of Veterinary Medicine, 2016; 10(3): 191-199.

27. Zanganeh, F., Zendehdel, M., Panahi, N. and Asghari, A. Interconnection between Adrenergic and Dopaminergic Systems in Feeding Behavior in Neonatal Chicks. Archives of Razi Institute, 2021; 76(2): 345–358.

28. Zendehdel, M., Moosadoost, Y., Masoumi, R., Rostami, B., Shahir, M. and Hassanpour, S. Endogenous Nitric Oxide and Dopamine Regulate Feeding Behavior in Neonatal Layer-type Chickens. Annals of Animal Science, 2017; 17(4): 1029-1042.

29. GhandForoushan, M., Zendehdel, M. and Babpour, V. Interactions between Histamine H1 and H3 and Dopamine D1 Receptors on feeding behavior in chicken. Iranian Journal of Veterinary Medicine, 2017; 11(1): 63-73.

30. Wang, W., Guo, D. Y., Lin, Y. J. and Tao, Y. X. Melanocortin Regulation of Inflammation. Frontiers in endocrinology, 2019; 10: 683.

31. Takeuchi, S. and Takahashi, S. Melanocortin receptor genes in the chicken--tissue distributions. General and comparative endocrinology, 1988; 112(2): 220–231.

32. Greenman, Y., Kuperman, Y., Drori, Y., Asa, S. L., Navon, I., Forkosh, O., Gil, S., Stern, N. and Chen, A. Postnatal ablation of POMC neurons induces an obese phenotype characterized by decreased food intake and enhanced anxiety-like behavior. Molecular endocrinology (Baltimore, Md.), 2013; 27(7): 1091–1102.

33. Cui, H. and Lutter, M. The expression of MC4Rs in D1R neurons regulates food intake and locomotor sensitization to cocaine. Genes, brain, and behavior, 2013;12(6): 658–665.

34. Cooper, S. J., Al-Naser, H. A. and Clifton, P. G. The anorectic effect of the selective dopamine D1-receptor agonist A-77636 determined by meal pattern analysis in free-feeding rats. European journal of pharmacology, 2006; 532(3): 253–257.

35.       Yoon, Y. R. and Baik, J. H. Melanocortin 4 Receptor and Dopamine D2 Receptor Expression in Brain Areas Involved in Food Intake. Endocrinology and metabolism (Seoul, Korea), 2015; 30(4): 576–583.