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Open Access Article
1 - Extraction, purification, characterization and antioxidant activities of heat-resistance phenolic compounds from lemon pulp
Yasar Ipek Tevfik Ozen Ibrahim DemirtasLemon as a citrus species is widely cultivated in the Mediterranean region, Turkey. The lemon pulp has been characterized as a rich source of phenolic and flavonoid compounds, phenolic acid as well as novel coumarin derivatives. It is usually obtained within the juice p MoreLemon as a citrus species is widely cultivated in the Mediterranean region, Turkey. The lemon pulp has been characterized as a rich source of phenolic and flavonoid compounds, phenolic acid as well as novel coumarin derivatives. It is usually obtained within the juice production in the food industries. In the current report, lemon pulp was initially dried and immediately after subjected to extraction with hot water and ethyl acetate. This study deals with the isolation of secondary metabolites along with determinations of 32 phenolic contents. After isolation of major components using Sephadex LH-20 column and silica gel, 11 phenolic components were totally characterized using the 1D and 2D NMR techniques. Lemon pulp known as citrus peel has been recognized as a potential source of coumarin, limonoids, and methoxylated flavones. The antioxidant activities of the limonin (LMN) and lemon pulp ethyl acetate extract (LPEA) were evaluated using in vitro methods involving total antioxidant activity, reducing power, inhibition of lipid peroxidation, scavenging activities of DPPH∙, H2O2 and ABTS+. and compared with standards at 10, 25, 50 and 100 μg.mL-1. According to the finding of this research, LMN and LPEA showed strong in vitro antioxidant activities and also were significantly exhibited an increase to be in a dose-dependent manner, (p <0.05). Manuscript profile -
Open Access Article
2 - Chemical constituents, quantitative analysis and insecticidal activities of plant extract and essential oil from Origanum onites L.
Ramazan Erenler Ibrahim Demirtas Tunay Karan Fatih Gul Omer Kayir Omer KarakocOriganum, widely used for food and pharmaceutical industry, belonging to the Lamiaceae family, is an aromatic and medicinal plant. The aerial parts of the Origanum onites L. were dried at shade and was extracted with methanol. Rosmarinic acid, gentisic acid, 4-hydroxybe MoreOriganum, widely used for food and pharmaceutical industry, belonging to the Lamiaceae family, is an aromatic and medicinal plant. The aerial parts of the Origanum onites L. were dried at shade and was extracted with methanol. Rosmarinic acid, gentisic acid, 4-hydroxybenzoic acid, protocatechuic acid, caffeic acid, vanillic acid, 4-hydroxylbenzaldehyde, p-cumaric acid, ferullic acid, apigenin-7-glucoside, and narigenin were found in O. onites methanol extract. Quantitative analyses of these compounds were determined by LC-TOF/MS and rosmarinic acid was the main constituent (32.05 mg/100 g dried plant). The essential oil was isolated by steam distillation and compounds were identified by GC-MS analysis. The essential oil included the carvacrol (88.71%) as a main product. p-Cymene (3.09%) was found as a second chief product. The essential oil exhibited excellent insecticidal activity on Sitophilus granaries and Sitophilus oryzae pests. However, methanol extract revealed the moderate activity on S. granarius pet. Manuscript profile
List of Articles I. Demirtas
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