List of Articles Saeed Hesaraki

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  1 - Purification and Determination of some Characters of Dominant Isoform of GP96 Molecules from Mice Fibrosarcoma Cell Line (WEHI164)
  عقیل Tabar Molla Hassan زهیر Hassan علی Mostafaie سعید Hesaraki علی Taravati
  GP96 protein is a heat shock protein (HSP) with an important role in cellular immunity. Tumorderived gp96 has entered clinical trials for cancer immunotherapy. For purification of dominantisoform of gp96 molecules from mice fibrosarcoma cell line, Cultured WEHI164 cell More

  GP96 protein is a heat shock protein (HSP) with an important role in cellular immunity. Tumorderived gp96 has entered clinical trials for cancer immunotherapy. For purification of dominantisoform of gp96 molecules from mice fibrosarcoma cell line, Cultured WEHI164 cell line werelysed and supernatants of cell extract were precipitated by Amoniume sulfate , In the next stage,purification was done through affinity and ione exchange chromatography. Molecular Weight ofpurified protein was estimated by SDS-PAGE and its nature was confirmed by western blot. Theresult of this study shows that, purified molecule has molecular weight between 66-68 kd whichindicates another different isoform of this molecule; a result confirmed by western blot. Manuscript profile
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  2 - Biomechanical and histopathological evaluation of two surgical methods, nerve anastomosis and tendon transfer with each other in function of rabbits limbs
  غلامرضا عابدی احمد اصغری رحیم علیزاده سعید حصارکی سپهر خاشعی
  Tendon transfer is to change the natural location to a new location for attachment of a tendon ofa muscle's activity. This study was conducted to biomechanical and histopathological evaluatethe function of rabbits drop foot which were operated for nerve anastomosis and More

  Tendon transfer is to change the natural location to a new location for attachment of a tendon ofa muscle's activity. This study was conducted to biomechanical and histopathological evaluatethe function of rabbits drop foot which were operated for nerve anastomosis and tendon transferwith each other. Twenty white New Zealand male and healthy rabbits were chosen and dividedin to 4 groups. First group (Witness): were operated and only cut the sciatic nerve. Secondgroup: were operated and cut the sciatic nerve, then anastomosed. Third group: were operatedand cut the sciatic nerve, then tendon transfer were done whit out any Anastomosis. Fourthgroup: were operated and cut the sciatic nerve and then anastomosed. Then tendon transferswere done. Biomechanical evaluation in day 45 after surgery on bone samples. In biomechanicalassessment, about stiffness factor, those groups which operated by tendon transfer method, weneed less power. About fracture, in those groups which operated by tendon transfer method,more force in necessary to break. In those groups which operated by tendon transfer method, therate of calcium is much more than matrix because their feet can tolerate their weight incomparison, in those groups which operated without tendon transfer; the rate of calcium is lessthan matrix because of osteodystrophy. In pathological assessment, zenker necrosis and atrophyin both groups were detected but the acutness in witness group is more obviously. It could beclaimed that utilizing two surgical methods; nerve anastomosis and tendon transfer with eachother had more effect on function of paralyzed limbs. Manuscript profile
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  3 - Diagnosis and Scoring of a vaginal SCC by immunohistochemistry in a cow
  سعید حصارکی علیرضا عزیزی سراجی
  An eight-year-old, female holstein cow, weighting 457 kg was presented with a 7.5×5×8 cmplaque on the surface of the vagina. The mass was removed surgically and Squamous CellCarcinoma (SCC). Immunohistochemically, positivity for AE1/AE3, CK5/6, CK8 and PCNAr More

  An eight-year-old, female holstein cow, weighting 457 kg was presented with a 7.5×5×8 cmplaque on the surface of the vagina. The mass was removed surgically and Squamous CellCarcinoma (SCC). Immunohistochemically, positivity for AE1/AE3, CK5/6, CK8 and PCNArevealed that this tumor was originated from keratinocytes and high grade. ThereforeImmunohistochemistry can help the clinicians in Prognosis and Treatment of referred Cases. Manuscript profile
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  4 - Protective effects of black Iranian tea on cadmium- induced hepatotoxicity in rats
  M.R Mohammadi Malayeri, سعید Hesaraki, پیام Jamal livani,
  Useful effects of black tea in treatment of many diseases have been shown . the protectiveeffects of tea are possibly related to its antioxidant properties or its inhibition of lipidoxidation. The aim of the present study was to investigate hepatoprotective effects of b More

  Useful effects of black tea in treatment of many diseases have been shown . the protectiveeffects of tea are possibly related to its antioxidant properties or its inhibition of lipidoxidation. The aim of the present study was to investigate hepatoprotective effects of blackIranian tea on cadmium(cd)-induced hepatotoxicity .For this purpose 30 male wistar rats wererandomly divided to six study groups including 1-negative control, 2-treatment control (received10% black tea brewed drink (BTBD)),3-positive control which received cd(5 mg/kg bodyweight (bw.)/day) and 4 to 6-treatment groups in which BTBD was administered orally (2,5 and10%) with oral cd(5mg/kg bw./day). The rats were sacrificed after 4 weeks and blood sampleswere collected to assess liver biomarkers. liver samples were fixed in buffered formalin solution. They were then conventionally embedded in paraffin and stained with Hematoxilin - Eosin forpathology studies as well. In positive control group, activities of alanine transaminase(ALT),aspartate transaminase(AST) and alkaline phosphatase (ALP) significantly increased in comparewith negative control group. Granular degeneration, chromatin and nucleus concentration andnecrosis were evidenced in pathological study of the liver sections of this group. On the otherhand, 5 and 10% BTBD administration significantly prevented ALT,AST and ALP increasingactivity. Pathological study confirmed the latter serological results. Whereas, 2% BTBDadministration didn't have any significant protection. in conclusion, our results suggest that dailyblack tea drinking may prevent cd-induced hepatic lesions. Manuscript profile
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  5 - Compare the effect red and green low level laser light accelerates the healing of the incision in the skin after the suturing in hamsters
  GH. R. Abedi, احمد Asghari, S. Hesaraki, A. Nasireian, هادی Hajizadeh, نیما Toghani,
  Accelerated wound healing was the first application for this type of laser. In terms of green lightlaser ability which whether it can enhance wound healing or not there is some disagreement. thisstudy was done in order to compare the green and red light lasers (less pow More

  Accelerated wound healing was the first application for this type of laser. In terms of green lightlaser ability which whether it can enhance wound healing or not there is some disagreement. thisstudy was done in order to compare the green and red light lasers (less powered one) in terms ofaccelerated wound healing which can be done surgically on the skin. In this study thirty healthy,mature hamster from suyrian strain was selected and randomly divided into three different groupas below: A: control group, B: group which was under the radiation of low level red laser, C:group which was under the radiation of low level green laser. After the induction of generalanesthesia, the straight incision at the length of two cm was created in the dorsal zone of animal,then by means of nylon 5-0 was sutured in a simple manner. In the group A the cutted sitewithout any intervention was remained intact in order to proceed first type of wound healing, inthe group B (by red laser) and C (by means of green laser) the cutted site was treated with thevelocity of 0.5 J/cm2 during one minute by constant movement of lasers on the surface of incisionwhich was continued up to six days. Sampling was done in the 3th 7th, and 12th days. Theaverage wound healing in groups B and C were significantly higher than the control group, butbetween the two green and red laser, there was no meaningful difference. Although the red lasera little bit was good during operation compared with the green one. Less powered laseraccelerates the process of wound healing. In those situations which the case is resistance towound healing we can use less powered lasers. Manuscript profile
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  6 - Histopathologic evaluation of pulmonary lesions in camel in Iran (Camelus dromedarius)
  A. Chitgar, ایرج Sohrabi Haghdost, J. Jamshidian, S. Hesaraki,
  Pulmonary infections are of important diseases in camel that jeopardize the health of the animaland decrease its production efficiency. Considering that prevention and treatment of pulmonaryinfections necessitate the knowledge of respiratory diseases and the fact that l More

  Pulmonary infections are of important diseases in camel that jeopardize the health of the animaland decrease its production efficiency. Considering that prevention and treatment of pulmonaryinfections necessitate the knowledge of respiratory diseases and the fact that little information isavailable in this regard in camel, the present study was conducted to investigate the pulmonarylesions using histopathological methods in camels slaughtered. The respiratory tracts of thecamels were thoroughly inspected for lesions. Specimens were fixed in 10% formalin. Next, thesamples were stained using Hematoxilin and eosin stain (H&E) and were evaluated. Theoccurrence of pulmonary lesions was 79.6% (236/447). The occurrence acute interstitialpneumonia, chronic interstitial pneumonia, bronchopneumonia, bronchiolitis, pleuritis andatelectasis were 52.8% (236/447), 5.4% (24/447), 7.8% (35/447), 6.7% (30/447), 3.4% (15/447)and 15.2% (68/447), respectively. The occurrence of pulmonary lesions, acute interstitialpneumonia and bronchopneumonia was higher in fall and winter than spring and summer (P <0.05). In conclusion, the present study showed high occurrence of pulmonary lesions in camels.Moreover, the results revealed higher occurrence of pulmonary lesions in cold seasons. Manuscript profile
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  7 - ارزیابی فعالیت آنزیم‌های شاخص آسیب قلبی پس از حذف پریکارد در خرگوش
  O. Samiee Amlashi, , Gh. Abedi محمد Nasrollahzadeh Masouleh, , S. Hesaraki
  پریکاردیکتومی یک شیوه درمانی موثر برای برخی از بیماری­های قلبی است و استفاده از بیومارکرهای قلبی شیوه مهمی برای ارزیابی اثرات جراحی بر سلامت قلب هستند. در این راستا، ﻛـﺮاﺗﻴﻦﻛﻴﻨﺎز، آﺳﭙﺎرﺗﺎت­آﻣﻴﻨﻮﺗﺮاﻧﺴﻔﺮاز و ﻻﻛﺘـﺎت­دﻫﻴﺪروژﻧﺎز ﺑﻪ­ﻋﻨﻮان آﻧﺰﻳﻢ­ﻫﺎی وﻳﮋه آﺳ More

  پریکاردیکتومی یک شیوه درمانی موثر برای برخی از بیماری­های قلبی است و استفاده از بیومارکرهای قلبی شیوه مهمی برای ارزیابی اثرات جراحی بر سلامت قلب هستند. در این راستا، ﻛـﺮاﺗﻴﻦﻛﻴﻨﺎز، آﺳﭙﺎرﺗﺎت­آﻣﻴﻨﻮﺗﺮاﻧﺴﻔﺮاز و ﻻﻛﺘـﺎت­دﻫﻴﺪروژﻧﺎز ﺑﻪ­ﻋﻨﻮان آﻧﺰﻳﻢ­ﻫﺎی وﻳﮋه آﺳﻴﺐ قلبی از اﻫﻤﻴﺖ ﺧﺎﺻﻲ ﺑﺮﺧﻮردارند. هدف از این تحقیق بررسی فعالیت این آنزیم­ها بعد از پریکاردیکتومی در خرگوش­های سالم بود. تعداد 12 سر خرگوش سفید نیوزلندی نر هم­سن با وزن تقریبی 3/0±5/1 کیلوگرم به­ طور تصادفی به دو گروه شاهد و مورد تقسیم شدند. خرگوش­های گروه مورد، عمل پریکاردیکتومی و خرگوش­های گروه شاهد فقط تحت عمل توراکتومی قرار گرفتند. به­ منظور بررسی تغییرات آنزیم­های کراتین­کیناز، لاکتات­ دهیدروژناز، آسپارتات ­آمینوترانسفراز، نمونه­ گیری خون، از تمامی خرگوش­های جراحی شده در هفته ­های اول، دوم، سوم و چهارم بعد از عمل جراحی، انجام شد. براساس نتایج به­دست آماده، مقادیر کراتین­ کیناز در هفته ی اول اختلاف معنی ­داری نسبت به گروه شاهد داشت (05/0 > p). مقادیر کراتین­ کیناز گروه مورد در هفته اول در مقایسه با هفته ­های دوم، سوم و چهارم از نظر آماری افزایش معنی ­داری را نشان داد (05/0 > p). میزان لاکتات­ دهیدروژناز و آسپارتات­ آمینوترانسفراز گروه مورد در هفته­ دوم افزایش معنی ­داری داشتند (05/0 > p). مقادیر لاکتات ­دهیدروژناز و آسپارتات آمینوترانسفراز گروه مورد در هفته دوم در مقایسه با هفته ­های اول، سوم و چهارم اختلاف معنی­داری را نشان دادند (05/0 > p). به­طورکلی می­توان نتیجه گرفت که پریکاردیکتومی باعث افزایش موقت فعالیت آنزیم­های شاخص آسیب قلبی در هفته­ اول و دوم می­شود و بعد از پایان هفته دوم سطوح آنزیم­های کراتین­ کیناز، آسپارتات­آمینوترانسفراز و لاکتات ­دهیدروژناز به حد طبیعی خود برگشته و این افزایش مقادیر دائمی نیستند. Manuscript profile
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  8 - Study of kidney anatomy and microscopic of renal proximal and distal tubules of middle and posterior part of kidney tissue in Oncorhynchus mykiss and Astronotus ocellatus
  . S Mohammadalipour Iraj pousty , S Hesaraki
  The present study was conducted to compare the histology of renal proximal tubules (I) and (II) in two species (Rainbow trout and Oscar). Rainbow trout, known as Oncorhynchus mykiss scientific name from the Salmonidae family which is cultivated and freshwater. Oscar fis More

  The present study was conducted to compare the histology of renal proximal tubules (I) and (II) in two species (Rainbow trout and Oscar). Rainbow trout, known as Oncorhynchus mykiss scientific name from the Salmonidae family which is cultivated and freshwater. Oscar fish, the scientific name is Astronotus Ocellatus, which is one of the most well-known ornamental fish of the Chichlidae family. Five piece of the two fish species were selected randomly and their macroscopic specification and morphometric measurements were recorded. Samples were fixed in %10 formalin buffer and the cuts were prepared with a thickness of five micron and then they were stained by Hematoxylin and eosin and Periodic acid–Schiff method and studied by light microscopy. The data were analyzed with SPSS software and T-Test statistical test (P <0.05). Proximal and distal tubes were detected and the variables were measured. The size of the cell nucleus and the outer diameter of the proximal (I) had a significant difference (P<0.01). There has been significant difference in the number and the size of cell and its nucleus, the inner and outer diameter of the proximal II between two species (P<0.001). The number, cell size of the distal tubules has been significant difference between two species (P<0.01). The size of the cell nucleus, the inner and outer diameter of the distal tubules have been significant difference between two species (P<0.001). According to the results, the anatomy and histology of kidney tissue are different in the Rainbow trout and Oscar fish.  Manuscript profile
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  9 - Evaluation of healing effect of clove nanofibers in comparison with poly-caprolactone nanofibers in experimental skin wounds
  M. Ashjazadeh, A. Jahandideh, G. Abedi, A. Akbarzadeh, S. Hesaraki,
  To date, the anti-inflammatory and antimicrobial effect of clove extract has been proven, which makes it one of the treatments that can be used in wound healing. Comparing the healing effect with other healing aids makes clove extract stand out among wound healers. In t More

  To date, the anti-inflammatory and antimicrobial effect of clove extract has been proven, which makes it one of the treatments that can be used in wound healing. Comparing the healing effect with other healing aids makes clove extract stand out among wound healers. In this study, histopathological examination of clove nanofibers (eugenol) was performed in comparison with polycaprolactone nanofibers in other experimental thicknesses created in rat skin. In 45 adult Wistar rats, a dorsal ulcer induced. They are then randomly divided into three groups of 15 (observed group, eugenol nanofibers and polycaprolactone), then each into five other groups of 5 (fraternity sample groups on days seven, 14, and 21) Divide, collagen, epithelial thickness, scar, leukocytes infiltration, edema, acute hemorrhage and stretch test were evaluated. In the eugenol treatment group, epithelialization and collagen index increased significantly (P <0.05) from days 7 to 21 from the other two budget groups. The results show that clove nanofibers accelerated the healing process in thickened skin function compared to polycaprolactone and the control group. Manuscript profile
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  10 - Observation of anti-cancer effects of aflatoxin B1 in breast tumor category 4T1 of mice by injection around the tumor
  R. Mousazadeh, S. Hesaraki, M. Bayat, A. Jahandideh, J. Hashemi,
  Treatment of breast cancer has many problems due to the systemic effects of chemotherapy. The use of certain molecules with cell killing effects, such as aflatoxin B1, can have fatal effects on breast cancer cells. In this study, we investigated the inhibitory effect of More

  Treatment of breast cancer has many problems due to the systemic effects of chemotherapy. The use of certain molecules with cell killing effects, such as aflatoxin B1, can have fatal effects on breast cancer cells. In this study, we investigated the inhibitory effect of topical aflatoxin B1 use on breast cancer. Class 4T1 cancer cells were inoculated subcutaneously in female mice. Doses of 50 and 500 μg / ml of aflatoxin B1 were then injected around the tumors in comparison with doxorubicin. The ratio of tumor size to animal weight in different groups was evaluated along with histopathology by immunofluorescence on Ki-67 and VEGF indices. Aflatoxin B1 significantly reduces tumor volume, necrosis and suppresses angiogenesis in the tumor. However, liver metastasis occurred in all groups. Aflatoxin B1 in topical injections of 50 and 500 μg / ml around the tumor has anti-cancer effects on the breast. But it can not prevent metastasis.Aflatoxin-induced hepatotoxicity was also observed. Manuscript profile
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  11 - Investigating the effect of Scenedesmus acuminates algae extract on healing wounds infected with Staphylococcus aureus in a rat model
  هومن Akasheh, علیرضا Jahandideh امیر اقبال Khajerahimi شاپور Kakoolaki سعید Hesaraki
  10.30495/jcp.2022.21487
  The purpose of this study is to use a seaweed called Scenedesmus acuminates to reduce infection and heal wounds. Sixty rats were divided into four treatment groups, and sampling was done on the third, seventh, and fourteenth days after wound formation. No treatment was More

  The purpose of this study is to use a seaweed called Scenedesmus acuminates to reduce infection and heal wounds. Sixty rats were divided into four treatment groups, and sampling was done on the third, seventh, and fourteenth days after wound formation. No treatment was done on the first group, the control group. The second group was treated with 25% zinc oxide as the drug of choice. The third and fourth groups were treated with 3% and 7% Scenedesmus acuminates seaweed ointment. The back wounds of mice were infected with 50 microliters of 2x108 CFU/mL Staphylococcus containing suspension. Histopathology and immunohistochemistry (CD68) were performed to compare the number of macrophages on tissue samples. On the third day after wounding, macrophages (CD68) were present in the algae group by 7% more than in the other groups. On the seventh day, fibroplasia and collagen synthesis in the algae group was 7% better than in the other two groups. On the 14th day, the accumulation of new blood vessels in the algae was 7% more than the others. The best result regarding the CD68 (macrophage) molecule expression level was seen in the 7% algae group on the 7th and 14th days. On the third, seventh, and fourteenth days, when macrophages are helpful, algae groups were better than others. The use of algae ointment can reduce the bacterial load of the wound and is better than the administration of zinc oxide.  Manuscript profile
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  12 - Protective effects of phoenixin 14 from the pathological point of view in experimental duodenal ulcer induced by indomethacin in rats
  یاسر Zandeh‑Rahimi نگار Panahi سعید Hesaraki S.H Shirazi‑Beheshtiha
  10.30495/jcp.2022.21981
  Phoenixin-14 (PNX-14) is a newly identified neuropeptide with potential anti-inflammatory effects in the gastrointestinal tract. This study evaluated the protective effect of PNX-14 against the formation of duodenal ulcers caused by experimental indomethacin (IND). More

  Phoenixin-14 (PNX-14) is a newly identified neuropeptide with potential anti-inflammatory effects in the gastrointestinal tract. This study evaluated the protective effect of PNX-14 against the formation of duodenal ulcers caused by experimental indomethacin (IND). Thirty-two male Sprague-Dawley rats were randomly assigned to four groups. They include negative control, IND (7.5 mg/kg subcutaneous indomethacin), FAM (7.5 mg/kg subcutaneous indomethacin followed by 40 mg /kg famotidine intraperitoneal), and PNX-14 (7.5 mg/kg subcutaneous indomethacin followed by 50 μg/kg intraperitoneal PNX-14). Outcome measures included macroscopic assessment of duodenal lesions and histopathological parameters. Selected parts of the duodenum were removed and placed in 10% formalin, and hematoxylin and eosin staining were prepared. The macroscopic grade of duodenal lesions in the PNX-14 group was significantly smaller than the IND group (P< 0.001). The components of tissue pathology lesions were significantly increased in the IND group. The wound's diameter and depth and the inflammation intensity in the PNX-14 group were significantly smaller than in the IND group (P<0.001). PNX-14 was superior to the FAM group in reducing inflammation. PNX-14 showed significant protective effects against IND-induced duodenal ulcer formation. These results show a promising therapeutic outcome for PNX-14 in treating inflammatory disorders of the gastrointestinal tract. Manuscript profile
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  13 - Evaluation of the performance of titanium dioxide nanocomposite scaffold compared to hydroxyapatite on the healing of rabbit femoral bone defects
  حسین Sonbolekar علیرضا Jahandideh احمد Asghari سعید Hesaraki, ابوالفضل Akbarzadeh,
  10.30495/jcp.2023.22491
  This study was conducted with the aim of investigating the effectiveness of titanium dioxide nanocomposite scaffolds for the reconstruction of femur defects in rabbits. To investigate the effect of nanocomposites on the healing processes of broken bone (bone defect). 18 More

  This study was conducted with the aim of investigating the effectiveness of titanium dioxide nanocomposite scaffolds for the reconstruction of femur defects in rabbits. To investigate the effect of nanocomposites on the healing processes of broken bone (bone defect). 18 New Zealand adult male white rabbits weighing 3 kg were studied. A cylindrical defect with a diameter of 6 x 5 mm was created in the femur of rabbits. The animals were divided into three experimental groups. The experimental groups after the defect were created included control, control (hydroxyapatite) and treatment (titanium dioxide). On the 15th and 45th days after surgery, the animals were killed and a sample was taken from the bone defect site and placed in 10% buffered formalin. 5 μm sections were evaluated after general and trichrome staining. Improvement of inflammation on day 15 and formation of new bone on day 45 in the nanocomposite group was the best in terms of ossification repair unit and reduction of inflammation. Nanocomposite had a significant recovery function in bone regeneration of defective areas. Manuscript profile