Molecular detection of pap GII, sfa and afa in Escherichia coli isolated from poultry colibacillosis and human urinary tract infection

Subject Areas : Journal of Comparative Pathobiology

خاطره  Kafshdouzan ¹ (Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.)
تقی  Zahraei Salehi ² (Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iran.)
بهار  Nayeri Fasaei ³ (Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iran.)

Received: 2022-12-26 Accepted : 2023-04-10 Published : 2022-05-10

Keywords: Escherichia coli, Virulence factors, Fimbrial adhesins,

Abstract :

The exploitation of virulence traits of Extra Pathogenic Escherichia coli (EXPEC) could be considered an applicable tool to describe the pathogenesis and design the new treatment and control methods.  This study aimed to investigate the frequency of pap G II, Sfa, and afa in Escherichia coli isolated from poultry colibacillosis as well as UTIs. Totally 320 Escherichia coli including 247 isolated from poultry colibacillosis from Semnan, Tehran and Gilan province, 53 isolated from the cloaca of apparently healthy birds from Semnan, and 20 from human UTI were evaluated for the presence of pap G II, Sfa, and afa by the colony hybridization assay. Statistical analysis was performed using the  test and the P-value was considered <0.05. The results of this study showed among three adhesion genes, pap GII was the most common adhesins in both human and poultry source strains. Frequency of pap GII was 21.05%, 13.2%, and 35% in Escherichia coli isolated from poultry colibacillosis, UTI, and apparently healthy birds respectively. It was found that Escherichia coli isolated from human UTIs contained a higher frequency of the Sfa than poultry colibacillosis and afa was not detected in any of the groups.   Escherichia coli isolated from poultry colibacillosis and human UTI showed high pap GII prevalence, which supports its role in the pathogenesis of EXPEC. Therefore, it can be an attractive target to develop novel anti-virulence therapeutics method and vaccine strategies.

References:

1.Kafshdouzan K, Zahraei Salehi T, Nayeri B, Madadgar O, Yamasaki S, Hinenoya A, et al. Distribution of virulence associated genes in isolated Escherichia coli from avian colibacillosis. Iranian Journal of Veterinary Medicine. 2013;7(1):1-6.

2. Nazemi A, Naderi M, Jafarpour M, Mirinargesi M, Sharifi S. The Detection of Fimbrial Pathogenic Genes in E. coli Strains Isolated from Patients with Urinary Tract Infection. Medical Laboratory Journal. 2010;4(2):31-7.

3. La Ragione R, Woodward MJ. Virulence factors of Escherichia coli serotypes associated with avian colisepticaemia. Research in veterinary science. 2002;73(1):27-35.

4. Eslami M, Ghanbarpour R. Determination of P, S and Afa fimbria coding genes in Escherichia coli isolates from urinary tract infections. Journal of Isfahan Medical School. 2015;33(331):546-53.

5. Buberg ML, Mo SS, Sekse C, Sunde M, Wasteson Y, Witsø IL. Population structure and uropathogenic potential of extended-spectrum cephalosporin-resistant Escherichia coli from retail chicken meat. BMC microbiology. 2021;21(1):1-15.

6. Ewers C, Antão E-M, Diehl I, Philipp H-C, Wieler LH. Intestine and environment of the chicken as reservoirs for extraintestinal pathogenic Escherichia coli strains with zoonotic potential. Applied and environmental microbiology. 2009;75(1):184-92.

7. Mellata M, Johnson J, Curtiss III R. Escherichia coli isolates from commercial chicken meat and eggs cause sepsis, meningitis and urinary tract infection in rodent models of human infections. Zoonoses and public health. 2018;65(1):103-13.

8. Mitchell NM, Johnson JR, Johnston B, Curtiss III R, Mellata M. Zoonotic potential of Escherichia coli isolates from retail chicken meat products and eggs. Applied and environmental microbiology. 2015;81(3):1177-87.

9. Gupta S, Kumar P, Rathi B, Verma V, Dhanda RS, Devi P, et al. Targeting of Uropathogenic Escherichia coli papG gene using CRISPR-dot nanocomplex reduced virulence of UPEC. Scientific reports. 2021;11(1):1-14.

10. Le Bouguenec C, Archambaud M, Labigne A. Rapid and specific detection of the pap, afa, and sfa adhesin-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction. Journal of clinical microbiology. 1992;30(5):1189-93.

11. Wu Y, Hinenoya A, Taguchi T, Nagita A, Shima K, Tsukamoto T, et al. Distribution of virulence genes related to adhesins and toxins in shiga toxin-producing Escherichia coli strains isolated from healthy cattle and diarrheal patients in Japan. Journal of Veterinary Medical Science. 2010:1001200133-.

12. Sarowska J, Futoma-Koloch B, Jama-Kmiecik A, Frej-Madrzak M, Ksiazczyk M, Bugla-Ploskonska G, et al. Virulence factors, prevalence and potential transmission of extraintestinal pathogenic Escherichia coli isolated from different sources: recent reports. Gut pathogens. 2019;11(1):1-16.

13. Ewers C, Li G, Wilking H, Kieβling S, Alt K, Antáo E-M, et al. Avian pathogenic, uropathogenic, and newborn meningitis-causing Escherichia coli: how closely related are they? International journal of medical microbiology. 2007;297(3):163-76.

14. Johnson TJ, Wannemuehler Y, Johnson SJ, Stell AL, Doetkott C, Johnson JR, et al. Comparison of extraintestinal pathogenic Escherichia coli strains from human and avian sources reveals a mixed subset representing potential zoonotic pathogens. Applied and environmental microbiology. 2008;74(22):7043-50.

15. Vandemaele FJ, Mugasa JP, Vandekerchove D, Goddeeris BM. Predominance of the papGII allele with high sequence homology to that of human isolates among avian pathogenic Escherichia coli (APEC). Veterinary microbiology. 2003;97(3-4):245-57.

16. Roberts JA, Kaack MB, Baskin G, Chapman MR, Hunstad DA, Pinkner JS, et al. Antibody responses and protection from pyelonephritis following vaccination with purified Escherichia coli PapDG protein. The Journal of urology. 2004;171(4):1682-5.

17. Ashrafi F, Mehrabadi JF, Siadat SD, Aghasadeghi MR. Expression and purification of the uropathogenic Escherichia coli PapG protein and its surface absorption on Lactobacillus reuteri: implications for surface display system vaccines. Jundishapur Journal of Microbiology. 2015;8(9).

18. Knöbl T, Gomes TAT, Vieira MAM, Ferreira F, Bottino JA, Ferreira AJP. Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil. Brazilian Journal of Microbiology. 2006;37:379-84.

19. Stordeur P, Marlier D, Blanco J, Oswald E, Biet F, Dho-Moulin M, et al. Examination of Escherichia coli from poultry for selected adhesin genes important in disease caused by mammalian pathogenic E. coli. Veterinary microbiology. 2002;84(3):231-41.

20. Delicato ER, de Brito BG, Gaziri LCJ, Vidotto MC. Virulence-associated genes in Escherichia coli isolates from poultry with colibacillosis. Veterinary microbiology. 2003;94(2):97-103.

21. Rodriguez-Siek KE, Giddings CW, Doetkott C, Johnson TJ, Nolan LK. Characterizing the APEC pathotype. Veterinary research. 2005;36(2):241-56.

22. Qin X, Hu F, Wu S, Ye X, Zhu D, Zhang Y, et al. Comparison of adhesin genes and antimicrobial susceptibilities between uropathogenic and intestinal commensal Escherichia coli strains. PLoS One. 2013;8(4):e61169.

23. Biggel M, Xavier BB, Johnson JR, Nielsen KL, Frimodt-Møller N, Matheeussen V, et al. Horizontally acquired papGII-containing pathogenicity islands underlie the emergence of invasive uropathogenic Escherichia coli lineages. Nature communications. 2020;11(1):1-15.

24. Farshad S, Emamghorashi F, Amin Shahidi M. Epidemiologic evaluation of virulence genes, pap، sfa، cnf-1، hlyin E. colistrains isolated from children withurinary tract infection. Iranian Journal of Medical Microbiology. 2009;2(3):31-7.